PKC delta (PRKCD) (NM_212539) Human Untagged Clone

SKU SC116249

PRKCD (untagged)-Human protein kinase C, delta (PRKCD), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: PKC delta
• Synonyms: ALPS3; CVID9; MAY1; nPKC-delta; PKCD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_212539 edited; ATGGCGCCGTTCCTGCGCATCGCCTTCAACTCCTATGAGCTGGGCTCCCTGCAGGCCGAG; GACGAGGCGAACCAGCCCTTCTGTGCCGTGAAGATGAAGGAGGCGCTCAGCACAGAGCGT; GGGAAAACACTGGTGCAGAAGAAGCCGACCATGTATCCTGAGTGGAAGTCGACGTTCGAT; GCCCACATCTATGAGGGGCGCGTCATCCAGATTGTGCTAATGCGGGCAGCAGAGGAGCCA; GTGTCTGAGGTGACCGTGGGTGTGTCGGTGCTGGCCGAGCGCTGCAAGAAGAACAATGGC; AAGGCTGAGTTCTGGCTGGACCTGCAGCCTCAGGCCAAGGTGTTGATGTCTGTTCAGTAT; TTCCTGGAGGACGTGGATTGCAAACAGTCTATGCGCAGTGAGGACGAGGCCAAGTTCCCA; ACGATGAACCGCCGCGGAGCCATCAAACAGGCCAAAATCCACTACATCAAGAACCATGAG; TTTATCGCCACCTTCTTTGGGCAACCCACCTTCTGTTCTGTGTGCAAAGACTTTGTCTGG; GGCCTCAACAAGCAAGGCTACAAATGCAGGCAATGTAACGCTGCCATCCACAAGAAATGC; ATCGACAAGATCATCGGCAGATGCACTGGCACCGCGGCCAACAGCCGGGACACTATATTC; CAGAAAGAACGCTTCAACATCGACATGCCGCACCGCTTCAAGGTTCACAACTACATGAGC; CCCACCTTCTGTGACCACTGCGGCAGCCTGCTCTGGGGACTGGTGAAGCAGGGATTAAAG; TGTGAAGACTGCGGCATGAATGTGCACCATAAATGCCGGGAGAAGGTGGCCAACCTCTGC; GGCATCAACCAGAAGCTTTTGGCTGAGGCCTTGAACCAAGTCACCCAGAGAGCCTCCCGG; AGATCAGACTCAGCCTCCTCAGAGCCTGTTGGGATATATCAGGGTTTCGAGAAGAAGACC; GGAGTTGCTGGGGAGGACATGCAAGACAACAGTGGGACCTACGGCAAGATCTGGGAGGGC; AGCAGCAAGTGCAACATCAACAACTTCATCTTCCACAAGGTCCTGGGCAAAGGCAGCTTC; GGGAAGGTGCTGCTTGGAGAGCTGAAGGGCAGAGGAGAGTACTTTGCCATCAAGGCCCTC; AAGAAGGATGTGGTCCTGATCGACGACGACGTGGAGTGCACCATGGTTGAGAAGCGGGTG; CTGACACTTGCCGCAGAGAATCCCTTTCTCACCCACCTCATCTGCACCTTCCAGACCAAG; GACCACCTGTTCTTTGTGATGGAGTTCCTCAACGGGGGGGACCTGATGTACCACATCCAG; GACAAAGGCCGCTTTGAACTCTACCGTGCCACGTTTTATGCCGCTGAGATAATGTGTGGA; CTGCAGTTTCTACACAGCAAGGGCATCATTTACAGGGACCTCAAACTGGACAATGTGCTG; TTGGACCGGGATGGCCACATCAAGATTGCCGACTTTGGGATGTGCAAAGAGAACATATTC; GGGGAGAGCCGGGCCAGCACCTTCTGCGGCACCCCTGACTATATCGCCCCTGAGATCCTA; CAGGGCCTGAAGTACACATTCTCTGTGGACTGGTGGTCTTTCGGGGTCCTTCTGTACGAG; ATGCTCATTGGCCAGTCCCCCTTCCATGGTGATGATGAGGATGAACTCTTCGAGTCCATC; CGTGTGGACACGCCACATTATCCCCGCTGGATCACCAAGGAGTCCAAGGACATCCTGGAG; AAGCTCTTTGAAAGGGAACCAACCAAGAGGCTGGGAGTGACGGGAAACATCAAAATCCAC; CCCTTCTTCAAGACCATAAACTGGACTCTGCTGGAAAAGCGGAGGTTGGAGCCACCCTTC; AGGCCCAAAGTGAAGTCACCCAGAGACTACAGTAACTTTGACCAGGAGTTCCTGAACGAG; AAGGCGCGCCTCTCCTACAGCGACAAGAACCTCATCGACTCCATGGACCAGTCTGCATTC; GCTGGCTTCTCCTTTGTGAACCCCAAATTCGAGCACCTCCTGGAAGATTGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_212539 unedited; CACCCATTCCGCACGAGGCCGCGGCGCCGGAGCCCGAGGCGGCTGTAGCCCACATCTCCC; GATCGACCCCCGGCGCCCGCCCGCCGCGCGGAGGCCCGGGCCACACCTCACTGGCCGCTT; GGCCCATCCCAGTCAGCGCCGCGCCGAACCCCGTCCGCGCGCGCCGGGGAGCGGCTTTCT; CTTCGCTGCCGCCGCGACCCTTGGCGCCTGCCCCTGCAACGGGAGCCCCACTGCAGGCCC; CACCATGGCGCCGTTCCTGCGCATCGCCTTCAACTCCTATGAGCTGGGCTCCCTGCAGGC; CGAGGACGAGGCGAACCAGCCCTTCTGTGCCGTGAAGATGAAGGAGGCGCTCAGCACAGA; GCGTGGGAAAACACTGGTGCAGAAGAAGCCGACCATGTATCCTGAGTGGAAGTCGACGTT; CGATGCCCACATCTATGATGGGCGCGTCATCCAGATTGTGCTAATGCGGGCAGCAGAGGA; GCCAGTGTCTGAGGTGACCGTGGGTGTGTCGGTGCTGGCCGAGCGCTGCAAGAAGAACAA; TGGCAAGGCTGAGTTCTGGCTGGACCTGCAGCCTCAGGCCAAGGTGTTGATGTCTGTTCA; GTATTTCCTGGAGGACGTGGATTGCAAACAGTCTATGCGCAGTGAGGACGATGCCAAGTT; CCCAACGATGAACCGCCGCGGAGCCATCAAACAGGCCAAAATCCACTACATCAAGAACCA; TGAGTTTATCGCCACCTTCTTTGGGCAACCCACCTTCTGTTCTGTGTGCAAAGACTTTGT; CTGGGGCCCTCAACAAGCAAGGCTACAAATGCAAGCNATGTNACGCTGCCATCACAAGAA; ATGCATCGACCAGATCATCGGCAGATGCAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_212539 unedited; CAATCNANAGTCGAGTTTTTTTTTTTTTTTTTTGTCATATATATATTTTTTTGAAAAAGA; CAACATATCACCAGTCCCCCATGGTTTTTCTCTACTGAGCAATATATACAGCCTATTATA; TATATATATATGTCTATGTATATAATATATATTTTCAATAGATAACTACATTCAAGTAAT; GAAACACAGGATTTACAATTTCCCTCCGAAGGCAGAGGAAAAGCAGATTCACACACAGTT; CACAGAGGAAGTACCATGAGAGCCCGGCCAGACGGCAGCCAAGGACGCTCTGGGGGCAGG; GGCGGGGCCAGCAGCAGAAGTCACCACAGTCCCACTGGTGCTTATCGTGGGGAGCGGGCA; TGTGTGGGTGGAGGGCACGGCTAGCCTGATCTGTCCAGGAACCTCAATCTTCCAGGAGGT; GCTCGAATTTGGGGTTCACAAAGGAGAACCCAGCGAATGCACACTGGTCCATGGAGCCGA; TGAGGTTCTTGTCGCTGTAAGAGAGGCGCGCCTTCTTCCTCACGAACTCCCGGTCAAAGT; TACCGTAGCCTCTGGGGGACTTCACTTTGGGCCCTGAAGGCGGCTCCAACCTCCCCTCTT; TCATCATATTCCACCCCATCGCCCCCCAGACGGCGCGGACTCTGATGTTTCCCGCACTCC; CATTCTCTTCGGCGGGCCCCCCTTTACACAGCCTTCCTCATAACTTTCTTTGCACCCCCC; GCCGACCCACTCCGGACAATGTGGTTGCGTTCCCCCTCCTCGCCCCACACTAGTATCTCC; ATATATTTATCCCAGAATCGGGAACCGCCCCCCCCCTCTTTCTTTTCCTCCCCCTGTTTT; ATCCCGTTTCTCTTATTCCCCCCCGCCCCCCCTGCTTCCCCCGTTCTATTTTTTCTCTCC; ATCCGCCCCCTCCCGCTTTCCTCTTCCCCCCTATTCCCCCCGTACCCCCGCTTGTCCCCT; CCCGACCTTTCCATTTTATTTCCCCTCCCCCCCCCCTCTTCCTTTTCGCCCCCCG;
• Restriction Sites: NotI-NotI
• ACCN: NM_212539
• Insert Size: 2820 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_212539.1, NP_997704.1
• RefSeq Size: 2738 bp
• RefSeq ORF: 2031 bp
• Locus ID: 5580
• UniProt ID: Q05655
• Cytogenetics: 3p21.1
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, GnRH signaling pathway, Neurotrophin signaling pathway, Tight junction, Type II diabetes mellitus, Vascular smooth muscle contraction
• Summary: The protein encoded by this gene is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrome. [provided by RefSeq, Aug 2017]; Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 4. The resulting isoform (c) is shorter at the N-terminus compared to isoform a. Variants 1, 2, 3, 6, and 7 all encode the same isoform (c).