Nociceptin (PNOC) (NM_006228) Human Untagged Clone

SKU SC116235

PNOC (untagged)-Human prepronociceptin (PNOC)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Nociceptin
• Synonyms: N/OFQ; NOP; OFQ; ppN/OFQ; PPNOC
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116235 sequence for NM_006228 edited (data generated by NextGen Sequencing); ATGAAAGTCCTGCTTTGTGACCTGCTGCTGCTCAGTCTCTTCTCCAGTGTGTTCAGCAGT; TGTCAGAGGGACTGTCTCACATGCCAGGAGAAGCTCCACCCAGCCCTGGACAGCTTCGAC; CTGGAGGTGTGCATCCTCGAGTGTGAAGAGAAGGTCTTCCCCAGCCCCCTCTGGACTCCA; TGCACCAAGGTCATGGCCAGGAGCTCTTGGCAGCTCAGCCCTGCCGCCCCAGAGCATGTG; GCGGCTGCTCTCTACCAGCCGAGAGCTTCGGAGATGCAGCATCTGCGGCGAATGCCCCGA; GTCCGGAGCTTGTTCCAGGAGCAGGAAGAGCCCGAGCCTGGCATGGAGGAGGCTGGTGAG; ATGGAGCAGAAGCAGCTGCAGAAGAGATTTGGGGGCTTCACCGGGGCCCGGAAGTCGGCC; AGGAAGTTGGCCAATCAGAAGCGGTTCAGTGAGTTTATGAGGCAATACTTGGTCCTGAGC; ATGCAGTCCAGCCAGCGCCGGCGCACCCTGCACCAGAATGGTAATGTGTAG; ; Clone variation with respect to NM_006228.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006228 unedited; NGGGCACCATTTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACGAGGCCTCGTGC; CGAATTCGGCACGAGGATCGGAGGGAGCCGGGGACTGACAGCTCTCAGCACCTGCTTCCT; GCTCCTGCACCATGAAAGTCCTGCTTTGTGACCTGCTGCTGCTCAGTCTCTTCTCCAGTG; TGTTCAGCAGTTGTCAGAGGGACTGTCTCACATGCCAGGAGAAGCTCCACCCAGCCCTGG; ACAGCTTCGACCTGGAGGTGTGCATCCTCGAGTGTGAAGAGAAGGTCTTCCCCAGCCCCC; TCTGGACTCCATGCACCAAGGTCATGGCCAGGAGCTCTTGGCAGCTCAGCCCTGCCGCCC; CAGAGCATGTGGCGGCTGCTCTCTACCAGCCGAGAGCTTCGGAGATGCAGCATCTGCGGC; GAATGCCCCGAGTCCGGAGCTTGTTCCAGGAGCAGGAAGAGCCCGAGCCTGGCATGGAGG; AGGCTGGTGAGATGGAGCAGAAGCAGCTGCAGAAGAGATTTGGGGGCTTCACCGGGGCCC; GGAAGTCGGCCAGGAAGTTGGCCAATCAGAAGCGGTTCAGTGAGTTTATGAGGCAATACT; TGGTCCTGAGCATGCAGTCCAGCCAGCGCCGGCGCACCCTGCACCAGAATGGTAATGTGT; AGCCGGAAGGGGCGCTCCTCCCAGCTGTACCGGCCACTGCAACCCATGAGCGTTCAGGTG; ATCCCCCAACAGCATGTGCTCAGCCCCAGACCTGCCGCCTGGGAATCAGGATTCCTTTTT; TCCCCAAGCCACTGAGCGCCTGCAGATTCCGCCAGGCCTTGTTTGCCTCCANAACCTTCC; CTATTGATTGTTTCTCCCCACCCCCTTGCATGTTTACCACAAACCTGTTGCTACATAT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006228 unedited; GCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTAACCATTTGCTCAGTAGAAGTTTA; ATGGAGAAATCGTTGTTTAAAACAATCAGTCAAAAAGAACAGCTCTTTTACAAACAAGTT; ATGGCAGTGGCAAGTCAAAACCCCAGGTTCAATTTCCTATTCCTTTCACCTGCCCCTAGA; AGGGGCAAGAGGCGGGTGAGCAGGAGAGATGGGGCTATTGAAATGGTAGCTAGAGGAATT; ACAAAAATACACTCTGATGTAGCAACAGGGTTGTGGTGAAACATGCCAGGGGGCTGGGGA; GGAACAATCAGACGGGAAGGTTCTGGAGGCAAACGAAGCCTGCGGGATCTGCAGGCGCTC; AGTGCCTTGGGGAAGAAGGAATCCTGATTCCCAGGCGGCAGGTCTGGGGCTGAGCACATG; CTGTTTGGGGGATCACCTGGACGCTCATGGGTTGCAGTGGCCGGTACAGCTGGGAGGAGC; GCCCCTTCCGGCTACACATTACCATTCTGGTGCAGGGTGCGCCGGCGCTGGCTGGACTGC; ATGCTCAGGACCAAGTATTGCCTCATAAACTCACTGAACCGCTTCTGATTGGCCAACTTC; CTGGCCGACTTCCGGGCCCCGGTGAAGCCCCCAAATCTCTTCTGCAGCTGCTTCTGCTCC; ATCTCACCAGCCTNCTCCATGCCCAGCTCGGGCTCTTCCTGCTCCTGGAAAACAGCTCNG; ACTCGGGGCATCTCGCGCAGATGCTGCATCTCCGAAACTCTCGGCTGTAAAAGACAACCG; CCACATGCTCTTGGGCGGCAAGGCTGACCTCCAAGACCTCCTGGCCTGACCTTGGGCCAT; GAGCCAAAGGGGCCTGGGAAAACCTCTCTTCCACTTGAGATGCCACCCCCAGTCAAACTG; CCACAGCTGGGGGGACTTCTCCGGCCGGTAACACGCCCTTTGACACGGNTGAACCTGGAA; AAAAACGTCCCACCCGTCCAACCGGCCTTCTTGGGCGACG;
• Restriction Sites: NotI-NotI
• ACCN: NM_006228
• Insert Size: 1110 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006228.2, NP_006219.1
• RefSeq Size: 1198 bp
• RefSeq ORF: 531 bp
• Locus ID: 5368
• UniProt ID: Q13519
• Cytogenetics: 8p21.1
• Domains: Opiods_neuropep
• Protein Families: Secreted Protein
• Summary: This gene encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include nociceptin, nocistatin, and orphanin FQ2 (OFQ2). Nociceptin, also known as orphanin FQ, is a 17-amino acid neuropeptide that binds to the nociceptin receptor to induce increased pain sensitivity, and may additionally regulate body temperature, learning and memory, and hunger. Another product of the encoded preproprotein, nocistatin, may inhibit the effects of nociceptin. [provided by RefSeq, Jul 2015]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.