Semaphorin 3c (SEMA3C) (NM_006379) Human Untagged Clone

SKU SC116160

SEMA3C (untagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C (SEMA3C)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Semaphorin 3c
• Synonyms: SEMAE; SemE
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006379 edited; GAATTCGGCACGAGACTGCGAAAGGAGCAGGGTTGCGGAGCTAGGGCTCCAGCCTGCGGC; CGCGCATTCTTGCGTCTGGCCAGCCGCGAGCTCTAAGGGTCGGCCCCGCCCGGTCCGCCC; CCGCGGCTCCCTGCCAGGCTCTCGCGGGCGCGCTCGGGGTGGGGCCTCGCGGCTGGCGGA; GATGCGGCCGGGGCTGCGCGGTGGTGATGCGAGCCTGCTGGGCGGCGCGCCGGGGCAGCC; GGAGCCGCGCGCCGCGGCGCTGTAATCGGACACCAAGAGCGCTCGCCCCCGGCCTCCGGC; CACTTTCCATTCACTCCGAGGTGCTTGATTGAGCGACGCGGAGAAGAGCTCCGGGTGCCG; CGGCACTGCAGCGCTGAGATTCCTTTACAAAGAAACTCAGAGGACCGGGAAGAAAGAATT; TCACCTTTGCGACGTGCTAGAAAATAAGGTCGTCTGGGAAAAGGACTGGAGACACAAGCG; CATCCAACCCCGGTAGCAAACTGATGACTTTTCCGTGCTGATTTCTTTCAACCTCGGTAT; TTTCCCTTGGATATTAACTTGCATATCTGAAGAAATGGCATTCCGGACAATTTGCGTGTT; GGTTGGAGTATTTATTTGTTCTATCTGTGTGAAAGGATCTTCCCAGCCCCAAGCAAGAGT; TTATTTAACATTTGATGAACTTCGAGAAACCAAGACCTCTGAATACTTCAGCCTTTCCCA; CCATCCTTTAGACTACAGGATTTTATTAATGGATGAAGATCAGGACCGGATATATGTGGG; AAGCAAAGATCACATTCTTTCCCTGAATATTAACAATATAAGTCAAGAAGCTTTGAGTGT; TTTCTGGCCAGCATCTACAATCAAAGTTGAAGAATGCAAAATGGCTGGCAAAGATCCCAC; ACACGGCTGTGGGAACTTTGTCCGTGTAATTCAGACTTTCAATCGCACACATTTGTATGT; CTGTGGGAGTGGCGCTTTCAGTCCTGTCTGTACTTACTTGAACAGAGGGAGGAGATCAGA; GGACCAAGTTTTCATGATTGACTCCAAGTGTGAATCTGGAAAAGGACGCTGCTCTTTCAA; CCCCAACGTGAACACGGTGTCTGTTATGATCAATGAGGAGCTTTTCTCTGGAATGTATAT; AGATTTCATGGGGACAGATGCTGCTATTTTTCGAAGTTTAACCAAGAGGAATGCGGTCAG; AACTGATCAACATAATTCCAAATGGCTAAGTGAACCTATGTTTGTAGATGCACATGTCAT; CCCAGATGGTACTGATCCAAATGATGCTAAGGTGTACTTCTTCTTCAAAGAAAAACTGAC; TGACAATAACAGGAGCACGAAACAGATTCATTCCATGATTGCTCGAATATGTCCTAATGA; CACTGGTGGACTGCGTAGCCTTGTCAACAAGTGGACCACTTTCTTAAAGGCGAGGCTGGT; GTGCTCGGTAACAGATGAAGACGGCCCAGAAACACACTTTGATGAATTAGAGGATGTGTT; TCTGCTGGAAACTGATAACCCGAGGACAACACTAGTGTATGGCATTTTTACAACATCAAG; CTCAGTTTTCAAAGGATCAGCCGTGTGTGTGTATCATTTATCTGATATACAGACTGTGTT; TAATGGGCCTTTTGCCCACAAAGAAGGGCCCAATCATCAGCTGATTTCCTATCAGGGCAG; AATTCCATATCCTCGCCCTGGAACTTGTCCAGGAGGAGCATTTACACCCAATATGCGAAC; CACCAAGGAGTTCCCAGATGATGTTGTCACTTTTATTCGGAACCATCCTCTCATGTACAA; TTCCATCTACCCAATCCACAAAAGGCCTTTGATTGTTCGTATTGGCACTGACTACAAGTA; TACAAAGATAGCTGTGGATCGAGTGAACGCTGCTGATGGGAGATACCATGTCCTGTTTCT; CGGAACAGATCGGGGTACTGTGCAAAAAGTGGTTGTTCTTCCTACTAACAACTCTGTCAG; TGGCGAGCTCATTCTGGAGGAGCTGGAAGTCTTTAAGAATCATGCTCCTATAACAACAAT; GAAAATTTCATCTAAAAAGCAACAGTTGTATGTGAGTTCCAATGAAGGGGTTTCCCAGGT; ATCTCTGCACCGCTGCCACATCTATGGTACAGCCTGTGCTGACTGCTGCCTGGCGCGGGA; CCCTTATTGCGCCTGGGATGGCCATTCCTGTTCCAGATTCTACCCAACTGGGAAACGGAG; GAGCCGAAGACAAGATGTGAGACATGGAAACCCACTGACTCAATGCAGAGGATTTAATCT; AAAAGCATACAGAAATGCAGCTGAAATTGTGCAGTATGGAGTAAAAAATAACACCACTTT; TCTGGAGTGTGCCCCCAAGTCTCCGCAGGCATCTATCAAGTGGCTGTTACAGAAAGACAA; AGACAGGAGGAAAGAGGTTAAGCTGAATGAACGAATAATAGCCACTTCACAGGGACTCCT; GATCCGCTCTGTTCAGGGTTCTGACCAAGGACTTTATCACTGCATTGCTACAGAAAATAG; TTTCAAGCAGACCATAGCCAAGATCAACTTCAAAGTTTTAGATTCAGAAATGGTGGCTGT; TGTGACGGACAAATGGTCCCCGTGGACCTGGGCCAGCTCTGTGAGGGCTTTACCCTTCCA; CCCGAAGGACATCATGGGGGCATTCAGCCACTCAGAAATGCAGATGATTAACCAATACTG; CAAAGACACTCGGCAGCAACATCAGCAGGGAGATGAATCACAGAAAATGAGAGGGGACTA; TGGCAAGTTAAAGGCCCTCATCAATAGTCGGAAAAGTAGAAACAGGAGGAATCAGTTGCC; AGAGTCATAATATTTTCTTATGTGGGTCTTATGCTTCCATTAACAAATGCTCTGTCTTCA; ATGATCAAATTTTGAGCAAAGAAACTTGTGCTTTACCAAGGGGAATTACTGAAAAAGGTG; ATTACTCCTGAAGTGAGTTTTACACGAACTGAAATGAGCATGCATTTTCTTGTATGATAG; TGACTAGCACTAGACATGTCATGGTCCTCATGGTGCATATAAATATATTTAACTTAACCC; AGATTTTATTTATATCTTTATTCACCTTTTCTTCAAAATCGATATGGTGGCTGCAAAACT; AGAATTGTTGCATCCCTCAATTGAATGAGGGCCATATCCCTGTGGTATTCCTTTCCTGCT; TTGGGGCTTTAGAATTCTAATTGTCAGTGATTTTGTATATGAAAACAAGTTCCAAATCCA; CAGCTTTTACGTAGTAAAAGTCATAAATGCATATGACAGAATGGCTATCAAAAGAAATAG; AAAAGGAAGACGGCATTTAAAGTTGTATAAAAACACGAGTTATTCATAAAGAGAAAATGA; TGAGTTTTTATGGTTCCAATGAAATATGTTGGGGTTTTTTTAAGATTGTAAAAATAATCA; GTTACTGGTATCTGTCACTGACCTTTGTTTCCTTATTCAGGAAGATAAAAATCAGTAACC; TACCCCATGAAGATATTTGGTGGGAGTTATATCAGTGAAGCAGTTTGGTTTATATTCTTA; TGTTATCACCTTCCAAACAAAAGCACTTACTTTTTTTGGAAGTTATTTAATTTATTTTAG; ACTCAAAGAATATAATCTTGCACTACTCAGTTATTACTGTTTGTTCTCTTATTCCCTAGT; CTGTGTGGCAAATTAAACAATATAAGAAGGAAAAATTTGAAGTATTAGACTTCTAAATAA; GGGGTGAAATCATCAGAAAGAAAAATCAAAGTAGAAACTACTAATTTTTTAAGAGGAATT; TATAACAAATATGGCTAGTTTTCAACTTCAGTACTCAAATTCAATGATTCTTCCTTTTAT; TAAAACCAGTCTCAGATATCATACTGATTTTTAAGTCAACACTATATATTTTATGATCTT; TTCAGTGTGATGGCAAGGTGCTTGTTATGTCTAGAAAGTAAGAAAACAATATGAGGAGAC; ATTCTGTCTTTCAAAAGGTAATGGTACATACGTTCACTGGTCTCTAAGTGTAAAAGTAGT; AAATTTTGTGATGAATAAAATAATTATCTCCTAATTGTATGTTAGAATAATTTTATTAGA; ATAATTTCATACTGAAATTATTTTCTCCAAATAAAAATTAGATGGAAAAATGTGAAAAAA; ATTATTCATGCTCTCATATATATTTTAAAAACACTACTTTTGCTTTTTTATTTACCTTTT; AAGACATTTTCATGCTTCCAGGTAAAAACAGATATTGTACCATGTACCTAATCCAAATAT; CATATAAACATTTTATTTATAGTTAATAATCTATGATGAAGGTAATTAAAGTAGATTATG; GCCTTTTTAAGTATTGCAGTCTAAAACTTCAAAAACTAAAATCATTGTCAAAATTAATAT; GATTATTAATCAGAATATCAGAATATGATTCACTATTTAAACTATGATAAATTATGATAA; TATATGAGGAGGCCTCGCTATAGCAAAAATAGTTAAAATGCTGACATAACACCAAACTTC; ATTTTTTAAAAAATCTGTTGTTCCAAATGTGTATAATTTTAAAGTAATTTCTAAAGCAGT; TTATTATAATGGTTTGCCTGCTTAAAAGGTATAATTAAACTTCTTTTCTCTTCTACATTG; ACACACAGAAATGTGTCAATGTAAAGCCAAAACCATCTTCTGTGTTTATGGCCAATCTAT; TCTCAAAGTTAAAAGTAAAATTGTTTCAGAGTCACAGTTCCCTTTATTTCACATAAGCCC; AAACTGATAGACAGTAACGGTGTTTAGTTTTATACTATATTTGTGCTATTTAATTCTTTC; TATTTTCACAATTATTAAATTGTGTACACTTTCATTACTTTTAAAAATGTAGAAATTCTT; CATGAACATAACTCTGCTGAATGTAAAAGAAAATTTTTTTTCAAAAATGCTGTTAATGTA; TACTACTGGTGGTTGATTGGTTTTATTTTATGTAGCTTGACAATTCAGTGACTTAATATC; TATTCCATTTGTATTGTACATAAAATTTTCTAGAAATACACTTTTTTCCAAAGTGTAAGT; TTGTGAATAGATTTTAGCATGATGAAACTGTCATAATGGTGAATGTTCAATCTGTGTAAG; AAAACAAACTAAATGTAGTTGTCACACTAAAATTTAATTGGATATTGATGAAATCATTGG; CCTGGCAAAATAAAACATGTTGAATTCCCCAAAAAAAAAAAAAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006379 unedited; TAATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGACTGCGAAAGGA; GCAGGGTTGCGGAGCTAGGGCTCCAGCCTGCGGCCGCGCATTCTTGCGTCTGGCCAGCCG; CGAGCTCTAAGGGTCGGCCCCGCCCGGTCCGCCCCCGCGGCTCCCTGCCAGGCTCTCGCG; GGCGCGCTCGGGGTGGGGCCTCGCGGCTGGCGGAGATGCGGCCGGGGCTGCGCGGTGGTG; ATGCGAGCCTGCTGGGCGGCGCGCCGGGGCAGCCGGAGCCGCGCGCCGCGGCGCTGTAAT; CGGACACCAAGAGCGCTCGCCCCCGGCCTCCGGCCACTTTCCATTCACTCCGAGGTGCTT; GATTGAGCGACGCGGAGAAGAGCTCCGGGTGCCGCGGCACTGCAGCGCTGAGATTCCTTT; ACAAAGAAACTCAGAGGACCGGGAAGAAAGAATTTCACCTTTGCGACGTGCTAGAAAATA; AGGTCGTCTGGGAAAAGGACTGGAGACACAAGCGCATCCAACCCCGGTAGCAAACTGATG; ACTTTTCCGTGCTGATTTCTTTCAACCTCGGTATTTTCCCTTGGATATTAACTTGCATAT; CTGAAGAAATGGCATTCCGGACAATTTGCGTGTTGGTTGGAGTATTTATTTGTTCTATCT; GTGTGAAAGGATCTTCCCAGCCCCAAGCAAGAGTTTATTTAACATTTGATGAACTTCGAG; AAACCAAGACCTCTGAATACTTCAGCCTTTCCCACCATCCTTTAGACTACAGGATTNTAT; TAATGGATGAAGATCAGGACCGGATATATGTGGGAAGCAAAGATCACNATTCTNTNCCCT; GATATTAACAATATAAGTCAAGAAGCCTTGAGTGTTNTCTGGCCAGCATCTACAATCAAA; GTTGAGAATGCAAAATGGCTGGNCAAGATCCCACACACGCTGTGGNAAACTTGTNCCGTG; TATCAGACTTTCATCGACACATTGNAGTCTGTGGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006379 unedited; TTGTACGCGGCCGCATCTATAGTCGGTTTTTTTTTTTTTTTTTTTTTTGGGGAATTCAAC; ATGTTTTATTTTGCCAGGCCAATGATTTCATCAATATCCAATTAAATTTTAGTGTGACAA; CTACATTTAGTTTGGTTTCTTACACAGATTGAACATTCACCATTATGACAGTTTCATCAT; GCTAAAATCTATTCACAAACTTACACTTTGGAAAAAAGTGTATTTCTAGAAAATTTTATG; TACAATACAAATGGAATAGATATTAACCGACTGAATTGACCAGCTCCCTAACATAACACG; CCTGGACCACCAATTGTTTGCGTTGACAGGGTTGCTGAAAAATAACCCCCTTTTACCTTC; TACCTCACCCTGCTCTTGACCAACCTCCCCCTTCCCACCCACCCCGCCAGCGCCCCCCCC; CCTCCCTCTGCCCACTCTTCATGAATTTCGCCTTCCTTCCCATCCTCAAACCTACCCTCA; GTCCCGCCAATCATTCCATCCACTTGCACTCTTCTGGCCCCCGTCACCCTCCCACCCTTC; CCCCCCTCCAACTCCCAACATCACGCTCTCCCCCACGCCTACCTCTTCCTGCCCCCTCTC; ACTTTTTTTTCTTCTCCCTTATCCCCTCCGCTGCTCCCATTCTTCTTTTACATTACTACC; ATCTACCCCACTAACCCCCTCCCCCCTCCGTCACCCGACCTCCCTCCCCCCCCTCGACCG; CCCCGGTAATGATCGTCTTTTTTCCCTCATCCAACCACCTCGCCCCCTCTCTCCTTTTCT; TTTCTCCTCACCCCCACCCTCCCACATCCCACCTCCACCCTCTCCCCTCTCCCTCCACCG; TTACCTCTTAGCCCCACGCGATGCTCTCTCCCCTATCCTCTACCGACTACCTATCTCTTC; CTCCACTAATTTCCTCCCGATCGCGCACACCCGAGTATANGAGATTTTATAACTTTATTC; GATATCCCGCCTCTCCGTCCATCTATTCCCGCCTCCCCCGCCG;
• Restriction Sites: NotI-NotI
• ACCN: NM_006379
• Insert Size: 5150 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006379.2, NP_006370.1
• RefSeq Size: 5189 bp
• RefSeq ORF: 2256 bp
• Locus ID: 10512
• UniProt ID: Q99985
• Cytogenetics: 7q21.11
• Domains: IG, PSI, Sema
• Protein Families: Secreted Protein
• Protein Pathways: Axon guidance
• Summary: This gene encodes a secreted glycoprotein that belongs to the semaphorin class 3 family of neuronal guidance cues. The encoded protein contains an N-terminal sema domain, integrin and immunoglobulin-like domains, and a C-terminal basic domain. Homodimerization and proteolytic cleavage of the C-terminal propeptide are necessary for the function of the encoded protein. It binds a neuropilin co-receptor before forming a heterotrimeric complex with an associated plexin. An increase in the expression of this gene correlates with an increase in cancer cell invasion and adhesion. Naturally occurring mutations in this gene are associated with Hirschsprung disease. [provided by RefSeq, May 2017]; Transcript Variant: This variant (2) contains an alternate exon in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1.