PPIH (NM_006347) Human Untagged Clone

SKU SC116136

PPIH (untagged)-Human peptidylprolyl isomerase H (cyclophilin H) (PPIH)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PPIH
• Synonyms: CYP-20; CYPH; SnuCyp-20; USA-CYP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_006347 edited; ATGGCGGTGGCAAATTCAAGTCCTGTTAACCCCGTGGTGTTCTTTGATGTCAGTATTGGC; GGTCAGGAAGTTGGCCGCATGAAGATCGAGCTCTTTGCAGACGTTGTGCCTAAGACGGCC; GAGAACTTTAGGCAGTTCTGCACCGGAGAATTCAGGAAAGATGGGGTTCCAATAGGATAC; AAAGGAAGCACCTTCCACAGGGTCATAAAGGATTTCATGATTCAGGGTGGAGATTTTGTT; AATGGAGATGGTACTGGAGTCGCCAGTATTTACCGGGGGCCATTTGCAGATGAAAATTTT; AAACTTAGACACTCAGCTCCAGGCCTGCTTTCCATGGCGAACAGTGGTCCAAGTACAAAT; GGCTGTCAGTTCTTTATCACCTGCTCTAAGTGCGATTGGCTGGATGGGAAGCATGTGGTG; TTTGGAAAAATCATCGATGGACTTCTAGTGATGAGAAAGATTGAGAATGTTCCCACAGGC; CCCAACAATAAGCCCAAGCTACCTGTGGTGATCTCGCAGTGTGGGGAGATGTAG;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006347 unedited; NGGGGGGNNNNNNNNNNNNNNCCCCNNNNCCGGTTCAGAATTTGTNATACGACTCATATA; GGCGGCCGCGNAATTCGCACGAGCATGGCGGTGGCAAATTCAAGTCCTGTTAACCCCGTG; GTGTTCTTTGATGTCAGTATTGGCGGTCAGGAAGTTGGCCGCATGAAGATCGAGCTCTTT; GCAGACGTTGTGCCTAAGACGGCCGAGAACTTTAGGCAGTTCTGCACCGGAGAATTCAGG; AAAGATGGGGTTCCAATAGGATACAAAGGAAGCACCTTCCACAGGGTCATAAAGGATTTC; ATGATTCAGGGTGGAGATTTTGTTAATGGAGATGGTACTGGAGTCGCCAGTATTTACCGG; GGGCCATTTGCAGATGAAAATTTTAAACTTAGACACTCAGCTCCAGGCCTGCTTTCCATG; GCGAACAGTGGTCCAAGTACAAATGGCTGTCAGTTCTTTATCACCTGCTCTAAGTGCGAT; TGGCTGGATGGGAAGCATGTGGTGTTTGGAAAAATCATCGATGGACTTCTAGTGATGAGA; AAGATTGAGAATGTTCCCACAGGCCCCAACAATAAGCCCAAGCTACCTGTGGTGATCTCG; CAGTGTGGGGAGATGTAGTCCAGACAAAGACTGAATCANGCCTTCCCTTCTTCTTNGGTG; TGTTCTTGAGTAAGAATATCTGGACTGGCCCCCGTCTTTGCTTCCCTGCCTGCTGCTGCC; CCATTNTGATCAGAGACATGGNAGTGTCAGAGATTCAGAATCCCAGATTGTCTTTTTAGT; TTCAAACTGTAATAAAGTTTTTTTGTATGCGTAAAAAAAAAAAAAAAAACTCGACTCTAG; ATTGCGGCCCGCGTCATAGCTGTTTCCTGAACAGATCCCNGNNTGGCATCCNTGTGACCC; CTCCCCATGCCTNTTCTGGCCCTGGNAGTGCCACTCCATGCCCACCACCTTTTGTCTATA; AAATNAGNTGCATCATTNTGNCTGACTNAGNGTCCTCTATATATATGGNTGAGGGGGGGG; GGTTTTATTACAAGGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006347 unedited; AGTTAAAATCTTTTTTTTTTTACGCATACAAAAAAACTTTATTTACAGTGTGAAAACTTA; AAGACAATCTTGGATTCTGAATCTCTGACACTTCCATGGTCTCTTGATCAAATGGGGCAG; CAGCAGGCAGGGAAGCAAAGACGGGGGCCAGTCCAGATTATCTTACTCAAGAACACCACC; AAGAAGAAGGGAAGGCCTGATTCAGTCTTTGTCTGGACTACATCTCCCCACACTGCGAGA; TCACCACAGGTAGCTTGGGCTTATTGTTGGGGCCTGTGGGAACATTCTCAATCTTTCTCA; TCACTAGAAGTCCATCGATGATTTTTCCAAACACCACATGCTTCCCATCCAGCCAATCGC; ACTTAGAGCAGGTGATAAAGAACTGACAGCCATTTGTACTTGGACCACTGTTCGCCATGG; AAAGCAGGCCTGGAGCTGAGTGTCTAAGTTTAAAATTTTCATCTGCAAATGGCCCCCGGT; AAATACTGGCGACTCCAGTACCATCTCCATTAACAAAATCTCCACCCTGAATCATGAAAT; CCTTTATGACCCTGTGGAAGGTGCTTCCTTTGTATCCTATTGGAACCCCATCTTTCCTGA; ATTCTCCGGTGCAGAACTGCCTAAAGTTCTCGGCCGTCTTATGCACAACGTCTGCAAAGA; GCTCGATCTTCATGCCGCCAACTTCCTGACCGCCAATACTGACATCAAAGAACACCACGG; GGTTAACAGGACTTGAATTTGCCACCGNCATGCTCGTGCCGAATTCGCGCCGCCCTATAT; GAGTCGTATTACAAATTCTGACGTTCACTAAACGAGCTCTGCTATTAGACCTCCCACGTC; A;
• Restriction Sites: NotI-NotI
• ACCN: NM_006347
• Insert Size: 800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006347.3, NP_006338.1
• RefSeq Size: 813 bp
• RefSeq ORF: 534 bp
• Locus ID: 10465
• UniProt ID: O43447
• Cytogenetics: 1p34.2
• Domains: pro_isomerase
• Protein Pathways: Spliceosome
• Summary: The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is a specific component of the complex that includes pre-mRNA processing factors PRPF3, PRPF4, and PRPF18, as well as U4/U5/U6 tri-snRNP. This protein has been shown to possess PPIase activity and may act as a protein chaperone that mediates the interactions between different proteins inside the spliceosome. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) encodes the longer isoform (1).