PRPF8 (NM_006445) Human Untagged Clone
CAT#: SC116070
PRPF8 (untagged)-Human PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae) (PRPF8)
"NM_006445" in other vectors (3)
Product Images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Symbol | PRPF8 |
Synonyms | HPRP8; PRP8; PRPC8; RP13; SNRNP220 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_006445, the custom clone sequence may differ by one or more nucleotides
ATGGCCGGAGTGTTTCCTTATCGAGGGCCGGGTAACCCGGTGCCTGGCCCTCTAGCCCCGCTACCGGACT ACATGTCGGAGGAGAAGCTGCAGGAGAAAGCTCGAAAATGGCAGCAATTGCAGGCCAAGCGCTATGCAGA AAAGCGGAAGTTTGGGTTTGTGGATGCCCAGAAGGAAGACATGCCCCCAGAACATGTCAGGAAGATCATT CGAGACCATGGAGACATGACCAACAGGAAGTTCCGCCATGACAAAAGGGTTTACTTGGGTGCCCTAAAGT ACATGCCCCACGCAGTCCTCAAACTCCTGGAGAACATGCCTATGCCTTGGGAGCAGATTCGGGATGTGCC TGTGCTGTACCACATCACTGGAGCCATTTCCTTCGTCAATGAGATTCCCTGGGTCATTGAACCTGTCTAC ATCTCCCAGTGGGGGTCAATGTGGATTATGATGCGCCGAGAAAAAAGAGATAGGAGGCATTTCAAGAGGA TGCGTTTTCCCCCTTTTGATGATGAGGAGCCGCCCTTGGACTATGCTGACAACATCCTAGATGTTGAGCC ACTGGAGGCCATTCAGCTAGAGCTGGACCCTGAGGAGGACGCCCCTGTGTTGGACTGGTTCTATGACCAC CAGCCGTTGAGGGACAGCAGGAAGTATGTAAATGGCTCCACTTACCAGCGCTGGCAGTTCACACTACCTA TGATGTCGACTCTCTACCGCCTGGCTAATCAGCTCCTGACAGACTTGGTGGATGACAACTACTTCTACCT GTTTGATTTGAAGGCCTTCTTTACGTCCAAGGCACTCAATATGGCCATTCCTGGAGGCCCCAAATTTGAA CCTCTTGTTCGAGACATCAACCTACAGGATGAAGACTGGAATGAATTCAATGATATTAACAAGATTATCA TCCGGCAGCCTATCCGCACTGAGTACAAGATTGCTTTTCCTTACTTGTACAACAATCTTCCACACCATGT CCACCTCACCTGGTACCATACTCCCAATGTTGTATTCATCAAAACTGAGGATCCTGACTTGCCAGCTTTC TACTTTGACCCTTTGATCAACCCAATCTCCCATAGGCACTCAGTCAAGAGCCAGGAACCATTGCCGGATG ATGATGAGGAATTTGAGCTCCCGGAGTTTGTGGAGCCCTTCCTGAAGGACACACCCCTCTATACAGACAA TACAGCCAATGGCATTGCCCTGCTCTGGGCCCCGCGGCCCTTCAACCTACGCTCTGGTCGCACCCGTCGG GCCCTGGACATACCCCTTGTCAAGAACTGGTATCGGGAGCATTGTCCTGCCGGGCAGCCTGTGAAAGTGA GGGTCTCCTACCAGAAGCTGCTTAAGTACTATGTGCTGAATGCCCTGAAGCATCGGCCCCCTAAGGCTCA AAAGAAGAGGTATTTGTTCCGCTCCTTCAAAGCCACCAAATTCTTTCAGTCCACAAAGCTGGACTGGGTG GAGGTTGGGCTCCAGGTTTGCCGCCAGGGCTACAACATGCTCAACCTTCTCATTCACCGCAAAAACCTCA ACTACCTGCACCTGGACTACAACTTCAACCTCAAGCCTGTGAAAACGCTCACCACCAAGGAAAGAAAGAA ATCTCGTTTTGGGAATGCTTTCCACCTGTGTCGGGAAGTTCTGCGTTTGACTAAGCTGGTGGTGGATAGT CACGTGCAGTATCGGCTGGGCAATGTGGATGCCTTCCAGCTGGCAGATGGATTGCAGTATATATTTGCCC ATGTTGGGCAGTTGACGGGCATGTATCGATACAAATACAAGCTGATGCGACAGATTCGCATGTGCAAGGA CCTGAAGCATCTCATCTATTATCGTTTCAACACGGGCCCTGTAGGGAAGGGTCCTGGCTGTGGCTTCTGG GCTGCCGGTTGGCGAGTCTGGCTCTTTTTCATGCGTGGCATTACCCCTTTATTAGAGCGATGGCTTGGCA ACCTCCTGGCCCGGCAGTTTGAAGGTCGACACTCAAAGGGGGTGGCAAAGACAGTAACAAAGCAGCGAGT GGAGTCACATTTTGACCTTGAGCTGCGGGCAGCTGTGATGCATGATATTCTGGACATGATGCCTGAGGGG ATCAAGCAGAACAAGGCCCGGACAATCCTGCAGCACCTCAGTGAAGCCTGGCGCTGCTGGAAAGCCAACA TTCCCTGGAAGGTCCCTGGGCTGCCGACGCCCATAGAGAATATGATCCTTCGATACGTGAAGGCCAAGGC TGACTGGTGGACCAACACTGCCCACTACAACCGAGAACGGATCCGCCGAGGGGCCACTGTGGACAAGACT GTTTGTAAAAAGAATCTGGGCCGCCTCACCCGGCTCTATCTGAAGGCAGAACAGGAGCGGCAGCACAACT ACCTGAAGGACGGGCCTTACATCACAGCGGAGGAAGCAGTGGCAGTATATACCACCACAGTGCATTGGTT GGAAAGCCGCAGGTTTTCACCCATCCCATTCCCCCCACTCTCCTATAAGCATGACACCAAGTTGCTCATC TTGGCATTGGAGCGGCTCAAGGAAGCTTATAGTGTGAAGTCTCGGTTGAACCAGTCTCAGAGGGAGGAGC TAGGTCTGATCGAGCAGGCCTACGATAACCCCCACGAGGCGCTGTCCCGCATCAAGCGTCACCTCCTCAC ACAGAGAGCCTTCAAAGAGGTGGGCATTGAGTTCATGGATCTGTATAGCCACCTCGTTCCAGTATATGAT GTTGAGCCCCTGGAGAAGATAACTGATGCTTACCTGGACCAGTACCTGTGGTATGAAGCCGACAAGCGCC GCCTGTTCCCACCCTGGATTAAGCCTGCAGACACAGAACCACCTCCGCTGCTTGTTTACAAGTGGTGTCA AGGCATCAATAACCTGCAGGACGTGTGGGAGACGAGTGAAGGCGAGTGCAATGTCATGCTGGAATCCCGC TTTGAGAAGATGTATGAGAAGATCGACTTGACTCTGCTCAACAGGCTGCTGCGCCTCATCGTGGACCACA ACATAGCCGACTACATGACAGCCAAGAACAACGTCGTCATCAACTATAAGGACATGAACCATACGAATTC ATATGGGATCATCAGAGGCCTGCAGTTTGCCTCATTCATCGTGCAGTATTATGGCCTGGTGATGGATTTG CTTGTATTGGGATTGCACCGGGCCAGTGAGATGGCTGGGCCCCCTCAGATGCCAAATGACTTTCTCAGTT TCCAGGACATAGCCACTGAGGCTGCCCACCCCATCCGTCTCTTCTGCAGATACATTGATCGCATCCATAT TTTTTTCAGGTTCACAGCAGATGAGGCTCGGGACCTGATTCAACGTTACCTGACAGAGCACCCTGACCCC AATAATGAAAACATCGTTGGCTATAATAACAAGAAGTGCTGGCCCCGAGATGCCCGCATGCGCCTCATGA AACATGATGTTAACTTAGGCCGGGCGGTATTCTGGGACATCAAGAACCGCTTGCCACGGTCAGTGACTAC AGTTCAGTGGGAGAACAGCTTCGTGTCTGTGTACAGTAAGGACAACCCCAACCTGCTGTTCAACATGTGT GGCTTCGAGTGCCGCATCCTGCCTAAGTGCCGCACCAGCTATGAGGAGTTCACCCACAAGGACGGGGTCT GGAACCTGCAGAATGAGGTTACTAAGGAGCGCACAGCTCAGTGTTTCCTGCGTGTGGACGATGAGTCAAT GCAGCGCTTCCACAACCGCGTGCGTCAGATTCTCATGGCCTCTGGGTCCACCACCTTCACCAAGATTGTG AATAAGTGGAATACAGCTCTCATTGGCCTTATGACATACTTTCGGGAGGCTGTGGTGAACACCCAAGAGC TCTTGGACTTACTGGTGAAGTGTGAGAACAAAATCCAGACACGTATCAAGATTGGACTCAACTCCAAGAT GCCAAGTCGGTTCCCCCCGGTTGTGTTCTACACCCCTAAGGAGTTGGGTGGACTCGGCATGCTCTCAATG GGCCATGTGCTCATCCCCCAATCCGACCTCAGGTGGTCCAAACAGACAGATGTAGGTATCACACACTTTC GTTCAGGAATGAGCCATGAAGAAGACCAGCTCATTCCCAACTTGTACCGCTACATACAGCCATGGGAGAG CGAGTTCATTGATTCTCAGCGGGTCTGGGCTGAGTACGCACTCAAGAGGCAAGAGGCCATTGCTCAGAAC AGACGCCTGACTTTAGAAGACCTAGAAGATTCATGGGATCGTGGCATTCCTCGAATCAATACCCTCTTCC AGAAGGACCGGCACACACTGGCTTATGATAAGGGCTGGCGTGTCAGAACTGACTTTAAGCAGTATCAGGT TTTGAAGCAGAATCCGTTCTGGTGGACACACCAGCGGCATGATGGGAAGCTCTGGAACCTGAACAACTAC CGTACAGACATGATCCAGGCCCTGGGCGGTGTGGAAGGCATTCTGGAACACACACTCTTTAAGGGCACTT ACTTCCCTACCTGGGAGGGGCTTTTCTGGGAGAAGGCCAGTGGCTTTGAGGAATCTATGAAGTGGAAGAA GCTAACTAATGCTCAGCGATCAGGACTGAACCAGATTCCCAATCGTAGATTCACCCTCTGGTGGTCCCCG ACCATTAATCGAGCCAATGTATATGTAGGCTTTCAGGTGCAGCTAGACCTGACGGGTATCTTCATGCACG GCAAGATCCCCACGCTGAAGATCTCTCTCATCCAGATCTTCCGAGCTCACTTGTGGCAGAAGATCCATGA GAGCATTGTTATGGACTTATGTCAGGTGTTTGACCAGGAACTTGATGCACTGGAAATTGAGACAGTACAA AAGGAGACAATCCATCCCCGAAAGTCATATAAGATGAACTCTTCCTGTGCAGATATCCTGCTCTTTGCCT CCTATAAGTGGAATGTCTCCCGGCCCTCATTGCTGGCTGACTCCAAGGATGTGATGGACAGCACCACCAC CCAGAAATACTGGATTGACATCCAGTTGCGCTGGGGGGACTATGATTCCCACGACATTGAGCGCTACGCC CGGGCCAAGTTCCTGGACTACACCACCGACAACATGAGTATCTACCCTTCGCCCACAGGTGTACTCATCG CCATTGACCTGGCCTATAACTTGCACAGTGCCTATGGAAACTGGTTCCCAGGCAGCAAGCCTCTCATACA ACAGGCCATGGCCAAGATCATGAAGGCAAACCCTGCCCTGTATGTGTTACGTGAACGGATCCGCAAGGGG CTACAGCTCTATTCATCTGAACCCACTGAGCCTTATTTGTCTTCTCAGAACTATGGTGAGCTCTTCTCCA ACCAGATTATCTGGTTTGTGGATGACACCAACGTCTACAGAGTGACTATTCACAAGACCTTTGAAGGGAA CTTGACAACCAAGCCCATCAACGGAGCCATCTTCATCTTCAACCCACGCACAGGGCAGCTGTTCCTCAAG ATAATCCACACGTCCGTGTGGGCGGGACAGAAGCGTTTGGGGCAGTTGGCTAAGTGGAAGACAGCTGAGG AGGTGGCCGCCCTGATCCGATCTCTGCCTGTGGAGGAGCAGCCCAAGCAGATCATTGTCACCAGGAAGGG CATGCTGGACCCACTGGAGGTGCACTTACTGGACTTCCCCAATATTGTCATCAAAGGATCGGAGCTCCAA CTCCCTTTCCAGGCGTGTCTCAAGGTGGAAAAATTCGGGGATCTCATCCTTAAAGCCACTGAGCCCCAGA TGGTTCTCTTCAACCTCTATGACGACTGGCTCAAGACTATTTCATCTTACACGGCCTTCTCCCGTCTCAT CCTGATTCTGCGTGCCCTACATGTGAACAACGATCGGGCAAAAGTGATCCTGAAGCCAGACAAGACTACT ATTACAGAACCACACCACATCTGGCCCACTCTGACTGACGAAGAATGGATCAAGGTCGAGGTGCAGCTCA AGGATCTGATCTTGGCTGACTACGGCAAGAAAAACAATGTGAACGTGGCATCACTGACACAATCAGAAAT TCGAGACATCATCCTGGGTATGGAGATCTCGGCACCGTCACAGCAGCGGCAGCAGATCGCTGAGATCGAG AAGCAGACCAAGGAACAATCGCAGCTGACGGCAACACAGACTCGCACTGTCAACAAGCATGGCGATGAGA TCATCACCTCCACCACCAGCAACTATGAGACCCAGACTTTCTCATCCAAGACTGAGTGGAGGGTCAGGGC CATCTCTGCTGCCAACCTGCACCTAAGGACCAATCACATCTATGTTTCATCTGACGACATCAAGGAGACT GGCTACACCTACATCCTTCCCAAGAATGTGCTTAAGAAGTTCATCTGCATATCTGACCTTCGGGCCCAAA TTGCAGGATACCTATATGGGGTGAGCCCACCAGATAACCCCCAGGTGAAGGAGATCCGCTGCATTGTGAT GGTGCCGCAGTGGGGCACTCACCAGACCGTGCACCTGCCTGGCCAGCTGCCCCAGCATGAGTACCTCAAG GAGATGGAACCCTTAGGTTGGATCCACACTCAGCCCAATGAGTCCCCGCAGTTATCACCCCAGGATGTCA CCACCCATGCCAAGATCATGGCTGACAACCCATCTTGGGATGGCGAGAAGACCATTATCATCACATGCAG CTTCACGCCAGGCTCCTGTACACTGACGGCCTACAAGCTGACCCCCAGTGGCTACGAATGGGGCCGCCAG AACACAGACAAGGGCAACAACCCCAAGGGCTACCTGCCTTCACACTATGAGAGGGTGCAGATGCTGCTGT CGGACCGTTTCCTTGGCTTCTTCATGGTCCCTGCCCAGTCCTCGTGGAACTACAACTTCATGGGTGTTCG GCATGACCCCAACATGAAATATGAGCTACAGCTGGCGAACCCCAAAGAGTTCTACCACGAGGTGCACAGG CCCTCTCACTTCCTCAACTTTGCTCTCCTGCAGGAGGGGGAGGTTTACTCTGCGGATCGGGAGGACCTGT ATGCCTGA >OriGene 5' read for NM_006445 unedited
NGTTAGCATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGCGGACGCAG TCTGGCCGCCATTGCGCTGCGGGGAAAGCGGCCTCTTGTGTGAGGGCCTGTGGGATTCTC CGGATATGGCCGGAGTGTTTCCTTATCGAGGGCCGGGTAACCCGGTGCCTGGCCCTCTAG CCCCGCTACCGGACTACATGTCGGAGGAGAAGCTGCAGGAGAAAGCTCGAAAATGGCAGC AATTGCAGGCCAAGCGCTATGCAGAAAAGCGGAAGTTTGGGTTTGTGGATGCCCAGAAGG AAGACATGCCCCCAGAACATGTCAGGAAGATCATTCGAGACCATGGAGACATGACCAACA GGAAGTTCCGCCATGACAAAAGGGTTTACTTGGGTGCCCTAAAGTACATGCCCCACGCAG TCCTCAAACTCCTGGAGAACATGCCTATGCCTTGGGAGCAGATTCGGGATGTGCCTGTGC TGTACCACATCACTGGAGCCATTTCCTTCGTCAATGAGATTCCCTGNGTCATTGAACCTG TCTACATCTCCCAGTGGGGGTCAATGTGGATTATGATGCGCCGAGAAAAAAGAGATAGGA GGCATTTCAAGAGGATGCGTTTTCCCCCTTTTGATGATGAGGAGCCGCCCTTGGACTATG CTGANCACATCCTAGATGTTGAGCCACTGGAGGCCATTCAGCTAGAGCTGGACCCTNGAG AGGACGCCCCTGTGTTGGACTGGTTCTATGACCACCAGCCGTTGAGGGNACAGCAGAAGT ATGTAAATGGCTCCACTTACCAGCGCTGGCAGTTCACACTACCTATGATGTCGACTCTCT ACCGNCTGGNCTATCAGCTNCTGACAGACTGGNTGGATGACAACTACTNCTACCTGTTNN GATT >OriGene 3' read for NM_006445 unedited
CTATGAACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTCCTATTTATACAAAATTTATTATTATATTTTATTCAGGATGACAA CCCATCAGGAGGTCAACAACCCAACCCCAGACAGAGGGAAAAAGGCCAAACTGCTGAATG TCACCGGCCTGTCTGGAGGGGCTGAGGCTTCGGCCTCGGGAGGCTGAACCAGGAGGCAGG GAAACGGTCAGGCATACAGGTCCTCCCGATCCGCAAAGTAAACCTCCCCCTCCTGCAGGA AACCAAATTTGAGGAAGTGAAAGGGCCTGTCCACCTCGTGGAAAAACTCTTTGGGGTTCC CCAGCTGTACCTCATATTTCATGTTGGGGTCATGCCAAACACCCATGAAGTTGTATTTCC ACGAGGACTGGGCAGGGACCATGAAAAACCCAAGGAAACGGTCCGACAGCAGCATCTGCA CCCTTTAATAGTGTGAAGGCAGGTACCCCTTGGGGTTGTTGCCCTTGTCTGTGTTCTGGC GGCCCCATTCGTAGCCACTGGGGGTCACCTTGTAGGCCGTCAGTGTACAGGACCCTGGCG TGAACCTGCATGTGATGATAATGGTCTTCTCGCCATCCCAAGATGGGTTGCCACCCATGA TCTTGGCATGGGTGGTGACATCCTGGGGTGATAACTGCGGGGACTCATTGGGCTGATTGT GGATCCAACCTCAGGGTTCCATCTTCCTGAAGAACTCATGCTGGGGCACCTGCCCAGCAA GTGCCCGTCTTGGAAGTGCCCCATGGGCACCTTACATGCAAGGGATCTCCTTACCTGGGG GTATTGGGGGGCCCCCCCCATATAGTTCCCGCATTTGGCCCAAGCACAATTCCAATT |
Restriction Sites | NotI-NotI |
ACCN | NM_006445 |
Insert Size | 7140 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_006445.2, NP_006436.2 |
RefSeq Size | 7311 bp |
RefSeq ORF | 7311 bp |
Locus ID | 10594 |
UniProt ID | Q6P2Q9 |
Cytogenetics | 17p13.3 |
Domains | JAB_MPN |
Protein Families | Druggable Genome |
Protein Pathways | Spliceosome |
Gene Summary | Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq, Jul 2008] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209472 | PRPF8 (Myc-DDK-tagged)-Human PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae) (PRPF8) |
USD 1,927.00 |
|
RC209472L1 | Lenti ORF clone of Human PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae) (PRPF8), Myc-DDK-tagged |
USD 2,227.00 |
|
RG209472 | PRPF8 (tGFP-tagged) - Human PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae) (PRPF8) |
USD 2,127.00 |
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