GNAI3 (NM_006496) Human Untagged Clone

SKU SC116066

GNAI3 (untagged)-Human guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 (GNAI3)

Product Data

• Type: Human Untagged Clone
• Target Symbol: GNAI3
• Synonyms: 87U6; ARCND1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006496, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGGCTGCACGTTGAGCGCCGAAGACAAGGCGGCAGTGGAGCGAAGCAAGATGATCGACCGCAACTTAC; GGGAGGACGGGGAAAAAGCGGCCAAAGAAGTGAAGCTGCTGCTACTCGGTGCTGGAGAATCTGGTAAAAG; CACCATTGTGAAACAGATGAAAATCATTCATGAGGATGGCTATTCAGAGGATGAATGTAAACAATATAAA; GTAGTTGTCTACAGCAATACTATACAGTCCATCATTGCAATCATAAGAGCCATGGGACGGCTAAAGATTG; ACTTTGGGGAAGCTGCCAGGGCAGATGATGCCCGGCAATTATTTGTTTTAGCTGGCAGTGCTGAAGAAGG; AGTCATGACTCCAGAACTAGCAGGAGTGATTAAACGGTTATGGCGAGATGGTGGGGTACAAGCTTGCTTC; AGCAGATCCAGGGAATATCAGCTCAATGATTCTGCTTCATATTATCTAAATGATCTGGATAGAATATCCC; AGTCTAACTACATTCCAACTCAGCAAGATGTTCTTCGGACGAGAGTGAAGACCACAGGCATTGTAGAAAC; ACATTTCACCTTCAAAGACCTATACTTCAAGATGTTTGATGTAGGTGGCCAAAGATCAGAACGAAAAAAG; TGGATTCACTGTTTTGAGGGAGTGACAGCAATTATCTTCTGTGTGGCCCTCAGTGATTATGACCTTGTTC; TGGCTGAGGACGAGGAGATGAACCGAATGCATGAAAGCATGAAACTGTTTGACAGCATTTGTAATAACAA; ATGGTTTACAGAAACTTCAATCATTCTCTTCCTTAACAAGAAAGACCTTTTTGAGGAAAAAATAAAGAGG; AGTCCGTTAACTATCTGTTATCCAGAATACACAGGTTCCAATACATATGAAGAGGCAGCTGCCTATATTC; AATGCCAGTTTGAAGATCTGAACAGAAGAAAAGATACCAAGGAGATCTATACTCACTTCACCTGTGCCAC; AGACACGAAGAATGTGCAGTTTGTTTTTGATGCTGTTACAGATGTCATCATTAAAAACAACTTAAAGGAA; TGTGGACTTTATTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006496 unedited; GCACGAGGCCGAGCCGCAGTTTCCGTGGTGTGAGTGAGTCCGGGCCCGTGTCCCCTCTCC; CGCCGCCGCCATGGGCTGCACGTTGAGCGCCGAAGACAAGGCGGCAGTGGAGCGAAGCAA; GATGATCGACCGCAACTTACGGGAGGACGGGGAAAAAGCGGCCAAAGAAGTGAAGCTGCT; GCTACTCGGTGCTGGAGAATCTGGTAAAAGCACCATTGTGAAACAGATGAAAATCATTCA; TGAGGATGGCTATTCAGAGGATGAATGTAAACAATATAAAGTAGTTGTCTACAGCAATAC; TATACAGTCCATCATTGCAATCATAAGAGCCATGGGACGGCTAAAGATTGACTTTGGGGA; AGCTGCCAGGGCAGATGATGCCCGGCAATTATTTGTTTTAGCTGGCAGTGCTGAAGAAGG; AGTCATGACTCCAGAACTAGCAGGAGTGATTAAACGGTTATGGCGAGATGGTGGGGTACA; AGCTTGCTTCAGCAGATCCAGGGAATATCAGCTCAATGATTCTGCTTCATATTATCTAAA; TGATCTGGATAGAATATCCCAGTCTAACTACATTCCAACTCAGCAAGATGTTCTTCGGAC; GAGAGTGAAGACCACAGGCATTGTAGAAACACATTTCACCTTCA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006496 unedited; AAGCGCCGCGCGCCATCTACAGTAGAGGAGTTTTTTTTTTTTTTTGAAATTCATAATTTT; ATTGAAATGAGCTGTCTAACATAAATTAATCTGTGATTTTTATGTACCATTATAGTACTG; ACAACAACTGTAACAACATTAAAAATGTTCACATGGACACAGTGAAGATGAGTTCTAAAG; ATGATACAGTTTGAGCAAAAGAACATACTGGCTAAGGATTACTACAGTTAACATAAGGGA; CAGTAAAAACGATGGCAAACAGGGATTTGATACCACATTTACAAAGTAAAAGCATGCACT; GTTAATACACTTTAAATGTTTCTCAACAGAAAAGGCCATGAAGATGGAAAACAAGAGGCA; CCATGTACAAAACTCCCTATAACTGAGACAAACAAGCAAGAATCAAAGTGGTCAATTTAG; TAAATATGTAGCAGCAAAGTCACTGGTTCTGTTTGGAATTTAGCAATTTGCATTTCTGAT; TGGCAGCTGCCCTGGGTGTGTCTGTATCCAAGAAGCTGACTTTATCATACTACATCAGCA; GTAATTTGGTAATCTGCACAAACAAGGTTAACCTTCAACCATAAGCTTAAAGAAAAGAGA; GAACTAGAATCTTATTGCAGAACTTTCCAATGNTATTACCATATGGAAACCAATATGGTA; CTTTGAGCAGGATAATAACATAAATTTCATTTAAAAAGTTGTATTTATAGCCCCAGTAAC; CGGAAAAGATTATAAGTAATTATGGAAAGTTTATATTCTGACCATACCACGAGTTAAAAA; CAAAGAGTTCCTACTAAAGAGAATATTTTCAAGAAGATCTTGTCACATCATGTGCATAGT; AAGATAGGTTGTTTTAATAAAATCCTTTGCAATAAAGAAATAAAATTATTAAAGTATTCT; TTCTTGAGGNCACNCCTAAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_006496
• Insert Size: 2660 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006496.1, NP_006487.1
• RefSeq Size: 3143 bp
• RefSeq ORF: 1065 bp
• Locus ID: 2773
• UniProt ID: P08754
• Cytogenetics: 1p13.3
• Domains: G-alpha
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance, Chemokine signaling pathway, Gap junction, Leukocyte transendothelial migration, Long-term depression, Melanogenesis, Progesterone-mediated oocyte maturation, Tight junction
• Summary: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway. [provided by RefSeq, Jun 2012]