Fibulin 1 (FBLN1) (NM_006486) Human Untagged Clone

CAT#: SC116062

FBLN1 (untagged)-Human fibulin 1 (FBLN1), transcript variant D

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Fibulin 1
• Synonyms: FBLN; FIBL1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116062 sequence for NM_006486 edited (data generated by NextGen Sequencing)
  ATGGAGCGCGCCGCGCCGTCGCGCCGGGTCCCGCTTCCGCTGCTGCTGCTCGGCGGCCTT
  GCGCTGCTGGCGGCCGGAGTGGACGCGGATGTCCTCCTGGAGGCCTGCTGTGCGGACGGA
  CACCGGATGGCCACTCATCAGAAGGACTGCTCGCTGCCATATGCTACGGAATCCAAAGAA
  TGCAGGATGGTGCAGGAGCAGTGCTGCCACAGCCAGCTGGAGGAGCTGCACTGTGCCACG
  GGCATCAGCCTGGCCAACGAGCAGGACCGCTGTGCCACGCCCCACGGTGACAACGCCAGC
  CTGGAGGCCACATTTGTGAAGAGGTGCTGCCATTGCTGTCTGCTGGGGAGGGCGGCCCAG
  GCCCAGGGCCAGAGCTGCGAGTACAGCCTCATGGTTGGCTACCAGTGTGGACAGGTCTTC
  CGGGCATGCTGTGTCAAGAGCCAGGAGACCGGAGATTTGGATGTCGGGGGCCTCCAAGAA
  ACGGATAAGATCATTGAGGTTGAGGAGGAACAAGAGGACCCATATCTGAATGACCGCTGC
  CGAGGAGGCGGGCCCTGCAAGCAGCAGTGCCGAGACACGGGTGACGAGGTGGTCTGCTCC
  TGCTTCGTGGGCTACCAGCTGCTGTCTGATGGTGTCTCCTGTGAAGATGTCAATGAATGC
  ATCACGGGCAGCCACAGCTGCCGGCTTGGAGAATCCTGCATCAACACAGTGGGCTCTTTC
  CGCTGCCAGCGGGACAGCAGCTGCGGGACTGGCTATGAGCTCACAGAGGACAATAGCTGC
  AAAGATATTGACGAGTGTGAGAGTGGTATTCATAACTGCCTCCCCGATTTTATCTGTCAG
  AATACTCTGGGATCCTTCCGCTGCCGACCCAAGCTACAGTGCAAGAGTGGCTTTATACAA
  GATGCTCTAGGCAACTGTATTGATATCAATGAGTGTTTGAGTATCAGTGCCCCGTGCCCT
  ATYGGGCATACATGCATCAACACAGAGGGCTCCTACACGTGCCAGAAGAACGTGCCCAAC
  TGTGGCCGTGGCTACCATCTCAACGAGGAGGGAACGCGCTGTGTTGATGTGGACGAGTGC
  GCGCCACCTGCTGAGCCCTGTGGGAAGGGACATCGCTGCGTGAACTCTCCCGGCAGTTTC
  CGCTGCGAATGCAAGACGGGTTACTATTTTGACGGCATCAGCAGGATGTGTGTCGATGTC
  AACGAGTGCCAGCGCTACCCCGGGCGCCTGTGTGGCCACAAGTGCGAGAACACGCTGGGC
  TCCTACCTCTGCAGCTGTTCCGTGGGCTTCCGGCTCTCTGTGGATGGCAGGTCATGTGAA
  GACATCAATGAGTGCAGCAGCAGCCCCTGTAGCCAGGAGTGTGCCAACGTCTACGGCTCC
  TACCAGTGTTACTGCCGGCGAGGCTACCAGCTCAGCGATGTGGATGGAGTCACCTGTGAA
  GACATCGACGAGTGCGCCCTGCCCACCGGGGGCCACATCTGCTCCTACCGCTGCATCAAC
  ATCCCTGGAAGCTTCCAGTGCAGCTGCCCCTCGTCTGGCTACAGGCTGGCCCCCAATGGC
  CGCAACTGCCAAGACATTGATGAGTGTGTGACTGGCATCCACAACTGCTCCATCAACGAG
  ACCTGCTTCAACATCCAGGGCGGCTTCCGCTGCCTGGCCTTCGAGTGCCCTGAGAACTAC
  CGCCGCTCCGCAGCCACGCTCCAGCAGGAGAAGACAGACACGGTCCGCTGCATCAAGTCC
  TGCCGCCCCAACGATGTCACATGCGTGTTCGACCCCGTGCACACCATCTCCCACACCGTC
  ATCTCGCTGCCTACCTTCCGCGAGTTCACCCGCCCTGAAGAGATCATCTTCCTCCGGGCC
  ATCACGCCACCGCATCCTGCCAGCCAGGCTAACATCATCTTCGACATCACGGAAGGGAAC
  CTGCGGGACTCTTTTGACATCATCAAGCGTTACATGGACGGCATGACCGTGGGTGTCGTG
  CGCCAGGTGCGGCCCATCGTGGGCCCATTTCATGCCGTCCTGAAGCTGGAGATGAACTAT
  GTGGTCGGGGGCGTGGTCTCCCACCGAAATGTTGTCAACGTCCACATCTTCGTCTCTGAG
  TACTGGTTCTGA
  
  Clone variation with respect to NM_006486.2
  963 c=>y
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006486 unedited
  NGGAAGCCGTTTGNGATATTTGTATACGACTTACTATAGGGNNCGGCCGCGCAATTCGGC
  ACGAGGGCGGCCCTGGCCCAGCGTTGGCTGCCGAGGCTCGGCCGGAGCGTGGAGCCCGCG
  CCGCTGCCCCAGGACCGCGCCCGCGCCTTTGTCCGCCGCCGCCCACCGCCCGTCGCCCGC
  CGCCCATGGAGCGCGCCGCGCCGTCGCGCCGGGTCCCGCTTCCGCTGCTGCTGCTCGGCG
  GCCTTGCGCTGCTGGCGGCCGGAGTGGACGCGGATGTCCTCCTGGAGGCCTGCTGTGCGG
  ACGGACACCGGATGGCCACTCATCAGAAGGACTGCTCGCTGCCATATGCTACGGAATCCA
  AAGAATGCAGGATGGTGCAGGAGCAGTGCTGCCACAGCCAGCTGGAGGAGCTGCACTGTG
  CCACGGGCATCAGCCTGGCCAACGAGCAGGACCGCTGTGCCACGCCCCACGGTGACAACG
  CCAGCCTGGAGGCCACATTTGTGAAGAGGTGCTGCCATTGCTGTCTGCTGGGGAGGGCGG
  CCCAGGCCCAGGGCCAGAGCTGCGAGTACAGCCTCATGGTTGGCTACCAGTGTGGACAGG
  TCTTCCGGGCATGCTGTGTCAAGAGCCAGGAGACCGGAGATTTGGATGTCGGGGGCCTCC
  AAGAAACGGATAAGATCATTGAGGTTGAGGAGGAACAAGAGGACCCATATCTGAATGACC
  GCTGCCGAGGGAGCGGGCCCTGCAAGCAGCAGTGCCGAGACACGGGTGAACGAGGTGGTC
  TGCTCCTGCTTCGTGGGCTACCAGCTGCTGTCTGATGGTGTCTCCTGTGGAGATGTCAAT
  GAATGCATCACGGGCAGCCACAGCTGCCGGCTTGGGAGAATCTGCATC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006486 unedited
  GCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTAAGGAGGATCACAAAGT
  TGTTTATTTTCCCACCTATAAAAAAAGAGGATGATGCAAAGTGCCGAAGAAATCCCAGCC
  ATTCCCCTGGTGAACATACTGGAGGCCCACACGAGCCTAACAGGCTGTGTCTTTACCAAG
  GGATTTTCCTCTATTTCAACAGAACTGCAATTTCCCTTCCAAGTGCTCCCCGAGGCATTC
  TGTCATGCTGCTTCTGGGGATAATTTATCATCCACGTCTGTGCTATTCTACAGCAGCCAT
  GTCCTTAAAACATAAAAAATCTGATTGGCCAAAAAAAAAAAAAAGTGCCAAAATGTCAAA
  TTTCATACAATCAGGGCAAGAACTGGTCTTCTTTTGGCATTTGGACTGGCTGCCCCCTAA
  AAAATGTCTTTCTTTCCTTGGCCCTTATCAAGGCCCAGCCAGAGGCAGAGAAAGGGGGTG
  ACACTTACCCTTCGCATTAAAAAATGAGCAAGGTTTTCAACCAAACAGTTGAGCAACTGG
  GCTCATTTTTTAAACACCGAAAATACATCAGATGGACTTATTCATCTGGCTCAGCTTAAT
  ACATGTTGGCCTAATGCTACAAATACCTAACATTAAAAAAGTCTGAGGGTATAAACAAGT
  ACAGACCACAGTCACTGGCAGCAATGATTGGGCCTGGAGGTGCACCTGCGGCTGTGCGAA
  AACCAGCCCTCAAACCAGTCCTCAGAGACCAAGATGTGGACGTTGACAACTTTTCGGAGG
  GAGACCACGCCCCCGACCACTATTTATTCTCCACTTCAGACAGCATGAAAGGGCCACTAT
  GGCCCACCTGGCCACAAACCACGTTATCCGGCCTGTAACCTTGTAAGTCAAAGAGCCCCA
  GCTCCCTCCGGATCAAATAAATTACCCTCCTGCAGATCCGGCCCTAGCCCCAAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006486
• Insert Size: 3160 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006486.2, NP_006477.2
• RefSeq Size: 2947 bp
• RefSeq ORF: 2112 bp
• Locus ID: 2192
• UniProt ID: P23142
• Cytogenetics: 22q13.31
• Domains: ANATO, EGF_CA, EGF, EGF
• Protein Families: Secreted Protein
• Gene Summary: Fibulin 1 is a secreted glycoprotein that becomes incorporated into a fibrillar extracellular matrix. Calcium-binding is apparently required to mediate its binding to laminin and nidogen. It mediates platelet adhesion via binding fibrinogen. Four splice variants which differ in the 3' end have been identified. Each variant encodes a different isoform, but no functional distinctions have been identified among the four variants. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (D) encodes the longest isoform (D).