RGS14 (NM_006480) Human Untagged Clone

SKU SC116059

RGS14 (untagged)-Human regulator of G-protein signaling 14 (RGS14)

Product Data

• Type: Human Untagged Clone
• Target Symbol: RGS14
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116059 sequence for NM_006480 edited (data generated by NextGen Sequencing); ATGCCAGGGAAGCCCAAGCACCTGGGCGTCCCCAACGGGCGCATGGTTCTGGCTGTGTCA; GATGGAGAGCTGAGCAGCACGACGGGGCCCCAGGGCCAGGGCGAGGGCCGCGGCAGCTCT; CTCAGCATCCACAGCCTCCCCAGTGGTCCCAGCAGCCCCTTCCCAACCGAGGAGCAGCCT; GTGGCCAGCTGGGCCCTGTCCTTCGAGCGGCTGTTGCAGGACCCGCTGGGCCTGGCTTAC; TTCACTGAGTTCCTGAAGAAGGAGTTCAGCGCGGAAAACGTGACTTTCTGGAAGGCCTGC; GAGCGCTTCCAGCAGATCCCGGCCAGCGATACCCAGCAGCTAGCTCAGGAGGCCCGCAAC; ATCTACCAGGAGTTCCTGTCCAGCCAGGCGCTGAGCCCAGTGAACATCGACCGTCAGGCC; TGGCTTGGCGAGGAGGTGCTGGCCGAGCCCCGGCCGGACATGTTTCGGGCACAGCAGCTT; CAGATCTTCAACTTGATGAAGTTCGACAGCTATGCGCGCTTCGTCAAGTCCCCGCTGTAC; CGCGAGTGCCTGCTAGCCGAAGCCGAGGGACGCCCTCTGCGGGAACCTGGCTCCTCGCGC; CTCGGCAGCCCTGACGCCACGAGGAAGAAGCCGAAGCTGAAGCCCGGGAAGTCGCTGCCG; CTGGGTGTGGAGGAGTTGGGGCAGCTGCCACCCGTTGAGGGTCCTGGGGGCCGCCCTCTC; CGCAAGTCCTTCCGCCGGGAGCTGGGCGGGACTGCAAACGCCGCCTTGCGCCGAGAGTCT; CAGGGCTCCCTCAACTCCTCCGCCAGCCTGGACCTTGGCTTCCTAGCCTTCGTCAGCAGC; AAATCTGAGAGCCACCGGAAGAGCCTTGGGAGCACGGAGGGTGAAAGTGAAAGCCGGCCA; GGGAAGTACTGCTGTGTGTACCTGCCCGATGGCACAGCCTCCTTGGCCCTGGCCAGACCT; GGCCTCACCATCCGAGACATGCTGGCAGGGATCTGTGAGAAACGAGGCCTCTCTCTACCT; GACATCAAGGTCTACCTGGTGGGCAATGAACAGGCCCTGGTCCTGGATCAGGACTGCACC; GTGCTGGCGGATCAGGAAGTGCGGCTGGAAAACAGGATCACCTTCGAGCTGGAGCTGACG; GCGCTGGAGCGCGTGGTACGAATCTCAGCCAAGCCCACCAAGCGGCTGCAGGAGGCGCTG; CAGCCCATTCTGGAGAAGCACGGCTTGAGCCCGCTAGAGGTGGTGCTGCACCGGCCAGGC; GAGAAACAGCCTCTGGATCTGGGGAAGCTAGTGAGCTCGGTGGCGGCCCAGAGACTGGTT; TTGGACACTCTTCCAGGTGTGAAGATCTCCAAAGCCCGTGACAAATCTCCCTGCCGCAGC; CAGGGCTGCCCACCTAGAACTCAGGATAAGGCCACCCATCCCCCTCCAGCGTCCCCCAGT; TCTCTGGTGAAGGTGCCCAGTAGTGCCACTGGAAAGCGGCAGACCTGTGACATCGAAGGC; CTGGTGGAGCTGCTGAACCGGGTGCAGAGCAGCGGGGCCCACGACCAGAGGGGCCTTCTG; AGGAAAGAGGACCTGGTACTTCCAGAATTTCTGCAGCTGCCCGCCCAAGGGCCCAGCTCC; GAGGAGACCCCACCACAGACCAAATCAGCAGCCCAGCCCATCGGGGGATCCTTGAACTCC; ACCACCGACTCAGCCCTCTGA; ; Clone variation with respect to NM_006480.4;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006480 unedited; CAAATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACCAGGCCGAGTCAG; GCTCTCTCCCTGCCTGCCCGCCACCGTGCAAGCTCTGGCCGGCGCTGCCCACAGTCCCCA; TGGTGGGCAGCCCCCGCGGCGGGGACCCCTGATCGGCAGCGGCATGCCAGGGAAGCCCAA; GCACCTGGGCGTCCCCAACGGGCGCATGGTTCTGGCTGTGTCAGATGGAGAGCTGAGCAG; CACGACGGGGCCCCAGGGCCAGGGCGAGGGCCGCGGCAGCTCTCTCAGCATCCACAGCCT; CCCCAGTGGTCCCAGCAGCCCCTTCCCAACCGAGGAGCAGCCTGTGGCCAGCTGGGCCCT; GTCCTTCGAGCGGCTGTTGCAGGACCCGCTGGGCCTGGCTTACTTCACTGAGTTCCTGAA; GAAGGAGTTCAGCGCGGAAAACGTGACTTTCTGGAAGGCCTGCGAGCGCTTCCAGCAGAT; CCCGGCCAGCGATACCCAGCAGCTAGCTCAGGAGGCCCGCAACATCTACCAGGAGTTCCT; GTCCAGCCAGGCGCTGAGCCCAGTGAACATCGACCGTCAGGCCTGGCTTGGCGAGGAGGT; GCTGGCCGAGCCCCCGCCGGACATGTTTCGGGCACAGCAGCTTCAGATCTTCAACTTGAT; GAAGTTCGACAGCTATGCGCGCTTCGTCAAGTCCCCGCTGTACCGCGAGTGCCTGCTAGC; CGAAGCCGAGGGACGCCCTCTGCGGNAACCTGGNCTCTCGCGCCCTCGCAGCCCTGACGC; CACGAGGAAGAAGCCGAAGCTGAAGCCCNGGAAGTCGCTGCCGCTGGGTGTGGAGGAAGN; TGGNGCANCTGCCANCCGNNTGAGGGTCCTGGGGGGCCGNCCCTCTTCGCAGTCCTTTNC; GCGGNAGCCTGGCGGAACTGCAAACGCCCGCTGCGCCGANAGTCTAAGGNCTCCTTCAAC; TCTNCGCAGCCTGGACCTTGTTTNCTAGNCTCGTAGCAGAAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006480 unedited; GACGCCGGACAAACCCCACCCTTTTNNTTANTTTTACTTGNACCCGGCCGCATACTANGA; TCGATTTTTTTTTTTTTTTTTTTTGCAGAAAAATAATAATTATTATAATAACAGTATCAT; ACATCAAGGACAAACCCAGCTGCCCCAAGCCTACTGCAAAATCTGTATGTACAGTATAGT; CTATGTGGGTGGGGTACAGGGCTGCCTGCCTGCACCCTCAAGGCCTTACTCATACCAGCT; TCCTGAGGAGGGGCCGGCCCCTCCTCTTGCCCCTGTTGAAGCTTGGCACAGGCTGGGGAG; GCTGGCACTGCCAACGCCATCCCTCCATGTTGGGCAAGCCTGTTCCAAGGGGCTGGACTC; ACCTCCCCCATTGTGGCCTGGCTGCAAGGGATTGGGGGTGAGCTTGTTGAGGGACAAGGC; GGTGGCAGCTGTGGGGTGTGTCTCATCTGAGTCCCCTTTCCACCCCTACCGGCTCTTCCT; GCGGGCCTGCCCATGGCAGGAAGGGGCCAGGGACACTCATGGCACAGACAGGGCATGCAG; AGCGGACCCATGGCATGCTCGGCCCGCCGGGTGCCATGCAGCTGTCCTGGACTGTTGGGT; AGCTGTCAAAGGGCTGAGTCGGTGGTGGAGTTCAAGGATCCCCCGATGGGCTGGGCTGCT; GATTTGGTCTGTGGTGGGGTCTTCTCTGGACCTGGCCCTTGGGCGGGCAGTTGCAAAAAT; CTTGAAAGACCCAGTCCTTTTTCCTTAAAAGGCCCTTTTGTCCGGGGGCCCCCTGGTCTT; GAACCTGTTTAAGAACTTTCCCCAGCTTTTTATGTCACAAGTTTTGCCCCTTTCCAAGGG; GACTACGGGGCACCTCCCCACAAAATGGGGGACCCTTTAGGGGCAAGGGCGGCCTTATCT; CTGATTTTAGGGGGCAAACCCTGCTTCGCAAGAAAATTTTTACCGGTTTCTAAATTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_006480
• Insert Size: 2560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006480.4, NP_006471.2
• RefSeq Size: 2418 bp
• RefSeq ORF: 1701 bp
• Locus ID: 10636
• UniProt ID: O43566
• Cytogenetics: 5q35.3
• Domains: GoLoco, RBD, RGS
• Protein Families: Druggable Genome
• Summary: This gene encodes a member of the regulator of G-protein signaling family. This protein contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]