G protein alpha 13 (GNA13) (NM_006572) Human Untagged Clone

SKU SC116002

GNA13 (untagged)-Human guanine nucleotide binding protein (G protein), alpha 13 (GNA13)

Product Data

• Type: Human Untagged Clone
• Target Symbol: G protein alpha 13
• Synonyms: G13
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116002 sequence for NM_006572 edited (data generated by NextGen Sequencing); ATGGCGGACTTCCTGCCGTCGCGGTCCGTGCTGTCCGTGTGCTTCCCCGGCTGCCTGCTG; ACGAGTGGCGAGGCCGAGCAGCAACGCAAGTCCAAGGAGATCGACAAATGCCTGTCTCGG; GAAAAGACCTATGTGAAGCGGCTGGTGAAGATCCTGCTGCTGGGCGCGGGCGAGAGCGGC; AAGTCCACCTTCCTGAAGCAGATGCGGATCATCCACGGGCAGGACTTCGACCAGCNCGCG; CGCGAGGAGTTCCGCCCCACCATCTACAGCAACGTGATCAAAGGTATGAGGGTGCTGGTT; GATGCTCGAGAGAAGCTTCATATTCCCTGGGGAGACAACTCAAACCAACAACATGGAGAT; AAGATGATGTCGTTTGATACCCGGGCCCCCATGGCAGCCCAAGGAATGGTGGAAACAAGG; GTTTTCTTACAATATCTTCCTGCTATAAGAGCATTATGGGCAGACAGCGGCATACAGAAT; GCCTATGACCGGCGTCGAGAATTTCAACTGGGTGAATCTGTAAAATATTTCCTGGATAAC; TTGGATAAACTTGGAGAACCAGATTATATTCCATCACAACAAGATATTCTGCTTGCCAGA; AGACCCACCAAAGGCATCCATGAATACGACTTTGAAATAAAAAATGTTCCTTTCAAAATG; GTTGATGTAGGTGGTCAGAGATCAGAAAGGAAACGTTGGTTTGAATGTTTCGACAGTGTG; ACATCAATACTTTTCCTTGTTTCCTCAAGTGAATTTGACCAGGTGCTTATGGAAGATCGA; CTGACCAATCGCCTTACAGAGTCTCTGAACATTTTTGAAACAATCGTCAATAACCGGGTT; TTCAGCAATGTCTCCATAATTCTGTTCTTAAACAAGACAGACTTGCTTGAGGAGAAGGTG; CAAATTGTGAGCATCAAAGACTATTTCCTAGAATTTGAAGGGGATCCCCACTGCTTAAGA; GACGTCCAAAAATTCCTGGTGGAATGTTTCCGGAACAAACGCCGGGACCAGCAACAGAAG; CCCTTATACCACCACTTCACCACTGCTATCAACACGGAGAACATCCGCCTTGTTTTCCGT; GACGTGAAGGATACTATTCTGCATGACAACCTCAAGCAGCTTATGCTACAGTGA; ; Clone variation with respect to NM_006572.4; 236 g=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006572 unedited; CGCCCTGCGAGTCAGTTCGCTGGTTCCCTCCCTCCCTGGGCGCGCTCGGGCCGCCGCCGC; GCTCCCCGCCCTCGAGCCTCGGTGCCGGAGCCGCCCGCCGCCGGAGGAGGAGGTGGAGGG; AGCCGGAGGGGCCCGCCGAGGCGGCGGCGGCGGCGGCAAGATGGCGGACTTCCTGCCGTC; GCGGTCCGTGCTGTCCGTGTGCTTCCCCGGCTGCCTGCTGACGAGTGGCGAGGCCGAGCA; GCAACGCAAGTCCAAGGAGATCGACAAATGCCTGTCTCGGGAAAAGACCTATGTGAAGCG; GCTGGTGAAGATCCTGCTGCTGGGCGCGGGCGAGAGCGGCAAGTCCACCTTCCTGAAGCA; GATGCGGATCATCCACGGGCAGGACTTCGACCAGCCGCGCGCGAGGAGTTCCGCCCCACC; ATCTACAGCAACGTGATCAAAGGTATGAGGGTGCTGGTTGATGCTCGAGAGAAGCTTCAT; ATTCCCTGGGGAGACAACTCAAACCAACAACATGGAGATAAGATGATGTCGTTTGATACC; CGGGCCCCCATGGCAGCCCAAGGAATGGTGGAAACAAAGGGTTTTCTTACAATATCTTCC; TGCTATAAGAGCATTATGGGCAGACAGCGGCATACAGAATGCCTATGACCCGGCGTCGAG; AATTTCAACTGGGTGAATCTGTAAAATATTTNCTGGATAACTTGGGATNAACTTTGGGAG; AACCAGATTATNTTNCATCACAACAAGATATTTCTGCTTGNCAAAAGACCCNCCAAAGGC; ATNCATGAATACCACTTTTGAATAAAAAATGGTNCTTTCAAATGGGTGTGNGNGNGGGGG; GCAAAAATANAAGGNAAACTTTGGTTTTGAATGTTTC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006572 unedited; TTAGCTTGGACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGACTTTC; TCTAAATTTATTAGGGAGTTTGTTACAAATGTTTGGGCTTTACAGGCATGATTTCACGGA; TTCAAACAAGAAATTAACACTGATATTTAGCCTTCTCATGACATACACAGAAATAACATT; GCTACAAACTGCAATGGAGAGAATCTTGTTTCAAATGGCTTAGTTTGGGGTTTTGTCTAA; ATGTATCATTATATAATGAAAGCACCAATTTGAGGGTTTCTCAAATAGTGATTTGAATTT; TAGGACATAACAGTATAACATGGTAACTTTATTCTTCATATATAAATAAGGCATAATCGG; ATGTGTATTAATGCTGAAAATACATTTTATCAAAAGCATAAATACAAGTATTTGGGTACA; CATTGAAAGTTAGGACTTAACCAATTTCTTCTTACAAAAAATGATAAGGACATATGGTAN; TTTGTTGAATCTAAATCAGCACTTCTAATCATTTCTGGTATTAAGGCGATCTTATTTCCT; TCTACTTTAAACCAACCACTGCCACCAACTGAATAAGGATTTCCCTAAACAGCCTCAATT; TGAGAGACTAACCAGAGGACCTTCTCTATGCAAATTACATGCCAATACACCTTANAAGAA; AAATAGACAAAAGTGAATGAGTTTTTTCATTCCATATTGCTTTCTTTGGTTGCAGAGCAA; TCCAGTGCCCTTTCCCAGATATAAAATTGAAATGGAAGATTTCAATTTAATATTTTGGCA; GACAGCACCAATGCAGGTTATTAAAATATTAAAGGGGCAGAGGCATGCTTTGAATACTCA; GATTTCAGAAACATTTGCAAACCCCTTTCTTAAAAGNTATGGCTAAATATAGGTGGTTTA; TAGGGACTGAATACATTCCTCAAGCCATGAGCACCTTGGGGGATTCCCTCAAATAATGAA; AGCCTCCGATTAGACA;
• Restriction Sites: NotI-NotI
• ACCN: NM_006572
• Insert Size: 5090 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006572.3, NP_006563.2
• RefSeq Size: 4744 bp
• RefSeq ORF: 1134 bp
• Locus ID: 10672
• UniProt ID: Q14344
• Cytogenetics: 17q24.1
• Domains: G-alpha
• Protein Families: Druggable Genome
• Protein Pathways: Long-term depression, Regulation of actin cytoskeleton, Vascular smooth muscle contraction
• Summary: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems (PubMed:15240885, PubMed:16787920, PubMed:16705036, PubMed:27084452). Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF and ARHGEF12/LARG) (PubMed:15240885, PubMed:12515866). GNA13-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1) (By similarity). Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway (PubMed:16787920, PubMed:16705036, PubMed:27084452). Inhibits CDH1-mediated cell adhesion in process independent from Rho activation (PubMed:11976333).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.