GLO1 (NM_006708) Human Untagged Clone

CAT#: SC115924

GLO1 (untagged)-Human glyoxalase I (GLO1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GLO1
• Synonyms: GLOD1; GLYI; HEL-S-74
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115924 sequence for NM_006708 edited (data generated by NextGen Sequencing)
  ATGGCAGAACCGCAGCCCCCGTCCGGCGGCCTCACGGACGAGGCCGCCCTCAGTTGCTGC
  TCCGACGCGGACCCCAGTACCAAGGATTTTCTATTGCAGCAGACCATGCTACGAGTGAAG
  GATCCTAAGAAGTCACTGGATTTTTATACTAGAGTTCTTGGAATGACGCTAATCCAAAAA
  TGTGATTTTCCCATTATGAAGTTTTCACTCTACTTCTTGGCTTATGAGGATAAAAATGAC
  ATCCCTAAAGAAAAAGATGAAAAAATAGCCTGGGCGCTCTCCAGAAAAGCTACACTTGAG
  CTGACACACAATTGGGGCACTGAAGATGATGAGACCCAGAGTTACCACAATGGCAATTCA
  GACCCTCGAGGATTCGGTCATATTGGAATTGCTGTTCCTGATGTATACAGTGCTTGTAAA
  AGGTTTGAAGAACTGGGAGTCAAATTTGTGAAGAAACCTGATGATGGTAAAATGAAAGGC
  CTGGCATTTATTCAAGATCCTGATGGCTACTGGATTGAAATTTTGAATCCTAACAAAATG
  GCAACCTTAATGTAG
  
  Clone variation with respect to NM_006708.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006708 unedited
  GTAATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGGGTCGTCTGTGATACTGC
  AGCGCAGCCATGGCAGAACCGCAGCCCCCGTCCGGCGGCCTCACGGACGAGGCCGCCCTC
  AGTTGCTGCTCCGACGCGGACCCCAGTACCAAGGATTTTCTATTGCAGCAGACCATGCTA
  CGAGTGAAGGATCCTAAGAAGTCACTGGATTTTTATACTAGAGTTCTTGGAATGACGCTA
  ATCCAAAAATGTGATTTTCCCATTATGAAGTTTTCACTCTACTTCTTGGCTTATGAGGAT
  AAAAATGACATCCCTAAAGAAAAAGATGAAAAAATAGCCTGGGCGCTCTCCAGAAAAGCT
  ACACTTGAGCTGACACACAATTGGGGCACTGAAGATGATGAGACCCAGAGTTACCACAAT
  GGCAATTCAGACCCTCGAGGATTCGGTCATATTGGAATTGCTGTTCCTGATGTATACAGT
  GCTTGTAAAAGGTTTGAAGAACTGGGAGTCAAATTTGTGAAGAAACCTGATGATGGTAAA
  ATGAAAGGCCTGGCATTTATTCAAGATCCTGATGGCTACTGGATTGAAATTNTGAATCCT
  AACAAAATGGCAACCTTAATGTAGTGCTGTGAGAATTCTCCTTTGAGATTTCAGAAGAAA
  GGAAACAATGTGATTCAAGATATTTACATACCAGAAGCATCTAGGACTGATGGATCACTG
  TCCCCGATCNATTATTCTTCAGTCCATTTCCCCTTCCTATNTCAGCTGTTCCTTTTCACC
  TAACTGTTCAGTCATTCTGGTTTTCAAGCAGTGCTTTATCTCATGTCCTGAATATAGNTG
  NNGTACTTTATTTTTTAGTAATAATANAACAGTTCCCTTCAGAGCTGCATTTGCCTCTTC
  TGCCACTAAATATTNACTCCCTTCAATCTGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006708 unedited
  GGTTTTTCAGGGGNNCTNTNATNTATTCCAGTTCTCTCAAGCTATTGGCTATCTTGTCTT
  AAAGCACTCACATAAATCCATTTCACCCAAAAAGGAAACATAAAGTGCTTCTAGCAGTAC
  AAGCACGGTTGGCATGGCCTTTCCAAAGGTCTTCCACTAGAGTCTAGAGAAATCTAAATA
  TAGTCATCCACAAACTGGATGTTTTTATTTTCTGAGCCATTAGAGATTTTCAAAATCACT
  TTGATTTTTAAAAACTCATCAAATGTGAATCATGGCGGGGAAGACCACTGAGCTGATTTC
  TGATAACTAAGTTATCACTGAACATAATTTATCATATATGGCTACTGGCATCATGAAGAC
  CTTGGGATAGGGAAGACTCTTTATGAGAAATATAAACATCACTTGTGTAGGAATCACCAG
  GTGTCCCTAGAGCAGTTTTGTACTAAAGACTTCTAGTGTTTACTNCCTCACCACGGGTAC
  CTTCCCAACCNNNNNNNNNNNCNCCCCNNCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
  NCCCCCCCCCCCCCCCCCCCCNCCCCCCCCCCCCCCCCCCCCCCCCCCCCCNCCCCCCCC
  CCCNCCCCCNNNCCCCCCCCCCCCNCCNCCCCCCCCCCCCCCCCCCCCCCCCCCCCNCCC
  CCACCCCCNCCNACCCCCCCCCCCCCCCCCNCCCCCCNCCCCCCCCCCCCCCCCNCNCCC
  CCCCCCCCNCCCCCCCCCCCCNCCCCCCNCCAACACACCCCCCCCCCCCCCACCCCCCCC
  CCCACCCCCCCCCCCACCCACCCCCCACACAACCCCCACCCCCACCCACCCCACCCCCCA
  CACCCCCCCCACCCCCCCACACCACCCNCCCCCCCACNCCACCACCCCCCCACCACACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006708
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006708.1, NP_006699.1
• RefSeq Size: 1993 bp
• RefSeq ORF: 555 bp
• Locus ID: 2739
• UniProt ID: Q04760
• Cytogenetics: 6p21.2
• Domains: Glyoxalase
• Protein Pathways: Pyruvate metabolism
• Gene Summary: The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq, Jul 2008]