STIP1 (NM_006819) Human Untagged Clone

SKU SC115872

STIP1 (untagged)-Human stress-induced-phosphoprotein 1 (STIP1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: STIP1
• Synonyms: HEL-S-94n; HOP; IEF-SSP-3521; P60; STI1; STI1L
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115872 sequence for NM_006819 edited (data generated by NextGen Sequencing); ATGGAGCAGGTCAATGAGCTGAAGGAGAAAGGCAACAAGGCCCTGAGCGTGGGTAACATC; GATGATGCCTTACAGTGCTACTCCGAAGCTATTAAGCTGGATCCCCACAACCACGTGCTG; TACAGCAACCGTTCTGCTGCCTATGCCAAGAAAGGAGACTACCAGAAGGCTTATGAGGAT; GGCTGCAAGACTGTCGACCTAAAGCCTGACTGGGGCAAGGGCTATTCACGAAAAGCAGCA; GCTCTAGAGTTCTTAAACCGCTTTGAAGAAGCCAAGCGAACCTATGAGGAGGGCTTAAAA; CACGAGGCAAATAACCCTCAACTGAAAGAGGGTTTACAGAATATGGAGGCCAGGTTGGCA; GAGAGAAAATTCATGAACCCTTTCAACATGCCTAATCTGTATCAGAAGTTGGAGAGTGAT; CCCAGGACAAGGACACTACTCAGTGATCCTACCTACCGGGAGCTGATAGAGCAGCTACGA; AACAAGCCTTCTGACCTGGGCACGAAACTACAAGATCCCCGGATCATGACCACTCTCAGC; GTCCTCCTTGGGGTCGATCTGGGCAGTATGGATGAGGAGGAAGAGATTGCAACACCTCCA; CCACCACCCCCTCCCAAAAAGGAGACCAAGCCAGAGCCAATGGAAGAAGATCTTCCAGAG; AATAAGAAGCAGGCACTGAAAGAAAAAGAGCTGGGGAACGATGCCTACAAGAAGAAAGAC; TTTGACACAGCCTTGAAGCATTACGACAAAGCCAAGGAGCTGGACCCCACTAACATGACT; TACATTACCAATCAAGCAGCGGTATACTTTGAAAAGGGCGACTACAATAAGTGCCGGGAG; CTTTGTGAGAAGGCCATTGAAGTGGGGAGAGAAAACCGAGAAGACTATCGACAGATTGCC; AAAGCATATGCTCGAATTGGCAACTCCTACTTCAAAGAAGAAAAGTACAAGGATGCCATC; CATTTCTATAACAAGTCTCTGGCAGAGCACCGAACCCCAGATGTGCTCAAGAAATGCCAG; CAGGCAGAGAAAATCCTGAAGGAGCAAGAGCGGCTGGCCTACATAAACCCCGACCTGGCT; TTGGAGGAGAAGAACAAAGGCAACGAGTGTTTTCAGAAAGGGGACTATCCCCAGGCCATG; AAGCATTATACAGAAGCCATCAAAAGGAACCCGAAAGATGCCAAATTATACAGCAATCGA; GCTGCCTGCTACACCAAACTCCTGGAGTTCCAGCTGGCACTCAAGGACTGTGAGGAATGT; ATCCAGCTGGAGCCGACCTTCATCAAGGGTTATACACGGAAAGCCGCTGCGCTGGAAGCG; ATGAAGGACTACACCAAAGCCATGGATGTGTACCAGAAGGCGCTAGACCTGGACTCCAGC; TGTAAGGAGGCGGCAGACGGCTACCAGCGCTGTATGATGGCGCAGTACAACCGGCACGAC; AGCCCCGAAGATGTGAAGCGACGAGCCATGGCCGACCCTGAGGTGCAGCAGATCATGAGT; GACCCAGCCATGCGCCTTATCCTGGAACAGATGCAGAAGGACCCCCAGGCACTCAGCGAA; CACTTAAAGAATCCTGTAATAGCACAGAAGATCCAGAAGCTGATGGATGTGGGTCTGATT; GCAATTCGGTGA; ; Clone variation with respect to NM_006819.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006819 unedited; GTTANCATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCGGCGCGTG; CGGTTGGGAACGCGGAGCGGACGGATTCGATTCAACGGGGTTCCGGACCGCGCTGCGCTA; TGGAGCAGGTCAATGAGCTGAAGGAGAAAGGCAACAAGGCCCTGAGCGTGGGTAACATCG; ATGATGCCTTACAGTGCTACTCCGAAGCTATTAAGCTGGATCCCCACAACCACGTGCTGT; ACAGCAACCGTTCTGCTGCCTATGCCAAGAAAGGAGACTACCAGAAGGCTTATGAGGATG; GCTGCAAGACTGTCGACCTAAAGCCTGACTGGGGCAAGGGCTATTCACGAAAAGCAGCAG; CTCTAGAGTTCTTAAACCGCTTTGAAGAAGCCAAGCGAACCTATGAGGAGGGCTTAAAAC; ACGAGGCAAATAACCCTCAACTGAAAGAGGGTTTACAGAATATGGAGGCCAGGTTGGCAG; AGAGAAAATTCATGAACCCTTTCAACATGCCTAATCTGTATCAGAAGTTGGAGAGTGATC; CCAGGACAAGGACACTACTCAGTGATCCTACCTACCGGGAGCTGATAGAGCAGCTACGAA; ACAAGCCTTCTGACCTGNGCACGAAACTACAAGATCCCCGGATCATGACCACTCTCAGCG; TCCTCCTTGGGGTCGATCTGGGCAGTATGGATGAGGAGGAAGAGATTGCAACACCTNCAC; CACCNACCCCTCCCAAAAAGGAGACCAAGCCAGAGCCAATGGAAGAAGATCTTCCAGAGA; ATAAGAAGCAGGCACTGAAAGACAAAGACTGGGGAACGATGCCCTACAGAAAGAAGACCT; TGACACAGCCTTGAAGCATTACGACAAAGCCAAGGAGCTGGACCCCNACTACATGACTTA; CATTACCAATCAGCAGCGGTATACTTTGAN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006819 unedited; AGCTATGNCCGCGGCCGCAATCTAGNAGTCGGTTTTTTTTTTTTTTTTTTAACCACGCCC; CAACTGGGGCTTGTTTTATTGGGAGAAAGGGGGACCGCAGAATAAACAACGTGAGACAGC; TGGGACCTGAGGCTGGAAGAACCCCCTCCCCTCCCCATAACATGCCCACTGCCCCAAATA; AAAAAAAAACCAACTATGGGAGAGCAGCCGAGACAGCAACTAGGGGCAGGGGAAAGAGAC; ATGGAACTCGAGGGCAGCGGTGAAGAGACCATGCGTGTGCTGGGAGGGCCCAGAGGCAGC; GGCGGCACAAACAGCGCAGGTACGAGTCTCTGTGTCTTCCCCGGGGTTATGTATAAATAT; AGAGAGATGAGGCCTTCTCTCCCCTGCTCTCCCTTCCGCTCCGTGCTGCTCGCCGCGGTC; CCAGCTCCTCTTTCCACATGAGGGCGAAGGGAAGGGGGGATGAACAAGTCATCACCGAAT; TGCAATCAGACCCACATCCATCAGCTTCTGGATCTTCTGTGCTATTACAGGATTCTTTAA; GTGTTCGCTGAGTGCCTGGGGGTCCTTCTGCATCTGTTCCAGGATAAGGCGCATGGCTGG; GTCACTCATGATCTGCTGCACCTCAGGGTCGGCCATGGCTCGTCGCTTCACATCTTCGGG; GCTGTCGTGCCGGTTGTACTGCGCCATCATACAGCGCTGGTAGCCGTCTGCCGCCTCCTT; ACAGCTGGAGTCCAGGTCTAGCGCCTTCTGGTACACATCCATGGCTTTGGTGTAGTCCTT; CATCGCTTCCAGCGCAGCGGCTTTCCGTGTATACCCTTGATGAAGGGTCGCTCAGCTGGA; ACATNNCTCACATCCTTTGATGCAGCTGGAACTCCAGAGTTGGTGTACAGGCGCTCGAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_006819
• Insert Size: 2440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006819.1, NP_006810.1
• RefSeq Size: 2113 bp
• RefSeq ORF: 1632 bp
• Locus ID: 10963
• UniProt ID: P31948
• Cytogenetics: 11q13.1
• Domains: STI1, TPR
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Prion diseases
• Summary: STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]).[supplied by OMIM, Jul 2009]; Transcript Variant: This variant (2) uses an alternate 5' terminal exon and thus differs in the 5' UTR and 5' coding region, and uses an alternate start codon, compared to variant 1. The encoded isoform (b) has a distinct N-terminus and is shorter than isoform a.