ELF2 (NM_006874) Human Untagged Clone

CAT#: SC115824

ELF2 (untagged)-Human E74-like factor 2 (ets domain transcription factor) (ELF2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ELF2
• Synonyms: b; EU32; NERF; NERF-1A; NERF-1a; NERF-1B; NERF-2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006874 edited
  GAATTCGGCACGAGGCCGGCCGCGGGTTCCGCCTCCTCCCCTGCCGCCGCTGCTCACGGT
  GTAAGTCAATGTGAAGCAGCAGCTCCAGCCCCGGGATAAACATGGCGACGTCTCTGCATG
  AGGGACCCACGAACCAGCTGGATCTGCTCATCCGGGCCGTGGAAGCATCAGTTCACAGCA
  GTAATGCACACTGTACAGATAAGACAATTGAAGCTGCTGAAGCCCTGCTTCATATGGAAT
  CTCCTACCTGCTTGAGGGATTCAAGAAGTCCTGTGGAAGTGTTTGTTCCTCCTTGTGTAT
  CAACTCCAGAATTCATCCATGCTGCTATGAGGCCAGATGTCATTACAGAAACTGTAGTGG
  AGGTGTCAACTGAAGAGTCTGAACCCATGGATACCTCTCCTATTCCAACATCACCAGATA
  GCCATGAACCAATGAAAAAGAAAAAAGTTGGCCGTAAACCAAAGACCCAGCAATCACCAA
  TTTCCAATGGGTCTCCTGAGTTAGGTATAAAGAAGAAACCAAGAGAAGGAAAAGGAAACA
  CAACCTATTTGTGGGAGTTTCTTTTAGATCTACTTCAAGATAAAAATACTTGTCCCAGGT
  ATATTAAATGGACTCAGAGAGAAAAAGGCATATTCAAGCTGGTGGATTCAAAGGCTGTCT
  CTAAGCTTTGGGGAAAGCATAAGAACAAACCAGACATGAACTATGAAACCATGGGACGAG
  CTTTGAGATACTACTACCAAAGGGGAATTCTTGCAAAGGTTGAAGGACAGAGGCTTGTAT
  ATCAGTTCAAGGATATGCCGAAAAACATAGTGGTCATAGATGATGACAAAAGTGAAACCT
  GTAATGAAGATTTAGCAGGAACTACTGATGAAAAATCATTAGAACGAGTGTCACTGTCTG
  CAGAAAGTCTCCTGAAAGCAGCATCCTCTGTTCGCAGTGGAAAAAATTCATCCCCTATAA
  ACTGCTCCAGAGCAGAGAAGGGTGTAGCTAGAGTTGTGAATATCACTTCCCCTGGGCACG
  ATGCTTCATCCAGGTCTCCTACTACCACTGCATCTGTGTCAGCAACAGCAGCTCCAAGGA
  CAGTTCGTGTGGCAATGCAGGTACCTGTTGTAATGACATCATTGGGTCAGAAAATTTCAA
  CTGTGGCAGTTCAGTCAGTTAATGCAGGTGCACCATTAATAACCAGCACTAGTCCAACAA
  CAGCGACCTCTCCAAAGGTAGTCATTCAGACAATCCCTACTGTGATGCCAGCTTCTACTG
  AAAATGGAGACAAAATCACCATGCAGCCTGCCAAAATTATTACCATCCCAGCTACACAGC
  TTGCACAGTGTCAACTGCAGACAAAGTCAAATCTGACTGGATCAGGAAGCATTAACATTG
  TTGGAACCCCATTGGCTGTGAGAGCACTTACCCCTGTTTCAATAGCCCATGGTACACCTG
  TAATGAGACTATCAATGCCTACTCAGCAGGCATCTGGCCAGACTCCTCCTCGAGTTATCA
  GTGCAGTCATAAAGGGGCCAGAGGTTAAATCGGAAGCAGTGGCAAAAAAGCAAGAACATG
  ATGTGAAAACTTTGCAGCTAGTAGAAGAAAAACCAGCAGATGGAAATAAGACAGTGACCC
  ACGTAGTGGTTGTCAGTGCGCCTTCAGCTATTGCCCTTCCTGTAACTATGAAAACAGAAG
  GACTAGTGACATGTGAGAAATAAAATAGCAGCTCCACCATGGACTTCAGGCTGTTAGTGG
  CAGTACTGACATAAACATTTGCAAGGGAAGTCATCAAGAAAAGTCAAAGAAGACTTTAAA
  ACATTTTTAATGCATATACAAAAACAATCAGACTTACTGGAAATAAATTACCTATCCCAT
  GTTTCAGTGGGAAATGAACTACATATTGAGATGCTGACAGAAAACTGCCTCTTACAGTAG
  GAAACAACTGAACCCATCAATAAGAAAAAGGATCGAAAGGGACCAAGCAGCTCACTACGA
  TATCAAGTTACACTAAGACTTGGAACACTAACATTCTGTAAGAGGTTATATAGTTTTCAG
  TGGGAGGGGTTGGGATGGGTAATCTCATTGTTACATATAGCAATTTTTGATGCATTTTAT
  ATGCATACCAGCAATTATTACTGTGTTCGCACAGTTCTCACTTAACTGGTGCTATGTGAA
  GACTCTGCTAATATAGGTATTTTAGAATGTGAATTGAAGAATGGATCCCAAAAACTTCAG
  AAAGAGGATAGCAAAAAAAGATCTAGTGCGATTTTATATATATATATATATATATATACA
  TACATATATATATATCATATAGCTTAAGCTGATTTAAAACAAAGGCCTTAGACTAATTTT
  CGATTTTCTTTCTTGAAATAAGCTAATGGCTTGTTTGTGTAAAGCTTTTTTATTAAAAGA
  AAAATTTTAAAAATCTAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006874 unedited
  GGTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCGGCCGCGG
  GTTCCGCCTCCTCCCCTGCCGCCGCTGCTCACGGTGTAAGTCAATGTGAAGCAGCAGCTC
  CAGCCCCGGGATAAACATGGCGACGTCTCTGCATGAGGGACCCACGAACCAGCTGGATCT
  GCTCATCCGGGCCGTGGAAGCATCAGTTCACAGCAGTAATGCACACTGTACAGATAAGAC
  AATTGAAGCTGCTGAAGCCCTGCTTCATATGGAATCTCCTACCTGCTTGAGGGATTCAAG
  AAGTCCTGTGGAAGTGTTTGTTCCTCCTTGTGTATCAACTCCAGAATTCATCCATGCTGC
  TATGAGGCCAGATGTCATTACAGAAACTGTAGTGGAGGTGTCAACTGAAGAGTCTGAACC
  CATGGATACCTCTCCTATTCCAACATCACCAGATAGCCATGAACCAATGAAAAAGAAAAA
  AGTTGGCCGTAAACCAAAGACCCAGCAATCACCAATTTCCAATGGGTCTCCTGAGTTAGG
  TATAAAGAAGAAACCAAGAGAAGGAAAAGGAAACACAACCTATTTGTGGGAGTTTCTTTT
  AGATCTACTTCAAGATAAAAATACTTGTCCCAGGTATATTAAATGGACTCAGAGAGAAAA
  AGGCATATTCAAGCTGGTGGATTCAAAGGCTGTCTCTAAGCTCTGGGGAAAGCATAAGAA
  CCAACCAGACATGAACTATGAAACCATGGGACGAGCTTTGAGATACTACTACCAAAGGGG
  AAATTCTGCCAAGGTTGAACGACAGAGGCTTGTATATCAGTTCCAGGATATGCCCGAAAA
  CATAGTGGTCATAGATGATGACACAGTGAAACCCTGTATGAAGATTTACCAGGACCTACT
  GATGGAAAATCATTAGAACGAGTGCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006874 unedited
  ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAGATTTTTAAAATTTTT
  CTTTTAATAAAAAAGCTTTACACAAACAAGCCATTAGCTTATTTCAAGAAAGAAAATCGA
  AAATTAGTCTAAGGCCTTTGTTTTAAATCAGCTTAAGCTATATGATATATATATATGTAT
  GTATATATATATATATATATATATAAAATCGCACTAGATCTTTTTTTGCTATCCTCTTTC
  TGAAGTTTTTGGGATCCATTCTTCAATTCACATTCTAAAATACCTATATTAGCAGAGTCT
  TCACATAGCACCAGTTAAGTGAGAACTGTGCGAACACAGTAATAATTGCTGGTATGCATA
  TAAAATGCATCAAAAATTGCTATATGTAACAATGAGATTACCCATCCCAACCCCTCCCAC
  TGAAAACTATATAACCTCTTACAGAATGTTAGTGTTCCAAGTCTTAGTGTAACTTGATAT
  CGTAGTGAGCTGCTTGGTCCCTTTCGATCCTTTTTCTTATTGATGGGTTCAGTTGTTTCC
  TACTGTAAGAGGCAGTTTTCTGTCAGCATCTCAATATGTAGTTCATTTCCCACTGAAACA
  TGGGATAGGTAATTTATTTCCAGTAAGTCTGATTGTTTTTGTATATGCATTAAAAATGTT
  TTAAAGTCTTCTTTGACTTTTCTTGATGACTTCCCTTNGCAATGTTTATGTCAGTACTGC
  CACTAACAGCCCTGAAGTCATGGTGGAGCTGCTATNTTATTTCTCACATGTCACTAGCCC
  CTTCTGGTTTCATAGNTACAGGAAGGGCCAATAGCTGGAGGCGCACTGACAACCACCTAC
  GGGGGCCACTGGCCTTATTCCCTCTGGCTGGGTTTTCTNCTACAAGCCTGCAAGTTTCAC
  CTCATGGTTTTGGTTTTTTGCCACTGCTTCGATTAACCCTCGCCCCCTTAGAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006874
• Insert Size: 2430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006874.2, NP_006865.1
• RefSeq Size: 3171 bp
• RefSeq ORF: 1602 bp
• Locus ID: 1998
• UniProt ID: Q15723
• Cytogenetics: 4q31.1
• Domains: ETS
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: Isoform 1 transcriptionally activates the LYN and BLK promoters and acts synergistically with RUNX1 to transactivate the BLK promoter.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has an alternate exon in place of the first three exons and uses an alternate in-frame splice junction at the 5' end of an internal exon compared to variant 1. Translation starts in the alternate exon, so the resulting isoform (2) has a shorter and distinct N-terminus and contains an alternate internal segment compared to isoform 1.