FMO3 (NM_006894) Human Untagged Clone

SKU SC115806

FMO3 (untagged)-Human flavin containing monooxygenase 3 (FMO3), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: FMO3
• Synonyms: dJ127D3.1; FMOII; TMAU
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115806 sequence for NM_006894 edited (data generated by NextGen Sequencing); ATGGGGAAGAAAGTGGCCATCATTGGAGCTGGTGTGAGTGGCTTGGCCTCCATCAGGAGC; TGTCTGGAAGAGGGGCTGGAGCCCACCTGCTTTGAGAAGAGCAATGACATTGGGGGCCTG; TGGAAATTTTCAGACCATGCAGAGGAGGGCAGGGCTAGCATTTACAAATCAGTCTTTTCC; AACTCTTCCAAAGAGATGATGTGTTTCCCAGACTTCCCATTTCCCGATGACTTCCCCAAC; TTTATGCACAACAGCAAGATCCAGGAATATATCATTGCATTTGCCAAAGAAAAGAACCTC; CTGAAGTACATACAATTTAAGACATTTGTATCCAGTGTAAATAAACATCCTGATTTTGCA; ACTACTGGCCAGTGGGATGTTACCACTGAAAGGGATGGTAAAAAAGAATCGGCTGTCTTT; GATGCTGTAATGGTTTGTTCCGGACATCATGTGTATCCCAACCTACCAAAAGAGTCCTTT; CCAGGACTAAACCACTTTAAAGGCAAATGCTTCCACAGCAGGGACTATAAAGAACCAGGT; GTATTCAATGGAAAGCGTGTCCTGGTGGTTGGCCTGGGGAATTCGGGCTGTGATATTGCC; ACAGAACTCAGCCGCACAGCAGAACAGGTCATGATCAGTTCCAGAAGTGGCTCCTGGGTG; ATGAGCCGGGTCTGGGACAATGGTTATCCTTGGGACATGCTGCTCGTCACTCGATTTGGA; ACCTTCCTCAAGAACAATTTACCGACAGCCATCTCTGACTGGTTGTACATGAAGCAGATG; AATGCAAGATTCAAGCATGAAAACTATGGCTTGATGCCTTTAAATGGAGTCCTGAGGAAA; GAGCCTGTATTTAACGATGAGCTCCCAGCAAGCATTCTGTGTGGCATTGTGTCCGTAAAG; CCTAACGTGAAGGAATTCACAGAGACCTCGGCCATTTTTGAGGATGGGACCATATTTGAG; GGCATTGACTGTGTAATCTTTGCAACAGGGTATAGTTTTGCCTACCCCTTCCTTGATGAG; TCTATCATCAAAAGCAGAAACAATGAGATCATTTTATTTAAAGGAGTATTTCCTCCTCTA; CTTGAGAAGTCAACCATAGCAGTGATTGGCTTTGTCCAGTCCCTTGGGGCTGCCATTCCC; ACAGTTGACCTCCAGTCCCGCTGGGCAGCACAAGTAATAAAGGGAACTTGTACTTTGCCT; TCTATGGAAGACATGATGAATGATATTAATGAGAAAATGGAGAAAAAGCGCAAATGGTTT; GGCAAAAGCGAGACCATACAGACAGATTACATTGTTTATATGGATGAACTCTCCTCCTTC; ATTGGGGCAAAGCCCAACATCCCATGGCTGTTTCTCACAGATCCCAAATTGGCCATGGAA; GTTTATTTTGGCCCTTGTAGTCCCTACCAGTTTAGGCTGGTGGGCCCAGGGCAGTGGCCA; GGAGCCAGAAATGCCATACTGACCCAGTGGGACCGGTCGTTGAAACCCATGCAGACACGA; GTGGTCGGGAGACTTCAGAAGCCTTGCTTTTTTTTCCATTGGCTGAAGCTCTTTGCAATT; CCTATTCTGTTAATCGCTGTTTTCCTTGTGTTGACCTAA; ; Clone variation with respect to NM_006894.5; 769 g=>a;1530 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006894 unedited; GCACCAGAAGGTTGGACAGGACGTAGACACACAGAAGAAAAGAAGACAAAGAACGGGTAG; GAAAATTAAAAAGGTTACCATGGNGGAAGAAAGTGGCCATCATTGGAGCTGGTGTGAGTG; GCTTGGCCTCCATCAGGAGCTGTCTGGAAGAGGGGCTGGAGCCCACCTGCTTTGAGAAGA; GCAATGACATTGGGGGCCTGTGGAAATTTTCAGACCATGCAGAGGAGGGCAGGGCTAGCA; TTTACAAATCAGTCTTTTCCAACTCTTCCAAAGAGATGATGTGTTTCCCAGACTTCCCAT; TTCCCGATGACTTCCCCAACTTTATGCACAACAGCAAGATCCAGGAATATATCATTGCAT; TTGCCAAAGAAAAGAACCTCCTGAAGTACATACAATTTAAGACATTTGTATCCAGTGTAA; ATAAACATCCTGATTTTGCAACTACTGGCCAGTGGGATGTTACCACTGAAAGGGATGGTA; AAAAAGAATCGGCTGTCTTTGATGCTGTAATGGTTTGTTCCGGACATCATGTGTATCCCA; ACCTACCAAAAGAGTCCTTTCCAGGACTAAACCACTTTAAAGGCAAATGCTTCCACAGCA; GGGACTATAAAGAACCAGGTGTATTCAATGGAAAGCGTGTCCTGGNTGGTTGGCCTGGGG; AAATTCGGCTGTGATATTGCCACAGAACTCAGCCGCACAGCAGAAACAGGTCATGATCAG; TTNCCAGAAGTGGCTCCTGGGT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006894 unedited; NNNNTTTACTTGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTATAATTATATCTGCTTAATTTGCTCTTTATTTAAATTTAAAATACATCTTT; CATACATTGTTTAAAAGGAAAAAGGAATCATGTCAAAATGTTTCAGGAAATGATTTAAGT; TAAATATAAAGAACATAGTTCTGATTTTGAAGTGTTAGTGGAACTCAAACAGAAATGATT; AGTGCTCTCTTAAGATCATGACATGATTCTTAAAGTGGCTATTTCAAAGCCTAAAAATAA; CACTGTATTACTGACTAATGTCTACAGAGTACTGCAAAAGATGCTGAATAGAAAAGCAGG; TGGTGTGAAAATTTTAATTTTTAAATAGCAAAGCCCCTGTCTGGGTATTGTCAGTAACTT; TCAAAAATCCTAGAGAAAATGATGATTAGGTCAACACAAGGAAAACAGCGATTAACAGAA; TAGGAATTGCAAAGAGCTTCAGCCAATGGAAAAAAAAGCAAGGCTTCTGAAGTCTCCCGA; CCACTCGTGTCTGCATGGGTTTCAACGACCGGTCCCACTGGGTCAGTATGGCATTTCTGG; CTCCTGGCCACTGCCCTGGGCCCACCAGCCTAAACTGGTAGGGACTACAAGGGCCAAAAT; AAACTTCCATGGNCCAATTTGGGATCTGTGAGAAACAGCCATGGGATGTTGGGCTTTGCC; CCAATGAAGGAGGGAGAGTTCATCCATATAAACAATGTAATCTGTCTGTATGGTCTCGCT; TTTGCCAAACATTTGCNGCTTTTCTNCTTNTTCTCATTAATATCATTNCATCATGTCTCC; ATAGAAGGGCAAGTACAGTTCCTTTTATTACTGTGCTGCCCAGCGGACTGGAGGGTCACT; GTGGAATGCAGCCCCAGGGACTGGACAAGCCATACTGCTATGGTGACTTTC;
• Restriction Sites: NotI-NotI
• ACCN: NM_006894
• Insert Size: 2140 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006894.4, NP_008825.4
• RefSeq Size: 2053 bp
• RefSeq ORF: 1599 bp
• Locus ID: 2328
• UniProt ID: P31513
• Cytogenetics: 1q24.3
• Domains: FMO-like
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Drug metabolism - cytochrome P450
• Summary: Flavin-containing monooxygenases (FMO) are an important class of drug-metabolizing enzymes that catalyze the NADPH-dependent oxygenation of various nitrogen-,sulfur-, and phosphorous-containing xenobiotics such as therapeutic drugs, dietary compounds, pesticides, and other foreign compounds. The human FMO gene family is composed of 5 genes and multiple pseudogenes. FMO members have distinct developmental- and tissue-specific expression patterns. The expression of this FMO3 gene, the major FMO expressed in adult liver, can vary up to 20-fold between individuals. This inter-individual variation in FMO3 expression levels is likely to have significant effects on the rate at which xenobiotics are metabolised and, therefore, is of considerable interest to the pharmaceutical industry. This transmembrane protein localizes to the endoplasmic reticulum of many tissues. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. Mutations in this gene cause the disorder trimethylaminuria (TMAu) which is characterized by the accumulation and excretion of unmetabolized trimethylamine and a distinctive body odor. In healthy individuals, trimethylamine is primarily converted to the non odorous trimethylamine N-oxide.[provided by RefSeq, Jan 2016]; Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Variants 1 and 2 both encode the same isoform (a).