Sumo 3 (SUMO3) (NM_006936) Human Untagged Clone

SKU SC115792

SUMO3 (untagged)-Human SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae) (SUMO3)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Sumo 3
• Synonyms: SMT3A; Smt3B; SMT3H1; SUMO-3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115792 sequence for NM_006936 edited (data generated by NextGen Sequencing); ATGTCCGAGGAGAAGCCCAAGGAGGGTGTGAAGACAGAGAATGACCACATCAACCTGAAG; GTGGCCGGGCAGGACGGCTCCGTGGTGCAGTTCAAGATCAAGAGGCACACGCCGCTGAGC; AAGCTGATGAAGGCCTACTGCGAGAGGCAGGGCTTGTCAATGAGGCAGATCAGATTCAGG; TTCGACGGGCAGCCAATCAATGAAACTGACACTCCAGCACAGCTGGAGATGGAGGACGAG; GACACCATCGACGTGTTCCAGCAGCAGACGGGAGGTGTGCCGGAGAGCAGCCTGGCAGGG; CACAGTTTCTAG; ; Clone variation with respect to NM_006936.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006936 unedited; TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGGAGAGCGGCGGGG; CCGAGAGCGTGACTCGCCCGCTCCGCGCTGCTTCCCCCGCGCCGCCTCCCCGCGCCGCTC; GCGCAGCCATGTCCGAGGAGAAGCCCAAGGAGGGTGTGAAGACAGAGAATGACCACATCA; ACCTGAAGGTGGCCGGGCAGGACGGCTCCGTGGTGCAGTTCAAGATCAAGAGGCACACGC; CGCTGAGCAAGCTGATGAAGGCCTACTGCGAGAGGCAGGGCTTGTCAATGAGGCAGATCA; GATTCAGGTTCGACGGGCAGCCAATCAATGAAACTGACACTCCAGCACAGCTGGAGATGG; AGGACGAGGACACCATCGACGTGTTCCAGCAGCAGACGGGAGGTGTGCCGGAGAGCAGCC; TGGCAGGGCACAGTTTCTAGAGGGCCCGTCCCCAGCCCGGGCCGTCCATCCTCGCATTGC; TGTTGAATGGTGAGCACGTGACCATGCCGACCACAAAGGTGTCTGCGGAAACTCGAGGAC; ATTCACCACGATGATTTTCCTCTCTTTGATGTACTTCAAGTGCAACTCAAAACTATATCT; GCAGGGATGAATCTGTAACTTAAATTGGGCCAATCAGAATTGTTATCTTTGTTCAGGTAA; AATGAGTTGCAAGATATTGTGGGTACTTTTGTGTGCTCATTTGTGTTTTCCCCCCCTCCT; ACACATTTTTTTAACCCCAAATTATAGCCTGAATGTTCGCTTTTAGTCTGCCAGGGATCT; GACTNCTGAGTTGGTTGCCTCTCCCCTGCTCACTCCAGTACATAAGNAATTGGTGTTNNC; CGCAGTGGGATGCNAGCTGTGGACAGGTATTGGGGGCAGGGTGGTAGGGAAGACAGACTG; TC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006936 unedited; ACCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTCTTTTACCAAACTATAT; TTACTGTTTACAGTAGTAAAGGACAACAAATAATGTACAAATTGGTGAATAAACACAACA; GAGCCAACAAACATCCCACCCGAGCCCATACAGCAAACAGGAAATGAGAACATTTCAGCA; AGATTTCAAGCAAGCAACAGATGATGGGTCATTGTTCAGGTGACTGTAAAAAGGCAGAGA; ATGGCCACCAAGCAGCAATGGAGCCTCAGGGAAGGACAATAGGCAGAACTATGAAAATGT; TTAATTGGTATAGATCCCAAAATATTTCACAGAACTGAAATCACCGGACTAATGCCATAA; ATTCAATACCTATTTGGAAAGCAGCACGGCACAAACCCACGAGAAGAACATGAAGTCATC; ATTCCATGGGTTCATTTGTAACCACACTTCGGAAGTATCTCTTATACACCGACAACCTAA; ACTGCAAATTCAGCAATACTTAGACCTACCTTTTTTATGCATAGACCATAATCATTCATT; AGAAGCAAATACTATAAGAAAATCTGGGGCCCCAACACCATTACCAAAGGAGAAATGGTG; GAACTTTCTTCATTTTCAACAGGGAAAAAAAGGCCCCATTTTGGTTGGCCCATTACTTGC; CTGGGAACCACCCGCCGATACTGACACAGGAAATGGGGTGGGATGGGTGCACAGGGGGTT; TGAACCTCCCGCTTCTCTCGTCGCGTTTCTTACTCTGTGCCTTACCGGATCCGCACCGAC; TGATATCAGTAGAATACGGTAGGGGACTCCCCGCGCGGTAGAGAGTACGGCGGCGCGCTC; TACGCCGTCTCGTCTTCTCCTCCTTGCTTTGTACCTTTGTCGTCCTACCTTCTTCGCGGG; AGATACTAACAATACGACATATAACTCGGCTAAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_006936
• Insert Size: 1770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006936.2, NP_008867.2
• RefSeq Size: 1831 bp
• RefSeq ORF: 312 bp
• Locus ID: 6612
• UniProt ID: P55854
• Cytogenetics: 21q22.3
• Domains: UBQ
• Protein Families: Druggable Genome
• Summary: This gene encodes a member of the small ubiquitin-related modifier (SUMO) family of eukaryotic proteins. The encoded protein is covalently conjugated to other proteins via a post-translation modification known as sumoylation. Sumoylation may play a role in a wide variety of cellular processes, including nuclear transport, DNA replication and repair, mitosis, transcriptional regulation, and signal transduction. Alternatively spliced transcript variants encoding distinct proteins have been described. [provided by RefSeq, Feb 2014]; Transcript Variant: This variant (1) represents the predominant transcript and encodes the shorter isoform (1).