Sumo 3 (SUMO3) (NM_006936) Human Untagged Clone

CAT#: SC115792

SUMO3 (untagged)-Human SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae) (SUMO3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Sumo 3
• Synonyms: SMT3A; Smt3B; SMT3H1; SUMO-3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115792 sequence for NM_006936 edited (data generated by NextGen Sequencing)
  ATGTCCGAGGAGAAGCCCAAGGAGGGTGTGAAGACAGAGAATGACCACATCAACCTGAAG
  GTGGCCGGGCAGGACGGCTCCGTGGTGCAGTTCAAGATCAAGAGGCACACGCCGCTGAGC
  AAGCTGATGAAGGCCTACTGCGAGAGGCAGGGCTTGTCAATGAGGCAGATCAGATTCAGG
  TTCGACGGGCAGCCAATCAATGAAACTGACACTCCAGCACAGCTGGAGATGGAGGACGAG
  GACACCATCGACGTGTTCCAGCAGCAGACGGGAGGTGTGCCGGAGAGCAGCCTGGCAGGG
  CACAGTTTCTAG
  
  Clone variation with respect to NM_006936.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006936 unedited
  TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGGAGAGCGGCGGGG
  CCGAGAGCGTGACTCGCCCGCTCCGCGCTGCTTCCCCCGCGCCGCCTCCCCGCGCCGCTC
  GCGCAGCCATGTCCGAGGAGAAGCCCAAGGAGGGTGTGAAGACAGAGAATGACCACATCA
  ACCTGAAGGTGGCCGGGCAGGACGGCTCCGTGGTGCAGTTCAAGATCAAGAGGCACACGC
  CGCTGAGCAAGCTGATGAAGGCCTACTGCGAGAGGCAGGGCTTGTCAATGAGGCAGATCA
  GATTCAGGTTCGACGGGCAGCCAATCAATGAAACTGACACTCCAGCACAGCTGGAGATGG
  AGGACGAGGACACCATCGACGTGTTCCAGCAGCAGACGGGAGGTGTGCCGGAGAGCAGCC
  TGGCAGGGCACAGTTTCTAGAGGGCCCGTCCCCAGCCCGGGCCGTCCATCCTCGCATTGC
  TGTTGAATGGTGAGCACGTGACCATGCCGACCACAAAGGTGTCTGCGGAAACTCGAGGAC
  ATTCACCACGATGATTTTCCTCTCTTTGATGTACTTCAAGTGCAACTCAAAACTATATCT
  GCAGGGATGAATCTGTAACTTAAATTGGGCCAATCAGAATTGTTATCTTTGTTCAGGTAA
  AATGAGTTGCAAGATATTGTGGGTACTTTTGTGTGCTCATTTGTGTTTTCCCCCCCTCCT
  ACACATTTTTTTAACCCCAAATTATAGCCTGAATGTTCGCTTTTAGTCTGCCAGGGATCT
  GACTNCTGAGTTGGTTGCCTCTCCCCTGCTCACTCCAGTACATAAGNAATTGGTGTTNNC
  CGCAGTGGGATGCNAGCTGTGGACAGGTATTGGGGGCAGGGTGGTAGGGAAGACAGACTG
  TC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006936 unedited
  ACCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTCTTTTACCAAACTATAT
  TTACTGTTTACAGTAGTAAAGGACAACAAATAATGTACAAATTGGTGAATAAACACAACA
  GAGCCAACAAACATCCCACCCGAGCCCATACAGCAAACAGGAAATGAGAACATTTCAGCA
  AGATTTCAAGCAAGCAACAGATGATGGGTCATTGTTCAGGTGACTGTAAAAAGGCAGAGA
  ATGGCCACCAAGCAGCAATGGAGCCTCAGGGAAGGACAATAGGCAGAACTATGAAAATGT
  TTAATTGGTATAGATCCCAAAATATTTCACAGAACTGAAATCACCGGACTAATGCCATAA
  ATTCAATACCTATTTGGAAAGCAGCACGGCACAAACCCACGAGAAGAACATGAAGTCATC
  ATTCCATGGGTTCATTTGTAACCACACTTCGGAAGTATCTCTTATACACCGACAACCTAA
  ACTGCAAATTCAGCAATACTTAGACCTACCTTTTTTATGCATAGACCATAATCATTCATT
  AGAAGCAAATACTATAAGAAAATCTGGGGCCCCAACACCATTACCAAAGGAGAAATGGTG
  GAACTTTCTTCATTTTCAACAGGGAAAAAAAGGCCCCATTTTGGTTGGCCCATTACTTGC
  CTGGGAACCACCCGCCGATACTGACACAGGAAATGGGGTGGGATGGGTGCACAGGGGGTT
  TGAACCTCCCGCTTCTCTCGTCGCGTTTCTTACTCTGTGCCTTACCGGATCCGCACCGAC
  TGATATCAGTAGAATACGGTAGGGGACTCCCCGCGCGGTAGAGAGTACGGCGGCGCGCTC
  TACGCCGTCTCGTCTTCTCCTCCTTGCTTTGTACCTTTGTCGTCCTACCTTCTTCGCGGG
  AGATACTAACAATACGACATATAACTCGGCTAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006936
• Insert Size: 1770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006936.2, NP_008867.2
• RefSeq Size: 1831 bp
• RefSeq ORF: 312 bp
• Locus ID: 6612
• UniProt ID: P55854
• Cytogenetics: 21q22.3
• Domains: UBQ
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the small ubiquitin-related modifier (SUMO) family of eukaryotic proteins. The encoded protein is covalently conjugated to other proteins via a post-translation modification known as sumoylation. Sumoylation may play a role in a wide variety of cellular processes, including nuclear transport, DNA replication and repair, mitosis, transcriptional regulation, and signal transduction. Alternatively spliced transcript variants encoding distinct proteins have been described. [provided by RefSeq, Feb 2014]
  Transcript Variant: This variant (1) represents the predominant transcript and encodes the shorter isoform (1).