KIF3A (NM_007054) Human Untagged Clone

SKU SC115725

KIF3A (untagged)-Human kinesin family member 3A (KIF3A)

Product Data

• Type: Human Untagged Clone
• Target Symbol: KIF3A
• Synonyms: FLA10; KLP-20
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_007054 edited; GAATTCGGCACGAGGTAGTCTCTGGCCATCCTTTCTGCGCACCCGGTGTCGCTGGGCTGC; ACCCCGGGCGGGGACGTCCGCCGGGCACGGGAGGGGGCCAAGATGCCGATCAATAAATCA; GAGAAGCCAGAAAGCTGCGATAATGTGAAGGTTGTTGTTAGGTGCCGGCCCCTCAATGAG; AGAGAGAAATCAATGTGCTACAAACAGGCTGTCAGTGTGGATGAGATGAGGGGAACTATC; ACTGTACATAAGACTGATTCTTCCAATGAACCTCCAAAGACATTTACTTTTGATACTGTT; TTTGGACCAGAGAGTAAACAACTTGATGTTTATAACTTAACTGCAAGACCTATTATTGAT; TCTGTACTTGAAGGCTACAATGGGACTATTTTTGCATATGGACAAACCGGAACAGGCAAA; ACTTTTACCATGGAAGGTGTTCGAGCTATTCCTGAACTTAGAGGAATAATTCCCAATTCA; TTTGCTCACATATTTGGTCATATTGCAAAAGCGGAGGGTGATACAAGATTTTTGGTTCGA; GTGTCTTATTTGGAAATATATAATGAAGAAGTTCGTGACCTTTTGGGCAAGGATCAGACA; CAAAGGTTAGAGGTTAAAGAAAGACCTGATGTGGGAGTTTATATCAAAGATTTATCAGCT; TATGTGGTAAATAATGCTGATGATATGGATAGAATTATGACGCTAGGCCACAAAAATCGT; TCTGTTGGTGCAACTAATATGAACGAACATAGTTCCCGTTCCCATGCCATCTTTACAATT; ACTATAGAATGCAGTGAAAAAGGCATTGATGGTAACATGCATGTCAGGATGGGGAAGCTC; CATCTTGTAGATCTTGCTGGTTCAGAAAGACAGGCAAAAACTGGAGCTACTGGACAGCGC; CTAAAGGAAGCTACAAAAATCAATCTTTCACTTTCCACCCTTGGTAATGTAATTTCTGCC; TTGGTTGATGGAAAAAGCACTCATGTGCCTTATCGTAACTCTAAACTGACTCGTCTTCTT; CAGGATTCCTTAGGAGGAAATTCAAAAACCATGATGTGTGCAAATATTGGGCCAGCAGAT; TACAATTATGATGAAACTATCAGTACATTACGGTATGCCAATCGTGCTAAGAATATTAAA; AATAAAGCTAGAATTAATGAAGATCCAAAGGATGCTTTGCTGCGTCAGTTCCAGAAAGAA; ATAGAAGAACTGAAAAAGAAGCTTGAAGAAGGAGAAGAAATATCAGGCTCTGATATCAGT; GGGTCAGAGGAAGATGATGATGAAGAGGGTGAGGTTGGAGAAGATGGAGAGAAAAGGAAA; AAAAGAAGGGATCAAACAGGAAAAAAGAAAGTCTCCCCAGACAAGATGATTGAAATGCAA; GCAAAAATTGATGAGGAGAGAAAAGCACTTGAAACAAAGCTCGACATGGAAGAAGAAGAA; AGAAACAAGGCTAGAGCTGAATTAGAGAAACGGGAAAAAGATCTTCTTAAAGCCCAACAA; GAGCATCAGTCTTTGCTGGAAAAATTATCTGCCCTGGAAAAGAAGGTAATTGTTGGTGGG; GTTGACTTGTTGGCCAAAGCTGAGGAACAAGAGAAACTTCTTGAAGAATCTAACATGGAA; CTGGAAGAAAGGAGGAAAAGAGCAGAGCAACTTCGCAGAGAACTTGAGGAAAAAGAGCAA; GAACGCTTGGATATTGAAGAAAAATATACCAGTTTGCAAGAGGAAGCACAGGGAAAGACC; AAGAAGTTAAAGAAAGTTTGGACTATGCTGATGGCTGCAAAGTCAGAGATGGCTGATCTC; CAACAAGAACATCAGAGGGAAATTGAAGGCCTACTGGAGAACATTCGGCAACTTAGCCGG; GAGCTTCGACTTCAGATGCTTATTATTGATAACTTTATACCTCGGGATTATCAGGAAATG; ATTGAAAACTATGTCCATTGGAATGAAGACATAGGAGAATGGCAGCTAAAATGTGTTGCT; TATACAGGAAATAACATGAGGAAGCAAACCCCAGTACCTGATAAAAAGGAGAAAGATCCC; TTTGAGGTGGACCTTTCTCACGTGTATCTTGCCTATACTGAGGAGAGTCTGCGTCAGTCT; TTGATGAAACTAGAAAGACCACGAACTTCAAAGGGGAAAGCAAGGCCAAAGACAGGGAGA; AGAAAGCGTTCTGCAAAGCCTGAAACTGTAATTGACTCTTTACTGCAGTAAATGTTACAG; ACTTAAAGTCACAATAAAAATTAGTGATATTCTCATGCCTGGACAAAATCTTTAATTAAA; ACAGTGAAGACTTCTCTATAATTTCAATTAATGGAAGTTGTAGATCAATGAATAAGACTG; GAAATAATAATTTGCTTAAGAACTTTTAGTCTACATATATTAATATAACATTAAAATTGT; ACAACTGGTGATTGTTCAAATTGTTGTACTACTACTCTTCAATTGTACTGTGCAGGGAAG; TTGGGGGAACAGTTCATACTATAGAGAGTTACAGTTTAGATGTATGTGTAAATCGATTAG; CTATTTATCCAACTACTGTATATTTAGGTAACTAGAATTTCAAAGTAGAAAAAAAAAAAA; AAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007054 unedited; GCTCGNGATTTTGTAAACGCACTTTACTATAGGGCGGCAAAGCCATTCGGCACGAGGTAG; TCTCTGGCCATCCTTTCTGCGCACCCGGTGTCGCTGGGCTGCACCCCGGGCGGGGACGTC; CGCCGGGCACGGGAGGGGGCCAAGATGCCGATCAATAAATCAGAGAAGCCAGAAAGCTGC; GATAATGTGAAGGTTGTTGTTAGGTGCCGGCCCCTCTTTTTAGAGAGAAATCAATGTGCT; ACAAACAGGCTGTCAGTGTGGATGAGATGAGGGGAACTATCACTGTACATAAGACTGATT; CTTCCAATGAACCTCCAAAGACATTTACTTTTGATACTGTTTTTGGACCAGAGAGTAAAC; AACTTGATGTTTATAACTTAACTGCAAGACCTATTATTGATTCTGTACTTGAAGGCTACA; ATGGGACTATTTTTGCATATGGACAAACCGGAACAGGCAAAACTTTTACCATGGAAGGTG; TTCGAGCTATTCCTGAACTTAGAGGAATAATTCCCAATTCATTTGCTCACATATTTGGTC; ATATTGCAAAAGCGGAGGGTGATACAAGATTTTTGGTTCGAGTGTCTTATTTGGAAATAT; ATAATGAAGAAGTTCGTGACCTTTTGGGCAAGGATCAGACACAAAGGTTAGAGGTTAAAG; AAAGACCTGATGTGGGAGTTTATATCANAGATTTATCAGCTTATGTGGTAAATAATGCTG; ATGATATGGATAGAATTATGACGCTAGGCCACANAAATCGTTCTGTTGGTGCAACTAATA; TGAACGAACATAGTTCCCGTTCCCATGCCATCTTTACNATTACTATAGAATGCAGTGAAN; AAAGCATTGATGGTAACATGCATGTCAGGATGGGGAAAAGCTCATCTGTANATCTTGCTG; GTTCAAAAAGACAGGCAAAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007054 unedited; TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTACTTTGAAATTCT; AGTTACCTAAATATACAGTAGTTGGATAAATAGCTAATCGATTTACACATACATCTAAAC; TGTAACTCTCTATAGTATGAACTGTTCCCCCAACTTCCCTGCACAGTACAATTGAAGAGT; AGTAGTACAACAATTTGAACAATCACCAGTTGTACAATTTTAATGTTATATTAATATATG; TAGACTAAAAGTTCTTAAGCAAATTATTATTTCCAGTCTTATTCATTGATCTACAACTTC; CATTAATTGAAATTATAGAGAAGTCTTCACTGTTTTAATTAAAGATTTTGTCCAGGCATG; AGAATATCACTAATTTTTATTGTGACTTTAAGTCTGTAACATTTACTGCAGTAAAGAGTC; AATTACAGTTTCAGGCTTTGCAGAACGCTTTCTTCTCCCTGTCTTTGGCCTTGCTTTCCC; CTTTGAAGTTCGTGGTCTTTCTAGTTTCATCAAAGACTGACGCAGACTCTCCTCAGTATA; GGCAAGATACACGTGAGAAAGGTCCACCTCAAAGGGATCTTTCTCCTTTNTATCAAGTAC; TGGGGTTTGCTTCCTCATGTTATTTCCTGTATAAGCACCACATTTTAGCTGCCATTCTCC; TATGTCTTCATTCCATGGACATAGTTTTCAATCATTTCCTGATAATCCCGAGGGTATAAG; TTATCAATAATAAGCATCTGGAGTCGAAGCTCCCGGCTAGTTTGCCGATGTTCTCCAGTA; AGCCTCCATTTCCCTCTGATGTTCTTGTTGAAGATAGCCATCTCTGACTTTGCACCATCA; GATAGTCCCAACTTCCTTTACCTNCTGGCTTTCCCTGGGCTNCCTCTTGAAACTGGATAA; TTTTCTTAATATCCAGCGTTTCTGCTCTTTTCCTCAGTTTCTTGGAAGNGGCTGGCTTTT; TCCTCCTTCTCCAAGTCATGGAAAATT;
• Restriction Sites: NotI-NotI
• ACCN: NM_007054
• Insert Size: 2660 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_007054.4, NP_008985.3
• RefSeq Size: 3282 bp
• RefSeq ORF: 2100 bp
• Locus ID: 11127
• UniProt ID: Q9Y496
• Cytogenetics: 5q31.1
• Domains: kinesin
• Protein Families: Druggable Genome
• Summary: Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation. Plays a role in centriole cohesion and subdistal appendage organization and function. Regulates the formation of the subdistal appendage via recruitement of DCTN1 to the centriole. Also required for ciliary basal feet formation and microtubule anchoring to mother centriole.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (3) lacks two consecutive exons in the coding region but maintains the reading frame, compared to variant 1. The resulting isoform (3) lacks an internal segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.