POLG2 (NM_007215) Human Untagged Clone

SKU SC115638

POLG2 (untagged)-Human polymerase (DNA directed), gamma 2, accessory subunit (POLG2), nuclear gene encoding mitochondrial protein

Product Data

• Type: Human Untagged Clone
• Target Symbol: POLG2
• Synonyms: HP55; MTDPS16; MTPOLB; PEOA4; POLB; POLG-BETA; POLGB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115638 sequence for NM_007215 edited (data generated by NextGen Sequencing); ATGCGCTCTCGTGTAGCCGTCAGGGCCTGCCATAAGGTCTGCAGGTGCCTGTTGTCTGGG; TTTGGGGGTCGAGTAGATGCGGGGCAGCCGGAGCTGTTGACGGAAAGGAGTAGCCCCAAA; GGAGGGCATGTGAAGTCGCACGCGGAGCTCGAGGGGAACGGCGAGCACCCAGAAGCCCCC; GGGTCTGGAGAGGGAAGCGAGGCGCTGTTAGAGATCTGTCAGAGAAGGCATTTCCTAAGT; GGAAGCAAGCAGCAGCTTAGCCGGGATTCTCTTCTGAGTGGGTGCCACCCCGGCTTCGGA; CCCTTGGGCGTAGAGTTGCGGAAGAACCTGGCCGCAGAATGGTGGACCTCGGTGGTGGTG; TTCAGGGAGCAGGTATTCCCGGTGGACGCCCTCCACCACAAACCAGGCCCTTTGCTACCC; GGGGACAGTGCCTTCAGGTTAGTTTCTGCAGAAACTCTACGCGAAATCTTGCAAGACAAA; GAGCTGAGTAAGGAACAGCTAGTAGCATTTCTTGAGAACGTATTAAAAACTTCTGGGAAA; CTACGGGAGAACCTTCTTCACGGTGCCTTGGAACACTATGTTAATTGCCTGGATCTGGTA; AACAAGAGGCTACCTTATGGCCTTGCTCAGATTGGAGTGTGTTTTCATCCTGTTTTTGAC; ACTAAGCAGATACGAAATGGTGTTAAAAGTATTGGTGAGAAGACTGAAGCTTCGTTAGTA; TGGTTTACTCCTCCGAGAACTTCAAACCAGTGGCTTGATTTCTGGTTACGTCATCGACTC; CAGTGGTGGAGAAAGTTTGCCATGAGTCCATCTAACTTCAGCAGCAGTGACTGTCAGGAT; GAAGAAGGCCGGAAAGGAAACAAACTTTACTACAATTTTCCCTGGGGAAAGGAGTTAATA; GAAACCCTGTGGAACCTAGGAGATCACGAACTTTTACACATGTATCCTGGCAATGTGTCT; AAATTACATGGCCGAGATGGACGAAAAAATGTGGTTCCTTGTGTTCTCTCTGTAAATGGG; GACCTAGACCGAGGCATGCTGGCCTACCTCTATGATTCTTTCCAGCTGACAGAGAACTCC; TTTACAAGAAAGAAAAATCTTCATAGAAAGGTACTTAAACTTCACCCTTGTTTAGCCCCT; ATTAAGGTTGCTTTGGATGTAGGAAGAGGCCCCACATTGGAACTAAGACAGGTTTGTCAA; GGGCTATTTAATGAGTTACTAGAAAATGGGATTTCTGTGTGGCCTGGTTATTTGGAAACT; ATGCAGTCCTCATTGGAACAACTTTATTCGAAGTATGATGAAATGAGTATTCTCTTCACA; GTTTTGGTTACTGAAACTACTTTGGAGAATGGATTAATACATCTGAGAAGCAGAGACACC; ACAATGAAGGAAATGATGCATATATCCAAATTAAAAGACTTTTTGATTAAGTATATATCA; TCAGCTAAGAATGTATAG; ; Clone variation with respect to NM_007215.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007215 unedited; GGTTCAAATATTTGTATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGCTCCG; GATTCTGTTAACGGTAGTGGTGGCTTGTTGGGATCCGTTGAGTGATGGGAGAGTGTGCTC; TTTAACTTCGGAGAGAGATGCGCTCTCGTGTAGCCGTCAGGGCCTGCCATAAGGTCTGCA; GGTGCCTGTTGTCTGGGTTTGGGGGTCGAGTAGATGCGGGGCAGCCGGAGCTGTTGACGG; AAAGGAGTAGCCCCAAAGGAGGGCATGTGAAGTCGCACGCGGAGCTCGAGGGGAACGGCG; AGCACCCAGAAGCCCCCGGGTCTGGAGAGGGAAGCGAGGCGCTGTTAGAGATCTGTCAGA; GAAGGCATTTCCTAAGTGGAAGCAAGCAGCAGCTTAGCCGGGATTCTCTTCTGAGTGGGT; GCCACCCCGGCTTCGGACCCTTGGGCGTAGAGTTGCGGAAGAACCTGGCCGCAGAATGGT; GGACCTCGGTGGTGGTGTTCAGGGAGCAGGTATTCCCGGTGGACGCCCTCCACCACAAAC; CAGGCCCTTTGCTACCCGGGGACAGTGCCTTCAGGTTAGTTTCTGCAGAAACTCTACGCG; AAATCTTGCAAGACAAAGAGCTGAGTAAGGAACAGCTAGTAGCATTTCTTGAGAACGTAT; TAAAAACTTCTGGGAAACTACGGGAGAACCTTCTTCACGGTGCCTTGGAACACTATGTTA; ATTGCCTGGATCTGGNTAACAAGAGGCTACCTTATGGCCTTGCTCAGATTGGAGTGTGTT; TTCATCCTGTTTTTGACACTAAGCAGATACGAAATGGTGTTAAAAGTATTGGTGAGAAGA; CTGAAGCTTCGTTAGTATGGTTTACTCCTCCGAGAACTTCAACCAGTGGGCTNGATTCTG; GTTACGTCATCGACTCCAGTGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007215 unedited; TGTACCGCGGCCCGCATTCTAGNATCGAGTTTTTTTTTTTTTTTTTTAGAGAGAANAATT; TTATTATACAAATATAAAAATCTATACATTCTTAGCTGATGATATATACTTAATCAAAAA; GTCTTTTAATTTGGATATATGCATCATTTCCTTCATTGTGGTGTCTCTGCTTCTCAGATG; TATTAATCCATTCTCCAAAGTAGTTTCAGTAACCAAAACTGTGAAGAGAATACTCATTTC; ATCATACTTCGAATAAAGTTGTTCCAATGAGGACTGCATAGTTTCCAAATAACCAGGCCA; CACAGAAATCCCATTTTCTAGTAACTCATTAAATAGCCCTTGACAAACCTGTCTTAGTTC; CAATGTGGGGCCTCTTCCTACATCCAAAGCAACCTTAATAGGGGCTAAACAAGGGTGAAG; TTTAAGTACCTTTCTATGAAGATTTTTCTTTCTTGTAAAGGAGTTCTCTGTCAGCTGGAA; AGAATCATAGAGGTAGGCCAGCATGCCTCGGTCTAGGTCCCCATTTACAGAGAGAACACA; AGGAACCACATTTTTTCGTCCATCTCGGCCATGTAATTTAGACACATTGCCAGGATACAT; GTGTAAAAGTTCGTGATCTCCTAGGTTCCACAGGGTTTCTATTAACTCCTTTCCCCAGGG; AAAATTGTAGTAAAGTTTGTTTCCTTTCCCGCCTTCTTCATCCTGACAGTCACTGCTGCT; GAAGTTAGATGGACTCATGGCAAACTTTCTCCACCACTGGAGTCGATGACGTACCCAGAA; ATCAGCCCACTGGGTTTGAGTTCTCGGAGGAGTAAACATACTAACGAAGCTTCAGTTCTC; TCACCCATACTCTTAACACCATTTCGTATCCTGCTAGATGTCAAAACCAGATGAAAACAC; ACTCCATCTGAACCAGCCCTAAGTAGCCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_007215
• Insert Size: 1600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_007215.2, NP_009146.2
• RefSeq Size: 1595 bp
• RefSeq ORF: 1458 bp
• Locus ID: 11232
• UniProt ID: Q9UHN1
• Cytogenetics: 17q23.3
• Domains: HGTP_anticodon
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Metabolic pathways
• Summary: This gene encodes the processivity subunit of the mitochondrial DNA polymerase gamma. The encoded protein forms a heterotrimer containing one catalytic subunit and two processivity subunits. This protein enhances DNA binding and promotes processive DNA synthesis. Mutations in this gene result in autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions.[provided by RefSeq, Sep 2009]