LYPLA2 (NM_007260) Human Untagged Clone

SKU
SC115622
LYPLA2 (untagged)-Human lysophospholipase II (LYPLA2)
$450.00
In Stock*
Specifications
Product Data
Type Human Untagged Clone
Target Symbol LYPLA2
Synonyms APT-2; APT2; DJ886K2.4
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC115622 sequence for NM_007260 edited (data generated by NextGen Sequencing)
ATGTGTGGTAACACCATGTCTGTGCCCCTGCTCACCGATGCTGCCACCGTGTCTGGAGCT
GAGCGGGAAACGGCCGCGGTTATTTTTTTACATGGACTTGGAGACACAGGGCACAGCTGG
GCTGACGCCCTCTCCACCATCCGGCTCCCTCACGTCAAGTACATCTGTCCCCATGCGCCT
AGGATCCCTGTGACCCTCAACATGAAGATGGTGATGCCCTCCTGGTTTGACCTGATGGGG
CTGAGTCCAGATGCCCCAGAGGACGAGGCTGGCATCAAGAAGGCAGCAGAGAACATCAAG
GCCTTGATTGAGCATGAAATGAAGAACGGGATCCCTGCCAATCGAATCGTCCTGGGAGGC
TTTTCACAGGGCGGGGCCCTGTCCCTCTACACGGCCCTCACCTGCCCCCACCCTCTGGCT
GGCATCGTGGCGTTGAGCTGCTGGCTGCCTCTGCACCGGGCCTTCCCCCAGGCAGCTAAT
GGCAGTGCCAAGGACCTGGCCATACTCCAGTGCCATGGGGAGCTGGACCCCATGGTGCCC
GTACGGTTTGGGGCCCTGACGGCTGAGAAGCTCCGGTCTGTTGTCACACCTGCCAGGGTC
CAGTTCAAGACATACCCGGGTGTCATGCACAGCTCCTGTCCTCAGGAGATGGCAGCTGTG
AAGGAATTTCTTGAGAAGCTGCTGCCTCCTGTCTAA

Clone variation with respect to NM_007260.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_007260 unedited
GGGGGTTCANATTTTNGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGCGG
GAGAAAGAGGAGAATCGCCCAGCGGCCTCGGAAGTCCCAGGGAGTGGAGGCCCCCGCCGT
GGAGCCGTGTGGTGTATGTGTGGTAACACCATGTCTGTGCCCCTGCTCACCGATGCTGCC
ACCGTGTCTGGAGCTGAGCGGGAAACGGCCGCGGTTATTTTTTTACATGGACTTGGAGAC
ACAGGGCACAGCTGGGCTGACGCCCTCTCCACCATCCGGCTCCCTCACGTCAAGTACATC
TGTCCCCATGCGCCTAGGATCCCTGTGACCCTCAACATGAAGATGGTGATGCCCTCCTGG
TTTGACCTGATGGGGCTGAGTCCAGATGCCCCAGAGGACGAGGCTGGCATCAAGAAGGCA
GCAGAGAACATCAAGGCCTTGATTGAGCATGAAATGAAGAACGGGATCCCTGCCAATCGA
ATCGTCCTGGGAGGCTTTTCACAGGGCGGGGCCCTGTCCCTCTACACGGCCCTCACCTGC
CCCCACCCTCTGGCTGGCATCGTGGCGTTGAGCTGCTGGCTGCCTCTGCACCGGGCCTTC
CCCCAGGCAGCTAATGGCAGTGCCAAGGACCTGGCCATACTCCAGTGCCATGGGGAGCTG
GACCCCATGGTGCCCGTACGGTTTGGGGCCCTGACGGCTGAGAAGCTCCGGTCTGTTGTC
ACACCTGCCAGGGTCCAGTTCAAGACATACCCGGGTGTCATGCACAGCTCCTGTCCTCAG
GAGATGGCAGCTGTGAAGGAAATTCTTGAGAAGCTGCTGCCTCCTGTCTAACTAGTCGCT
GGCCCCAGTGCAGTACCCCAGCTCATGGGGGACTCACAAGCAAGCGTG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_007260 unedited
ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGGAAGCAATTTCTTT
AATTTTATCGGAATCCAGGACACAACAAGAAAAACACCCAAAAACCACATGGGAGACAGA
AGACGAGACACAACTCCTCCCCCACTGCCTCCCTGCTCTAGAGTGGGGACAAAGTGGGGG
TGAGACAGCTGGGGGGAGACCTGAACCTCAGTCCAGCCCTACAGGCTCCAGGCCTGCAGG
GAAGGAGGGCAACGGGGAGGCAGGGCCCAGCCCCCCAGTGTGGGGAAACAGCTGACGGAA
GGCCCCCCTCCCAAGGCTCCCCCCTTAACCAGCACTTCCGCCCCGGCCCAGGCACTTCTC
ATTTTCCCGGGGTCTCCCGGTGCCTCGGCACTCCCCCGCAAATGCCACCCCTGCCCTCCT
CGCCCCCTCACTCCTTCCTCTCTCTCACATTATCCCCCCCTCTATCTCCACTGCCTCCCC
CCCCGGCGTAGTGTCTCTACCGCCCCCCCGCCCTCCTTCCTCCCCCGGCCGCGCTCCCGC
GCCCTCCCCCCCCCGGCCGGTGCCGTCTTACTTGCTCCCTCCCTGTTCTCCCATGTCCCC
TCCCCCCTTTCCCCATCAGAGTGTCGCCTTTCTCCCCCCCTCGCCTCTACGTCCCCCCGC
CGCGCCCCCCTCGTTTTCCCCGCGTGTTCCCCCCTGTCCCCCGCCCCCCGGACGCCGCCT
CCCGCGTGTGCGCCCCCCCCGTTGTCTCAGACTCTCCCCCCTCGGCTGTACCTGGAGTCC
NGCGCCTTTCTGCGATCCAGCATCCACCCTGTCATTCTGCTCACCCTGCCCCCTTCGCCT
CCACGCCGCCACCGCTTCCTCGGCGCTCTTCCCCCTCCATCACACTGTTCCCGCACACTG
GAGCTCGGTATACTCTTTCCCCCCGCTG
Restriction Sites ECoRI-NOT
ACCN NM_007260
Insert Size 1660 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007260.2, NP_009191.1
RefSeq Size 1648 bp
RefSeq ORF 696 bp
Locus ID 11313
UniProt ID O95372
Cytogenetics 1p36.11
Domains abhydrolase_2
Protein Pathways Glycerophospholipid metabolism
Summary Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. There are alternatively spliced transcript variants described for this gene but the full length nature is not known yet. [provided by RefSeq, Jul 2008]
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Citations

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