POU6F2 (NM_007252) Human Untagged Clone

SKU SC115616

POU6F2 (untagged)-Human POU class 6 homeobox 2 (POU6F2), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: POU6F2
• Synonyms: RPF-1; WT5; WTSL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_007252, the custom clone sequence may differ by one or more nucleotides; ; ; ATGAGTGCTCTTCTTCAGGATCCAATGATAGCTGGACAAGTCAGTAAGCCCTTGCTGTCAGTGCGGAGTG; AAATGAATGCGGAGTTGAGAGGTGAGGACAAGGCTGCTACTTCAGACAGCGAGCTGAATGAGCCCCTGCT; TGCGCCTGTGGAATCAAATGACAGCGAGGACACTCCCAGCAAGCTCTTCGGGGCTAGAGGAAACCCAGCA; TTATCAGACCCAGGCACTCCTGACCAACACCAGGCCAGTCAGACCCACCCCCCATTTCCAGTTGGGCCAC; AGCCACTTCTGACGGCACAGCAGTTAGCTTCTGCTGTGGCCGGCGTGATGCCGGGAGGCCCCCCAGCCCT; CAACCAGCCAATCCTCATTCCCTTCAACATGGCGGGACAGCTAGGAGGCCAGCAAGGACTGGTTCTCACA; CTGCCAACAGCGAATCTCACCAACATCCAAGGGCTGGTGGCAGCAGCTGCAGCCGGAGGCATTATGACTC; TGCCACTGCAAAATCTACAAGCTACCTCATCCCTGAACTCCCAGCTCCAGCAGCTCCAGCTCCAGCTCCA; GCAGCAGCAGCAGCAGCAGCAGCAGCAGCCTCCCCCGTCAACCAACCAGCACCCGCAACCAGCCCCACAG; GCGCCCTCGCAGTCCCAGCAGCAGCCGCTGCAGCCCACCCCACCCCAGCAGCCACCACCCGCCTCTCAGC; AGCCGCCAGCTCCTACATCTCAGCTGCAACAGGCGCCTCAGCCCCAGCAGCACCAACCCCACTCCCACTC; CCAGAACCAGAACCAACCATCTCCAACCCAGCAGAGCTCCAGCCCCCCGCAGAAACCTAGTCAGTCTCCA; GGACATGGCCTGCCTTCACCGCTCACGCCACCCAATCCTCTACAGCTGGTTAATAATCCACTAGCAAGTC; AGGCTGCAGCGGCTGCAGCAGCCATGAGCTCCATAGCAAGCTCACAGGCCTTTGGCAATGCCCTCTCCAG; TCTTCAGGGGGTCACAGGTCAACTAGTTACTAATGCACAAGGACAGATTATCGGGACCATTCCACTGATG; CCTAATCCAGGGCCATCGAGCCAAGCAGCAAGCGGCACTCAGGGCTTGCAAGTGCAGCCAATCACCCCCC; AGCTCCTCACAAACGCCCAGGGCCAGATCATCGCCACAGTCATTGGGAACCAGATCCTGCCCGTGATCAA; CACCCAGGGCATCACGCTGTCACCCATCAAGCCCGGCCAGCAGCTCCACCAACCCTCCCAGACGTCAGTG; GGTCAAGCAGCCTCCCAAGGCAACCTTCTGCACCTGGCTCACAGCCAAGCATCCATGTCTCAAAGTCCCG; TCCGGCAGGCTTCCTCTTCTTCCTCCTCATCCTCCTCTTCTTCAGCTTTGAGCGTGGGCCAGTTAGTCAG; CAATCCTCAAACGGCAGCGGGTGAGGTGGATGGGGTTAATCTGGAGGAGATCCGAGAATTTGCCAAAGCT; TTTAAAATCCGGCGCCTGTCCCTTGGCCTGACCCAGACTCAGGTGGGACAGGCTCTCAGTGCTACAGAGG; GCCCCGCGTACAGCCAGTCGGCCATCTGCAGACACACCATCCTGAGAAGCCACTTTTTCCTACCACAGGA; AGCCCAAGAGAACACTATAGCTAGCAGTCTGACAGCCAAACTGAACCCTGGCCTTTTGTATCCTGCCAGG; TTTGAAAAGCTGGACATCACCCCTAAAAGTGCCCAGAAGATCAAGCCGGTGCTTGAGCGGTGGATGGCTG; AGGCTGAGGCCCGCCATCGAGCAGGTATGCAGAACCTGACCGAGTTTATCGGGAGTGAACCATCCAAAAA; GCGCAAGCGGCGCACCTCCTTCACACCCCAGGCCCTTGAGATCCTCAATGCCCACTTTGAGAAGAACACA; CACCCTTCTGGGCAGGAAATGACCGAAATTGCTGAGAAGCTGAACTATGACCGAGAAGTAGTTAGAGTTT; GGTTCTGCAATAAGAGGCAAGCCCTGAAGAACACAATTAAACGCTTAAAACAGCACGAGCCGGCCACGGC; AGTCCCTTTGGAGCCCTTAACAGACTCTCTGGAAGAAAACTCCTAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007252 unedited; GGGGGTTCAAATTTTGTAATACGACTCATATAGGCGGCCGCGAATTCGGCACGAGGATGG; ATTGCGATCTCTCAGCGCAGCAGGACACGGGGACCATGCAAGCTGTAATTGGTCAGGCAT; GAACTCTCTTGGGTTGTGTTACCCTGTTTGCTGTTTACTGTCAAGATGCTGAAAGAATGT; TCTTATAATGATCCAAGAGGAAGTGGCAAATGAGTGCTCTTCTTCAGGATCCAATGATAG; CTGGACAAGTCAGTAAGCCCTTGCTGTCAGTGCGGAGTGAAATGAATGCGGAGTTGAGAG; GTGAGGACAAGGCTGCTACTTCAGACAGCGAGCTGAATGAGCCCCTGCTTGCGCCTGTGG; AATCAAATGACAGCGAGGACACTCCCAGCAAGCTCTTCGGGGCTAGAGGAAACCCAGCAT; TATCAGACCCAGGCACTCCTGACCAACACCAGGCCAGTCAGACCCACCCCCCATTTCCAG; TTGGGCCACAGCCACTTCTGACGGCACAGCAGTTAGCTTCTGCTGTGGCCGGCGTGATGC; CGGGAGGCCCCCCAGCCCTCAACCAGCCAATCCTCATTCCCTTCAACATGGCGGGACAGC; TAGGAGGCCAGCAAGGACTGGTTCTCACACTGCCAACAGCGAATCTCACCAACATCCAAG; GGCTGGTGGCAGCAGCTGCAGCCGGAGGCATTATGACTCTGCCACTGCAAAATCTACAAG; CTACCTCATCCCTGAACTCCCAGCTCCAGCAGCTTCAGCTTCAGCTCCAGCAGCAGCAGC; AGCAGCAGCAGCAGCAGCCTTCCCCGTCAACCAACCAGCACCGNNAACCAGCCCCACANG; CCCCCCTCGCAGTCCCAGCAGCACCCGCTTGCAGCCCACCCCACCCCAGCAGNCACCACC; CGNCTCTCAGCAGCCGNCAGCTTCTACTTNTAGCTGCAACAGGCGC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007252 unedited; CTATGGACCGCGGCCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCTTAAAATGT; GTTCACATTTTCATAATATACAGAAAAAATACGTGGTTATTTGGTTTGCTTTAAGTCCAT; TAAAAAACTGGCTTTGGGGGAGACAGGTGGGGGAAGGGAACTATCCTACCACACACACCT; TCAGTGTGGTGTATTCCTTGTTTAAAAATTTTCTAAAAATTTTCTTCTTTCCTTTTTGGA; AAACCAACGGACATGTAGAAACCATTCTGTCCACTTGGTAGTTTTCTTAGTCATTTACTT; ACAAGGGTGATGACGACTGGGGCTATTTTTAAATTAAATTAAATTTTGTTGTTGTTGTTG; TTGTGGAGTCCCAAGGGTGGAGTTTAGTTTCTGTGAATTTTGCTTCTGATTATGGGTGGG; CATCTCTTTAGGAGTTTTCTTCCAGAGAGTCTGTTAAGGGCTCCAAAGGGACTGCCGTGG; CCGGCTCGTGCTGTTTTAAGCGTTTAATTGTGTTCTTCAGGGCTTGCCTCTTATTGCAGA; ACCAAACTCTAACTACTTCTCGGTCATAGTTCAGCTTCTCAGCAATTTCGGTCATTTCCT; GCCCAGAAGGGTGTGTGTTCTTCTCAAAGTGGGCATTGAGGATCTCAAGGGCCTGGGGTG; TGAAGGAGGTGCGCCGCTTGCGCTTTTTGGATGGTTCACTCCCGATAAACTCGGTCAGGT; TCTGCATACCTGCTCGATGGCGGGCCTCAGCCTCAGCCATCCACCGCTCAAGCACCGGCT; TGATCTTCTGGGCACTTTTAGGGGTGATGTCCAGCTTTTCAACCTGCANATGGCCGACTG; GCTGTACGCGGGGCCCTCTGTAGCACTGAGAGCCCTGTCCCACTGATCTGGGTCANGCCA; GGGGACAGCGCCGNATTTTAAAACTTTNGCAATTTCTCGATCTCTCAGATAACCCCTCCA; CTC;
• Restriction Sites: NotI-NotI
• ACCN: NM_007252
• Insert Size: 2480 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_007252.2, NP_009183.3
• RefSeq Size: 2223 bp
• RefSeq ORF: 2076 bp
• Locus ID: 11281
• UniProt ID: P78424
• Cytogenetics: 7p14.1
• Domains: homeobox, POU
• Protein Families: Transcription Factors
• Summary: This gene encodes a member of the POU protein family characterized by the presence of a bipartite DNA binding domain, consisting of a POU-specific domain and a homeodomain, separated by a variable polylinker. The DNA binding domain may bind to DNA as monomers or as homo- and/or heterodimers, in a sequence-specific manner. The POU family members are transcriptional regulators, many of which are known to control cell type-specific differentiation pathways. This gene is a tumor suppressor involved in Wilms tumor (WT) predisposition. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Oct 2009]; Transcript Variant: This variant (1) is the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.