DRP1 (DNM1L) (NM_012062) Human Untagged Clone

CAT#: SC115574

DNM1L (untagged)-Human dynamin 1-like (DNM1L), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DRP1
• Synonyms: DLP1; DRP1; DVLP; DYMPLE; EMPF; EMPF1; HDYNIV; OPA5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_012062 edited
  GGCGAACTGTGGGCCCCGGCCCCATTCATTGCCGTGGCCGGCGGGCACTGGGGCCCCGTG
  TTTTCAGAGTCATGGAGGCGCTAATTCCTGTCATAAACAAGCTCCAGGACGTCTTCAACA
  CGGTGGGCGCCGACATCATCCAGCTGCCTCAAATCGTCGTAGTGGGAACGCAGAGCAGCG
  GAAAGAGCTCAGTGCTAGAAAGCCTGGTGGGGAGGGACCTGCTTCCCAGAGGTACTGGAA
  TTGTCACCCGGAGACCTCTCATTCTGCAACTGGTCCATGTTTCACAAGAAGATAAACGGA
  AAACAACAGGAGAAGAAAATGGGGTGGAAGCAGAAGAATGGGGTAAATTTCTTCACACCA
  AAAATAAGCTTTACACGGATTTTGATGAAATTCGACAAGAAATTGAAAATGAAACAGAAA
  GAATTTCAGGAAATAATAAGGGAGTAAGCCCTGAACCAATTCATCTTAAGATTTTTTCAC
  CCAACGTTGTCAATTTGACACTTGTGGATTTGCCAGGAATGACCAAGGTGCCTGTAGGTG
  ATCAACCTAAGGATATTGAGCTTCAAATCAGAGAGCTCATTCTTCGGTTCATCAGTAATC
  CTAATTCCATTATCCTCGCTGTCACTGCTGCTAATACAGATATGGCAACATCAGAGGCAC
  TTAAAATTTCAAGAGAGGTAGATCCAGATGGTCGCAGAACCCTAGCTGTAATCACTAAAC
  TTGATCTCATGGATGCGGGTACTGATGCCATGGATGTATTGATGGGAAGGGTTATTCCAG
  TCAAACTTGGAATAATTGGAGTAGTTAACAGGAGCCAGCTAGATATTAACAACAAGAAGA
  GTGTAACTGATTCAATCCGTGATGAGTATGCTTTTCTTCAAAAGAAATATCCATCTCTGG
  CCAATAGAAATGGAACAAAGTATCTTGCTAGGACTCTAAACAGGTTACTGATGCATCACA
  TCAGAGATTGTTTACCAGAGTTGAAAACAAGAATAAATGTTCTAGCTGCTCAGTATCAGT
  CTCTTCTAAATAGCTACGGTGAACCCGTGGATGATAAAAGTGCTACTTTACTCCAACTTA
  TTACCAAATTTGCCACAGAATATTGTAACACTATTGAAGGAACTGCAAAATATATTGAAA
  CTTCGGAGCTATGCGGTGGTGCTAGAATTTGTTATATTTTCCATGAGACTTTTGGGCGAA
  CCTTAGAATCTGTTGATCCACTTGGTGGCCTTAACACTATTGACATTTTGACTGCCATTA
  GAAATGCTACTGGTCCTCGTCCTGCTTTATTTGTGCCTGAGGTTTCATTTGAGTTACTGG
  TGAAGCGGCAAATCAAACGTCTAGAAGAGCCCAGCCTCCGCTGTGTGGAACTGGTTCATG
  AGGAAATGCAAAGGATCATTCAGCACTGTAGCAATTACAGTACACAGGAATTGTTACGAT
  TTCCTAAACTTCATGATGCCATAGTTGAAGTGGTGACTTGTCTTCTTCGTAAAAGGTTGC
  CTGTTACAAATGAAATGGTCCATAACTTAGTGGCAATTGAACTGGCTTATATCAACACAA
  AACATCCAGACTTTGCTGATGCTTGTGGGCTAATGAACAATAATATAGAGGAACAAAGGA
  GAAACAGGCTAGCCAGAGAATTACCTTCAGCTGTATCACGAGACAAGTCTTCTAAAGTTC
  CAAGTGCTTTGGCACCTGCCTCCCAGGAGCCCTCCCCCGCTGCTTCTGCTGAGGCTGATG
  GCAAGTTAATTCAGGACAGCAGAAGAGAAACTAAAAATGTTGCATCTGGAGGTGGTGGGG
  TTGGAGATGGTGTTCAAGAACCAACCACAGGCAACTGGAGAGGAATGCTGAAAACTTCAA
  AAGCTGAAGAGTTATTAGCAGAAGAAAAATCAAAACCCATTCCAATTATGCCAGCCAGTC
  CACAAAAAGGTCATGCCGTGAACCTGCTAGATGTGCCAGTTCCTGTTGCACGAAAACTAT
  CTGCTCGGGAACAGCGAGATTGTGAGGTTATTGAACGACTCATTAAATCATATTTTCTCA
  TTGTCAGAAAGAATATTCAAGACAGTGTGCCAAAGGCAGTAATGCATTTTTTGGTTAATC
  ATGTGAAAGACACTCTTCAGAGTGAGCTAGTAGGCCAGCTGTATAAATCATCCTTATTGG
  ATGATCTTCTGACAGAATCTGAGGACATGGCACAGCGCAGGAAAGAAGCAGCTGATATGC
  TAAAGGCATTACAAGGAGCCAGTCAAATTATTGCTGAAATCCGGGAGACTCATCTTTGGT
  GAAGAGAACTATGTAATACTGAGACTTTGTTGACTCAAAACTTGCTAGTTACTGCCTACC
  TGAGTAGAATCTTATTTATGAACTCCTGTGTATTGCAATGGTATGAATCTGCTCATGTGG
  AGACTGGCTATAAACTGAAAAGTGTATTCCAAATTGCAGAACACATCACACATTTAATCC
  AAATAATAAATGGCTGTTTCTAAAGTTTCCCAGTATATATAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012062 unedited
  NNNAACGTTCAAATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGG
  GCGAACTGTGGGCCCCGGCCCCATTCATTGCCGTGGCCGGCGGGCACTGGGGCCCCGTGT
  TTTCAGAGTCATGGAGGCGCTAATTCCTGTCATAAACAAGCTCCAGGACGTCTTCAACAC
  GGTGGGCGCCGACATCATCCAGCTGCCTCAAATCGTCGTAGTGGGAACGCAGAGCAGCGG
  AAAGAGCTCAGTGCTAGAAAGCCTGGTGGGGAGGGACCTGCTTCCCAGAGGTACTGGAAT
  TGTCACCCGGAGACCTCTCATTCTGCAACTGGTCCATGTTTCACAAGAAGATAAACGGAA
  AACAACAGGAGAAGAAAATGGGGTGGAAGCAGAAGAATGGGGTAAATTTCTTCACACCAA
  AAATAAGCTTTACACGGATTTTGATGAAATTCGACAAGAAATTGAAAATGAAACAGAAAG
  AATTTCAGGAAATAATAAGGGAGTAAGCCCTGAACCAATTCATCTTAAGATTTTTTCACC
  CAACGTTGTCAATTTGACACTTGTGGATTTGCCAGGAATGACCAAGGTGCCTGTAGGTGA
  TCAACCTAAGGATATTGAGCTTCAAATCAGAGAGCTCATTCTTCGGTTCATCAGTAATCC
  TAATTCCATTATCCTCGCTGTCACTGCTGCTAATACAGATATGGCAACATCAGAGGCACT
  TAAAATTTCAAGAGAGGTAGATCCAGATGGTCGCAGAACCCTAGCTGTAATCACTAAACT
  TGATCTCATGGATGCGGGTACTGATGCCATGGATGTATTGATGGGGAAGGGTTATTCCAG
  TCAAACTTGGAATAATTGGNAGTAGTTAACAGGNAGCCAGCTAGATATTAACAACAGNAA
  AAGAGTGTACTGANTNCAATCCCGGGATGAGTATGGCTTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012062 unedited
  ATGGCCGCGGCCGCATCTAGGGTCGAGTTTTTTTTTTTTTTTTTATATATACTGGNGAAA
  CTTTAGAAACAGCCATTTATTATTTGGATTAAATGTGTGATGTGTTCTGCAATTTGGAAT
  ACACTTTTCAGTTTATAGCCAGTCTCCACATGAGCAGATTCATACCATTGCAATACACAG
  GAGTTCATAAATAAGATTCTACTCAGGTAGGCAGTAACTAGCAAGTTTTGAGTCAACAAA
  GTCTCAGTATTACATAGTTCTCTTCACCAAAGATGAGTCTCCCGGATTTCAGCAATAATT
  TGACTGGCTCCTTGTAATGCCTTTAGCATATCAGCTGCTTCTTTCCTGCGCTGTGCCATG
  TCCTCAGATTCTGTCAGAAGATCATCCAATAAGGATGATTTATACAGCTGGCCTACTAGC
  TCACTCTGAAGAGTGTCTTTCACATGATTAACCAAAAAATGCATTACTGCCTTTGGCACA
  CTGTCTTGAATATTCTTTCTGACAATGAGAAAATATGATTTAATGAGTCGTTCAATAACC
  TCACAATCTCGCTGTTCCCGAGCAGATAGTTTTCGTGCAACAGGAACTGGCACATCTAGC
  AGGTTCACGGCATGACCTTTTTGTGGACTGGCTGGCATAATTGGAATGGGTTTTGATTTT
  TCTTCTGCTAATAACTCTTCAGCTTTTGGAGTTTTCAGCATTCCTCCTCAGNTGCCTGTG
  GGNTGGTTCTTGGACACCATCTCCAACCCCACCACCTCCAGATGCACATTTTTAGCTTCT
  CTCCTGCTGTCCTGAATTACCTTGCATCAGCCTCAGCCAACCACCGGGGGGGGGCTCCCG
  CAAGCAGGTGGCCCACCTTGGGACTTAAAAGACCGGCCCGGACCAGCTGAGGCATTTTGG
  CCCGCCGGCCCCCTGGTCTTTATCATGGCCACACCCCAGCTTCCCGCCGGGCGTCTGGCC
  CGCCAGCCCCCCTTCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012062
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012062.2, NP_036192.1
• RefSeq Size: 3293 bp
• RefSeq ORF: 2211 bp
• Locus ID: 10059
• UniProt ID: O00429
• Cytogenetics: 12p11.21
• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis
• Gene Summary: This gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013]
  Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.