PLD3 (NM_001031696) Human Untagged Clone

SKU SC115432

PLD3 (untagged)-Human phospholipase D family, member 3 (PLD3), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: PLD3
• Synonyms: AD19; HU-K4; HUK4; SCA46
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001031696 edited; CTGGAAGGTGCGTGTGGGGCTGGGTCTCGGAGTGGGAGACGTGGAGTGCAGGTAATGCAT; GTCCATGGTACACAAATTCACAAGGTTTGTAAATGAGAAAAGACGTGAGGTTCCTTTTGT; TCTTTACCTGTGGCCTCCCTGCCCTACACGGGGACTCTAGGGTGGAATGTAGCAAAGCCC; ATCCACCAGCCATGTACTACCCCCCAACCCGGCCAGGCTGGAGCGACCGTGTCTGGGGAG; CCGAGCCCCGCTTCTCGCTGCGGTGAGCCCGGACTGGGGCACGCACTGCGCAGACTCCCC; GCTGCAGTGGGCGGAGCTCCCACAGGCCCCGCCCCCTCCTCCCACCCTCGTTCAGCCTGT; CCAGACAGAAGCTGGGGCCCAGCGGAGGTAGCAGCAGACGCCTGAGAGCGAGGCCGAGGC; CCCTCAGGGTTTGGAGACCCTGACACACCCACCTTCTCACCTGGGCTCTGCGTATCCCCC; AGCCTTGAGGGAAGATGAAGCCTAAACTGATGTACCAGGAGCTGAAGGTGCCTGCAGAGG; AGCCCGCCAATGAGCTGCCCATGAATGAGATTGAGGCGTGGAAGGCTGCGGAAAAGAAAG; CCCGCTGGGTCCTGCTGGTCCTCATTCTGGCGGTTGTGGGCTTCGGAGCCCTGATGACTC; AGCTGTTTCTATGGGAATACGGCGACTTGCATCTCTTTGGGCCCAACCAGCGCCCAGCCC; CCTGCTATGACCCTTGCGAAGCAGTGCTGGTGGAAAGCATTCCTGAGGGCCTGGACTTCC; CCAATGCCTCCACGGGGAACCCTTCCACCAGCCAGGCCTGGCTGGGCCTGCTCGCCGGTG; CGCACAGCAGCCTGGACATCGCCTCCTTCTACTGGACCCTCACCAACAATGACACCCACA; CGCAGGAGCCCTCTGCCCAGCAGGGTGAGGAGGTCCTCCGGCAGCTGCAGACCCTGGCAC; CAAAGGGCGTGAACGTCCGCATCGCTGTGAGCAAGCCCAGCGGGCCCCAGCCACAGGCGG; ACCTGCAGGCTCTGCTGCAGAGCGGTGCCCAGGTCCGCATGGTGGACATGCAGAAGCTGA; CCCATGGCGTCCTGCATACCAAGTTCTGGGTGGTGGACCAGACCCACTTCTACCTGGGCA; GTGCCAACATGGACTGGCGTTCACTGACCCAGGTCAAGGAGCTGGGCGTGGTCATGTACA; ACTGCAGCTGCCTGGCTCGAGACCTGACCAAGATCTTTGAGGCCTACTGGTTCCTGGGCC; AGGCAGGCAGCTCCATCCCATCAACTTGGCCCCGGTTCTATGACACCCGCTACAACCAAG; AGACACCAATGGAGATCTGCCTCAATGGAACCCCTGCTCTGGCCTACCTGGCGAGTGCGC; CCCCACCCCTGTGTCCAAGTGGCCGCACTCCAGACCTGAAGGCTCTACTCAACGTGGTGG; ACAATGCCCGGAGTTTCATCTACGTCGCTGTCATGAACTACCTGCCCACTCTGGAGTTCT; CCCACCCTCACAGGTTCTGGCCTGCCATTGACGATGGGCTGCGGCGGGCCACCTACGAGC; GTGGCGTCAAGGTGCGCCTGCTCATCAGCTGCTGGGGACACTCGGAGCCATCCATGCGGG; CCTTCCTGCTCTCTCTGGCTGCCCTGCGTGACAACCATACCCACTCTGACATCCAGGTGA; AACTCTTTGTGGTCCCCGCGGATGAGGCCCAGGCTCGAATCCCATATGCCCGTGTCAACC; ACAACAAGTACATGGTGACTGAACGCGCCACCTACATCGGAACCTCCAACTGGTCTGGCA; ACTACTTCACGGAGACGGCGGGCACCTCGCTGCTGGTGACGCAGAATGGGAGGGGCGGCC; TGCGGAGCCAGCTGGAGGCCATTTTCCTGAGGGACTGGGACTCCCCTTACAGCCATGACC; TTGACACCTCAGCTGACAGCGTGGGCAACGCCTGCCGCCTGCTCTGAGGCCCGATCCAGT; GGGCAGGCCAAGGCCTGCTGGGCCCCCGCGGACCCAGGTGCTCTGGGTCACGGTCCCTGT; CCCCGCACCCCCGCTTCTGTCTGCCCCATTGTGGCTCCTCAGGCTCTCTCCCCTGCTCTC; CCACCTCTACCTCCACCCCCACCGGCCTGACGCTGTGGCCCCGGGACCCAGCAGAGCTGG; GGGAGGGATCAGCCCCCAAAGAAATGGGGGTGCATGCTGGGCCTGGCCCCCTGGCCCACC; CCCACTTTCCAGGGCAAAAAGGGCCCAGGGTTATAATAAGTAAATAACTTGTCTGTACAA; AAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001031696 unedited; CATATTTGTATACGACTCACTATAGGGCGGCCCGCGAATTCGCACGAGGCTGGAAGGTGC; GTGTGGGGCTGGGTCTCGGAGTGGGAGACGTGGAGTGCAGGTAATGCATGTCCATGGTAC; ACAAATTCACAAGGTTTGTAAATGAGAAAAGACGTGAGGTTCCTTTTGTTCTTTACCTGT; GGCCTCCCTGCCCTACACGGGGACTCTAGGGTGGAATGTAGCAAAGCCCATCCACCAGCC; ATGTACTACCCCCCAACCCGGCCAGGCTGGAGCGACCGTGTCTGGGGAGCCGAGCCCCGC; TTCTCGCTGCGGTGAGCCCGGACTGGGGCACGCACTGCGCAGACTCCCCGCTGCAGTGGG; CGGAGCTCCCACAGGCCCCGCCCCCTCCTCCCACCCTCGTTCAGCCTGTCCAGACAGAAG; CTGGGGCCCAGCGGAGGTAGCAGCAGACGCCTGAGAGCGAGGCCGAGGCCCCTCAGGGTT; TGGAGACCCTGACACACCCACCTTCTCACCTGGGCTCTGCGTATCCCCCAGCCTTGAGGG; AAGATGAAGCCTAAACTGATGTACCAGGAGCTGAAGGTGCCTGCAGAGGAGCCCGCCAAT; GAGCTGCCCATGAATGAGATTGAGGCGTGGAAGGCTGCGGAAAAGAAGCCCGCTGGGTCC; TGCTGGTCCTCATTCTGGCGGTTGTGGGCTTCGGAGCCCTGATGACTCAGCTGTTTCTAT; GGGAATACGGCGACTTGCATCTCTTTGGGCCCACCAGCGCCCAGCCCCCTGCTATGACCC; TTGCGAAGCAGTGCTGGTGGAAAGCATTCCTGAGGGCCTGGACTTCCCCATGCCTCCACG; GGAAACCTTCCCCCCCCAGGCCTGCCTGGCCTGCTCNNCGTGCCCCAGAAGCCTGNACAT; CGCTCCTTTATTGAACCTCACCACATGACACCACACCCGNAGCCTCTGCCCACCAGCGAA; GGAGGCCTCGGCACTTCAN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001031696 unedited; GCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTACAGACAAGTTATTTAC; TTATTATAACCCTGGGCCCTTTTTGCCCTGGAAAGTGGGGGTGGGCCAGGGGGCCAGGCC; CAGCATGCACCCCCATTTCTTTGGGGGCTGATCCCTCCCCCAGCTCTGCTGGGTCCCGGG; GCCACAGCGTCAGGCCGGTGGGGGTGGAGGTAGAGGTGGGAGAGCAGGGGAGAGAGCCTG; AGGAGCCACAATGGGGCAGACAGAAGCGGGGGTGCGGGGACAGGGACCGTGACCCAGAGC; ACCTGGGTCCGCGGGGGCCCAGCAGGCCTTGGCCTGCCCACTGGATCGGGCCTCAGAGCA; GGCGGCAGGCGTTGCCCACGCTGTCAGCTGAGGTGTCAAGGTCATGGCTGTAAGGGGAGT; CCCAGTCCCTCAGGAAAATGGCCTCCAGCTGGCTCCGCAGGCCGCCCCTCCCATTCTGCG; TCACCAGCAGCGAGGTGCCCGCCGTCTCCGTGAAGTAGTTGCCAGACCAGTTGGAGGTTC; CGATGTAGGTGGCGCGTTCAGTCACCATGTACTTGTTGTGGTTGACACGGGCATATGGGA; TTCGAGCCTGGGCCTCATCCGCGGGGACCACAAAGAGTTTCACCTGGATGTCAGAGTGGG; TATGGGTTGTCACGCAGGGCAGCCANAGAGAGCAGGAAAGCCCGCATGGATGGCNTCCGA; GTGTCCCCAGCAGCTGATGAGCACGCGCACCTTGACGCCAGCTCGTTAGGTGGCCCGNCG; CAGNCCATCGTCAATGGCAGGCCAGACCTGGGGAGGGTGGGGAGAACTNCANAATGGGCC; AGGTAGTTCATGACAGCGACGTAAATGAAACTCCGGGCATTGCCCACCACGTGATAAGAA; CCTCAGGGTCTGGATGCGGGCACTGAACCAGGGGGGGGGGGGCGCCCTCGCCAGGAAGCC; AA;
• Restriction Sites: NotI-NotI
• ACCN: NM_001031696
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001031696.1, NP_001026866.1
• RefSeq Size: 1954 bp
• RefSeq ORF: 1473 bp
• Locus ID: 23646
• UniProt ID: Q8IV08
• Cytogenetics: 19q13.2
• Protein Families: Transmembrane
• Summary: This gene encodes a member of the phospholipase D (PLD) family of enzymes that catalyze the hydrolysis of membrane phospholipids. The encoded protein is a single-pass type II membrane protein and contains two PLD phosphodiesterase domains. This protein influences processing of amyloid-beta precursor protein. Mutations in this gene are associated with Alzheimer disease risk. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Apr 2014]; Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Variants 1, 2 and 3 encode the same protein.