DNAJB5 (NM_012266) Human Untagged Clone

SKU SC115430

DNAJB5 (untagged)-Human DnaJ (Hsp40) homolog, subfamily B, member 5 (DNAJB5), transcript variant 3

Product Data

• Type: Human Untagged Clone
• Target Symbol: DNAJB5
• Synonyms: Hsc40
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115430 sequence for NM_012266 edited (data generated by NextGen Sequencing); ATGGGAAAAGATTATTACAAGATTCTTGGGATCCCATCGGGGGCCAACGAGGATGAGATC; AAGAAAGCCTACCGGAAGATGGCCTTGAAGTACCACCCAGACAAGAATAAAGAACCCAAC; GCTGAGGAGAAGTTTAAGGAGATTGCAGAGGCCTATGATGTGCTAAGTGACCCCAAGAAA; CGGGGCCTGTATGACCAGTATGGGGAGGAAGGCCTGAAGACCGGCGGTGGCACATCAGGT; GGCTCCAGTGGCTCCTTTCACTACACCTTTCATGGGGACCCCCATGCCACCTTTGCCTCC; TTCTTTGGTGGCTCCAACCCCTTCGATATCTTCTTTGCCAGCAGCCGCTCCACTCGGCCC; TTCAGTGGCTTTGACCCAGATGACATGGATGTGGATGAAGATGAGGACCCATTTGGCGCT; TTCGGCCGTTTTGGCTTCAATGGGCTGAGTAGGGGTCCAAGGCGAGCCCCAGAACCACTG; TACCCTCGGCGCAAGGTGCAGGACCCCCCAGTGGTGCACGAGCTGCGGGTGTCCCTGGAG; GAGATCTACCATGGCTCCACCAAGCGCATGAAGATCACAAGGCGTCGCCTCAACCCTGAT; GGGCGAACTGTGCGCACCGAGGACAAGATCCTGCACATAGTCATCAAGCGTGGCTGGAAG; GAAGGCACCAAGATCACCTTCCCCAAAGAAGGCGACGCCACACCTGACAACATCCCTGCT; GACATCGTCTTTGTGCTCAAAGACAAGCCCCATGCACACTTCCGCCGAGATGGCACCAAC; GTGCTCTACAGTGCCCTGATCAGCCTCAAGGAGGCGCTGTGTGGCTGCACTGTGAACATT; CCCACTATCGACGGCCGAGTGATCCCTTTGCCCTGCAATGATGTCATCAAGCCAGGCACC; GTGAAGAGACTCCGTGGGGAGGGCCTTCCCTTCCCCAAAGTGCCAACTCAGCGAGGAGAC; CTCATTGTTGAGTTCAAAGTTCGCTTCCCAGACAGATTAACACCACAGACAAGACAGATC; CTTAAGCAGCACCTACCCTGTTCCTAG; ; Clone variation with respect to NM_012266.5;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012266 unedited; GCCGCGGAATTCGCACGAGGAGGAGGCAGATCCTGAAAGAGCTTGCCCTGTGTCTGCGGA; GGCAAGAGATGTATGGGCCTAGAGCAGCTAGCAGAAACAAGGAGACCAGTGCTGGTCCAG; TGGCTGTGATGGGAAAAGATTATTACAAGATTCTTGGGATCCCATCGGGGGCCAACGAGG; ATGAGATCAAGAAAGCCTACCGGAAGATGGCCTTGAAGTACCACCCAGACAAGAATAAAG; AACCCAACGCTGAGGAGAAGTTTAAGGAGATTGCAGAGGCCTATGATGTGCTAAGTGACC; CCAAGAAACGGGGCCTGTATGACCAGTATGGGGAGGAAGGCCTGAAGACCGGCGGTGGCA; CATCAGGTGGCTCCAGTGGCTCCTTTCACTACACCTTTCATGGGGACCCCCATGCCACCT; TTGCCTCCTTCTTTGGTGGCTCCAACCCCTTCGATATCTTCTTTGCCAGCAGCCGCTCCA; CTCGGCCCTTCAGTGGCTTTGACCCAGATGACATGGATGTGGATGAAGATGAGGACCCAT; TTGGCGCTTTCGGCCGTTTTGGCTTCAATGGGCTGAGTAGGGGTCCAAGGCGAGCCCCAG; AACCACTGTACCCTCGGCGCAAGGTGCAGGACCCCCCAGTGGTGCACGAGCTGCGGGGTG; TCCCTGGAGGAGATCTACCATGGCTCCACCAAGCGCATGAAGATCCAANGGCGTCGCCTC; AACCCTGATGGGCGAACTGTGCGCACCGAGGGACAGATCCTGCACATAGTCTNCAAGCGT; GGCTGGAGGAANGCACCAAGATCACCTTTCCCAAGAAGCGACGCCACACTGACACATCCT; GCTGACTCGTCTTGTGCTCAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012266 unedited; NTTTTAGCTATGGACCGGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTTTT; TTTTTCCATATTTTTAAAATACTTTATTTTTTACATAATACTGTCATTACAAAAAATACA; AAAAAACTACTATAAAAACATTCGGGGGTTGTCAAAGTGAGAAAACCTAAAGACCCCACC; CCAGGATCTGGCTGAAGCAGTCTTCCCCCAGCTTCTTCACTATGACCTTTATACAACTAT; GGGGGTGGGGGGGGATCACACAGGCATAAAAGGGCTGGAAATTCCCCACACAGCCTCCAA; GGGTAAAAAATGAGTAGCTTCACATATCACAAAAGTGGGATTTGGAAGTTTGGGGGTGGC; TAGGCCCTGAGTTCAAAGGTGTGGGGAAAAACCTGTGACCCTGAATCTCTTGGGGGGGAA; TAGCTGCCACCTGACCCCAAAGCCCTTTCCCTTCCTGATGAAGGCTGGTAAATGGGCCCT; GTCCCCCACCTCTTAGCCTTAATACCTCAGGCCCCATTCCCTGCCCTCCACCCTTGAACA; CTCCTGAGAGCAGCAGGGAGGACAGAACCTCCAGCTCTGCAGGTGTAGGCAGGGCAGCTG; TTACGGAGGCTGCTGAGGAGCTCACCCCCCTGCTCCAGATGAAAAGTGTCTCGTGCTCAA; GTCCCTGTTCACAGGTTGTGAACTTTCCCTTCTTATCCTCTCTCCTTTCTCCCAGAGAGG; CACAGACAGCTCTAGGTGAGTGCTTCCTGTGCATGAATATGTGTGCCATTACCTTGAACA; TGTGGACCTGGGGGTGGGGCTGAGAAGCAATGTCCTTTTAGTCTTTCCCACCCCCACAGC; AGGAACCCAAGGGGAGGGAGGGGACTGGTATTANCAAGCTGAGGGGAGGAGGACTGCTTG; TACACGCTGAATAGGATCTCTACTATCTCAAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_012266
• Insert Size: 2390 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012266.3, NP_036398.3
• RefSeq Size: 2462 bp
• RefSeq ORF: 1047 bp
• Locus ID: 25822
• UniProt ID: O75953
• Cytogenetics: 9p13.3
• Domains: DnaJ, DnaJ_C
• Summary: This gene encodes a member of the DNAJ heat shock protein 40 family of co-chaperone proteins. The encoded protein contains an N-terminal DNAJ domain and a C-terminal substrate binding domain but lacks the cysteine-rich domain found in other DNAJ family members. In mice, a multi-protein complex containing this protein, thioredoxin 1, and histone deacetylase 4, serves as a master negative regulator of cardiac hypertrophy. [provided by RefSeq, Mar 2017]; Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream translation initiation codon, compared to variant 2. The encoded isoform (3) has a shorter N-terminus, compared to isoform 2. Both variants 3 and 5 encode the same isoform (3). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.