Butyrylcholinesterase (BCHE) (NM_000055) Human Untagged Clone

SKU SC115331

BCHE (untagged)-Human butyrylcholinesterase (BCHE)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Butyrylcholinesterase
• Synonyms: BCHED; CHE1; CHE2; E1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115331 sequence for NM_000055 edited (data generated by NextGen Sequencing); ATGCATAGCAAAGTCACAATCATATGCATCAGATTTCTCTTTTGGTTTCTTTTGCTCTGC; ATGCTTATTGGGAAGTCACATACTGAAGATGACATCATAATTGCAACAAAGAATGGAAAA; GTCAGAGGGATGAACTTGACAGTTTTTGGTGGCACGGTAACAGCCTTTCTTGGAATTCCC; TATGCACAGCCACCTCTTGGTAGACTTCGATTCAAAAAGCCACAGTCTCTGACCAAGTGG; TCTGATATTTGGAATGCCACAAAATATGCAAATTCTTGCTGTCAGAACATAGATCAAAGT; TTTCCAGGCTTCCATGGATCAGAGATGTGGAACCCAAACACTGACCTCAGTGAAGACTGT; TTATATCTAAATGTATGGATTCCAGCACCTAAACCAAAAAATGCCACTGTATTGATATGG; ATTTATGGTGGTGGTTTTCAAACTGGAACATCATCTTTACATGTTTATGATGGCAAGTTT; CTGGCTCGGGTTGAAAGAGTTATTGTAGTGTCAATGAACTATAGGGTGGGTGCCCTAGGA; TTCTTAGCTTTGCCAGGAAATCCTGAGGCTCCAGGGAACATGGGTTTATTTGATCAACAG; TTGGCTCTTCAGTGGGTTCAAAAAAATATAGCAGCCTTTGGTGGAAATCCTAAAAGTGTA; ACTCTCTTTGGAGAAAGTGCAGGAGCAGCTTCAGTTAGCCTGCATTTGCTTTCTCCTGGA; AGCCATTCATTGTTCACCAGAGCCATTCTGCAAAGTGGATCCTTTAATGCTCCTTGGGCG; GTAACATCTCTTTATGAAGCTAGGAACAGAACGTTGAACTTAGCTAAATTGACTGGTTGC; TCTAGAGAGAATGAGACTGAAATAATCAAGTGTCTTAGAAATAAAGATCCCCAAGAAATT; CTTCTGAATGAAGCATTTGTTGTCCCCTATGGGACTCCTTTGTCAGTAAACTTTGGTCCG; ACCGTGGATGGTGATTTTCTCACTGACATGCCAGACATATTACTTGAACTTGGACAATTT; AAAAAAACCCAGATTTTGGTGGGTGTTAATAAAGATGAAGGGACAGCTTTTTTAGTCTAT; GGTGCTCCTGGCTTCAGCAAAGATAACAATAGTATCATAACTAGAAAAGAATTTCAGGAA; GGTTTAAAAATATTTTTTCCAGGAGTGAGTGAGTTTGGAAAGGAATCCATCCTTTTTCAT; TACACAGACTGGGTAGATGATCAGAGACCTGAAAACTACCGTGAGGCCTTGGGTGATGTT; GTTGGGGATTATAATTTCATATGCCCTGCCTTGGAGTTCACCAAGAAGTTCTCAGAATGG; GGAAATAATGCCTTTTTCTACTATTTTGAACACCGATCCTCCAAACTTCCGTGGCCAGAA; TGGATGGGAGTGATGCATGGCTATGAAATTGAATTTGTCTTTGGTTTACCTCTGGAAAGA; AGAGATAATTACACAAAAGCCGAGGAAATTTTGAGTAGATCCATAGTGAAACGGTGGGCA; AATTTTGCAAAATATGGGAATCCAAATGAGACTCAGAACAATAGCACAAGCTGGCCTGTC; TTCAAAAGCACTGAACAAAAATATCTAACCTTGAATACAGAGTCAACAAGAATAATGACG; AAACTACGTGCTCAACAATGTCGATTCTGGACATCATTTTTTCCAAAAGTCTTGGAAATG; ACAGGAAATATTGATGAAGCAGAATGGGAGTGGAAAGCAGGATTCCATCGCTGGAACAAT; TACATGATGGACTGGAAAAATCAATTTAACGATTACACTAGCAAGAAAGAAAGTTGTGTG; GGTCTCTAA; ; Clone variation with respect to NM_000055.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000055 unedited; TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATGATTTTCACTCCTTGCAAA; CTTTGCCATCTTTGTTGCAGAGAATCGGAAATCAATATGCATAGCAAAGTCACAATCATA; TGCATCAGATTTCTCTTTTGGTTTCTTTTGCTCTGCATGCTTATTGGGAAGTCACATACT; GAAGATGACATCATAATTGCAACAAAGAATGGAAAAGTCAGAGGGATGAACTTGACAGTT; TTTGGTGGCACGGTAACAGCCTTTCTTGGAATTCCCTATGCACAGCCACCTCTTGGTAGA; CTTCGATTCAAAAAGCCACAGTCTCTGACCAAGTGGTCTGATATTTGGAATGCCACAAAA; TATGCAAATTCTTGCTGTCAGAACATAGATCAAAGTTTTCCAGGCTTCCATGGATCAGAG; ATGTGGAACCCAAACACTGACCTCAGTGAAGACTGTTTATATCTAAATGTATGGATTCCA; GCACCTAAACCAAAAAATGCCACTGTATTGATATGGATTTATGGTGGTGGTTTTCAAACT; GGAACATCATCTTTACATGTTTATGATGGCAAGTTTCTGGCTCGGGTTGAAAGAGTTATT; GTAGTGTCAATGAACTATAGGGTGGGTGCCCTAGGATTCTTAGCTNTGCCAGGAAATCCT; GAGGCTCCAGGGAACATGGGTTTATTTGATCAACAGTTGGCTCTTCAGTGGGTTCAAAAA; AATATAGCAGCCTTTTGTGGAAATCCCTAAAGTGNTACTCTCTNTGGAGAAATGCANGAG; CAGCTTCAGTTAGCCTGCATTTGCTTTCTCCTGNAAGCATTCATTGNTCACCAGANCCAT; CTGCANAGTGGATCCTTTATGCTCCNTTGGCGGTACATCTCTTTATGAAGCTAGACAGAC; GTTGAACTTACTAANTGACTGGTGCTCTAAAGATGAGCTGANTAATCAGGGCTAAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000055 unedited; TTTTAAAANNNNTTACTTGNACGCGGCCGCATTCTANGATCAGTTTTTTTTTTTTTTTTT; TGATTTTCTGTGCTTATTTTAATATCTCATATTGACATTCAATTCTTATTTTAATTAGGA; AACATAATATTGTAAACTATGGTGTACAGTGTTTCAATATTTAATTCATTTAAAAAAGAT; ACCTTTTTACGAGTGGTAATGAAAATACACGTGACTAAAAGCAGAGCACTGATAATTTTG; GGGGGAAAAAACTTAAATTTATTAAGGAAAGAAAGAAATTGAACCAGGCCCATTGTTTTA; ATATGCACATAATTAACTGTAGAACTTTATATTGTGAAATTTAATTAAACATGTTCTAGC; CATTTGGGTTTTTGAATGCTANGTAAAATACTAAGNTTAAAGATGTGAGGAATCAATATT; ATCCTTCTGGCATTTTTGGTTCAGCTACATAATAACTTTTTTTAGTAGGTGTGTAAAAAA; GCTCCTGATATTTTTGCCTTGATCTAAAGGAAAAATATGTCTATAAAAGGGTAANTCTAT; TAATTANAGACCCACACCACTTTCTTTCTGGCTAGGGAAATCCGTAAATGATTTTTCCAG; TCCATCATGTAATTGGTCCNAGGATTGAATCCTGCTTTTCACTCCCATTTGGTTTAATAA; AATTTCCTGGCATTTCCAAAACTTTTGAAAAAATGAAGTCCAAAATCAAATGGTTAGCCC; CGAATTTTCGCATTTTTCTTGGTGACCCTTGTATTAAGGGTAAAAATTTTTTGTAAAGGC; TTTTAAAAACAGCCACTTGGGCCTTTGGTTTGAACCCATTTGGATTCCCATTTTTGCAAA; TTTGCCCCCCTTTCCATTGAGATTACCCAAAATTCCCGCCTTTTGGGAAATAACCTTTTT; TCCAGAGGAAACCAAAAAATTATTTTTTTAGCCTGCCTTTTCCCCCTCTTTTGGCCGAGA; TTTGGGGGACGGGGTTAAAAATAAAAAAAGGTTTTTTCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_000055
• Insert Size: 2430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000055.1, NP_000046.1
• RefSeq Size: 2444 bp
• RefSeq ORF: 1809 bp
• Locus ID: 590
• UniProt ID: P06276
• Cytogenetics: 3q26.1
• Domains: COesterase
• Protein Families: Druggable Genome, Transmembrane
• Summary: This gene encodes a cholinesterase enzyme and member of the type-B carboxylesterase/lipase family of proteins. The encoded enzyme exhibits broad substrate specificity and is involved in the detoxification of poisons including organophosphate nerve agents and pesticides, and the metabolism of drugs including cocaine, heroin and aspirin. Humans homozygous for certain mutations in this gene exhibit prolonged apnea after administration of the muscle relaxant succinylcholine. [provided by RefSeq, Jul 2016]; Transcript Variant: This variant (1) encodes the protein.