AK3L1 (AK4) (NM_013410) Human Untagged Clone

CAT#: SC115256

AK4 (untagged)-Human adenylate kinase 4 (AK4), nuclear gene encoding mitochondrial protein, transcript variant 6

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AK3L1
• Synonyms: AK3; AK3L1; AK3L2; AK 4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115256 sequence for NM_013410 edited (data generated by NextGen Sequencing)
  ATGGCTTCCAAACTCCTGCGCGCGGTCATCCTCGGGCCGCCCGGCTCGGGCAAGGGCACC
  GTGTGCCAGAGGATCGCCCAGAACTTTGGTCTCCAGCATCTCTCCAGCGGCCACTTCTTG
  CGGGAGAACATCAAGGCCAGCACCGAAGTTGGTGAGATGGCAAAGCAGTATATAGAGAAA
  AGTCTTTTGGTTCCAGACCATGTGATCACACGCCTAATGATGTCCGAGTTGGAGAACAGG
  CGTGGCCAGCACTGGCTCCTTGATGGTTTTCCTAGGACATTAGGACAAGCCGAAGCCCTG
  GACAAAATCTGTGAAGTGGATCTAGTGATCAGTTTGAATATTCCATTTGAAACACTTAAA
  GATCGTCTCAGCCGCCGTTGGATTCACCCTCCTAGCGGAAGGGTATATAACCTGGACTTC
  AATCCACCTCATGTACATGGTATTGATGACGTCACTGGTGAACCGTTAGTCCAGCAGGAG
  GATGATAAACCCGAAGCAGTTGCTGCCAGGCTAAGACAGTACAAAGACGTGGCAAAGCCA
  GTCATTGAATTATACAAGAGCCGAGGAGTGCTCCACCAATTTTCCGGAACGGAGACGAAC
  AAAATCTGGCCCTACGTTTACACACTTTTCTCAAACAAGATCACACCTATTCAGTCCAAA
  GAAGCATATTGA
  
  Clone variation with respect to NM_013410.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013410 unedited
  NTTGTCGGATTTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACCAGGGTGCGTG
  GGGCGGCGGGGGCTGGCTTTGAGTCACCCCCGCTTGTGCCAGCTTCCCCGGCTCGGCGGA
  GACTGGCTTGGGGATGCCTGGCCGCCCGGCCCCCTTCTCGGACGCGGCGTGGGGAGCGAG
  CTGGAGTTAAGGATGAAGGGGCGGCCGGCGAGTCCCAGTGAGAGCGGAGGGTGCCAGAGG
  TAGGGGGCCGAGAAACAAAGTTCCCGGGGCTTCCTCCGGGGCCGCGGTCGGGGCTGCGCG
  TTTGACCGCCCCCCTCCTCGCGAAGGCAATGGCTTCCAAACTCCTGCGCGCGGTCATCCT
  CGGGCCGCCCGGCTCGGGCAAGGGCACCGTGTGCCAGAGGATCGCCCAGAACTTTGGTCT
  CCAGCATCTCTCCAGCGGCCACTTCTTGCGGGAGAACATCAAGGCCAGCACCGAAGTTGG
  TGAGATGGCAAAGCAGTATATAGAGAAAAGTCTTTTGGTTCCAGACCATGTGATCACACG
  CCTAATGATGTCCGAGTTGGAGAACAGGCGTGGCCAGCACTGGCTCCTTGATGGTTTTCC
  TAGGACATTAGGACAAGCCGAAGCCCTGGACAAAATCTGTGAAGTGGATCTAGTGATCAG
  TTTGAATATTCCATTTGAAACCTTAAAGATCGTCTAGCCGCCGTTGGATTCACCCTCCTA
  GCGGAAGGGTATATAACCTGGACTTCATCCACCTCATGTACATGGTATGGATGACGTCAC
  TGGTGAACCGTTAGTCCGCAGGAGGATGATAACCCCAAACAGTTGCTGCCGGCTAAGAAG
  TCCAAAGACGTGGCAAGCCAGTCATTGATTATACAGAGCCCCGAGAGTGCTCCACCATTT
  TCCGGAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013410 unedited
  NNTTTTTACTCTGGACCCGCGGCCGCATNCTAGNGTCGAGTTTTTTTTTTTTTTTTTTTT
  TTTTTCCCAATAAAAAAAAGGACAGGGTCAATCCATAAAGTCAGACCAGCAGTTTAATAA
  AATCCTCTTCATAGCTCAGTTCTCAGGGCATACAGAGATTCAATATAGCCCCATCGCTCT
  CAGTTATTAAAATCTGAGAGGGATAAAAGCAATAACTATTGTTTAAAAGCCTAAGAGTGA
  AAACAGATGTCCAAAAAAAAAAAGAAAAAAAAAAGGCCAAATTCTTTCATCCCAGGATGT
  CTGAAAAGGAGAGACTCTATGGAGCGTGGATGTAAAAAAGGAGTTATTCCTACCGGAAAA
  AAATGAACTGGGGACACAGTCACTCAAGACCAGAGAAACAACCACTATGAGTAAGTAGCT
  TCAATTTCACTGTTACCAGGAGGGCACACACCAGAAAGCCTGGGACAAAGCCTGGGCTAA
  AGCCCTCAGGATTCATTCTCAGGCTTCCTGGTGCTGTGGTTCAACAGCCTTTGTGGGGAT
  GGGGAGAACCAGAGACTATCACCATCACATGTCAAAAATATTACCACAGCAACAGCTGTC
  AAAAACCAGCTGGAGCTGAAGTTACACACCATTTGAAATCCACACTGTACTTGCTTTCTT
  GTGATGGGCTTGAGACATGTGCAAAGACACCTTAAACATGTGTAGGTGACCAGCCTTTTG
  AAAGGCAGAGGAGGGCCTCTTCATGAACTCTTTCTACTCATTTGTTTTCCTATCAGTTCA
  CCATTTCAACTAGAAAAGATGCTGNCGGCTACCTCAGCTAGCTTCTAATTTGGACACAGC
  ACCAATACTACACACACTATTGAATGAATGACCACATCTTCCTGGGTNCTTCCATTGGNG
  CAGGGTCAATATNGCTNCTTTGGACTGAATANGNTNGTGATNCTTGTNTGANAAANAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_013410
• Insert Size: 2050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013410.2, NP_037542.1
• RefSeq Size: 2199 bp
• RefSeq ORF: 672 bp
• Locus ID: 205
• UniProt ID: P27144
• Cytogenetics: 1p31.3
• Domains: ADK, ADK_lid
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism
• Gene Summary: This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 all encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.