USP25 (NM_013396) Human Untagged Clone

SKU SC115248

USP25 (untagged)-Human ubiquitin specific peptidase 25 (USP25)

Product Data

• Type: Human Untagged Clone
• Target Symbol: USP25
• Synonyms: USP21
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115248 sequence for NM_013396 edited (data generated by NextGen Sequencing); ATGACCGTGGAGCAGAACGTGCTGCAGCAGAGCGCGGCGCAGAAGCACCAGCAGACGTTT; TTGAATCAACTGAGAGAAATTACGGGGATTAATGACACCCAGATACTACAGCAAGCCTTG; AAGGATAGTAATGGAAACTTGGAATTAGCAGTGGCTTTCCTTACTGCGAAGAATGCTAAG; ACCCCTCAGCAGGAGGAGACAACTTACTACCAAACAGCACTTCCTGGCAATGATAGATAC; ATCAGTGTGGGAAGCCAAGCAGATACAAATGTGATTGATCTCACTGGAGATGATAAAGAT; GATCTTCAGAGAGCAATTGCCTTGAGTTTGGCCGAATCAAACAGGGCATTCAGGGAGACT; GGAATAACTGATGAGGAACAAGCCATTAGCAGAGTTCTTGAAGCCAGCATAGCAGAGAAT; AAAGCATGTTTGAAGAGGACACCTACAGAAGTTTGGAGGGATTCTCGAAACCCTTATGAT; AGAAAAAGACAGGACAAAGCTCCCGTTGGGCTAAAGAATGTTGGCAATACTTGTTGGTTT; AGTGCTGTTATTCAGTCATTATTTAATCTTTTGGAATTTAGAAGATTAGTTCTGAATTAC; AAGCCTCCATCAAATGCTCAAGATTTACCCCGAAACCAAAAGGAACATCGGAATTTGCCT; TTTATGCGTGAGCTGAGGTATCTATTTGCACTTCTTGTTGGTACCAAAAGGAAGTATGTT; GATCCATCAAGAGCAGTTGAAATTCTTAAGGATGCTTTCAAATCAAATGACTCACAGCAG; CAAGATGTGAGTGAGTTTACACACAAATTATTAGATTGGTTAGAAGATGCCTTCCAAATG; AAAGCTGAAGAGGAGACGGATGAAGAGAAGCCAAAGAACCCCATGGTAGAGTTGTTCTAT; GGCAGATTCCTGGCTGTGGGAGTACTTGAAGGTAAAAAATTTGAAAACACTGAAATGTTT; GGTCAGTACCCACTTCAGGTCAATGGGTTCAAAGATCTGCATGAGTGCCTAGAAGCTGCA; ATGATTGAAGGAGAAATTGAGTCTTTACATTCAGAGAATTCAGGAAAATCAGGCCAAGAG; CATTGGTTTACTGAATTACCACCTGTGTTAACATTTGAATTGTCAAGATTTGAATTTAAT; CAGGCATTGGGAAGACCAGAAAAAATTCACAACAAATTAGAATTTCCCCAAGTTTTATAT; TTGGACAGATACATGCACAGAAACAGAGAAATAACAAGAATTAAGAGGGAAGAGATCAAG; AGACTGAAAGATTACCTCACGGTATTACAACAAAGGCTAGAAAGATATTTAAGCTATGGT; TCCGGTCCCAAACGATTCCCCTTGGTAGATGTTCTTCAGTATGCATTGGAATTTGCCTCA; AGTAAACCTGTTTGCACTTCTCCTGTTGACGATATTGACGCTAGTTCCCCACCTAGTGGT; TCCATACCATCACAGACATTACCAAGCACAACAGAACAACAGGGAGCCCTATCTTCAGAA; CTGCCAAGCACATCACCTTCATCAGTTGCTGCCATTTCATCGAGATCAGTAATACACAAA; CCATTTACTCAGTCCCGGATACCTCCAGATTTGCCCATGCATCCGGCACCAAGGCACATA; ACGGAGGAAGAACTTTCTGTGCTGGAAAGTTGTTTACATCGCTGGAGGACAGAAATAGAA; AATGACACCAGAGATTTGCAGGAAAGCATATCCAGAATCCATCGAACAATTGAATTAATG; TACTCTGACAAATCTATGATACAAGTTCCTTATCGATTACATGCCGTTTTAGTTCACGAA; GGCCAAGCTAATGCTGGGCACTACTGGGCATATATTTTTGATCATCGTGAAAGCAGATGG; ATGAAGTACAATGATATTGCTGTGACAAAATCATCATGGGAAGAGCTAGTGAGGGACTCT; TTTGGTGGTTATAGAAATGCCAGTGCATACTGTTTAATGTACATAAATGATAAGGCACAG; TTCCTAATACAAGAGGAGTTTAATAAAGAAACTGGGCAGCCCCTTGTTGGTATAGAAACA; TTACCACCGGATTTGAGAGATTTTGTTGAGGAAGACAACCAACGATTTGAAAAAGAACTA; GAAGAATGGGATGCACAACTTGCCCAGAAAGCTTTGCAGGAAAAGCTTTTAGCGTCTCAG; AAATTGAGAGAGTCAGAGACTTCTGTGACAACAGCACAAGCAGCAGGAGACCCAGAATAT; CTAGAGCAGCCATCAAGAAGTGATTTCTCAAAGCACTTGAAAGAAGAAACTATTCAAATA; ATTACCAAGGCATCACATGAGCATGAAGATAAAAGTCCTGAAACAGTTTTGCAGTCGGCA; ATTAAGTTGGAATATGCAAGGTTGGTTAAGTTGGCCCAAGAAGACACCCCACCAGAAACC; GATTATCGTTTACATCATGTAGTGGTCTACTTTATCCAGAACCAGGCACCAAAGAAAATT; ATTGAGAAAACATTACTAGAACAATTTGGAGATAGAAATTTGAGTTTTGATGAAAGGTGT; CACAACATAATGAAAGTTGCTCAAGCCAAACTGGAAATGATAAAACCTGAAGAAGTAAAC; TTGGAGGAATATGAGGAGTGGCATCAGGATTATAGGAAATTCAGGGAAACAACTATGTAT; CTCATAATTGGGCTAGAAAATTTTCAAAGAGAAAGTTATATAGATTCCTTGCTGTTCCTC; ATCTGTGCTTATCAGAATAACAAAGAACTCTTGTCTAAAGGCTTATACAGAGGACATGAT; GAAGAATTGATATCACATTATAGAAGAGAATGTTTGCTAAAATTAAATGAGCAAGCCGCA; GAACTCTTCGAATCTGGAGAGGATCGAGAAGTAAACAATGGTTTGATTATCATGAATGAG; TTTATTGTCCCATTTTTGCCATTATTACTGGTGGATGAAATGGAAGAAAAGGATATACTA; GCTGTAGAAGATATGAGAAATCGATGGTGTTCCTACCTTGGTCAAGAAATGGAACCACAC; CTCCAAGAAAAGCTGACAGATTTTTTGCCAAAACTGCTTGATTGTTCTATGGAGATTAAA; AGTTTCCATGAGCCACCGAAGTTACCTTCATATTCCACGCATGAACTCTGTGAGCGATTT; GCCCGAATCATGTTGTCCCTCAGTCGAACTCCTGCTGATGGAAGATAA; ; Clone variation with respect to NM_013396.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013396 unedited; AGGTCAGAAATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGAGCCCG; CGGACCGGCAGCAAAGGACGTGCGAACGCGTGACGCCGCCCGACTGGCTCGCGCTCTCCC; GTGCCCCGGCGTCCTCCGCCCGCTCATGGCCCGGGCCGCCGCGGACGAGCGGCGCTGAGG; CGGGCCGCGTGGAGACGTGAGGCGGCCGCCGTGGCCCTCACAGTCGGCGTTTCGCCGCCT; GCCCGCGGTGCCCGCGCACGCCGGCCGCCATCGCCTTCGCGCCTGGCTGGCGGGGGCGCT; GTCCTCCCAGGCCGTCCGCGCCGCTCCCTGGAGCTCGGCGGAGCGCGGCAGCCAGGGCCG; GCGGAGGCGCGAGGAGCCGGGCGCCACCGCCGCCGCCGCCGCCGCCGCCGCGGGGGCCAT; GACCGTGGAGCAGAACGTGCTGCAGCAGAGCGCGGCGCAGAAGCACCAGCAGACGTTTTT; GAATCAACTGAGAGAAATTACGGGGATTAATGACACCCAGATACTACAGCAAGCCTTGAA; GGATAGTAATGGAAACTTGGAATTAGCAGTGGCTTTCCTTACTGCGAAGAATGCTAAGAC; CCCTCAGCAGGAGGAGACAACTTACTACCAAACAGCACTTCCTGGCAATGATAGATACAT; CAGTGTGGGAAGCCAAGCAGATACAAATGTGATTGATCTCACTGGAGATGATAAAGATGA; TCCTCAGAGAGCAATTGCCTTGAGTTTGGCCCGATCAAACAGGGCATTCAGGCAGACTGG; AATAACTGATGACGAACAAGCCCTTACCAGAGTTCTTTGAACCAGCATACCAGAGAATAA; GCCTGTTTTGAAGAAGACCCTACAGAAGTTGTGGAGGGATTCTCCAAACCCTTATGATAG; A;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013396 unedited; ATGGACCGCGGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTAGCCATAAATAA; AACTTTATTCTTTATTTCATTTACAAGCTACCAAATATTATGTATCGTACACAGTGCTGA; ACACTTAAATGGCTGTAGTCATGGAAGGATCCAGACTGAATGGAAAGCTGTTGAGAAAGA; AAAGATAAAAGCAAAGTAATACTGCAACAGGAAGGTGGCAAAAGCATAGTTTTGCCATAA; TAAAATCAATTAGATTTGTGATTATACATCAGTTCCGGTTAAAATGTCTGAGCGCCATGC; GATTTTCAGCTTTATTGTCTGCAGTCTGACTAAAGTCTGTATAGTCATTTTGTCTTTTGC; AGTTATTAAAATAAAAAAAAGTTAAAAACTATAGCAGCAACAAGCAAACCCTGTGACAGG; AAGGCAAGGGTTAAGAACTAAAAAGAGTTTATACAGTGTGTTCAGGGAAAGTGTGCAGTT; TATCTTCCATCAGCAGGAGTTCGACTGAGGGACAACATGATTCGGGCAAATCGCTCACAG; AGTTCATGCGTGGAATATGAAGGTAACTTCGGTGGCTCATGGAAACTTTTATCTCCTAGA; ACAATCAAGCAGTTTTGGCAAAAAATCTGTCCACTTTTTTTGGAAGTGTGGTTCCCTTTC; TTGACCAAGGTAGGAACCCCATCGGATTCTCATATTTTTTACAGCTAAGATATCCTCTTT; TTCCTTTTCATCCACCAGTATTAATGGCAAAAATGGGACAATTAACTTTATTCTTGATAA; TTAACCCTTTGTTTCCTTCTTGAACCCCCCCAAATCCAAAATTTTGGCGTTTGCCCTTTA; ATTTACAAAAATTTCTTTTTTATGCGACAAAATTCTTATCATTCCCCTGTTTACACCTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_013396
• Insert Size: 4500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_013396.3, NP_037528.3
• RefSeq Size: 4968 bp
• RefSeq ORF: 3168 bp
• Locus ID: 29761
• UniProt ID: Q9UHP3
• Cytogenetics: 21q21.1
• Domains: UCH, UIM
• Protein Families: Protease
• Summary: Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]).[supplied by OMIM, Mar 2008]; Transcript Variant: This variant (3) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1, resulting in an isoform (USP25a) that is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.