ITPK1 (NM_014216) Human Untagged Clone

SKU SC115136

ITPK1 (untagged)-Human inositol-tetrakisphosphate 1-kinase (ITPK1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: ITPK1
• Synonyms: ITRPK1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115136 sequence for NM_014216 edited (data generated by NextGen Sequencing); ATGCAGACCTTTCTGAAAGGGAAGAGAGTTGGCTACTGGCTGAGCGAGAAGAAAATCAAG; AAGCTGAATTTCCAGGCCTTCGCCGAGCTGTGCAGGAAGCGAGGGATGGAGGTTGTGCAG; CTGAACCTTAGCCGGCCGATCGAGGAGCAGGGCCCCCTGGACGTCATCATCCACAAGCTG; ACTGACGTCATCCTTGAAGCCGACCAGAATGATAGCCAGTCCCTGGAGCTGGTGCACAGG; TTCCAGGAGTACATCGATGCCCACCCTGAGACCATCGTCCTGGACCCGCTCCCTGCCATC; AGAACCCTGCTTGACCGCTCCAAGTCCTATGAGCTCATCCGGAAGATTGAGGCCTACATG; GAAGACGACAGGATCTGCTCGCCACCCTTCATGGAGCTCACGAGCCTGTGCGGGGATGAC; ACCATGCGGCTGCTGGAGAAGAACGGCTTGACTTTCCCATTCATTTGCAAAACCAGAGTG; GCTCATGGCACCAACTCTCACGAGATGGCTATCGTGTTCAACCAGGAGGGCCTGAACGCC; ATCCAGCCACCCTGCGTGGTCCAGAATTTCATCAACCACAACGCCGTCCTGTACAAGGTG; TTCGTGGTTGGCGAGTCCTACACCGTGGTCCAGAGGCCCTCACTCAAGAACTTCTCCGCA; GGCACATCAGACCGTGAGTCCATCTTCTTCAACAGCCACAACGTGTCAAAGCCGGAGTCG; TCATCGGTCCTGACGGAGCTGGACAAGATCGAGGGCGTGTTCGAGCGGCCGAGCGACGAG; GTCATCCGGGAGCTCTCCCGGGCCCTGCGGCAGGCACTGGGCGTGTCACTCTTTGGCATC; GACATCATCATCAACAACCAGACAGGGCAGCACGCCGTCATTGACATCAATGCCTTCCCA; GGCTACGAGGGCGTGAGCGAGTTCTTCACAGACCTCCTGAACCACATCGCCACTGTCCTG; CAGGGCCAGAGCACAGCCATGGCAGCCACAGGGGACGTGGCCCTGCTGAGGCACAGCAAG; CTTCTGGCCGAGCCGGCGGGCGGCCTGGTGGGCGAGCGGACATGCAGCGCCAGCCCCGGC; TGCTGCGGCAGCATGATGGGCCAGGACGCGCCCTGGAAGGCTGAGGCCGACGCGGGCGGC; ACCGCCAAGCTGCCGCACCAGAGACTCGGCTGCAACGCCGGCGTGTCGCCCAGCTTCCAG; CAGCATTGTGTGGCCTCCCTGGCCACCAAGGCCTCCTCCCAGTAG; ; Clone variation with respect to NM_014216.4; 834 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014216 unedited; NGGTCAAAATTTGTATACGACTCCTATAGGCGGCNCGCGATTCGGCACGAGGCCGCCGGG; AGGAGGTGCCCACTCGCTCGCGGCGCGCGCCGGCCGCCAGACTCGGCCTGTGGGCGATTT; CCTCCGGACCCAGGCTCCCCGCCCGAGGAGGAAGATGCAGACCTTTCTGAAAGGGAAGAG; AGTTGGCTACTGGCTGAGCGAGAAGAAAATCAAGAAGCTGAATTTCCAGGCCTTCGCCGA; GCTGTGCAGGAAGCGAGGGATGGAGGTTGTGCAGCTGAACCTTAGCCGGCCGATCGAGGA; GCAGGGCCCCCTGGACGTCATCATCCACAAGCTGACTGACGTCATCCTTGAAGCCGACCA; GAATGATAGCCAGTCCCTGGAGCTGGTGCACAGGTTCCAGGAGTACATCGATGCCCACCC; TGAGACCATCGTCCTGGACCCGCTCCCTGCCATCAGAACCCTGCTTGACCGCTCCAAGTC; CTATGAGCTCATCCGGAAGATTGAGGCCTACATGGAAGACGACAGGATCTGCTCGCCACC; CTTCATGGAGCTCACGAGCCTGTGCGGGGATGACACCATGCGGCTGCTGGAGAAGAACGG; CTTGACTTTCCCATTCATTTGCAAAACCAGAGTGGCTCATGGCACCAACTCTCACGAGAT; GGCTATCGTGTTCAACCAGGAGGGCCTGAACGCCATCCAGCCACCCTGCGTGGTCCAGAA; TTTCATCAACCACAACGCCGTCCTGGTACAAGGTGTNCGTGGTTGGCGAGTCCTACACCG; TGGTCCAGAAGCCCTCACTCAAAANCTTCTNCGCAGCACATCAGACCGTGAGTCCATCTT; CTTCACAGCCACCACGTGTCAAGCCGGNAGTCGTCATCGGTCCTGACGGAGCTGGACCAG; ATCNAGGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014216 unedited; TTGGCCCGCGGCTCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCGGTTTGATTT; CAGTAGTTTATTTTGGAGACAAAGCAGTGCAAGAGGCCAGCCACGCTTTCCTGTTCCCCA; GGGCTCAGGGTCAGAACTAGGAAAGGTGACGTACAGACATTAATCGGGGTTCAAAACTCA; AGTCGTGTAAACGTGGTTAGTTGTTGGGTCCTCAGTTTCCAAAAAAGCCGGGCAGTTCTG; ATGGCCTCTGCCCCTCGCCCAAGCCCTGTGCCTCCCTCCTCAAAGTGGGCATTCTGCAGC; CCAGCTGAGCCAGGCCGAAGGACCTCCATGCACTGGCTCGGGGGCCTCTCTCGGGACACT; CAGCACTTTCTCTAGCGTCCTCCATCTCACTGGGCAGAGGACAGCCCGGAAGCCTTTTTC; ACCTTTTTCAAAGTAAACTGCTATCTTAAGACACAAAAACATACTTGTGGGGGCTGATGC; CTTTTACCCAGCACCCCACATCTTCCAGGACTGCAGAGGCTTTTCCCCATCCCTTTTCTC; TGTGGAGGGGGCTGCCTGGATCAGGGGTCACACCGCAGACCCCGTGCTTTGCTGCCCAGC; GATGGGGCGAGTCTGAGGTGCGCATACACGATGGGGGGCGGGTGGCTCCCTGGCACCCGC; TGGCTCATTGCCTTGGGCTCGAAATCAGAGCTGGCACCATCCTGTTGGGTGACGCGGGAC; CCATCAGGTCGTGACAAGGATTGCCCCCACTGGGGATCACGGTCCCGATCTCCATGCTGT; TATGCCGAGCTTCTCGCGCTCATTTCCCTTACGTGCCCNTATGTCAAGCAGCCTGCTTCC; ACGGGTTTGGCTACATACCCCCCCTGTGCGCCTTTTGTTACTAAATGGCATTTAAGACCA; AGGGGGGCCCGCCAAAGCCTCGCCCGCTTGTCATATCCCATACCATTTTTCTCCGCCCCT; CCGGCTCGTTTTCATACGCCCTTCTCCCGCTTCCGAGTCCAACG;
• Restriction Sites: ECoRI-NOT
• ACCN: NM_014216
• Insert Size: 3090 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014216.3, NP_055031.2
• RefSeq Size: 3197 bp
• RefSeq ORF: 1245 bp
• Locus ID: 3705
• UniProt ID: Q13572
• Cytogenetics: 14q32.12
• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Summary: This gene encodes an enzyme that belongs to the inositol 1,3,4-trisphosphate 5/6-kinase family. This enzyme regulates the synthesis of inositol tetraphosphate, and downstream products, inositol pentakisphosphate and inositol hexakisphosphate. Inositol metabolism plays a role in the development of the neural tube. Disruptions in this gene are thought to be associated with neural tube defects. A pseudogene of this gene has been identified on chromosome X. [provided by RefSeq, Jul 2016]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Both variants 1 and 2 encode the same isoform.