Calpain 7 (CAPN7) (NM_014296) Human Untagged Clone

SKU SC115098

CAPN7 (untagged)-Human calpain 7 (CAPN7)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Calpain 7
• Synonyms: CALPAIN7; PALBH
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115098 sequence for NM_014296 edited (data generated by NextGen Sequencing); ATGGACGCCACAGCACTGGAGCGGGACGCTGTGCAGTTCGCCCGTCTGGCGGTTCAGCGC; GACCACGAAGGCCGCTACTCCGAGGCGGTGTTTTATTACAAGGAAGCTGCACAAGCCTTA; ATTTATGCTGAGATGGCAGGATCAAGCCTAGAAAATATTCAAGAAAAAATAACTGAGTAT; CTGGAAAGAGTTCAAGCTCTACATTCAGCAGTTCAGTCAAAGAGTGCTGATCCTTTGAAG; TCAAAACATCAGTTGGACTTAGAGCGTGCTCATTTCCTTGTTACACAAGCTTTTGATGAA; GATGAAAAAGAGAATGTTGAAGATGCTATAGAATTGTACACAGAAGCTGTGGATCTCTGT; CTGAAAACATCTTATGAAACTGCTGATAAAGTCCTGCAAAATAAACTGAAACAGTTGGCT; CGACAGGCACTAGACAGAGCAGAAGCGCTGAGTGAGCCTTTGACCAAGCCAGTTGGCAAA; ATCAGTTCAACAAGTGTTAAGCCAAAGCCACCTCCAGTGAGAGCACATTTTCCACTGGGC; GCTAATCCCTTCCTTGAAAGACCTCAGTCATTTATAAGTCCTCAGTCATGTGATGCACAA; GGACAGAGATACACAGCAGAAGAAATAGAAGTACTCAGGACAACATCAAAAATAAATGGT; ATAGAATATGTTCCTTTCATGAATGTTGACCTGAGAGAACGTTTTGCCTATCCAATGCCT; TTCTGTGATAGATGGGGCAAGCTACCATTATCACCTAAACAAAAAACTACATTTTCCAAG; TGGGTACGACCAGAAGACCTCACCAACAATCCTACAATGATATATACTGTGTCCAGTTTT; AGCATAAAGCAGACAATAGTATCGGATTGCTCCTTTGTGGCATCACTGGCCATCAGTGCA; GCTTATGAAAGACGTTTTAATAAGAAGTTAATTACCGGCATAATTTACCCTCAAAACAAG; GATGGTGAACCAGAATACAATCCATGTGGGAAGTATATGGTAAAACTTCACCTCAATGGT; GTCCCAAGAAAGGTGATAATTGATGACCAGTTACCTGTTGATCACAAGGGAGAATTGCTC; TGTTCTTATTCCAACAACAAAAGTGAATTATGGGTTTCTCTCATAGAAAAAGCATACATG; AAAGTCATGGGAGGATATGATTTTCCAGGATCCAACTCCAATATTGATCTTCATGCACTG; ACTGGCTGGATACCAGAAAGAATTGCTATGCATTCAGATAGCCAAACTTTCAGTAAGGAT; AATTCTTTCAGAATGCTTTATCAAAGATTTCACAAAGGAGATGTCCTCATCACTGCGTCA; ACTGGAATGATGACAGAAGCTGAAGGAGAGAAGTGGGGTCTGGTTCCCACACACGCATAT; GCTGTTTTGGATATTAGAGAGTTCAAGGGGCTGCGATTTATCCAGTTGAAAAATCCTTGG; AGTCATTTACGTTGGAAAGGAAGATACAGTGAAAATGATGTAAAAAACTGGACTCCAGAG; TTGCAAAAGTATTTAAACTTTGATCCCCGAACAGCTCAGAAAATAGACAACGGAATATTT; TGGATTTCCTGGGATGATCTCTGCCAGTATTATGATGTGATTTATTTGAGTTGGAATCCA; GGTCTTTTTAAAGAATCAACATGTATTCACAGTACTTGGGATGCTAAGCAAGGACCTGTG; AAAGATGCCTATAGCCTGGCCAACAACCCCCAGTACAAACTGGAGGTGCAGTGTCCACAG; GGGGGTGCTGCAGTTTGGGTTTTGCTTAGTAGACACATAACAGACAAGGATGATTTTGCG; AATAATCGAGAATTTATCACAATGGTTGTATACAAGACTGATGGGAAAAAAGTTTATTAC; CCAGCTGACCCACCTCCATACATTGATGGAATTCGAATTAACAGCCCTCATTATTTGACT; AAGATAAAGCTGACCACACCTGGCACCCATACCTTTACATTAGTGGTTTCTCAATATGAA; AAACAGAACACAATCCATTACACGGTTCGGGTATATTCAGCATGCAGCTTTACTTTTTCA; AAGATTCCTTCACCATACACCTTATCAAAACGGATTAATGGAAAGTGGAGTGGTCAGAGT; GCTGGAGGATGTGGAAATTTCCAAGAGACTCACAAAAATAACCCCATCTACCAATTCCAT; ATAGAAAAGACTGGGCCGTTACTGATTGAGCTACGAGGACCAAGGCAATATAGCGTTGGA; TTTGAGGTTGTAACAGTTTCTACTCTAGGAGATCCTGGTCCCCATGGCTTTCTGAGGAAA; TCTAGTGGTGACTATAGGTGTGGGTTTTGCTACCTGGAATTAGAAAATATACCTTCTGGG; ATCTTCAATATCATTCCTAGTACCTTTTTGCCTAAACAAGAAGGACCTTTTTTCTTGGAC; TTTAATAGTATTATCCCCATCAAGATCACACAATTTCAGTGA; ; Clone variation with respect to NM_014296.2; 2434 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014296 unedited; GATTTTGTATACGACTCACTATAGGCGGCCGCGACATTCGCACGAGGGCCGCGAAGTGGG; GCGGCCGGGTGGGCTACGAGCCGGGTCTGGGCTGAGGGGCGCGGCTTCGCGGTGGACCCC; AGCCCGGCAACGGGAAGGCGAGCTCTCCTCCACCGTCCAAAGTAAACTTTGCCGCTCCTT; CCGCGGCGCTCCCGAGTCCTCGCCGCCGCCGGGCCGCCGCAGTCCGCGAAGAGCCGTCCT; GCGTCAGGGCCTCCTTCCCTGCCCCGGAGCGGGGCCACTGCGCCATGGACGCCACAGCAC; TGGAGCGGGACGCTGTGCAGTTCGCCCGTCTGGCGGTTCAGCGCGACCACGAAGGCCGCT; ACTCCGAGGCGGTGTTTTATTACAAGGAAGCTGCACAAGCCTTAATTTATGCTGAGATGG; CAGGATCAAGCCTAGAAAATATTCAAGAAAAAATAACTGAGTATCTGGAAAGAGTTCAAG; CTCTACATTCAGCAGTTCAGTCAAAGAGTGCTGATCCTTTGAAGTCAAAACATCAGTTGG; ACTTAGAGCGTGCTCATTTCCTTGTTACACAAGCTTTTGATGAAGATGAAAAAGAGAATG; TTGAAGATGCTATAGAATTGTACACAGAAGCTGTGGATCTCTGTCTGAAAACATCTTATG; AAACTGCTGATAAAGTCCTGCAAAATAAACTGAAACAGTTGGCTCGACAGGCACTAGACA; GAGCAGAAGCGCTGAGTGAGCCTTTGACCAAGCCAGTTGGCAAAATCAGTTCAACAAGTG; TTAAGCCAAAGCCACCTCCAGTGAGAGCACATTTTCCACTGGGCGCTAATNCCTTCCTTG; AAGACCTCAGTCATTATAAGTCCTCAGTCATGTGATGCACAGGACAGAGATCACAGCNGA; AGAAATAGAAGTCTCAGGACACATCAAAAATAATGGTNTANAATATGTCCTTTATGAATG; TGACCTGAAGACGTNTTGCTTCCATGCCTTCTGGGAAGAGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014296 unedited; NAAAATACTTGTACGCGGCCGCATTTTANGATCGATTTTTTTTTTTTTTTTTTATCCGTA; AGTATAAACATTTTATTTCAATTTAAGGTAGTAGCAATACAAAATAAGTTTTGATAATTA; TAAAGTAGAGACAATGAAAAATCCCACCCTATTTTTAGAATTTTAAAATATTGAGCATCA; TTCTGTCCTGATGGGTTTTTATGTAAAGTGGGAATTTACATGACACCAGTATCTTGGCAA; TTCACTTTTTTTGAATGCTACATATCCCAATCACCATATATTAAATTCTCTATTTTCTTC; AAAGTGCTATTGCCACAAGAGACTGAACGATACAGCAATTTAAAAAAAGACCACACAAAC; TAAATTGTAATGTCTTCAATATCTCAATTACTGTTTTTTAAAAATAAAAACTGCAGTAGT; GAATTTCTTTAAGTTCTTGCTACCTGATTGAATGGGAGAATACCTCAACAATTTTCATTC; TTTAGCATGAGCATCTTCATGCTAAGGTAGTCCATACTTAAAAGCTTTTATTCTTTCTTA; ACACCTGGGCCACCAATATTTTAAAAATACATAAATAAGGCCAAAATTAAAATCCAGTTG; TGGGGGCAAAGTTTCATTTGGGTAACCACGTTGCCCAAAAATGTTTAGGGAAAATACCAC; CCGGCTTTTAATTTCCCACTGCCCCTGCGTGAAAAGGGGAACACCGGGGAAAATGGTCCC; GGGTTAGTGAGACGAAGGCGCCCTTATTCCGGTGCGAGGGAGGGATGGTCGGCTTTTCTT; TGTGCTGACGGTGGGGTGCGGGCCTCTTTATAACCGCCGACAAACGAAGACCTTTTTGGA; ATGTATGATGACGCCGTCATGATGTTTATATTCTCTCGGGCTATTGTTCTTACCAGATTA; GGGGCGCGCAGTGCGCGGACGCGAGTAGCCTTTATGGTCCCGCAGGCGCACTGGGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_014296
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014296.2, NP_055111.1
• RefSeq Size: 4385 bp
• RefSeq ORF: 2442 bp
• Locus ID: 23473
• UniProt ID: Q9Y6W3
• Cytogenetics: 3p25.1
• Domains: Calpain_III, MIT
• Protein Families: Druggable Genome, Protease
• Summary: Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The function of the protein encoded by this gene is not known. An orthologue has been found in mouse but it seems to diverge from other family members. The mouse orthologue is thought to be calcium independent with protease activity. [provided by RefSeq, Jul 2008]