GADD34 (PPP1R15A) (NM_014330) Human Untagged Clone

CAT#: SC115045

PPP1R15A (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 15A (PPP1R15A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GADD34
• Synonyms: GADD34
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_014330 edited
  GAATTCGGCACGAGGCCCAGTTGTTGATCTTATGCAAGACGCTGCACGACCCCGCGCCCG
  CTTGTCGCCACGGCACTTGAGGCAGCCGGAGATACTCTGAGTTACTCGGAGCCCGACGCC
  TGAGGGTGAGATGAACGCGCTGGCCTCCCTAACCGTCCGGACCTGTGATCGCTTCTGGCA
  GACCGAACCGGCGCTCCTGCCCCCGGGGTGACGCGCAGCCCCCAGCCGCCCAGACACATG
  GCCCCAGGCCAAGCACCCCATCAGGCTACCCCGTGGAGGGATGCCCACCCTTTCTTCCTC
  CTGTCCCCAGTGATGGGCCTCCTCAGCCGCGCCTGGAGCCGCCTGAGGGGCCTGGGACCT
  CTAGAGCCCTGGCTGGTGGAAGCAGTAAAAGGAGCAGCTCTGGTAGAAGCTGGCCTGGAG
  GGAGAAGCTAGGACTCCTCTGGCAATCCCCCATACCCCTTGGGGCAGACGCCCTGAAGAG
  GAGGCTGAAGACAGTGGAGGCCCTGGAGAGGACAGAGAAACACTGGGGCTGAAAACCAGC
  AGTTCCCTTCCTGAAGCCTGGGGACTTTTGGATGATGATGATGGCATGTATGGTGAGCGA
  GAGGCAACCAGTGTCCCTAGAGGGCAGGGAAGTCAATTTGCAGATGGCCAGCGTGCTCCC
  CTGTCTCCCAGCCTTCTGATAAGGACACTGCAAGGTTCTGATAAGAACCCAGGGGAGGAG
  AAAGCCGAGGAAGAGGGAGTTGCTGAAGAGGAGGGAGTTAACAAGTTCTCTTATCCACCA
  TCACACCGGGAGTGTTGTCCAGCCGTGGAGGAGGAGGACGATGAAGAAGCTGTAAAGAAA
  GAAGCTCACAGAACCTCTACTTCTGCCTTGTCTCCAGGATCCAAGCCCAGCACTTGGGTG
  TCTTGCCCAGGGGAGGAAGAGAATCAAGCCACGGAGGATAAAAGAACAGAAAGAAGTAAA
  GGAGCCAGGAAGACCTCCGTGTCCCCCCGATCTTCAGGCTCCGACCCCAGGTCCTGGGAG
  TATCGTTCAGGAGAGGCGTCCGAGGAGAAGGAGGAAAAGGCACACGAAGAAACTGGGAAA
  GGAGAAGCTGCCCCAGGGCCGCAATCCTCAGCCCCAGCCCAGAGGCCCCAGCTCAAGTCC
  TGGTGGTGCCAACCCAGTGATGAAGAGGAGAGTGAGGTCAAGCCTTTGGGGGCAGCTGAG
  AAGGATGGAGAAGCTGAGTGTCCTCCCTGCATCCCCTCACCAAGTGCCTTCCTGAAGGCC
  TGGGTGTATTGGCCAGGAGAGGACACAGAGGAAGAGGAAGATGAGGAAGAAGATGAGGAC
  AGTGACTCTGGATCAGATGAGGAAGAGGGAGAAGCTGAGGCTTCCTCTTCCACTCCTGCT
  ACAGGTGTCTTCTTGAAGTCCTGGGTCTATCAGCCAGGAGAGGACACAGAGGAGGAGGAA
  GATGAGGACAGTGATACAGGATCAGCCGAGGATGAAAGAGAAGCTGAGACTTCTGCTTCC
  ACACCCCCTGCAAGTGCTTTCTTGAAGGCCTGGGTGTATCGGCCAGGAGAGGACACGGAG
  GAGGAGGAAGATGAGGATGTGGATAGTGAGGATAAGGAAGATGATTCAGAAGCAGCCTTA
  GGAGAAGCTGAGTCAGACCCACATCCCTCCCACCCGGACCAGAGTGCCCACTTCAGGGGC
  TGGGGATATCGACCTGGAAAAGAGACAGAGGAAGAGGAAGCTGCTGAGGACTGGGGAGAA
  GCTGAGCCCTGCCCCTTCCGAGTGGCCATCTATGTACCTGGAGAGAAGCCACCGCCTCCC
  TGGGCTCCTCCTAGGCTGCCCCTCCGACTGCAAAGGCGGCTCAAGCGCCCAGAAACCCCT
  ACTCATGATCCGGACCCTGAGACTCCCCTAAAGGCCAGAAAGGTGCGCTTCTCCGAGAAG
  GTCACTGTCCATTTCCTGGCTGTCTGGGCAGGGCCGGCCCAGGCCGCCCGCCAGGGCCCC
  TGGGAGCAGCTTGCTCGGGATCGCAGCCGCTTCGCACGCCGCATCGCCCAGGCCCAGGAG
  GAGCTGAGCCCCTGCCTCACCCCTGCTGCCCGGGCCAGAGCCTGGGCACGCCTCAGGAAC
  CCACCTTTAGCCCCCATCCCTGCCCTCACCCAGACCTTGCCTTCCTCCTCTGTCCCTTCG
  TCCCCAGTCCAGACCACGCCCTTGAGCCAAGCTGTGGCTACACCTTCCCGCTCCTCTGCT
  GCTGCAGCGGCTGCCCTGGACCTCAGTGGGAGGCGTGGCTGAGACCAACTGGTTTGCCTA
  TAATTTATTAACTATTTATTTTTTCTAAGTGTGGGTTTATATAAGGAATAAAGCCTTTTG
  ATTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014330 unedited
  NGTAACGTCAAATTTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGCCC
  AGTTGTTGATCTTATGCAAGACGCTGCACGACCCCGCGCCCGCTTGTCGCCACGGCACTT
  GAGGCAGCCGGAGATACTCTGAGTTACTCGGAGCCCGACGCCTGAGGGTGAGATGAACGC
  GCTGGCCTCCCTAACCGTCCGGACCTGTGATCGCTTCTGGCAGACCGAACCGGCGCTCCT
  GCCCCCGGGGTGACGCGCAGCCCCCAGCCGCCCAGACACATGGCCCCAGGCCAAGCACCC
  CATCAGGCTACCCCGTGGAGGGATGCCCACCCTTTCTTCCTCCTGTCCCCAGTGATGGGC
  CTCCTCAGCCGCGCCTGGAGCCGCCTGAGGGGCCTGGGACCTCTAGAGCCCTGGCTGGTG
  GAAGCAGTAAAAGGAGCAGCTCTGGTAGAAGCTGGCCTGGAGGGAGAAGCTAGGACTCCT
  CTGGCAATCCCCCATACCCCTTGGGGCAGACGCCCTGAAGAGGAGGCTGAAGACAGTGGA
  GGCCCTGGAGAGGACAGAGAAACACTGGGGCTGAAAACCAGCAGTTCCCTTCCTGAAGCC
  TGGGGACTTTTGGATGATGATGATGGCATGTATGGTGAGCGAGAGGCAACCAGTGTCCCT
  AGAGGGCAGGGAAGTCAATTTGCAGATGGCCAGCGTGCTCCCCTGTCTCCCAGCCTTCTG
  ATAAGGACACTGCAAGGTTCTGATAAGAACCCAGGGGAGGAGAAAGCCGAGGAAGAGGGA
  GTTGCTGAAGAGGAGGGGAGTTAACAGTTCTCTTATCCCCATCCACCGGGAGTGTTGTCC
  AGCCGTGGAGGAAGAGGACGATGAAGAAGCTGTTAAGNAAGAAGCTCACAGACCTCTACT
  TTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014330 unedited
  ACCAAGCCGCNTTCGAGATCGAGTAAATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTACAAATCAAAAGGGTTTATTCCTTATATAAACCCACACTTAAAAAAAATAAATAG
  TTAATAAATTATAGGCAAACCAGTTGGTCTCAGCCACCCCTCCCACTGAGGTCCAGGGCA
  CCCGCTGCAGCAACAAAGGAGCGGGAAGGGTGNATCCCAGCTTGGCTCAAGGGCGGGGGC
  TGGACTGGGGACCAAGGGACAAAGGAGGAAGGCAAGGTCTGGGTGAGGGCAGGGATGGGG
  GCTAAAGGGGGGTTCCTGAGGCGTGCCCAGGCTCTGGCCCGGGCAACAGGGGTGAGGCAG
  GGGCTCACCTCCTCCTGGGCCTGGGCAATGCGGCGTGCAAAGCGGCTGCAATCCCGAGCA
  AGCTGCTCCCAGGGGCCCTGGCGGGCGGCCTGGGCCGGCCCTGCCCAAACAGCCAGGAAA
  TGGACAGGGACCTTCTCGGAAAACCGCACCTTTCTGGCCCTTAGGGGAGTCTCAGGGCCC
  CGATCATGATTAGGGGTTACTGGGCCCTTGCACCGCCTTTGCCCCGGAGGGGCCCCCTCC
  CAGGAGCCCCGGGATGGGGTGGCTTTTTCTCAGGCCCCATAAAGGGCACTCCGAAGGGGG
  AGGGGTTTATCCTCCCCCCCCCCCTCTAAGCCACGCTTCCGCCCTGTCGTCTTCGCCCCG
  CCCGGCCCCGCCCCCCCCCCTAATTGGCCCCCTCTCCCCCCCCCCCGCCAGGCGATCCGC
  GACCTCTCATTATTATTCCTATTCTCCACCGCCGCCGTCCGCGTTTTGCTCCTCCGTCCC
  CATTCCCCACCGTGTCCTCTGTATCTCTATATACTCGCCCCCAAACACTATATNTAGCTA
  TATATCCTTCTTGTCCGCCGCCCGACCCAACCTCTTCTATTATAAGTCTGCAGCACCCCT
  CTCCGCCACCACATATTCACATAATAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014330
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014330.2, NP_055145.2
• RefSeq Size: 2349 bp
• RefSeq ORF: 2025 bp
• Locus ID: 23645
• UniProt ID: O75807
• Cytogenetics: 19q13.33
• Protein Families: Druggable Genome
• Gene Summary: This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008]