GADD34 (PPP1R15A) (NM_014330) Human Untagged Clone

SKU SC115045

PPP1R15A (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 15A (PPP1R15A)

Product Data

• Type: Human Untagged Clone
• Target Symbol: GADD34
• Synonyms: GADD34
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_014330 edited; GAATTCGGCACGAGGCCCAGTTGTTGATCTTATGCAAGACGCTGCACGACCCCGCGCCCG; CTTGTCGCCACGGCACTTGAGGCAGCCGGAGATACTCTGAGTTACTCGGAGCCCGACGCC; TGAGGGTGAGATGAACGCGCTGGCCTCCCTAACCGTCCGGACCTGTGATCGCTTCTGGCA; GACCGAACCGGCGCTCCTGCCCCCGGGGTGACGCGCAGCCCCCAGCCGCCCAGACACATG; GCCCCAGGCCAAGCACCCCATCAGGCTACCCCGTGGAGGGATGCCCACCCTTTCTTCCTC; CTGTCCCCAGTGATGGGCCTCCTCAGCCGCGCCTGGAGCCGCCTGAGGGGCCTGGGACCT; CTAGAGCCCTGGCTGGTGGAAGCAGTAAAAGGAGCAGCTCTGGTAGAAGCTGGCCTGGAG; GGAGAAGCTAGGACTCCTCTGGCAATCCCCCATACCCCTTGGGGCAGACGCCCTGAAGAG; GAGGCTGAAGACAGTGGAGGCCCTGGAGAGGACAGAGAAACACTGGGGCTGAAAACCAGC; AGTTCCCTTCCTGAAGCCTGGGGACTTTTGGATGATGATGATGGCATGTATGGTGAGCGA; GAGGCAACCAGTGTCCCTAGAGGGCAGGGAAGTCAATTTGCAGATGGCCAGCGTGCTCCC; CTGTCTCCCAGCCTTCTGATAAGGACACTGCAAGGTTCTGATAAGAACCCAGGGGAGGAG; AAAGCCGAGGAAGAGGGAGTTGCTGAAGAGGAGGGAGTTAACAAGTTCTCTTATCCACCA; TCACACCGGGAGTGTTGTCCAGCCGTGGAGGAGGAGGACGATGAAGAAGCTGTAAAGAAA; GAAGCTCACAGAACCTCTACTTCTGCCTTGTCTCCAGGATCCAAGCCCAGCACTTGGGTG; TCTTGCCCAGGGGAGGAAGAGAATCAAGCCACGGAGGATAAAAGAACAGAAAGAAGTAAA; GGAGCCAGGAAGACCTCCGTGTCCCCCCGATCTTCAGGCTCCGACCCCAGGTCCTGGGAG; TATCGTTCAGGAGAGGCGTCCGAGGAGAAGGAGGAAAAGGCACACGAAGAAACTGGGAAA; GGAGAAGCTGCCCCAGGGCCGCAATCCTCAGCCCCAGCCCAGAGGCCCCAGCTCAAGTCC; TGGTGGTGCCAACCCAGTGATGAAGAGGAGAGTGAGGTCAAGCCTTTGGGGGCAGCTGAG; AAGGATGGAGAAGCTGAGTGTCCTCCCTGCATCCCCTCACCAAGTGCCTTCCTGAAGGCC; TGGGTGTATTGGCCAGGAGAGGACACAGAGGAAGAGGAAGATGAGGAAGAAGATGAGGAC; AGTGACTCTGGATCAGATGAGGAAGAGGGAGAAGCTGAGGCTTCCTCTTCCACTCCTGCT; ACAGGTGTCTTCTTGAAGTCCTGGGTCTATCAGCCAGGAGAGGACACAGAGGAGGAGGAA; GATGAGGACAGTGATACAGGATCAGCCGAGGATGAAAGAGAAGCTGAGACTTCTGCTTCC; ACACCCCCTGCAAGTGCTTTCTTGAAGGCCTGGGTGTATCGGCCAGGAGAGGACACGGAG; GAGGAGGAAGATGAGGATGTGGATAGTGAGGATAAGGAAGATGATTCAGAAGCAGCCTTA; GGAGAAGCTGAGTCAGACCCACATCCCTCCCACCCGGACCAGAGTGCCCACTTCAGGGGC; TGGGGATATCGACCTGGAAAAGAGACAGAGGAAGAGGAAGCTGCTGAGGACTGGGGAGAA; GCTGAGCCCTGCCCCTTCCGAGTGGCCATCTATGTACCTGGAGAGAAGCCACCGCCTCCC; TGGGCTCCTCCTAGGCTGCCCCTCCGACTGCAAAGGCGGCTCAAGCGCCCAGAAACCCCT; ACTCATGATCCGGACCCTGAGACTCCCCTAAAGGCCAGAAAGGTGCGCTTCTCCGAGAAG; GTCACTGTCCATTTCCTGGCTGTCTGGGCAGGGCCGGCCCAGGCCGCCCGCCAGGGCCCC; TGGGAGCAGCTTGCTCGGGATCGCAGCCGCTTCGCACGCCGCATCGCCCAGGCCCAGGAG; GAGCTGAGCCCCTGCCTCACCCCTGCTGCCCGGGCCAGAGCCTGGGCACGCCTCAGGAAC; CCACCTTTAGCCCCCATCCCTGCCCTCACCCAGACCTTGCCTTCCTCCTCTGTCCCTTCG; TCCCCAGTCCAGACCACGCCCTTGAGCCAAGCTGTGGCTACACCTTCCCGCTCCTCTGCT; GCTGCAGCGGCTGCCCTGGACCTCAGTGGGAGGCGTGGCTGAGACCAACTGGTTTGCCTA; TAATTTATTAACTATTTATTTTTTCTAAGTGTGGGTTTATATAAGGAATAAAGCCTTTTG; ATTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014330 unedited; NGTAACGTCAAATTTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGCCC; AGTTGTTGATCTTATGCAAGACGCTGCACGACCCCGCGCCCGCTTGTCGCCACGGCACTT; GAGGCAGCCGGAGATACTCTGAGTTACTCGGAGCCCGACGCCTGAGGGTGAGATGAACGC; GCTGGCCTCCCTAACCGTCCGGACCTGTGATCGCTTCTGGCAGACCGAACCGGCGCTCCT; GCCCCCGGGGTGACGCGCAGCCCCCAGCCGCCCAGACACATGGCCCCAGGCCAAGCACCC; CATCAGGCTACCCCGTGGAGGGATGCCCACCCTTTCTTCCTCCTGTCCCCAGTGATGGGC; CTCCTCAGCCGCGCCTGGAGCCGCCTGAGGGGCCTGGGACCTCTAGAGCCCTGGCTGGTG; GAAGCAGTAAAAGGAGCAGCTCTGGTAGAAGCTGGCCTGGAGGGAGAAGCTAGGACTCCT; CTGGCAATCCCCCATACCCCTTGGGGCAGACGCCCTGAAGAGGAGGCTGAAGACAGTGGA; GGCCCTGGAGAGGACAGAGAAACACTGGGGCTGAAAACCAGCAGTTCCCTTCCTGAAGCC; TGGGGACTTTTGGATGATGATGATGGCATGTATGGTGAGCGAGAGGCAACCAGTGTCCCT; AGAGGGCAGGGAAGTCAATTTGCAGATGGCCAGCGTGCTCCCCTGTCTCCCAGCCTTCTG; ATAAGGACACTGCAAGGTTCTGATAAGAACCCAGGGGAGGAGAAAGCCGAGGAAGAGGGA; GTTGCTGAAGAGGAGGGGAGTTAACAGTTCTCTTATCCCCATCCACCGGGAGTGTTGTCC; AGCCGTGGAGGAAGAGGACGATGAAGAAGCTGTTAAGNAAGAAGCTCACAGACCTCTACT; TTC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014330 unedited; ACCAAGCCGCNTTCGAGATCGAGTAAATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTACAAATCAAAAGGGTTTATTCCTTATATAAACCCACACTTAAAAAAAATAAATAG; TTAATAAATTATAGGCAAACCAGTTGGTCTCAGCCACCCCTCCCACTGAGGTCCAGGGCA; CCCGCTGCAGCAACAAAGGAGCGGGAAGGGTGNATCCCAGCTTGGCTCAAGGGCGGGGGC; TGGACTGGGGACCAAGGGACAAAGGAGGAAGGCAAGGTCTGGGTGAGGGCAGGGATGGGG; GCTAAAGGGGGGTTCCTGAGGCGTGCCCAGGCTCTGGCCCGGGCAACAGGGGTGAGGCAG; GGGCTCACCTCCTCCTGGGCCTGGGCAATGCGGCGTGCAAAGCGGCTGCAATCCCGAGCA; AGCTGCTCCCAGGGGCCCTGGCGGGCGGCCTGGGCCGGCCCTGCCCAAACAGCCAGGAAA; TGGACAGGGACCTTCTCGGAAAACCGCACCTTTCTGGCCCTTAGGGGAGTCTCAGGGCCC; CGATCATGATTAGGGGTTACTGGGCCCTTGCACCGCCTTTGCCCCGGAGGGGCCCCCTCC; CAGGAGCCCCGGGATGGGGTGGCTTTTTCTCAGGCCCCATAAAGGGCACTCCGAAGGGGG; AGGGGTTTATCCTCCCCCCCCCCCTCTAAGCCACGCTTCCGCCCTGTCGTCTTCGCCCCG; CCCGGCCCCGCCCCCCCCCCTAATTGGCCCCCTCTCCCCCCCCCCCGCCAGGCGATCCGC; GACCTCTCATTATTATTCCTATTCTCCACCGCCGCCGTCCGCGTTTTGCTCCTCCGTCCC; CATTCCCCACCGTGTCCTCTGTATCTCTATATACTCGCCCCCAAACACTATATNTAGCTA; TATATCCTTCTTGTCCGCCGCCCGACCCAACCTCTTCTATTATAAGTCTGCAGCACCCCT; CTCCGCCACCACATATTCACATAATAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_014330
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014330.2, NP_055145.2
• RefSeq Size: 2349 bp
• RefSeq ORF: 2025 bp
• Locus ID: 23645
• UniProt ID: O75807
• Cytogenetics: 19q13.33
• Protein Families: Druggable Genome
• Summary: This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008]