Rabex5 (RABGEF1) (NM_014504) Human Untagged Clone

SKU SC114993

RABGEF1 (untagged)-Human RAB guanine nucleotide exchange factor (GEF) 1 (RABGEF1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Rabex5
• Synonyms: rabex-5; RABEX5; RAP1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114993 sequence for NM_014504 edited (data generated by NextGen Sequencing); ATGAGCCTTAAGTCTGAACGCCGAGGAATTCATGTGGATCAATCGGATCTCCTGTGCAAG; AAAGGATGTGGTTACTACGGCAACCCTGCCTGGCAGGGTTTCTGCTCCAAGTGCTGGAGG; GAAGAGTACCACAAAGCCAGGCAGAAGCAGATTCAGGAGGACTGGGAGCTGGCGGAGCGA; CTCCAGCGGGAGGAAGAAGAGGCCTTTGCCAGCAGTCAGAGCAGCCAAGGGGCCCAATCC; CTCACATTCTCCAAGTTTGAAGAAAAGAAAACCAACGAGAAGACCCGCAAGGTTACCACA; GTGAAGAAATTCTTCAGTGCATCTTCCAGGGTCGGATCAAAGAAGGAAATTCAGGAAGCA; AAAGCTCCCAGTCCTTCCATAAACCGGCAAACCAGCATTGAAACGGATAGAGTGTCTAAG; GAGTTCATAGAATTTCTCAAGACCTTCCACAAGACAGGCCAAGAAATCTATAAACAGACC; AAGCTGTTTTTGGAAGGAATGCATTACAAAAGGGATCTAAGCATTGAAGAACAGTCAGAG; TGTGCTCAGGATTTCTACCACAATGTGGCCGAAAGGATGCAAACTCGTGGGAAAGTGCCT; CCAGAAAGAGTCGAGAAGATAATGGATCAGATTGAAAAGTACATCATGACTCGTCTCTAT; AAATATGTATTCTGTCCAGAAACTACTGATGATGAGAAGAAAGATCTTGCCATTCAAAAG; AGAATCAGAGCCCTGCGCTGGGTTACGCCTCAGATGCTGTGTGTCCCTGTTAATGAAGAC; ATCCCAGAAGTGTCTGATATGGTGGTGAAGGCGATCACAGATATCATTGAAATGGATTCC; AAGCGTGTGCCTCGAGACAAGCTGGCCTGCATCACCAAGTGCAGCAAGCACATCTTCAAT; GCCATCAAGATCACCAAGAATGAGCCGGCGTCAGCGGATGACTTCCTCCCCACCCTCATC; TACATTGTTTTGAAGGGCAACCCCCCACGCCTTCAGTCTAATATCCAGTATATCACGCGC; TTCTGCAATCCAAGCCGACTGATGACTGGAGAGGATGGCTACTATTTCACCAATCTGTGC; TGTGCTGTGGCTTTCATTGAGAAGCTAGACGCCCAGTCTTTGAATCTAAGTCAGGAGGAT; TTTGATCGCTACATGTCTGGCCAGACCTCTCCCAGGAAGCAAGAAGCTGAGAGTTGGTCT; CCTGATGCTTGCTTAGGCGTCAAGCAAATGTATAAGAACTTGGATCTCTTGTCTCAGTTG; AATGAACGACAAGAAAGGATCATGAATGAAGCCAAGAAACTGGAAAAAGACCTCATAGAT; TGGACAGATGGAATTGCAAGAGAAGTTCAAGACATCGTTGAGAAATACCCACTGGAAATT; AAGCCTCCGAATCAACCGTTAGCAGCTATTGACTCTGAAAACGTTGAAAATGATAAACTT; CCTCCACCACTGCAACCTCAAGTTTATGCAGGATGA; ; Clone variation with respect to NM_014504.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014504 unedited; GTTCAGGAATTTGTAATACGACTCACTATAGGGCGGCCGCGCATTCGGCACGAGGCTGGC; CGCGGAGCCCAGACCTACCCGGGCGAAGCGGGCGAGCGGTGGTTTGGACGCCGGCGGAGA; CGCGGGCGAGTGGTTAGCAGGAAGAAGATGAGCCTTAAGTCTGAACGCCGAGGAATTCAT; GTGGATCAATCGGATCTCCTGTGCAAGAAAGGATGTGGTTACTACGGCAACCCTGCCTGG; CAGGGTTTCTGCTCCAAGTGCTGGAGGGAAGAGTACCACAAAGCCAGGCAGAAGCAGATT; CAGGAGGACTGGGAGCTGGCGGAGCGACTCCAGCGGGAGGAAGAAGAGGCCTTTGCCAGC; AGTCAGAGCAGCCAAGGGGCCCAATCCCTCACATTCTCCAAGTTTGAAGAAAAGAAAACC; AACGAGAAGACCCGCAAGGTTACCACAGTGAAGAAATTCTTCAGTGCATCTTCCAGGGTC; GGATCAAAGAAGGAAATTCAGGAAGCAAAAGCTCCCAGTCCTTCCATAAACCGGCAAACC; AGCATTGAAACGGATAGAGTGTCTAAGGAGTTCATAGAATTTCTCAAGACCTTCCACAAG; ACAGGCCAAGAAATCTATAAACAGACCAAGCTGTTTTTGGAAGGAATGCATTACAAAAGG; GATCTAAGCATTGAAGAACAGTCAGAGTGTGCTCAGGATTTCTACCACAATGTGGCCGAA; AGGATGCAAACTCGTGGGAAAGTGCCTCCAGAAAGAGTCGAGAAGATAATGGATCAGATT; GAAAAGTACATCATGACTCGTCTCTATAATATGTATTCTGTCCAGAACCTACTGATGATG; AGAAGAAAGATCTTGCCATTCAAAAGAGATCAGAGCCCTGCGCTGGGTTACGCCTCAGAT; GCTGTGTGTCCCTGTTATGAAGACATCCACAAATGTCTGATT;
• Restriction Sites: NotI-NotI
• ACCN: NM_014504
• Insert Size: 4130 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014504.1, NP_055319.1
• RefSeq Size: 3740 bp
• RefSeq ORF: 1476 bp
• Locus ID: 27342
• UniProt ID: Q9UJ41
• Cytogenetics: 7q11.21
• Domains: VPS9, zf-A20
• Summary: RABGEF1 forms a complex with rabaptin-5 (RABPT5; MIM 603616) that is required for endocytic membrane fusion, and it serves as a specific guanine nucleotide exchange factor (GEF) for RAB5 (RAB5A; MIM 179512) (Horiuchi et al., 1997 [PubMed 9323142]).[supplied by OMIM, Mar 2010]; Transcript Variant: This variant (4) contains alternate 5' exon structure and includes an additional internal exon, and it thus differs in its 5' UTR and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (c) is longer at the N-terminus, compared to isoform a. Variants 3, 4, and 9-20 all encode the same isoform (c). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.