HIV TAT specific factor 1 (HTATSF1) (NM_014500) Human Untagged Clone

SKU SC114990

HTATSF1 (untagged)-Human HIV-1 Tat specific factor 1 (HTATSF1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: HIV TAT specific factor 1
• Synonyms: dJ196E23.2; TAT-SF1; TATSF1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114990 sequence for NM_014500 edited (data generated by NextGen Sequencing); ATGAGCGGCACCAACTTGGATGGGAACGATGAGTTTGATGAGCAGTTGCGAATGCAAGAA; TTGTACGGAGACGGCAAGGATGGTGACACCCAGACCGATGCCGGCGGAGAACCCGATTCT; CTCGGGCAGCAGCCGACGGACACTCCCTACGAGTGGGACCTGGACAAAAAGGCTTGGTTC; CCCAAGATTACTGAAGATTTCATTGCTACATATCAGGCCAATTATGGCTTCTCTAACGAT; GGCGCATCTAGTTCTACCGCAAATGTTGAAGATGTCCATGCTAGGACTGCAGAGGAACCT; CCACAAGAAAAAGCCCCGGAACCCACTGATGCCAGAAAGAAGGGAGAAAAAAGAAAGGCT; GAGTCAGGATGGTTTCATGTTGAAGAAGACAGAAATACAAATGTATACGTGTCTGGTTTG; CCTCCAGATATTACAGTGGATGAATTTATACAACTTATGTCCAAGTTTGGCATTATTATG; AGAGATCCTCAGACAGAAGAATTTAAGGTCAAACTTTACAAAGATAATCAAGGAAATCTT; AAAGGAGACGGTCTTTGCTGTTATTTGAAAAGAGAATCTGTGGAACTTGCATTAAAACTT; TTGGATGAAGATGAAATTAGAGGCTACAAATTACATGTTGAGGTGGCAAAGTTTCAACTG; AAGGGAGAATATGATGCCTCAAAGAAGAAGAAGAAGTGCAAAGACTATAAGAAGAAGCTG; TCTATGCAACAAAAGCAGTTGGATTGGAGACCTGAGAGGCGAGCCGGACCATCCCGGATG; CGCCATGAGCGAGTTGTCATCATCAAGAATATGTTTCATCCTATGGATTTTGAGGATGAT; CCGTTGGTGCTGAATGAGATCAGAGAAGACCTTCGAGTAGAGTGTTCGAAGTTTGGACAA; ATTAGGAAACTCCTTCTCTTTGATAGGCACCCAGATGGTGTGGCCTCTGTGTCCTTTCGG; GATCCAGAGGAAGCTGATTATTGTATTCAGACTCTCGATGGAAGATGGTTTGGTGGCCGT; CAAATCACTGCCCAGGCATGGGATGGGACTACAGATTATCAGGTGGAGGAAACCTCAAGA; GAAAGGGAGGAAAGGCTGAGAGGATGGGAGGCTTTCCTCAATGCTCCTGAGGCCAACAGA; GGCCTTAGGCGTTCAGATTCTGTCTCTGCTTCCGAAAGGGCAGGGCCTTCTAGAGCAAGG; CATTTTTCAGAGCACCCCAGCACATCTAAAATGAATGCTCAAGAAACTGCAACTGGAATG; GCGTTTGAAGAACCTATAGATGAGAAGAAGTTTGAAAAGACAGAAGATGGGGGAGAATTT; GAAGAAGGTGCTTCTGAAAACAATGCTAAGGAAAGTAGCCCCGAAAAAGAGGCTGAAGAA; GGCTGCCCTGAAAAAGAATCTGAAGAGGGCTGCCCCAAAAGAGGGTTTGAAGGCAGCTGC; TCCCAAAAAGAGTCTGAAGAAGGCAATCCCGTAAGAGGATCTGAAGAGGATAGTCCTAAA; AAAGAGTCTAAAAAGAAGACACTCAAAAATGATTGTGAAGAGAATGGCCTTGCAAAGGAA; TCTGAAGATGACCTCAACAAGGAGTCTGAAGAGGAGGTTGGCCCCACAAAAGAGTCCGAA; GAAGATGACTCAGAGAAAGAGTCTGATGAAGACTGCTCTGAAAAACAGTCTGAAGATGGC; TCCGAAAGAGAATTTGAAGAAAATGGTCTCGAGAAAGATTTGGATGAGGAAGGTTCTGAA; AAGGAGCTTCATGAAAATGTTCTTGACAAAGAGTTAGAAGAAAATGACTCTGAAAACTCC; GAATTTGAAGATGACGGCTCTGAAAAAGTGTTAGATGAGGAAGGCTCTGAGAGAGAGTTT; GACGAAGATTCAGATGAAAAGGAAGAAGAGGAGGATACATATGAAAAAGTATTTGATGAT; GAGTCTGATGAGAAAGAGGATGAAGAATATGCAGATGAAAAGGGGCTTGAAGCTGCTGAT; AAAAAGGCGGAAGAAGGTGATGCAGATGAAAAGCTGTTTGAAGAGTCAGATGACAAGGAA; GATGAAGATGCAGATGGAAAGGAAGTTGAAGATGCTGACGAAAAGTTGTTCGAAGATGAT; GATTCCAATGAGAAGTTGTTTGATGAGGAGGAAGATTCCAGTGAGAAGTTGTTTGACGAT; TCTGATGAGAGGGGGACTTTGGGTGGTTTTGGGAGTGTTGAAGAAGGGCCCCTATCCACT; GGCAGCAGCTTTATTCTCAGTAGCGATGATGATGACGATGATATTTAA; ; Clone variation with respect to NM_014500.4; 1725 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014500 unedited; TATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGAAACATGAGCGGCACCAACT; TGGATGGGAACGATGAGTTTGATGAGCAGTTGCGAATGCAAGAATTGTACGGAGACGGCA; AGGATGGTGACACCCAGACCGATGCCGGCGGAGAACCCGATTCTCTCGGGCAGCAGCCGA; CGGACACTCCCTACGAGTGGGACCTGGACAAAAAGGCTTGGTTCCCCAAGATTACTGAAG; ATTTCATTGCTACATATCAGGCCAATTATGGCTTCTCTAACGATGGCGCATCTAGTTCTA; CCGCAAATGTTGAAGATGTCCATGCTAGGACTGCAGAGGAACCTCCACAAGAAAAAGCCC; CGGAACCCACTGATGCCAGAAAGAAGGGAGAAAAAAGAAAGGCTGAGTCAGGATGGTTTC; ATGTTGAAGAAGACAGAAATACAAATGTATACGTGTCTGGTTTGCCTCCAGATATTACAG; TGGATGAATTTATACAACTTATGTCCAAGTTTGGCATTATTATGAGAGATCCTCAGACAG; AAGAATTTAAGGTCAAACTTTACAAAGATAATCAAGGAAATCTTAAAGGAGACGGTCTTT; GCTGTTATTTGAAAAGAGAATCTGTGGAACTTGCATTANAACTTTTGGATGAAGATGAAA; TTAGAGGCTANCAATTACATGGTTGAGGTGGNCAAAGTTTCAACTGAAGGGAGAATATTG; ATGCCTCNAAGAGAAGAANGAAGTGCAAGACTATAGNAGAAAGCTGTCTATGAAACAAAA; GCAGTTGATGGNAGACCTGAAAGCGAGCCGGACCATCCGGNATGCGCATGAGACGATTGN; CATATCAGAAATTGGTTCATCTTAGGATTGAGGAAAACCCCTGGTCTGAATGAACAAAAA; AACCTTGAAGNAAAGGTNCAAGTTGGACAATTGGAACTTCCTCTTGATAGCCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014500 unedited; GGGTTTCCTCCCCTTTTCTTTTATTGTCGCAACCCCACATACTTTACTCACTCCCCTCAA; AATATTATTGTGCATGCATCTATCAAATTTGACACAAGCACAAGTCTCCAGTTTACTTGC; ATTGTAACTATGACATTGTAACTAAACAATTAATTAACGTATATTCAAAACCATTATGTA; CAGGTAACAATGAAACACTTGAATGGTCTAATGTGATGGCATACTACCACTATGCACATG; TCGCATGATAACGACTAGCTAGTTGATCTGATCCCGTGCAATGCACTATGGGCGACATGT; CGACACGTGGAGGCACCTCTAAGCTTCACATCCCCATGCGTTCATCCGTGACCATATATT; TCGCCCCGTCCATTGTATCATCCCGATTCCTTGATAATGCAGCTCTGTCCGACCATGTGT; CATCCCGGTGCCTCTTGCTTGTAACACTCCCGGAGAAACGTCGCTTACGGCGTTTCTGTC; CGCCGAGACTTGTCCCCGCGTCGTCGGTCGACTGCGACTGTTCGCGCCTATTCCGCCTCA; ACCGTCAGTTCGGCGGCCGACTGTTTGTATGCGTATCACTATGTTCACCTTCACCATCGC; GCCGGCCCCCCTATCTCCGACATAATATTATTTAACTTAGATTAATAGTCCGTCGCACCG; TATGAAGTCTGTGATTGTTGGTCGCCTTTTGCGCCATCCCGTCTCGCGCAGCCGTATCCC; ATTCCATATTACGTTTTATTTTTTGTTCCTTTCGTCCGTCCATGTGATCCCCCGCTTCCC; GCCCTTTTCTTCCCTCTTTTCCGTTATCCGTTCGTCCCGTCTTTCCATGTGTATCCCGTG; CCGCGCTCTTCGCTCTTCCCATTTTATTCGTTCCCGCTCCCCCGCCCCTTACGCATACCC; GTATGTCCGCTCTCCCTNCTCCGAGTCCGTGTTCGTCTCCGACCCCGCAGTGTTTTGCAC; CCCTTCGCTCCCGTCGTTCCCCGATCCCTCGTTGTATCAACCGAACCGTGCTNCCACCTT; TGATCCGCTTCGCATTGTCTCCTGTTCTTTGCCGCCTCGCGCGACGTCCTCTGTTCACGC; GTGGTCGCTCNATACGTCGCCGCCCGTCTCTGCGATGTCGGTATCTCACCATCCCTCTCC; ATTATAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_014500
• Insert Size: 2840 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014500.3, NP_055315.2
• RefSeq Size: 2776 bp
• RefSeq ORF: 2268 bp
• Locus ID: 27336
• UniProt ID: O43719
• Cytogenetics: Xq26.3
• Domains: RRM
• Protein Families: Transcription Factors
• Summary: The protein encoded by this gene functions as a cofactor for the stimulation of transcriptional elongation by HIV-1 Tat, which binds to the HIV-1 promoter through Tat-TAR interaction. This protein may also serve as a dual-function factor to couple transcription and splicing and to facilitate their reciprocal activation. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Sep 2009]; Transcript Variant: This variant (2) is missing a 5' non-coding exon, and therefore, has a different 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.