Centaurin gamma 1 (AGAP2) (NM_014770) Human Untagged Clone

CAT#: SC114863

AGAP2 (untagged)-Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Centaurin gamma 1
• Synonyms: CENTG1; GGAP2; PIKE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_014770, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCATGCCCAGAGGCAGTTCGTTGTAGCTGCAGTGAGAGCAGAAGTCAGACGACATGAGGTGGCCAAGC
  AGGCTCTAAACCGCCTCAGGAAGCTGGCAGAGAGGGTGGACGACCCCGAACTCCAGGACAGCATCCAGGC
  CTCATTGGACAGCATTCGAGAGGCTGTGATCAATAGCCAGGAATGGACTTTGAGCCGCTCCATTCCTGAA
  CTGCGCCTGGGTGTGCTGGGCGATGCCAGGAGTGGGAAGTCATCGCTCATCCACCGATTCCTGACTGGCT
  CATACCAGGTGCTGGAGAAGACAGAGAGTGAGCAGTACAAGAAAGAAATGTTGGTGGATGGACAGACACA
  TCTGGTGCTAATCCGAGAGGAAGCTGGGGCACCTGATGCCAAGTTCTCAGGCTGGGCAGATGCTGTGATC
  TTCGTCTTCAGCCTGGAGGATGAGAACAGTTTCCAGGCTGTGAGCCGTCTCCATGGGCAGCTGAGTTCCC
  TTCGCGGGGAGGGACGAGGAGGCCTGGCCTTGGCACTGGTGGGGACACAAGACAGGATCAGTGCTTCCTC
  CCCTCGGGTGGTGGGAGATGCTCGTGCCAGAGCTCTGTGCGCGGACATGAAACGCTGCAGCTACTATGAG
  ACTTGTGCAACCTATGGGCTCAATGTGGATCGGGTCTTCCAGGAGGTGGCCCAGAAGGTGGTGACCTTGC
  GCAAGCAGCAACAGCTTCTGGCTGCCTGCAAGTCCCTGCCCAGCTCCCCAAGCCACTCAGCTGCATCCAC
  TCCGGTAGCTGGCCAGGCTAGTAACGGGGGCCACACTAGCGACTACTCTTCTTCCCTCCCGTCCTCACCG
  AATGTTGGTCACCGGGAGCTCCGAGCCGAGGCAGCTGCAGTGGCTGGATTGAGCACCCCAGGGTCCCTGC
  ACCGGGCAGCCAAGCGCAGGACCAGCCTTTTTGCGAATCGTCGGGGTAGTGACTCCGAGAAACGAAGCTT
  GGATAGTCGGGGAGAGACAACAGGGAGTGGGCGAGCCATCCCCATCAAACAGAGCTTCCTACTAAAACGA
  AGTGGCAATTCCTTGAACAAAGAATGGAAGAAGAAATATGTAACCCTGTCCAGTAATGGCTTTCTACTCT
  ACCACCCCAGTATTAACGATTACATCCACAGTACCCACGGCAAGGAGATGGACTTGCTGCGAACAACAGT
  CAAAGTCCCGGGCAAGCGGCCCCCGAGGGCCATCTCTGCCTTTGGCCCCTCAGCCAGCATTAACGGGCTC
  GTCAAGGACATGAGCACTGTCCAGATGGGTGAAGGCCTGGAAGCCACTACTCCCATGCCAAGCCCTAGCC
  CCAGCCCCAGTTCCCTGCAGCCACCACCAGATCAGACATCCAAACACCTGCTGAAGCCAGACCGGAATTT
  GGCCCGAGCCCTCAGCACGGACTGTACCCCATCTGGAGACCTGAGCCCCCTGAGTCGGGAACCCCCTCCT
  TCTCCCATGGTGAAGAAGCAGAGGAGGAAAAAATTGACAACACCATCCAAGACTGAAGGCTCGGCTGGGC
  AGGCTGAAGAGGAAAACTTTGAGTTCCTGATCGTGTCCAGCACGGGTCAGACGTGGCACTTTGAGGCAGC
  CAGTTTTGAGGAGCGGGATGCCTGGGTCCAGGCCATCGAGAGTCAGATCCTAGCCAGTCTGCAATGCTGT
  GAGAGCAGCAAGGTCAAGCTGCGCACAGACAGCCAAAGCGAGGCCGTGGCCATCCAGGCGATCCGGAACG
  CCAAGGGGAATTCAATCTGCGTGGACTGCGGGGCCCCCAACCCCACGTGGGCCAGCTTGAACCTGGGCGC
  CCTCATCTGCATCGAGTGTTCTGGCATCCACCGCAACCTGGGCACACACCTGTCCCGCGTTCGCTCGCTG
  GACTTGGACGACTGGCCACGGGAGCTGACCCTGGTGCTGACGGCTATTGGCAACGACACGGCCAACCGCG
  TGTGGGAAAGCGACACGCGAGGCCGTGCCAAGCCCTCGCGGGACTCTTCGCGGGAGGAGCGCGAGTCGTG
  GATTCGCGCCAAGTACGAGCAGCTACTGTTCCTGGCGCCGCTGAGCACCTCGGAGGAGCCGCTGGGCCGC
  CAGCTGTGGGCCGCCGTGCAGGCCCAGGACGTGGCTACCGTTCTCCTGCTTTTGGCCCATGCGCGACACG
  GGCCGCTCGACACCAGCGTAGAGGACCCACAGCTGCGCTCCCCACTCCACCTGGCGGCCGAGCTCGCCCA
  CGTCGTCATCACGCAACTGCTGCTGTGGTACGGCGCGGACGTGGCGGCCCGTGACGCCCAGGGCCGCACG
  GCGCTGTTCTACGCCCGCCAGGCTGGAAGCCAGCTGTGCGCCGACATCCTTCTCCAGCACGGCTGCCCGG
  GTGAGGGCGGCAGCGCGGCCACCACGCCCAGCGCGGCCACCACGCCCAGCATCACCGCCACGCCCAGCCC
  CCGCCGCCGGAGCAGCGCCGCTAGCGTGGGCCGCGCCGACGCCCCGGTTGCGCTGGTATAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014770 unedited
  NTTTTTTGGAAATANATAAAAATAAAAAAANNNNTTTTCCCCCGCCCGNTGCCGCAAAGG
  GCGGTAGGCGTGTACGGTGGGNAGTCTATATAAGCAGAGCTCATTTAGGTGACACTATAG
  AATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGGCAAGAGTAAG
  ACCTTGGACAACAGTGACTTGCATCCGGGACCGCCTGCCGGCTCTCCTCCTCCGCTAACC
  CTCCCACCAACTCCGAGTCCAGCCACTGCTGTCACCGCTGCTTCCGCGCAGCCCCCCGGG
  CCTGCACCTCCAATCACTCTGGAGCCTCCAGCTCCGGGGCTGAAACGGGGCCGGGAGGGG
  GGCCGAGCATCCACTCGTGACCGCAAGATGCTCAAGTTTATCAGCGGCATCTTCACCAAG
  AGCACAGGAGGGCCTCCTGGCTCCGGGCCCCTTCCCGGACCCCCCAGCCTGTCTTCTGGC
  AGCGGGTCCAGGGAGCTGCTGGGCGCCGAGCTCCGCGCTTCCCCTAAGGCTGTGATCAAT
  AGCCAGGAATGGACTTTGAGCCGCTCCATTCCTGAACTGCGCCTGGGTGTGCTGGGCGAT
  GCCAGGAGTGGGAAGTCATCGCTCATCCACCGATTCCTGACTGGCTCATACCAGGTGCTG
  GAGAAGACAGAGAGTGAGCAGTACAAGAAAGAAATGTTGGTGGATGGACAGACACATCTG
  GTGCTAATCCGAGAGGAAGCTGGGGCACCTGATGCCAAGTTCTCAGGCTGGGCAGATGCT
  GTGATCTTCGTCTTCAGCCTGGGAGATGAGAACAGTTTCCAGGCTGTGAGCCGTCTCCAT
  GGGCAGCTGAGTTCCCTTCGCGGGGAGGGACCGAGGAGCCTGGCCCGGNCACTGGTGGNG
  ACACCAGACGGATCAGTGCTTNCTCCCCTCGGGTGGTAGGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014770 unedited
  CCATTGCCCCTTGGGTGATGGCACTTCCCAGGNCCAGNGNGAGCACTGGGGAAGGGAGTC
  ACAGGGCATGCCACCCGGGTTCTGTTCAGGAAAAGCTATGACCGCGGCCGCAATCTAGAG
  TCGAGTTTTTTTTTTTTTTTTTTGTGGAAACAATGGAGAGGGGGCGTGTTGAGCTGGGGT
  CTCCATGCCTCGTTGGGGAGAGGGAGGTGAGTTTGTGTCTTCTGGAAGGCGTGGGGGCTG
  TGCCCTCGTGGGGGTAGGAAGTGCTCCCGTGGGGCGGGGTGCGGATCGGAGAGGTGAGTG
  GGTGCGTCTGTCCAGCGGTCAGCCCGGTGTGGTCGTGCCCGGCCCGCGTGGGGATGGGGG
  TGTCTCTCCCGCTGGGCAACTATACCAGCGCAACCGGGGCGTCGGCGCGGCCCACGCTAG
  CGGCGCTGCTCCGGCGGCGGGGGCTGGGCGTGGCGGTGATGCTGGGCGTGGTGGCCGCGC
  TGGGCGTGGTGGCCGCGCTGCCGCCCTCACCCGGGCAGCCGTGCTGGAGAAGGATGTCGG
  CGCACAGCTGGCTTCCAGCCTGGCGGGCGTAGAACAGCGCCGTGCGGCCCTGGGCGTCAC
  GGGCCGCCACGTCCGCGCCGTACCACAGAAGCAGTTGCGTGATGACGACGTGCGCTGAGC
  TCGGCCGCCACGTGGAGTGTGGAGCGCAGCTGTGGGTCCTCTACGCTGGTGTCGAACGGG
  CCGTGTAGCGCATGGGCCAAAAACCAGATCACGAAATCACCGTCCTGGGCCTGCACTGGG
  GCCCCAAGCTGGGGGCTCAATCGGCTTCTCCGAGGTGCTAAGAGGCCCCGGGAATATTTC
  CTGTTCCGTATTGGCGCGAAACCATAATTCGTGCTCCTCCGGCAATTATTCCGCCAAGGC
  T
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014770
• Insert Size: 3250 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014770.2, NP_055585.1
• RefSeq Size: 3938 bp
• RefSeq ORF: 2511 bp
• Locus ID: 116986
• UniProt ID: Q99490
• Cytogenetics: 12q14.1
• Domains: ArfGap, PH, ANK, RAS, RAB
• Protein Pathways: Endocytosis
• Gene Summary: The protein encoded by this gene belongs to the centaurin gamma-like family. It mediates anti-apoptotic effects of nerve growth factor by activating nuclear phosphoinositide 3-kinase. It is overexpressed in cancer cells, and promotes cancer cell invasion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]
  Transcript Variant: This variant (2) contains an alternate 5' terminal exon compared to variant 1. This results in a shorter isoform (PIKE-A) with a distinct N-terminus compared to isoform PIKE-L. This isoform is reported to be overexpressed in brain tumors and promote cancer cell invasion (PMID:14761976).