BTF (BCLAF1) (NM_014739) Human Untagged Clone

SKU SC114841

BCLAF1 (untagged)-Human BCL2-associated transcription factor 1 (BCLAF1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: BTF
• Synonyms: bK211L9.1; BTF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114841 sequence for NM_014739 edited (data generated by NextGen Sequencing); ATGGGTCGCTCCAATTCTAGATCACATTCTTCAAGGTCAAAGTCTAGATCACAGTCTAGT; TCTCGATCAAGATCAAGATCTCATTCTAGAAAGAAGCGATACAGTTCTAGGTCTCGTTCC; AGAACATATTCAAGGTCTCGTAGTAGAGATCGTATGTATTCTAGAGATTATCGTCGCGAT; TACAGAAATAATAGAGGAATGAGACGACCTTATGGGTACAGAGGAAGGGGTAGAGGGTAT; TATCAAGGAGGAGGAGGTAGATATCATCGAGGTGGTTATAGACCTGTCTGGAATAGAAGG; CACTCTAGGAGTCCTAGACGAGGTCGTTCACGTTCCAGGAGTCCAAAAAGAAGATCCGTT; TCTTCTCAAAGATCCAGAAGCAGATCTCGCCGGTCATATAGATCTTCTAGGTCTCCAAGA; TCATCCTCTTCTCGTTCTTCATCCCCATATAGCAAATCTCCTGTTTCTAAAAGACGAGGG; TCTCAGGAAAAACAAACCAAAAAAGCTGAAGGGGAACCCCAAGAAGAGAGTCCGTTGAAA; AGTAAATCACAGGAGGAACCGAAAGATACATTTGAACATGACCCATCTGAGTCTATCGAT; GAATTTAATAAGTCATCAGCCACATCCGGTGATATTTGGCCTGGCCTTTCAGCTTATGAT; AATAGTCCTAGATCACCCCATAGTCCTTCACCTATTGCTACACCACCTAGTCAGAGTTCA; TCTTGCTCTGATGCTCCCATGCTCAGTACAGTTCACTCTGCAAAAAATACTCCTTCTCAG; CATTCACATTCCATTCAGCATAGTCCTGAAAGGTCTGGGTCTGGTTCTGTTGGAAATGGA; TCTAGTCGATACAGTCCTTCTCAGAATAGTCCAATTCATCACATCCCTTCACGAAGAAGT; CCTGCAAAGACAATCGCACCACAGAATGCTCCAAGAGATGAGTCTAGGGGCCGTTCCTCG; TTTTATCCTGATGGTGGAGATCAGGAAACTGCAAAGACTGGGAAGTTCTTAAAAAGGTTC; ACAGATGAAGAGTCTAGAGTATTCCTGCTTGATAGGGGTAATACCAGGGATAAAGAGGCT; TCAAAAGAGAAAGGATCAGAGAAAGGGAGGGCAGAGGGAGAATGGGAAGATCAGGAAGCT; CTAGATTACTTCAGTGATAAAGAGTCTGGAAAACAAAAGTTTAATGATTCAGAAGGGGAT; GACACAGAGGAGACAGAGGATTATAGACAGTTCAGGAAGTCAGTCCTCGCAGATCAGGGT; AAAAGTTTTGCTACTGCATCTCACCGGAATACTGAGGAGGAAGGACTCAAGTACAAGTCC; AAAGTTTCACTGAAAGGCAATAGAGAAAGTGATGGATTTAGAGAAGAAAAAAATTATAAA; CTTAAAGAGACTGGATATGTAGTGGAAAGGCCTAGCACTACAAAAGATAAGCACAAAGAA; GAAGACAAAAATTCTGAAAGAATAACAGTAAAGAAAGAAACTCAGTCACCTGAGCAGGTA; AAGTCTGAAAAGCTCAAAGACCTCTTTGATTACAGTCCCCCTCTACACAAGAATCTGGAT; GCACGAGAAAAGTCTACCTTCAGAGAGGAAAGCCCACTTAGGATCAAAATGATAGCGAGT; GATTCTCACCGTCCTGAAGTCAAACTCAAAATGGCACCTGTTCCTCTTGATGATTCTAAC; AGACCTGCTTCCTTGACTAAAGACAGGCTGCTTGCTAGTACACTTGTCCATTCTGTCAAG; AAGGAGCAAGAATTCCGATCCATCTTTGACCACATTAAGTTGCCACAGGCCAGCAAAAGC; ACTTCAGAGTCATTTATTCAACACATTGTGTCCTTGGTTCATCATGTTAAAGAGCAATAC; TTCAAGTCAGCTGCAATGACCCTAAACGAGCGGTTCACTTCGTATCAGAAAGCCACTGAA; GAACATAGTACTCGGCAAAAGAGCCCTGAAATACACAGGAGAATTGACATCTCACCAAGT; ACCCTGAGGAAGCATACCCGTTTAGCAGGGGAAGAGAGAGTTTTTAAAGAAGAAAATCAA; AAGGGAGATAAAAAATTAAGGTGTGACTCTGCTGACCTTCGGCATGACATTGATCGCCGT; AGAAAAGAAAGAAGTAAAGAACGGGGAGATTCCAAGGGCTCCAGGGAATCCAGTGGATCA; AGAAAGCAGGAAAAAACTCCAAAAGATTACAAGGAATACAAATCTTACAAAGATGACAGT; AAACATAAAAGAGAGCAAGATCATTCTCGATCTTCATCCTCTTCAGCATCACCTTCTTCT; CCCAGTTCTCGAGAAGAAAAGGAGAGTAAGAAGGAAAGAGAAGAAGAATTTAAAACTCAC; CATGAAATGAAAGAATACTCAGGCTTTGCAGGAGTTAGCCGACCACGAGGAACCTTTTTT; CGAATTAGAGGCAGAGGAAGAGCCAGAGGAGTTTTTGCTGGGACAAATACTGGTCCAAAC; AACTCAAATACTACTTTTCAAAAGAGACCGAAGGAAGAGGAATGGGATCCAGAATATACC; CCAAAGAGCAAGAAGTACTTCTTGCATGACGACAGAGATGATGGTGTGGATTATTGGGCC; AAAAGAGGAAGAGGTCGTGGTACTTTTCAACGTGGCAGAGGGCGCTTTAACTTCAAAAAA; TCAGGTAGCAGTCCTAAATGGACTCATGACAAATACCAAGGGGATGGGATTGTTGAAGAT; GAAGAAGAGACCATGGAAAATAATGAAGAAAAGAAGGACAGACGCAAGGAAGAAAAGGAA; TAA; ; Clone variation with respect to NM_014739.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014739 unedited; NGGGGTTCAGATTTGTATCCGACTCACTATAGGCGGCCGCCGAATTCGCACGAGGGTTCT; TGAGTCGGGAAAGGCCGCTGTGTGGTTGCAACGGAGATAAATTCCCGGAACCGCGATTCG; GCGTGTCAGGAATTCGAATTTAGAGTTTAATTTCTCAGAGCATTCTCTCCAGGAAGAATT; TTTACAGTATCTCAAAGACTTCACTTGACTTCTTGATCCTGCATAAAACCAAGGAGAAAA; GAAATGGGTCGCTCCAATTCTAGATCACATTCTTCAAGGTCAAAGTCTAGATCACAGTCT; AGTTCTCGATCAAGATCAAGATCTCATTCTAGAAAGAAGCGATACAGTTCTAGGTCTCGT; TCCAGAACATATTCAAGGTCTCGTAGTAGAGATCGTATGTATTCTAGAGATTATCGTCGC; GATTACAGAAATAATAGAGGAATGAGACGACCTTATGGGTACAGAGGAAGGGGTAGAGGG; TATTATCAAGGAGGAGGAGGTAGATATCATCGAGGTGGTTATAGACCTGTCTGGAATAGA; AGGCACTCTAGGAGTCCTAGACGAGGTCGTTCACGTTCCAGGAGTCCAAAAAAGAAGATC; CGTTTCTTCTCAAAGATCCAGAAGCAGATCTCGCCGGTCATATAGATCTTCTAGGTCTCC; AAGATCATCCTCTTCTCGTTCTTCATCCCCATATAGCAAATCTCCTGTTTCTAAAAGACG; AGGGTCTCAGGAAAAACAAACCAAAAAAGCCTGAAGGGAACCCCCAAGAAGAGAGTCCGC; TGAAAAGTAAATCCCAGGAGGTAACCGAAAGATACATTTGAACATGACCCATCTGAGTCT; ATCGATGAATTTAATAAGTCATCAGCCCCATCCGGTGATATTTTGCCTCGCCTTTCAGCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014739 unedited; ACCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCAGGTA; TTACTTGTGACATTTTATTGGCGTATGACAATTTACAAGGGTCCCTGTTGCATCATTATA; CATCACACTGAGTAAGAATCGTTTAGCCATCTACATTCAATGTTACTGGGTAATATTTTT; CTCAAATTATAATTCCCAACACTGATTTTACCTGTGACAAAAGGAACAATAGTAATTCCT; TGAAGAAGACTAACTGGAAAAAACATTTTGCTTTTAGTATAAAAATTCCTAGGATGCTGA; AAGGTACACATACACACCTAAAGAGTCATGGCCTTCTTAAACAGCTTTCTTAATCCTTTC; TGGAAATATCCTTTGGTTCATTTTTATTGCCCCTCTCTAGGCAAAACAAAGTATGTTAAC; GCAGGTATCAGTGAGTTATTTCCTAGCACTTGTAAGCAAATATCCTTACCAAGAGGAACC; ATTCAACTTTTATAATATCGTAAAGCGTGGAGTTAAGATGTGTTTTTAAAAAATATACAG; GCTTTTTATATGCTTGGATCTGAAAAAGGTAACATCCACAATGACATTCTATTACAGAGT; TCTTACAATCACCCTAGCCTACTACACTCTGGTATAATACTCTTTCTTCAATTCTGTTTA; ACAGAATAAAAGTAACCAAAGGTTAAAGCCATGCATTTGAACTTAAAATATAACCTGCCC; ACAGGAATTAAGTAGTTTTATTCCCACTCCCTATTACATAATTTCTCAGATCCCCTTTTA; CCTATNNGTCTTAGTGGATAGCACATAGTCATGCCACAGATTTGATGTCTACCAAGTAAC; TGTTATGGTATTTATTGTATACAATAAAGGGTCATGAAAATTCTGTGTTGGGAATACATC; CTGTTGCTTTCCACT;
• Restriction Sites: NotI-NotI
• ACCN: NM_014739
• Insert Size: 5550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014739.1, NP_055554.1
• RefSeq Size: 5538 bp
• RefSeq ORF: 2763 bp
• Locus ID: 9774
• UniProt ID: Q9NYF8
• Cytogenetics: 6q23.3
• Protein Families: Transcription Factors
• Summary: This gene encodes a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins. The protein localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1), also known as btfL, represents the longest transcript and encodes the longest isoform (1).