ARHGAP25 (NM_014882) Human Untagged Clone

SKU SC114733

ARHGAP25 (untagged)-Human Rho GTPase activating protein 25 (ARHGAP25), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: ARHGAP25
• Synonyms: HEL-S-308; KAIA0053
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_014882 edited; ATGTCCCTCGGTCAGTCGGCCTGTCTGTTCCTCTCTATAGCTCGGTCAAGGAGTGTGATG; ACTGGCGAGCAGATGGCTGCCTTCCATCCATCGTCCACCCCCAACCCGCTGGAGAGGCCC; ATCAAGATGGGCTGGCTGAAGAAGCAGAGGTCCATCGTGAAGAACTGGCAGCAGAGGTAC; TTTGTGCTGAGGGCGCAGCAGCTCTACTACTACAAGGATGAAGAGGACACGAAGCCCCAG; GGCTGCATGTATCTACCAGGATGTACAATCAAGGAGATCGCCACAAACCCAGAAGAAGCT; GGGAAGTTTGTCTTTGAAATCATTCCAGCCTCATGGGACCAGAATCGCATGGGACAGGAC; TCCTATGTCCTCATGGCCAGCTCTCAGGCGGAGATGGAGGAGTGGGTTAAATTCCTCAGG; AGAGTTGCTGGCACACCCTGTGGAGCAGTGTTTGGCCAGCGCTTGGATGAGACTGTGGCC; TATGAACAGAAATTCGGCCCCCATCTGGTGCCCATCCTGGTGGAGAAATGTGCAGAGTTC; ATCCTGGAGCACGGCCGGAATGAAGAGGGCATCTTCCGTCTGCCTGGGCAGGACAACCTG; GTGAAGCAGCTGAGAGACGCTTTTGATGCTGGGGAGCGGCCCTCCTTTGACAGAGACACA; GATGTGCACACTGTGGCTTCCCTGTTAAAGCTCTACCTCCGAGACCTCCCAGAGCCCGTG; GTTCCCTGGAGCCAGTACGAAGGGTTCCTGCTCTGTGGGCAGCTCACGAATGCGGATGAG; GCAAAGGCTCAGCAGGAGTTGATGAAGCAGCTCTCCATCCTTCCTCGTGACAACTATAGT; CTCCTGAGCTACATCTGCAGGTTCCTACATGAAATACAGCTGAACTGTGCTGTTAACAAG; ATGAGTGTGGACAACCTGGCTACTGTGATTGGTGTGAATCTCATCAGGTCGAAGGTCGAA; GACCCTGCCGTGATCATGAGAGGGACTCCTCAGATCCAAAGAGTGATGACTATGATGATC; AGAGACCATGAAGTCCTCTTCCCCAAGTCCAAGGATATACCCCTGTCACCCCCTGCCCAG; AAAAATGACCCCAAGAAAGCTCCAGTGGCCCGAAGCTCTGTAGGCTGGGATGCCACTGAA; GACCTCCGAATTTCTAGGACAGACAGCTTCAGTAGCATGACAAGCGACTCTGATACAACC; AGCCCCACCGGACAGCAGCCGAGCGATGCGTTTCCGGAGGACAGCAGCAAAGTACCCAGG; GAAAAGCCAGGAGACTGGAAAATGCAATCTCGTAAAAGGACTCAAACACTCCCTAACCGG; AAATGTTTCTTGACATCAGCTTTTCAGGGTGCCAACAGCAGCAAAATGGAGATCTTTAAA; AATGAATTCTGGTCGCCTTCCTCAGAGGCTAAGGCAGGGGAAGGGCACAGGAGAACGATG; TCTCAAGACTTGCGCCAACTTTCTGACTCCCAACGGACTTCCACCTACGATAACGTCCCT; TCCCTGCCAGGGTCCCCTGGGGAGGAAGCCAGTGCACTCTCTTCCCAAGCCTGTGACTCC; AAGGGAGATACTCTTGCCAGTCCAAACTCTGAAACTGGGCCTGGAAAAAAGAACTCTGGA; GAAGAGGAAATTGATTCTTTGCAGAGGACGGTCCAAGAGCTACGAAAGGAAATAGAAACA; CAGAAGCAAATGTATGAGGAACAGATTAAAAACCTTGAGAAGGAAAATTATGACGTTTGG; GCTAAAGTGGTGAGGCTCAATGAAGAACTGGAGAAGGAAAAGAAGAAGTCTGCAGCCCTA; GAGATCAGCCTCCGCAACATGGAGCGCTCCCGGGAGGATGTTGAGAAGAGGAACAAGGCC; TTGGAAGAAGAAGTCAAGGAATTTGTCAAATCCATGAAGGAACCCAAGACCGAGGCTTAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014882 unedited; GTTCCGATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGGCCAACCTTT; CGGTTTGCAGAATGACGGGGCCCGCCGCGGTGCCGGACTGCCTGTGCACGGGGCCACCGA; CTGCAGCCTGGGTTTTATTCTTGGCCTGGCCCTGACCGGGAGCTGGCCCCTCGGCTGCTT; CTCTGGCTCGGGGGGGACTTTCTCTGGCTCAGATCCGGACCCCTGAACTGGACCTGGTTG; TCGTCCCCCCGCTTCTCAGCCCCCTCTGGGGTTCCTCTGTCCTCTCCGCCCACTCTTTGC; TCACTGCCCCATGTCCCTCGGTCAGTCGGCCTGTCTGTTCCTCTCTATAGCTCGGTCAAG; GAGTGTGATGACTGGCGAGCAGATGGCTGCCTTCCATCCATCGTCCACCCCCAACCCGCT; GGAGAGGCCCATCAAGATGGGCTGGCTGAAGAAGCAGAGGTCCATCGTGAAGAACTGGCA; GCAGAGGTACTTTGTGCTGAGGGCGCAGCAGCTCTACTACTACAAGGATGAAGAGGACAC; GAAGCCCCAGGGCTGCATGTATCTACCAGGATGTACAATCAAGGAGATCGCCACAAACCC; AGAAGAAGCTGGGAAGTTTGTCTTTGAAATCATTCCAGCCTCATGGGACCAGAATCGCAT; GGGACAGGACTCCTATGTCCTCATGGCCAGCTCTCNAGCGGAGATGGAGGAGTGGGTTAA; ATTCCTCAGGAGAGTTGCTGGCACACCCTGTGGAGCAGTGTTTGGCCAGCGCTTGGATGA; GACTGTGGCCTATGAACAGAAATTCGGCCCCCATCTGGTGCCCATCCTGGTGGAGAAATG; TGCAGAGTTCATCCTGNAGCACGGCCGGATGAAAAGGCATCTTTCGTCTGCCTGGGCAGN; ACAACCTGNTGAAGCAGCTGAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014882 unedited; GTACCGCGGCACGACTTCTATGTCGAGTTTTTTTTTTTTTTTTTTGCTTTATTTGAATAT; AATTTTCTCATCAATTTTGCTTGCATAAATACAGTTTGTTGCTTGTTCTGGTTTAGTCTA; ATAGTAAGTTGGAGAAGTCCTGGAGAATGGGCATCCTGGTGGGGTGGCAGAATGGGGTTC; TCGCTTCAGCAGATGCAGCCCCCCAAACACAGCACTCCATGGATGAGGACTCAAAAGATG; AATGTCATTTTCTGCCCCTGTGTCTCATAGATGCCCTGAGGCTGTCTGACAAGGTGAGCT; GTTCCACACAAAAAACAAAGAATTAATCACACCTTATTCCAGCCTGAGTTGGGGCCAGTC; TATTGCCTGGGTCCTGAGATGTGCTGCCTCAAATGTCTTCACTGGACGTGGTCATGCCTG; AGACCAGAATGGAATAGAACAATCCATTTGAATACAGCGATGGATGGTCAGAGATGTCCA; CACACATCTCTGCTCACCCTCACCTTCCTGCAAACTCCATTCATTTATTTATGGGGAAAT; TTGATGTTTCCCTCAAATGTCCTTTCCCTCTCAGAGAATAATTTTGTTTCCCCGTCATCA; GATAGCACTGAGAAAGGCGCCAGAGTTGGGCCTCTCTGGAGCTGTCCCTGCAGTACTCCT; GGGACCCTTAAGCCTCGGTCTTGGGTTCCTTCATGAATTTGACAAATTCCTTGACTTTCT; CTTTCAAGGCCTTAGTCCTCTTCTCAACATCCTNCCGGGAGCGCTCATGTTGCAGGAGCT; GATCTCTAGGGCTGGAGACTTCTTNCTTTCCTTCTTCAGTTTTATTGAGCCTCACACCTT; AGCCCAAACGTATTATTTTCCTTTCAAGGTTTTAAATCGGTCCCTAAACATTTGCCTCCG; AGTTTCTATTCCTTCCGAAGCTCTTGGACCGNCCTTGCCAAGAACCAATTCCCCTNTCCA; CAATTTTTTTTTCAGCCCCGTTTTAAAGTTGGACTGCCAAAGTACTCCTTGAAGTC;
• Restriction Sites: NotI-NotI
• ACCN: NM_014882
• Insert Size: 2890 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014882.2, NP_055697.1
• RefSeq Size: 2957 bp
• RefSeq ORF: 1917 bp
• Locus ID: 9938
• UniProt ID: P42331
• Cytogenetics: 2p13.3
• Domains: PH, RhoGAP
• Summary: ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).[supplied by OMIM, Mar 2008]; Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (b) is shorter and has a distinct N-terminus, compared to isoform a.