ARHGEF9 (NM_015185) Human Untagged Clone

SKU SC114710

ARHGEF9 (untagged)-Human Cdc42 guanine nucleotide exchange factor (GEF) 9 (ARHGEF9), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: ARHGEF9
• Synonyms: COLLYBISTIN; DEE8; EIEE8; HPEM-2; PEM-2; PEM2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_015185, the custom clone sequence may differ by one or more nucleotides; ; ; ATGACGTTGCTGATCACTGGAGATTCCATCGTTAGTGCTGAGGCAGTATGGGATCACGTCACCATGGCCA; ACCGGGAGTTGGCATTTAAAGCTGGCGACGTCATCAAAGTCTTGGATGCTTCCAACAAGGATTGGTGGTG; GGGCCAGATCGACGATGAGGAGGGATGGTTTCCTGCCAGCTTTGTGAGGCTCTGGGTGAACCAGGAGGAT; GAGGTGGAGGAGGGGCCCAGCGATGTGCAGAACGGACACCTGGACCCCAATTCAGACTGCCTCTGTCTGG; GGCGGCCACTACAGAACCGGGACCAGATGCGGGCCAATGTCATCAATGAGATAATGAGCACTGAGCGTCA; CTACATCAAGCACCTCAAGGATATTTGTGAGGGCTATCTGAAGCAGTGCCGGAAGAGAAGGGACATGTTC; AGTGACGAGCAACTGAAGGTAATCTTTGGGAACATTGAAGATATCTACAGATTTCAGATGGGCTTTGTGA; GAGACCTGGAGAAACAGTATAACAATGATGACCCCCACCTCAGCGAGATAGGACCCTGCTTCCTAGAGCA; CCAAGATGGATTCTGGATATACTCTGAGTATTGTAACAACCACCTGGATGCTTGCATGGAGCTCTCCAAA; CTGATGAAGGACAGCCGCTACCAGCACTTCTTTGAGGCCTGTCGCCTCTTGCAGCAGATGATTGACATTG; CTATCGATGGTTTCCTTTTGACTCCAGTGCAGAAGATCTGCAAGTATCCCTTACAGTTGGCTGAGCTCCT; AAAGTATACTGCCCAAGACCACAGTGACTACAGGTATGTGGCAGCTGCTTTGGCTGTCATGAGAAATGTG; ACTCAGCAGATCAACGAACGCAAGCGACGTTTAGAGAATATTGACAAGATTGCTCAGTGGCAGGCTTCTG; TCCTAGACTGGGAGGGCGAGGACATCCTAGACAGGAGCTCGGAGCTGATCTACACTGGGGAGATGGCCTG; GATCTACCAGCCCTACGGCCGCAACCAGCAGCGGGTCTTCTTCCTGTTTGACCACCAGATGGTCCTCTGC; AAGAAGGACCTAATCCGGAGAGACATCCTGTACTACAAAGGCCGCATTGACATGGATAAATATGAGGTAG; TTGACATTGAGGATGGCAGAGATGATGACTTCAATGTCAGCATGAAGAATGCCTTTAAGCTTCACAACAA; GGAGACTGAGGAGATACATCTGTTCTTTGCCAAGAAGCTGGAGGAAAAAATACGCTGGCTCAGGGCTTTC; AGAGAAGAGAGGAAAATGGTACAGGAAGATGAAAAAATTGGCTTTGAAATTTCTGAAAACCAGAAGAGGC; AGGCTGCAATGACTGTGAGAAAAGTCCCTAAGCAAAAAGGTGTCAACTCTGCCCGCTCAGTTCCTCCTTC; CTACCCACCACCGCAGGACCCGTTAAACCACGGCCAGTACCTGGTCCCCGACGGCATCGCTCAGTCGCAG; GTCTTTGAGTTCACCGAACCCAAGCGCAGCCAGTCACCATTCTGGCAAAACTTCAGCAGGTTAACCCCCT; TCAAAAAATGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015185 unedited; CATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGGGAAAACGAG; CGAAGTAAAGGGAGTAGGGGAGACTGAGACTGACCGGTAGCCAGGCAGGCGGACGGACGC; ACGCCCGGACAGACTGAGCAGGCGCCGGAGAACCACTCACAGGTTCCCCCCGCCTTTCCC; TTTGAAAGCTAGGATTTTGCCTTTCCCGTGGCGCCCGAGAGAGAATGCTGGACTCTGCCG; ACTTCAGCGCAAGCTAAGATTTCTCAGCTAGGGACAAACGATCAGCCCAATCCTGAGAAG; GGGGGAACCAAGCACCCCGTCCCCATCCCCCTCCCCTCCCCCGACTAAACTCGGGCGCCA; AACCCAGCCCTTCTCTAACCACCCTACTTCCTCCTCTCCTTTCTAGCATGGTGGCTGTAT; GGACAGTCTGACAGAACAGAGACTGACATCTCCCAATCTGCCGGCCCCCCACCTGGAACA; CTACAGTGTTCTGCATTGCACCATGACCCTGGATGTGCAAACTGTAGTCGTTTTTGCCGT; GATTGTAGTCCTCCTGCTTGTCAATGTCATACTCATGTTTTTCCTGGGAACGCGCTGAAT; GGAGTCCAGCCACCTGAGCTGTCGCGAACTCTCGCTNTGATTTCATCCCGAGAGCCACCG; AGAAAAAAAAAAAATCACAGACAGAGACAGGGAAAGAGAGAGAAAGAACAAGCTNTCTTA; CTCAGGGGGGAANAACGTTNTGAGCTTCAACATGGCCTCGCTGTGATATCTGATCACTGG; AGATTCCATCGTTAGTGCTGNAGCAGTATGGGATCACGTCACCATGGCCAACCGGNNAGT; GGGCATTAAAGCTGGCGACGTCATCAAAGTCTGGGATGCTTNCACAGGNATNGTTGGTGG; NGCCANATCGACGATAGGNAGGGATGGGTTCCTGCCAGCTTGTGAGGCTCTGGGTGACAN; NAGNATTAGTGNAGGAGGGCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015185 unedited; CGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATAATTTTATTTACTTTATT; TTAAAATTATCTGCCTCCCTGTAGGTATCATTTTTTGAAGGGGGTTAACCTGCTGAAGTT; TTGCCAGAATGGTGACTGGCTGCGCTTGGGTTCGGTGAACTCAAAGACCTGCGACTGAGC; GATGCCGTCGGGGACCAGGTACTGGCCGTGGTTTAACGGGTCCTGCGGTGGTGGGTAGGA; AGGAGGAACTGAGCGGGCAGAGTTGACACCTTTTTGCTTAGGGACTTTTCTCACAGTCAT; TGCAGCCTGCCTCTTCTGGTTTTCAGAAATTTCAAAGCCAATTTTTTCATCTTCCTGTAC; CATTTTCCTCTCTTCTCTGAAAGCCCTGAGCCAGCGTATTTTTTCCTCCAGCTTCTTGGC; AAAGAACAGATGTATCTCCTCAGTCTCCTTGTTGTGAAGCTTAAAGGCATTCTTCATGCT; GACATTGAAGTCATCATCTCTGCCATCCTCAATGTCAACTACCTCATATTTATCCATGTC; AATGCGGCCTTTGTAGTACAGGATGTCTCTCCGGATTAAGTCCTTCTTGCAGAGGACCAT; CTGGTGGTCAAACAGGAAGAATACCCGCTGCTGGTTGCGGCCGTNAGGCTGGTANATCCA; GGCCATCTCCCCAGTGTAGATCAGCTCCGAGCTCCTGTCTAGGATGTCCTCGCCCTCCCA; GTCTATGACAGAAGCCTGCCACTGAGCAATCTTGTCAATATTCTCTAAACGTCGCTTGCG; TTCGTTGATCTGCTGAGTCACATTTCTCATGACAGCCAAAGCAGCTGCCACATACCTGTT; AGTCACTGGTGGTCTTTGGGCAGTATACCTTTAGGAGCTCAGCCACCTGTAAAGGATACT; TGCAGATCTTCTGCCCTTAGTCAAAAGGAACCATCGATAGCATGTCATCATCTGCTGCAG; AAGCGACAGCCTCAATAATTGCTGGGTACCN;
• Restriction Sites: NotI-NotI
• ACCN: NM_015185
• Insert Size: 2380 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015185.1, NP_056000.1
• RefSeq Size: 5413 bp
• RefSeq ORF: 1551 bp
• Locus ID: 23229
• UniProt ID: O43307
• Cytogenetics: Xq11.1
• Domains: PH, RhoGEF, SH3
• Summary: The protein encoded by this gene is a Rho-like GTPase that switches between the active (GTP-bound) state and inactive (GDP-bound) state to regulate CDC42 and other genes. This brain-specific protein also acts as an adaptor protein for the recruitment of gephyrin and together these proteins facilitate receceptor recruitement in GABAnergic and glycinergic synapses. Defects in this gene are the cause of startle disease with epilepsy (STHEE), also known as hyperekplexia with epilepsy, as well as several other types of cognitive disability. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2017]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).