RAB21 (NM_014999) Human Untagged Clone

SKU SC114700

RAB21 (untagged)-Human RAB21, member RAS oncogene family (RAB21)

Product Data

• Type: Human Untagged Clone
• Target Symbol: RAB21
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114700 sequence for NM_014999 edited (data generated by NextGen Sequencing); ATGGCTGCGGCCGGCGGCGGCGGCGGCGGGGCGGCGGCGGCGGGCCGAGCCTACTCGTTC; AAGGTGGTGCTGCTGGGGGAAGGCTGCGTGGGGAAGACGTCGCTGGTGCTGCGCTACTGC; GAGAACAAGTTTAACGACAAGCACATCACCACTCTGCAGGCATCATTCTTAACAAAGAAG; TTAAATATTGGTGGGAAAAGAGTAAACCTTGCCATATGGGATACGGCAGGTCAAGAGAGA; TTCCATGCATTGGGTCCAATTTACTACAGAGATTCAAATGGAGCGATTTTAGTTTATGAC; ATAACAGATGAAGATTCTTTTCAGAAGGTAAAAAACTGGGTCAAAGAATTACGGAAAATG; TTGGGAAATGAAATCTGTTTATGTATAGTTGGTAATAAAATAGACTTGGAAAAGGAGAGA; CATGTTTCCATTCAAGAAGCAGAGTCGTATGCAGAATCTGTGGGAGCAAAACATTATCAT; ACTTCAGCCAAACAGAACAAAGGAATTGAGGAACTCTTTCTTGACCTTTGTAAAAGGATG; ATAGAAACAGCACAAGTGGATGAGAGAGCAAAAGGCAATGGCTCTAGTCAGCCGGGAACT; GCAAGGCGAGGTGTACAGATTATTGATGATGAACCTCAAGCCCAGACCAGTGGTGGAGGG; TGCTGTTCTTCTGGATAA; ; Clone variation with respect to NM_014999.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014999 unedited; GAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTTCCAGGCCTCGCCCG; AGGAGGGCGTGGGGACAGCGCCCGGGTCGGCGGGGCCGGGGCGGTGGGGGCTGAGCCGGC; CGTGGCTGTGAAGGCGCTGCCGCGGCTGTCGGGAGGGCGGCGCGACACTCGGGCTCGGGC; GGCCGGGAAGCGACGGGATGGCTGCGGCCGGCGGCGGCGGCGGCGGGGCGGCGGCGGCGG; GCCGAGCCTACTCGTTCAAGGTGGTGCTGCTGGGGGAAGGCTGCGTGGGGAAGACGTCGC; TGGTGCTGCGCTACTGCGAGAACAAGTTTAACGACAAGCACATCACCACTCTGCAGGCAT; CATTCTTAACAAAGAAGTTAAATATTGGTGGGAAAAGAGTAAACCTTGCCATATGGGATA; CGGCAGGTCAAGAGAGATTCCATGCATTGGGTCCAATTTACTACAGAGATTCAAATGGAG; CGATTTTAGTTTATGACATAACAGATGAAGATTCTTTTCAGAAGGTAAAAAACTGGGTCA; AAGAATTACGGAAAATGTTGGGAAATGAAATCTGTTTATGTATAGTTGGTAATAAAATAG; ACTTGGAAAAGGAGAGACATGTTTCCATTCAAGAAGCAGAGTCGTATGCAGAATCTGTGG; GAGCAAAACATTATCATACTTCAGCCAAACAGAACANAGGAANTGAGGAAACTCTTCTTG; ACCTTTGTNAAAGGATGATAGANACAGCACCAGTGGATGAGAGAGCAAAAGGCATGGCTC; TAGTCAGCCGGGAACTGCANGGCGAGGTGTACANGATATTGATGATGAACCTCAAGCCCA; GACCGTGGTGGAGGGTGCTGTTCTTCTGGATACCTGTCACGCTAAAAAATTAAGACAAGA; CAACTGTGGATCATGCCCTCACAATGAGACTGCATAATCCAGTCCATAT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014999 unedited; CGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTAAAGTTACACAAATATAATTC; TTTAATAAATCTTTCCAGTGTTCACTATATCTGCATTACATGTAATGTTTCCATCCACAG; AAACTCAGGATAGTTCTTGTAAAATGAAACAGAACATTCTGTGTTCATCTCTGGCAAGCA; ATGAACAAATAGAACTTGCTCACAAAGGATCTAAAAGTGCTGCCTCAGGAACATAAACTA; CGTGCTATATGAATGAGTCTTGGTTAACTGGCTACTATCTGGGATCTCTTCCTCTGCTCA; TACCACTGAACCCATCTCTTATAAAGCCTATGAGAAAATGAAAGCTATTGCAGAAGTGAA; ATTCAACATGAGCCACCCAAATTCTTCTAAGGTAACCATTCTAAAGCAAAATCAGCAGAA; GTCATCTCTTCTATAATAACAAACAAAAAACCCAAAAGGCAAATGCTACTGCTCAATCAT; CAGATGAACTCACAGAGGCAAAAATATGTGACAACAGCAATAATTCAAGTAAAGTATGTA; TGAAAATTTAAACACGCCATGTTCAAGTTAAGACAGAAGTGAAGGGGAGCTATGATCTTT; ATATTAGTCAAAAAAGCTAGGCATAATCACCAAATGTTAATTCACTTTACAGTCATCAAT; GAGACTATTGTATATATTTTTGCACCTTGCGCTTGTAAAAGCAGATAAAATATTTAATAT; GTAATTTACAATGTATTTTACCAGCTCAGCATACAGGATGTATACAGATTATATCAAAGT; AGTAGACAGTTACGGGCCTGTGCAAACCCTGCAAAATAGTACTTCAGATATCTGCTATTT; AAAACAAAACAGTTNNGCTGTTAGAGNTGATACATTTCACTACCCAAATACAAGGGCTGC; AAAATGTACCTTNCACAACTATATTAGTATAATAAAGATTGATTGAAGAATTAATGAGAC; TTTAGTAGTCTTTCTCAGCTATAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_014999
• Insert Size: 2440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014999.1, NP_055814.1
• RefSeq Size: 2630 bp
• RefSeq ORF: 678 bp
• Locus ID: 23011
• UniProt ID: Q9UL25
• Cytogenetics: 12q21.1
• Domains: RAB, RAN, ras, RAS, RHO
• Protein Families: Druggable Genome
• Summary: This gene belongs to the Rab family of monomeric GTPases, which are involved in the control of cellular membrane traffic. The encoded protein plays a role in the targeted trafficking of integrins via its association with integrin alpha tails. As a consequence, the encoded protein is involved in the regulation of cell adhesion and migration. Expression of this gene is associated with a poor prognosis for glioma patients. This gene is downregulated by the tumor suppressor miR-200b, and miRNA-200b is itself downregulated in glioma tissues. [provided by RefSeq, Nov 2015]