FAN1 (NM_014967) Human Untagged Clone

SKU SC114695

FAN1 (untagged)-Human FANCD2/FANCI-associated nuclease 1 (FAN1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: FAN1
• Synonyms: hFAN1; KIAA1018; KMIN; MTMR15
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114695 sequence for NM_014967 edited (data generated by NextGen Sequencing); ATGATGTCAGAAGGGAAACCTCCTGACAAAAAAAGGCCTCGTAGAAGCTTATCAATCAGC; AAGAATAAGAAAAAAGCATCTAATTCTATTATTTCGTGTTTTAACAATGCACCACCTGCT; AAACTTGCCTGCCCCGTTTGCAGTAAAATGGTGCCTAGATATGACTTAAACCGGCACCTT; GATGAAATGTGTGCTAACAATGACTTCGTTCAAGTGGATCCAGGGCAGGTTGGCTTAATA; AATTCAAATGTGTCTATGGTAGATTTAACCAGTGTTACCTTAGAAGATGTAACACCTAAG; AAGTCACCACCACCAAAGACAAATTTAACCCCTGGCCAAAGTGATTCAGCAAAAAGGGAA; GTAAAGCAGAAGATCAGTCCCTACTTTAAAAGTAATGATGTGGTGTGCAAAAATCAAGAT; GAGCTGAGAAATCGTAGTGTGAAAGTCATTTGTTTGGGAAGCCTAGCATCTAAATTGTCC; AGAAAATACGTAAAGGCTAAAAAATCAATAGATAAGGATGAAGAATTTGCCGGTTCTAGT; CCACAGAGTTCCAAATACACAGTTGTTAAGAGCCTGATTGATAACTCTTCAGAAATTGAG; GACGAGGATCAAATTTTGGAGAACAGTTCTCAAAAAGAAAACGTGTTTAAATGTGATTCT; CTAAAGGAAGAGTGCATTCCTGAACATATGGTAAGAGAAAGTAAAATAATGGAAGCCGAA; AGCCAAAAGGCTACCCGGGAATGTGAGAAATCAGCCCTCACCCCTGGATTCTCAGATAAT; GCGATCATGTTATTCTCACCAGATTTCACTCTTAGGAATACATTAAAGTCTACTTCAGAA; GACAGTCTTGTAAAGCAAGAGTGTATCAAAGAAGTGGTTGAAAAACGTGAGGCATGTCAT; TGTGAAGAAGTAAAAATGACTGTTGCTTCAGAAGCTAAAATACAGCTGTCAGATTCAGAG; GCAAAATCTCATAGTTCTGCAGATGATGCTTCTGCATGGAGTAACATCCAAGAGGCTCCT; CTGCAGGATGACAGTTGCTTAAACAATGATATCCCTCACAGCATTCCTTTGGAGCAGGGG; TCAAGCTGCAATGGTCCTGGTCAAACAACCGGTCATCCTTACTACCTTCGGAGTTTCCTT; GTGGTGCTGAAAACCGTACTTGAGAATGAAGATGATATGTTGCTCTTTGATGAGCAGGAG; AAGGGAATTGTAACTAAATTTTATCAGTTATCAGCTACTGGTCAGAAGTTATATGTAAGG; CTCTTTCAACGTAAATTAAGCTGGATTAAGATGACCAAATTAGAGTATGAAGAGATTGCC; TTAGACTTAACACCTGTGATTGAAGAATTGACGAATGCAGGCTTTCTACAGACAGAATCT; GAGTTGCAAGAACTCTCTGAAGTGCTTGAACTCCTTTCTGCTCCTGAACTAAAATCCCTA; GCCAAGACCTTCCACTTGGTGAATCCCAATGGACAGAAACAGCAGCTGGTGGACGCCTTT; CTCAAATTGGCCAAACAGCGTTCAGTCTGCACTTGGGGCAAGAATAAGCCTGGAATTGGT; GCAGTGATTTTAAAAAGAGCCAAAGCCTTGGCTGGACAGTCAGTACGAATCTGTAAAGGC; CCCAGGGCTGTGTTTTCCCGCATCTTGCTACTGTTTTCGTTGACCGACTCAATGGAAGAT; GAAGACGCCGCTTGTGGAGGTCAGGGACAGCTTTCAACAGTCCTGTTGGTCAACCTCGGC; CGAATGGAGTTTCCTAGTTACACCATCAATCGGAAAACCCACATCTTCCAAGACAGAGAT; GATCTTATCAGATATGCAGCAGCCACGCACATGCTGAGTGACATTTCTTCCGCAATGGCC; AATGGGAACTGGGAAGAAGCTAAGGAGCTCGCTCAGTGTGCAAAAAGGGATTGGAACAGA; CTGAAAAACCACCCTTCTCTGAGATGCCACGAAGATTTACCACTCTTCCTGCGGTGTTTC; ACTGTTGGGTGGATTTATACAAGGATTTTGTCTCGGTTTGTGGAAATACTGCAGAGACTT; CACATGTATGAGGAAGCCGTCAGAGAACTTGAAAGCCTTTTGTCTCAGAGAATTTATTGT; CCTGACAGCAGAGGCCGATGGTGGGATCGACTGGCCCTTAATTTACACCAGCACTTGAAG; CGCCTGGAACCGACTATCAAGTGCATCACAGAGGGGCTGGCGGATCCGGAAGTCAGAACG; GGACACCGCCTTTCACTGTATCAGCGAGCCGTGCGCCTGCGAGAGTCTCCGAGCTGTAAA; AAGTTCAAGCACCTCTTCCAGCAGCTCCCAGAAATGGCTGTGCAAGATGTGAAACACGTG; ACCATCACAGGCAGGCTGTGCCCACAGCGTGGGATGTGCAAGTCTGTGTTTGTGATGGAG; GCCGGGGAGGCCGCTGACCCCACCACGGTCCTGTGCTCTGTGGAGGAGCTGGCACTGGCC; CATTACAGACGCAGCGGTTTTGACCAGGGGATTCATGGCGAAGGGTCCACCTTCAGCACC; CTGTATGGCCTCCTCCTGTGGGACATCATCTTCATGGATGGGATTCCGGATGTCTTCAGA; AACGCCTGTCAGGCATTCCCCCTGGACTTGTGCACAGACAGCTTCTTCACAAGCAGACGC; CCAGCCCTTGAGGCCAGGCTGCAGCTGATTCATGATGCCCCCGAGGAGAGCCTGCGGGCC; TGGGTGGCAGCCACGTGGCATGAGCAGGAAGGCAGAGTGGCTTCCCTTGTCAGCTGGGAT; CGCTTCACGTCTCTTCAGCAAGCTCAGGATCTTGTCTCCTGCCTGGGGGGCCCTGTGCTC; AGTGGTGTGTGCAGGCACCTGGCTGCTGACTTTCGACACTGTCGAGGGGGCCTCCCCGAC; CTGGTGGTGTGGAACTCCCAGAGCCGTCACTTTAAGCTGGTGGAAGTTAAAGGCCCCAAT; GATCGTCTTTCACATAAGCAGATGATCTGGCTGGCTGAACTGCAGAAGCTGGGGGCTGAA; GTAGAAGTCTGCCACGTGGTTGCAGTTGGAGCTAAGAGCCAAAGCCTTAGCTAA; ; Clone variation with respect to NM_014967.4; 557 c=>a;698 g=>a;3015 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014967 unedited; NGGTCGACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCANAAATGTCGA; GACGAATAACATGAGGTCATATAGAATCCCACTTTTGGTGATTTCAAGTCAAGAAAGTAA; AAGTAAACCATTGCTATCTTTCACCTTAAATATCCTGTGTTTTATTGCTCAGAACATCCA; GTTTTTCTAATACTCATGATGTCAGAAGGGAAACCTCCTGACAAAAAAAGGCCTCGTAGA; AGCTTATCAATCAGCAAGAATAAGAAAAAAGCATCTAATTCTATTATTTCGTGTTTTAAC; AATGCACCACCTGCTAAACTTGCCTGCCCCGTTTGCAGTAAAATGGTGCCTAGATATGAC; TTAAACCGGCACCTTGATGAAATGTGTGCTAACAATGACTTCGTTCAAGTGGATCCAGGG; CAGGTTGGCTTAATAAATTCAAATGTGTCTATGGTAGATTTAACCAGTGTTACCTTAGAA; GATGTAACACCTAAGAAGTCACCACCACCAAAGACAAATTTAACCCCTGGCCAAAGTGAT; TCAGCAAAAAGGGAAGTAAAGCAGAAGATCAGTCCCTACTTTAAAAGTAATGATGTGGTG; TGCAAAAATCAAGATGAGCTGAGAAATCGTAGTGTGAAAGTCATTTGTTTGGGAAGCCTA; GCATCTAAATTGTCCAGAAAATACGTAAAGGCTAAAAAATCAATAGATAAGGATGAAGAA; TTTGCCCGGTTCTAGTCCACAGAGTTCCAAATACACAGTTGTTAAGAGCCTGATTGATAA; CTCTTCAGAAATTGAGACGANGATCAAATNNTGGAGAACAGTTCTCANAAGAAAAACGTG; TTAAATGTGATTCTCTAAAGGAGAGTGCATTNCCTGACATATGGNTAGAGAAAAGTAAAT; ATGGAAGCCGAAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014967 unedited; CCCCCTTACTCTGCACCGCGGCCCGCAATCTACACTTTTTTTTTTTTATTCTGCACAATT; TACTTATTTATTTTGCAGGAATTTGCTATAAGCAAAAACATCCAATTGGGTGATGAGAAG; GGCTTAAAATTCCCCTCTGTTTGGGACTGGTCTCTCCAGTTTACAGCAAAGGATCGCACC; CTTTTCCATAACCCCTTCTACATTGGAAAGAGCACACCTTGTCTACAGAATGGCTCCGTG; AAGTCTTTTAAACGGACAAAGGTAAATCACAGCTAACAAAACGTGATGCTGGCTCACACG; TAACCAAACACCTCTTTTTCAGAACAGAGAGCGTTAAAAGTAAAGGCACTTCCAAGAGTA; ACACTGCTAATGCGGATTCCTGAGGGGTCATTCCCTTTTTAACTCAAATGACTGTATCCC; AACTTTCTTCCTGGCGTCTGAGGCCCACAAAGTCTCAGTACCTGACAGTGGGCCCATTGC; AGCTTTGAGCCTGTCAGCCTGACCTACTACACCCTCATTTATCCATTTCTTGCTGATCCA; ACCCGACACTGCGGAAGGGAATGCCGCCTGCCTGTGACGTCACCATGGCCTCCTGACTGC; AATCTTTTTGACCTAACAACACTTCTTGTTCGCCCTTCTACCCATTCTCACCTCACTCCT; GGTCCTCTCTCCGGCCCCAACACTCGCTACATCCCTCCCCGGTACCTTCACCACCAACCC; AACATTTGGTCCTCCTCCCCTGCATTTGATACCATCCGCAACATGACCTCTTTACCATTA; ACCAGACACACGTTTCCTTCCTCACCCACACCCTACCCCCTCCCCTCTACTCACTCTACT; GGTAACCAGGACCTTCTCTCTCCCCCTCCCACAGCTCTTCAACTACCACTCCCTTATCAT; GTCTCCCCTCTCTTACCTTCCTCCTCTGCCCTCATAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_014967
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014967.3, NP_055782.2
• RefSeq Size: 4835 bp
• RefSeq ORF: 3054 bp
• Locus ID: 22909
• UniProt ID: Q9Y2M0
• Cytogenetics: 15q13.3
• Domains: ZnF_Rad18
• Summary: This gene plays a role in DNA interstrand cross-link repair and encodes a protein with 5' flap endonuclease and 5'-3' exonuclease activity. Mutations in this gene cause karyomegalic interstitial nephritis. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Feb 2016]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a).