RIPX (RUFY3) (NM_014961) Human Untagged Clone

SKU SC114693

RUFY3 (untagged)-Human RUN and FYVE domain containing 3 (RUFY3), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: RIPX
• Synonyms: RIPX; SINGAR1; ZFYVE30
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114693 sequence for NM_014961 edited (data generated by NextGen Sequencing); ATGTCTGCTCTGACGCCTCCGACCGATATGCCAACCCCCACCACTGACAAGATCACACAG; GCTGCCATGGAGACCATCTACCTTTGCAAATTCCGAGTGTCCATGGATGGAGAATGGCTC; TGCCTGCGAGAGCTGGATGACATCTCACTTACACCTGACCCAGAGCCTACCCATGAAGAT; CCTAATTATCTCATGGCTAATGAACGCATGAACCTCATGAACATGGCCAAGCTGAGTATC; AAGGGCTTGATTGAATCAGCTCTGAACCTGGGGAGGACTCTTGACTCTGACTATGCACCT; CTCCAGCAATTCTTTGTGGTGATGGAGCACTGTCTGAAACATGGCTTGAAAGCTAAAAAA; ACTTTTCTCGGACAAAATAAATCCTTCTGGGGGCCTCTAGAACTGGTAGAAAAGCTTGTT; CCAGAAGCCGCAGAGATAACAGCAAGTGTTAAAGATCTTCCAGGACTTAAGACACCAGTA; GGTAGAGGAAGAGCCTGGCTTCGTTTGGCATTAATGCAAAAGAAACTTTCAGAATATATG; AAAGCTTTGATCAATAAGAAAGAACTTCTCAGTGAATTCTACGAACCCAATGCCCTCATG; ATGGAAGAAGAAGGAGCCATAATTGCTGGTCTGTTGGTGGGTCTGAATGTCATTGATGCC; AATTTCTGTATGAAAGGAGAAGACTTGGACTCTCAGGTTGGAGTTATAGATTTTTCAATG; TATCTCAAGGACGGGAACAGCAGTAAAGGTACTGAAGGAGACGGTCAGATTACTGCAATT; CTGGACCAGAAGAACTATGTAGAAGAACTGAACAGACATTTGAATGCTACTGTAAACAAC; CTTCAGGCAAAAGTAGATGCATTAGAAAAATCCAACACTAAACTGACAGAGGAGCTTGCA; GTTGCAAACAACAGGATCATTACCTTACAAGAAGAAATGGAACGAGTTAAAGAGGAAAGT; TCCTACATACTGGAATCCAATCGGAAGGGTCCCAAGCAAGACAGAACTGCAGAAGGGCAA; GCACTAAGTGAAGCAAGAAAGCATTTAAAAGAAGAGACACAATTACGATTGGATGTTGAG; AAAGAACTGGAGATGCAGATCAGCATGAGGCAGGAGATGGAATTGGCTATGAAGATGCTG; GAGAAGGATGTCTGTGAGAAGCAGGATGCCCTGGTATCTCTTCGGCAGCAGCTGGATGAC; CTCAGAGCTCTCAAGCATGAACTTGCCTTTAAGCTGCAGAGTTCAGACTTAGGAGTAAAA; CAGAAAAGTGAACTAAACAGTCGCTTGGAAGAGAAGACTAATCAGATGGCTGCTACCATT; AAACAACTTGAACAAAGTGAAAAGGATTTGGTGAAACAGGCAAAGACCTTAAATAGTGCA; GCAAATAAACTGATCCCAAAACATCATTAG; ; Clone variation with respect to NM_014961.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014961 unedited; TACGACTCACTATAGGCGGCCCGCGATTCGGCACGAGGATTTCTGCAGCTCAGGATTTTT; TTTTTAACTACATTGAAAATATAGGTTTATTTTTTGTTCAGGTTTTTCTTTTATATTTTT; TTTCTGCACAAAGGAGGAGGATTTTTCACTTACTCATATCGAGGCCAGATTTTTAAAGCC; AGCTAAGGCAGCATCAGCTGTGCGGGATTTAAAGCCTATAGCTCAGCTGAAAAAAAAGGT; GGGGGGCAGGGAAGGGAAGATAAAAGGAGAGGAAGCTGGGAGAAGACAAGCATCATCTTA; TTTTGCTATGTGGTAGGAACTGTCTATAAGATAGTGTAGAATTGTTTATCTTGAGCAGTT; TGTTCTTAACCTATAAGGTATTTTTCCTTTTTTTTTTTTTTAAACCTCCCCCACCCTTTT; CCTGAAAGCTTTGTTTCAGAGCTTTGTATTGGGTTTTTTTGGTGAGGAGGTTGTATTTAT; TTTTTTGGTGTGTGTGTGTGAGTGTGTGTGTGTCTGTGTGTGTGTTGGGGTCCCAGCTGA; GTCATCATGTCTGCTCTGACGCCTCCGACCGATATGCCAACCCCCACCACTGACAAGATC; ACACAGGCTGCCATGGAGACCATCTACCTTTGCAAATTCCGAGTGTCCATGGATGGAGAA; TGGCTCTGCCTGCGAGAGCTGGATGACATCTCACTTACACCCTGACCAGAGCCTACCCAT; GAAGATCTATTATCTCATGGCTAATGACGCATGACCCTATGACATGGCCAGCTGATATCA; GGGCTTGATTGATCANCTCTGAACTGGGGAGACTCTGACTTGACATGCACTCTCACATTC; TTGTGGGGAGGAGCCTGTCTGAACATGCTGAAGCTAAAAACTTTCTCGACAATAATCTTC; GGGGCTCTAAACTGTAAAACTGTCCAAACCAGATACAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014961 unedited; GTACGCGGCCGCAATCTAGTGTCGAGTTTTTTTTTTTTTTTTTTGATTTTCCATTTTTTT; TATTCTAGTACTACAGTTTACAGCCATTAGATGATAAACAATAAAACATCACTTTTTAGA; AAATCCATATCCAAAGCCAACCTCAAGTACACAGTAATTTAAGTTAAATCCACTTTTTAA; AAAAATTTCCTAATAGGTTTGGTACTTAATTTTTCTGTTTTACACACATAACTGGAACTT; GTAAATGGGCAACTACCTTCTTATATGTGGAATTATAGTTTTTTCTTGTCCTTTCCCCCA; AACCACATATATTAAATCATGGGACATTATATATCAACCCTCACCTAAGCTTTGGGTTAT; TCTATAAGGAAAAGCCTTTGTCAGAGTTTGGCTGTCTCCTTCCTTTCCCATTCCCACAAA; AGATGTGTGACTAAGTGGACAAAGAACTCAGCACTGGGCCTGTTTAAGACTCTGACCATA; TTCTCCAGAAGATTCTGATGGATGTTTTCTTCTACATATCTTATAACTTTGCACCTATAG; AGAAGCATGTTTTATAAAGTAAAGCACTTGGGGCTTTATATAATAGAATTTAAACGCATG; CTATGTGTAAGTTTTAGAAAGTTCAATTTCGGTATTTGATCACTCCTTCCAAATGCTCGT; AGATAAGGCAACCCATCACCACANAAGGTTGATGAAGAGAAGAATACAGTAGGAGCTTTG; TTTATATAGGTGCAAATTATAAGATCTAAAATAGTTCAAGAGCACAGGATCTTCCCTTTC; ATGAAGTAGGTCTAAACATATGTCTTTGGACTAATTTTGGTTCAAGTCCAATCTGTGCAG; GGGACTATATAAGCACACATGGAATCTATCTTACACTCTAATTCTGGNTTTGCCAAGAAA; AAGATAAGAATCTAANAAGCTGGAACGAAGGGGTTATCGGGTCCCCACAAAGCAGCTGAA; TGCTGCNAGCCTCCCCGATGGAAAAAGAGAAAAACCCAGTTTTTGTCAGACTTTTGAAGA; AG;
• Restriction Sites: NotI-NotI
• ACCN: NM_014961
• Insert Size: 4720 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014961.2, NP_055776.1
• RefSeq Size: 4304 bp
• RefSeq ORF: 1410 bp
• Locus ID: 22902
• UniProt ID: Q7L099
• Cytogenetics: 4q13.3
• Domains: RUN
• Summary: This gene encodes a RPIP8, UNC-14, and NESCA domain-containing protein that is required for maintenance of neuronal polarity. In addition, it has been implicated in mediation of gastric cancer cell migration and invasion via interaction with P21-activated kinase-1, which promotes its expression. The encoded protein localizes to F-actin-enriched invadopodia to induce formation of protrusions, thereby facilitating cell migration. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]; Transcript Variant: This variant (2) has multiple differences in the 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1.