Argininosuccinate Lyase (ASL) (NM_000048) Human Untagged Clone

SKU SC114664

ASL (untagged)-Human argininosuccinate lyase (ASL), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: Argininosuccinate Lyase
• Synonyms: ASAL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000048, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCCTCGGAGAGTGGGAAGCTTTGGGGTGGCCGGTTTGTGGGTGCAGTGGACCCCATCATGGAGAAGT; TCAACGCGTCCATTGCCTACGACCGGCACCTTTGGGAGGTGGATGTTCAAGGCAGCAAAGCCTACAGCAG; GGGCCTGGAGAAGGCAGGGCTCCTCACCAAGGCCGAGATGGACCAGATACTCCATGGCCTAGACAAGGTG; GCTGAGGAGTGGGCCCAGGGCACCTTCAAACTGAACTCCAATGATGAGGACATCCACACAGCCAATGAGC; GCCGCCTGAAGGAGCTCATTGGTGCAACGGCAGGGAAGCTGCACACGGGACGGAGCCGGAATGACCAGGT; GGTCACAGACCTCAGGCTGTGGATGCGGCAGACCTGCTCCACGCTCTCGGGCCTCCTCTGGGAGCTCATT; AGGACCATGGTGGATCGGGCAGAGGCGGAACGTGATGTTCTCTTCCCGGGGTACACCCATTTGCAGAGGG; CCCAGCCCATCCGCTGGAGCCACTGGATTCTGAGCCACGCCGTGGCACTGACCCGAGACTCTGAGCGGCT; GCTGGAGGTGCGGAAGCGGATCAATGTCCTGCCCCTGGGGAGTGGGGCCATTGCAGGCAATCCCCTGGGT; GTGGACCGAGAGCTGCTCCGAGCAGAACTCAACTTTGGGGCCATCACTCTCAACAGCATGGATGCCACTA; GTGAGCGGGACTTTGTGGCCGAGTTCCTGTTCTGGGCTTCGCTGTGCATGACCCATCTCAGCAGGATGGC; CGAGGACCTCATCCTCTACTGCACCAAGGAATTCAGCTTCGTGCAGCTCTCAGATGCCTACAGCACGGGA; AGCAGCCTGATGCCCCAGAAGAAAAACCCCGACAGTTTGGAGCTGATCCGGAGCAAGGCTGGGCGTGTGT; TTGGGCGGTGTGCCGGGCTCCTGATGACCCTCAAGGGACTTCCCAGCACCTACAACAAAGACTTACAGGA; GGACAAGGAAGCTGTGTTTGAAGTGTCAGACACTATGAGTGCCGTGCTCCAGGTGGCCACTGGCGTCATC; TCTACGCTGCAGATTCACCAAGAGAACATGGGACAGGCTCTCAGCCCCGACATGCTGGCCACTGACCTTG; CCTATTACCTGGTCCGCAAAGGGATGCCATTCCGCCAGGCCCACGAGGCCTCCGGGAAAGCTGTGTTCAT; GGCCGAGACCAAGGGGGTCGCCCTCAACCAGCTGTCACTGCAGGAGCTGCAGACCATCAGCCCCCTGTTC; TCGGGCGACGTGATCTGCGTGTGGGACTACGGGCACAGTGTGGAGCAGTATGGTGCCCTGGGCGGCACTG; CGCGCTCCAGCGTCGACTGGCAGATCCGCCAGGTGCGGGCGCTACTGCAGGCACAGCAGGCCTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000048 unedited; GTGAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGGANAGAGAGAGA; GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA; GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGCGGAGGTGAGTGCGCGGCGG; CCGGATGGGCGGGACGGGCGTGGAGGACGCCGAGCACCGTGGCGCGCGCTCACGTCCGCG; TCCCCAAGGGCTGCGCTCCCTCAAGCGCAGTGCCCAGAACTCGGAGCCAGCCCGGCCCGG; GGGACCCTGCTGGCCAAGGAGGTCGTCAGTCCGGTCTTGTCTTCCAGACCCGGAGGACCG; AAGCTTCCGGACGACGAGGAACCGCCCAACATGGCCTCTGAGAGTGGGAAGCTTTGGGGT; GGCCGGTTTGTGGGTGCAGTGGACCCCATCATGGAGAAGTTCAACGCGTCCATTGCCTAC; GACCGGCACCTTTGGGAGGTGGATGTTCAAGGCAGCAAAGCCTACAGCTGGGGCCTGGAG; AAGGCAGGGCTCCTCACCAAGGCCGAGATGGACCAGATACTCCATGGCCTAGACAAGGTG; GCTGAGGAGTGGGCCCAGGGCACCTTCAAACTGAACTCCAATGATGAGGACATCCACACA; GCCAATGAGCGCCGCCTGAAGGAGCTCATTGGTGCAACGGCAGGGAAGCTGCTCACGGGA; CGGAGCCGNGATGACCAGGTGGTCACAGACCTCAGGCTGTGGATGCGGCAGACCTGCTTC; ACGCTCTCGGGCCTCCTCTGGGAGCTCATTATGACCATGGTGGATCGCGCAGATGCTGGA; CGTGATGTTCTCTTCCCGCGTACACCCATTTGCAGAGGGCCCAGCCCATCCGCTGGAGCC; ACTGGATTCTGAGCCACGCCGTGCACTTGACCGAGACTCTGAGCGGTTGT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000048 unedited; GCGGGCCGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTCCTCTCGCCCCCACTTTTATTAGGGGGCAGGTGTGGAAGGACCTAGGCCT; GTTGTGCCTGCAATACCCCCCGCACCTGGGGGATCTGCCATTCAACCCTGGAGCGCCCAG; TGCCCCCCAGGGCACCATACTGTTCCACACTGGGCCCGTAGTCCCACACCCAAATCACGT; CCCCCGAAAACAGGGGGCTGATGGTCTGCAACTCCTGCAGTGACAGTTGGTTGAGGGCGA; CCCCCTTGGTCTGGGCCATGAACACAGTTTTCCCGGAGGCCTTGGGGGCCTGGCGGAATG; GCATCCCTTTGCGGACCAGGTAATAGGCAAGGTCAGGGGCCACCATGTCGGGGCTGAAAC; CCTGTCCCATGTTCTCTTGGGGAATCTGCAGCGTAAAGATGACCCCAGTGGCCACCTGGA; GCACGGCACTCATAGGGTCTGACACTTAAAACACAGTTTCCTTGGCCTCCTGTAAGTCTT; TGTTGGAGGCGCTGGGAAATCCCTTGAGGGTCATCAGGAGCCCGGAACACCGCCCAAAAC; ACCCCCCACCTTGCTCGGAATAGCTCCAAACTGTTGGGGTTTTTTTTTTGGGGCATAAGG; CTGTTTCCCGTGCTGGAGCATTTGAAGCTGCCCAAACCTGAATCCTTGGGGCAATAAAGA; AAAGGCCCCCGGCCTCCTGTTGAAAGGGTATGCCCCGGAAAACCAAACAGGACCTGGCCC; AAAATCCCGTTACTATGGCATTCTGGCGTTGAAAGGATGGCCCAAATTGAATTTTGTTTG; AACAACTTTGGGCCAACCAAGGGAAGGCCGGAAGGGCCCCTTCCCCAGGGAGGAAATTTA; TCCTTTCCCACCTTAAAGCGCTAAAATTGGGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_000048
• Insert Size: 1800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000048.3, NP_000039.2
• RefSeq Size: 1937 bp
• RefSeq ORF: 1395 bp
• Locus ID: 435
• UniProt ID: P04424
• Cytogenetics: 7q11.21
• Domains: lyase_1
• Protein Pathways: Alanine, Arginine and proline metabolism, aspartate and glutamate metabolism, Metabolic pathways
• Summary: This gene encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (1).