COBRA1 (NELFB) (NM_015456) Human Untagged Clone

SKU SC114647

NELFB (untagged)-Human cofactor of BRCA1 (COBRA1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: COBRA1
• Synonyms: COBRA1; NELF-B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_015456, the custom clone sequence may differ by one or more nucleotides; ; ; ATGTTCGCGGGGCTGCAGGACCTGGGCGTGGCCAACGGCGAGGACCTGAAGGAGACCCTGACCAACTGCA; CGGAGCCGCTCAAGGCCATCGAGCAGTTCCAGACAGAGAATGGTGTGCTGCTGCCATCTCTTCAGTCAGC; CCTCCCCTTCTTGGACCTGCACGGGACGCCGCGGCTGGAGTTCCACCAGTCGGTATTCGATGAGCTGCGG; GACAAGCTGCTGGAGCGAGTGTCAGCCATCGCTTCGGAGGGGAAGGCTGAGGAAAGGTACAAGAAGCTGG; AAGACCTTCTGGAGAAGAGCTTTTCTCTGGTGAAGATGCCGTCCCTGCAGCCCGTGGTGATGTGCGTCAT; GAAGCACCTGCCCAAGGTTCCGGAGAAAAAACTGAAGCTGGTTATGGCTGACAAGGAGCTGTATCGAGCC; TGCGCCGTGGAGGTGAAGCGGCAGATCTGGCAAGACAACCAGGCCCTCTTCGGGGACGAGGTTTCCCCAC; TCCTGAAGCAGTACATCCTGGAGAAGGAGAGCGCTCTCTTCAGTACAGAGCTCTCTGTCCTGCACAACTT; TTTCAGTCCTTCCCCCAAGACCAGGCGCCAGGGCGAGGTGGTGCAGCGGCTGACGCGGATGGTGGGGAAG; AACGTGAAGCTGTACGACATGGTGCTGCAGTTTCTGCGCACGCTCTTCCTGCGCACGCGGAATGTGCACT; ACTGCACGCTGCGGGCTGAGCTGCTCATGTCCCTGCACGACCTGGACGTGGGTGAAATCTGCACCGTGGA; CCCGTGCCACAAGTTCACCTGGTGCCTGGACGCCTGCATCCGAGAGCGGTTCGTGGACAGCAAGAGGGCG; CGGGAGCTGCAGGGGTTTCTCGATGGCGTCAAGAAGGGCCAGGAGCAGGTGCTGGGGGACCTGTCCATGA; TCCTGTGTGACCCCTTCGCCATCAACACGCTGGCACTGAGCACAGTCAGGCACCTGCAGGAGCTGGTCGG; CCAGGAGACACTGCCCAGGGACAGCCCCGACCTCCTGCTGCTGCTCCGGCTGCTGGCGCTGGGCCAGGGA; GCCTGGGACATGATCGACAGCCAGGTCTTCAAGGAGCCCAAGATGGAGGTAGAGCTCATCACCAGGTTCC; TCCCGATGCTCATGTCCTTCCTGGTGGATGACTACACTTTCAATGTGGATCAGAAACTTCCGGCTGAGGA; GAAAGCCCCAGTCTCATATCCAAACACACTTCCCGAAAGCTTCACTAAGTTTCTGCAGGAGCAGCGCATG; GCCTGCGAGGTGGGGCTGTACTACGTCCTGCACATCACCAAGCAGAGGAACAAGAACGCGCTCCTCCGCC; TGCTGCCCGGGCTGGTGGAGACCTTTGGCGACTTGGCCTTTGGCGACATCTTCCTCCACCTGCTCACGGG; CAACCTTGCGCTGCTGGCCGACGAATTTGCCCTTGAGGACTTCTGCAGCAGCCTCTTCGATGGCTTCTTC; CTCACCGCCTCTCCAAGGAAGGAGAACGTGCACCGGCACGCGCTGCGGCTCCTCATTCACCTGCACCCCA; GGGTGGCCCCGTCTAAGCTGGAGGCGTTGCAGAAGGCCCTGGAGCCTACAGGCCAGAGCGGAGAGGCAGT; GAAGGAGCTTTACTCCCAGCTCGGCGAGAAGCTGGAACAGCTGGATCACCGGAAGCCCAGCCCGGCACAG; GCTGCGGAGACGCCGGCCCTGGAGCTGCCCCTCCCCAGCGTGCCCGCCCCTGCCCCGCTCTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015456 unedited; TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGAAACTTTTTCAGTCCTTCC; CCCAAAACCAGGCGCCAGGGCGAGGTGGTGCAGCGGCTGACGCGGATGGTGGGGAAGAAC; GTGAAGCTGTACGACATGGTGCTGCAGTTTCTGCGCACGCTCTTCCTGCGCACGCGGAAT; GTGCACTACTGCACGCTGCGGGCTGAGCTGCTCATGTCCCTGCACGACCTGGACGTGGGT; GAAATCTGCACCGTGGACCCGTGCCACAAGTTCACCTGGTGCCTGGACGCCTGCATCCGA; GAGCGGTTCGTGGACAGCAAGAGGGCGCGGGAGCTGCAGGGGTTTCTCGATGGCGTCAAG; AAGGGCCAGGAGCAGGTGCTGGGGGACCTGTCCATGATCCTGTGTGACCCCTTCGCCATC; AACACGCTGGCACTGAGCACAGTCAGGCACCTGCAGGAGCTGGTCGGCCAGGAGACACTG; CCCAGGGACAGCCCCGACCTCCTGCTGCTGCTCCGGCTGCTGGCGCTGGGCCAGGGAGCC; TGGGACATGATCGACAGCCAGGTCTTCAAGGAGCCCAAGATGGAGGTAGAGCTCATCACC; AGGTTCCTCCCGATGCTCATGTCCTTCCTGGTGGATGACTACACTTTCAATGTGGATCAG; AAACTTCCGGCTGAGGAGAAAGCCCCAGTCTCATATCCAAACACACTTCCCGAAAGCTTC; ACTAAGTTTCTGCAGGAGCAGCGCATGGCCTGCGAGGTGGNGCTGTACTACGTCCTGCAC; ATCACCAAGCAGAGGAACAAGAACGCGCTCCTCCGCCTGCTGCCCGGGCTGGTGGAGAAC; TTANNGCGACTGGCCTTTAGCGACATCTTCCTNNCACTGCTCACGGGCACCTTGCGCTGC; TGGCCGACGAATTGCCCTTTGAGACTCTGCAGCAGCTCTTCGAGGCTTCTTCCTACCGCT; CTNCA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015456 unedited; CCGCGGGCCGCAATCTAGAGTCGAGTTNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC; CAAATTGCCTTTTATTTACATACGAAAAAAATCTCACCGTGTGTCCAGCTGGCCCCGTCA; CGGCCGCTGAAGGCAGCACTGCTGGGCAAGGAAAAACAAGGCCACACCTGCAACCTGGGC; TGCAGGGTGAGGGGGCTCCCTCCGCACAGGCCCCAGGACACCCACCATGGCACAGGCCAG; GCTGCTGCCCCCACACGGCCTCCACGAGGTCACGGGTCCATCTCCACAACCCCAAGGCAG; TAAGCAGTGTGCCTCGCTTTCGCCAAGCTGGGCCAATGAAAAGGCCCCGCCCCCACCCTC; AGGCCCCGCCCTTTCCCCTTGGAAAACAGGTGGTTTTTTCCTAACACAGGAAAACAGAAA; ATGACCACGCTTTTTATGGCTGCCAAAGTACAAGGAGAGGCTCTGCCCTGGGAGCCACTG; CATGGATGCTGGGCAAGGTCCGGGAGCCAGCTCCAAGGAGCAGGAAGCAGGGGGCCATAC; CGGCAGGACAGGCCCACCCATAGCCCCTGCAAGAGAGACTGCCCTGTACATCCAGGCCCG; GGAGCCTCTTCCGGCTGAACAGGGCCGCGACTTCGCATGGCCCCCACACCCGACCAGCCT; CGAGGGCCCTCACTAGCGGGCAGGCGCGCACACGCTCGGGAGGGGCAACTCCAGGGCCGG; CGCCCCGCACCCGTGCCGGCTGGCCTTCCGGGACCCACTGTCCACCCTCCCCCACCGGGA; GAAAGCTTTTTCATGCCTCTCCCTCGGCCCGGAGCCCCAGGCCTTTCGCACCCTCAGTTT; AAGGGGCACCCTGGGCGCCGGAAGAGACCCCACGTGCCCCGCACTTCCCTTCTGGAAGCG; GGGGAAAAATCCTCAAAGCTTCGTAAATTCCAGGGAAATTTCGCCCCCCCAAGGTCCCGG; AGACGGGAGAAAACTCCCAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_015456
• Insert Size: 1870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015456.1, NP_056271.1
• RefSeq Size: 2622 bp
• RefSeq ORF: 2622 bp
• Locus ID: 25920
• UniProt ID: Q8WX92
• Cytogenetics: 9q34.3
• Protein Families: Transcription Factors
• Summary: NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008]