RIPOR2 (NM_015864) Human Untagged Clone

SKU SC114601

FAM65B (untagged)-Human family with sequence similarity 65, member B (FAM65B), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: RIPOR2
• Synonyms: C6orf32; DFNB104; DIFF40; DIFF48; FAM65B; MYONAP; PL48
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_015864, the custom clone sequence may differ by one or more nucleotides; ; ; ATGTTGGTAGGATCCCAGTCTTTTTCGCCTGGAGGGCCCAATGGGATCATTAGAAGCCAGTCCTTTGCGG; GTTTCAGCGGCCTCCAGGAAAGGCGATCCAGGTGTAACTCCTTCATTGAAAATTCCTCCGCTCTCAAGAA; GCCTCAGGCCAAACTGAAGAAAATGCACAATTTAGGCCACAAAAACAACAATCCCCCCAAAGAGCCTCAG; CCTAAAAGGGTGGAAGAAGTCTACAGGGCCTTGAAAAATGGACTTGATGAATATCTGGAGGTTCACCAGA; CGGAGCTGGACAAGTTGACAGCTCAGTTAAAAGATATGAAAAGAAACTCTCGCCTGGGTGTACTGTATGA; CCTAGACAAGCAAATTAAAACAATTGAAAGATACATGAGACGCCTGGAGTTTCATATAAGTAAGGTAGAT; GAACTCTATGAAGCTTATTGTATCCAGCGACGCCTCCAGGATGGTGCCAGCAAAATGAAGCAAGCCTTCG; CAACATCCCCTGCCAGCAAAGCTGCCCGGGAGAGTCTGACAGAGATCAATCGGAGCTTCAAGGAGTACAC; AGAGAATATGTGCACCATTGAAGTGGAGCTAGAGAATCTGCTGGGAGAATTCTCCATCAAGATGAAAGGT; CTGGCTGGCTTTGCACGCCTCTGTCCTGGAGATCAATATGAAATTTTCATGAAGTATGGCCGGCAGCGGT; GGAAACTGAAAGGCAAAATAGAAGTAAATGGCAAGCAGAGCTGGGATGGAGAAGAAACAGTTTTTCTGCC; CCTGATAGTTGGGTTCATCTCCATCAAGGTCACGGAGCTCAAAGGGCTAGCAACTCACATCCTGGTAGGT; AGCGTGACCTGTGAGACCAAAGAGCTGTTTGCAGCCCGACCTCAGGTAGTGGCTGTCGACATCAATGACC; TTGGTACCATCAAACTGAACCTGGAAATCACCTGGTATCCATTTGACGTGGAGGACATGACCGCATCCTC; AGGCGCTGGGAACAAGGCAGCAGCCCTTCAGAGGAGAATGTCCATGTACAGCCAGGGTACCCCGGAAACG; CCCACCTTCAAAGACCACTCCTTCTTTTCAAATCTACCTGATGACATCTTTGAAAATGGAAAGGCAGCCG; AGGAGAAAATGCCACTGTCGCTCAGCTTCAGTGACCTGCCCAACGGGGACTGCGCCCTCACCTCCCACTC; AACAGGCTCCCCTTCCAACTCAACAAATCCAGAAATTACCATCACCCCTGCGGAGTTTAACCTCAGCAGC; TTGGCCTCCCAGAATGAGGGTATGGATGACACCAGCTCAGCATCTTCCAGGAACTCCCTGGGAGAAGGCC; AAGAGCCAAAGTCACACCTGAAGGAGGAAGACCCAGAGGAGCCCAGAAAACCTGCCTCGGCCCCATCTGA; GGCTTGCCGCCGACAGTCCTCAGGTGCTGGGGCTGAGCACCTGTTCCTTGAGAATGATGTTGCAGAAGCA; CTTCTGCAAGAGTCTGAGGAGGCCTCTGAGCTCAAGCCTGTGGAACTGGACACTTCGGAAGGAAACATCA; CAAAGCAGCTGGTCAAGAGGCTCACATCTGCAGAGGTGCCAATGGCCACAGACAGGCTGCTCTCTGAGGG; TTCTGTTGGTGGAGAATCTGAAGGCTGCAGATCCTTTCTAGATGGAAGCTTAGAGGATGCTTTTAATGGG; CTTTTACTTGCATTAGAACCACATAAAGAGCAGTATAAAGAGTTTCAGGATCTGAACCAAGAAGTCATGA; ATTTGGATGATATTCTAAAAAAGTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015864 unedited; TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCTGCCTGCTGCTTCT; CGCGCCTGTCCTCTCCAANATGCAGTTTTTTGATGCTGAGGAGCTCCTGGTCGATGAAGA; GGATGATGTTTTTGGTGAAGGACTACCGACCAGACTCCCGGAAATCATGTTGGTAGGATC; CCAGTCTTTTTCGCCTGGAGGGCCCAATGGGATCATTAGAAGCCAGTCCTTTGCGGGTTT; CAGCGGCCTCCAGGAAAGGCGATCCAGGTGTAACTCCTTCATTGAAAATTCCTCCGCTCT; CAAGAAGCCTCAGGCCAAACTGAAGAAAATGCACAATTTAGGCCACAAAAACAACAATCC; CCCCAAAGAGCCTCAGCCTAAAAGGGTGGAAGAAGTCTACAGGGCCTTGAAAAATGGACT; TGATGAATATCTGGAGGTTCACCAGACGGAGCTGGACAAGTTGACAGCTCAGTTAAAAGA; TATGAAAAGAAACTCTCGCCTGNGTGTACTGTATGACCTAGACAAGCAAATTAAAACAAT; TGAAAGATACATGAGACGCCTGGAGTTTCATATAAGTAAGGTAGATGAACTCTATGAAGC; TTATTGTATCCAGCGACGCCTNCAGGATGGTGCCAGCANAATGAAGCAAGCCTTCGCACA; TCCNCTGCCAGCAAGCTGCCCGGGAGAGTCTGACAGAGATCAATCGGAGCTTCAAGGAGT; ACACAGAGAATATGTGCACCATTGAAGTGGAGCTAGAGAATCTGCTGGGAGAATTCTCAT; CAGATGAAAGGTCTGGCTGCTTTGCACGCTCTGTCTGGAGATCATATGAATTTCATGAAG; TATGGCCGCAGNCGTGGAACTGAAAGCAAATAAAGTAATGGCAGCAAGCTGGATGGAAAA; AACANTTTCTGCCCGAAAATGGNTCATTNCTCAGGTACGACTCAGGCTACACTCATCTGN; TAGGACGGACTGTAACAAAACTTTGACCACCTCGTAGGCTGNCATAGA;
• Restriction Sites: NotI-NotI
• ACCN: NM_015864
• Insert Size: 2560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015864.2, NP_056948.2
• RefSeq Size: 2452 bp
• RefSeq ORF: 1776 bp
• Locus ID: 9750
• UniProt ID: Q9Y4F9
• Cytogenetics: 6p22.3
• Summary: This gene encodes an atypical inhibitor of the small G protein RhoA. Inhibition of RhoA activity by the encoded protein mediates myoblast fusion and polarization of T cells and neutrophils. The encoded protein is a component of hair cell stereocilia that is essential for hearing. A splice site mutation in this gene results in hearing loss in human patients. [provided by RefSeq, Sep 2016]; Transcript Variant: This variant (2) has an alternate 5' UTR exon, it lacks an in-frame exon in the central coding region, and it lacks multiple 3' exons but includes an alternate 3' exon, compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1.