PIGT (NM_015937) Human Untagged Clone

SKU SC114534

PIGT (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class T (PIGT), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: PIGT
• Synonyms: CGI-06; MCAHS3; NDAP; PNH2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_015937, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCGGCGGCTATGCCGCTTGCTCTGCTCGTCCTGTTGCTCCTGGGGCCCGGCGGCTGGTGCCTTGCAG; AACCCCCACGCGACAGCCTGCGGGAGGAACTTGTCATCACCCCGCTGCCTTCCGGGGACGTAGCCGCCAC; ATTCCAGTTCCGCACGCGCTGGGATTCGGAGCTTCAGCGGGAAGGAGTGTCCCATTACAGGCTCTTTCCC; AAAGCCCTGGGGCAGCTGATCTCCAAGTATTCTCTACGGGAGCTGCACCTGTCATTCACACAAGGCTTTT; GGAGGACCCGATACTGGGGGCCACCCTTCCTGCAGGCCCCATCAGGTGCAGAGCTGTGGGTCTGGTTCCA; AGACACTGTCACTGATGTGGATAAATCTTGGAAGGAGCTCAGTAATGTCCTCTCAGGGATCTTCTGCGCC; TCTCTCAACTTCATCGACTCCACCAACACAGTCACTCCCACTGCCTCCTTCAAACCCCTGGGTCTGGCCA; ATGACACTGACCACTACTTTCTGCGCTATGCTGTGCTGCCGCGGGAGGTGGTCTGCACCGAAAACCTCAC; CCCCTGGAAGAAGCTCTTGCCCTGTAGTTCCAAGGCAGGCCTCTCTGTGCTGCTGAAGGCAGATCGCTTG; TTCCACACCAGCTACCACTCCCAGGCAGTGCATATCCGCCCTGTTTGCAGAAATGCACGCTGTACTAGCA; TCTCCTGGGAGCTGAGGCAGACCCTGTCAGTTGTATTTGATGCCTTCATCACGGGGCAGGGAAAGAAAGA; CTGGTCCCTCTTCCGGATGTTCTCCCGAACCCTCACGGAGCCCTGCCCCCTGGCTTCAGAGAGCCGAGTC; TATGTGGACATCACCACCTACAACCAGGACAACGAGACATTAGAGGTGCACCCACCCCCGACCACTACAT; ATCAGGACGTCATCCTAGGCACTCGGAAGACCTATGCCATCTATGACTTGCTTGACACCGCCATGATCAA; CAACTCTCGAAACCTCAACATCCAGCTCAAGTGGAAGAGACCCCCAGAGAATGAGGCCCCCCCAGTGCCC; TTCCTGCATGCCCAGCGGTACGTGAGTGGCTATGGGCTGCAGAAGGGGGAGCTGAGCACACTGCTGTACA; ACACCCACCCATACCGGGCCTTCCCGGTGCTGCTGCTGGACACCGTACCCTGGTATCTGCGGCTGTATGT; GCACACCCTCACCATCACCTCCAAGGGCAAGGAGAACAAACCAAGTTACATCCACTACCAGCCTGCCCAG; GACCGGCTGCAACCCCACCTCCTGGAGATGCTGATTCAGCTGCCGGCCAACTCAGTCACCAAGGTTTCCA; TCCAGTTTGAGCGGGCGCTGCTGAAGTGGACCGAGTACACGCCAGATCCTAACCATGGCTTCTATGTCAG; CCCATCTGTCCTCAGCGCCCTTGTGCCCAGCATGGTAGCAGCCAAGCCAGTGGACTGGGAAGAGAGTCCC; CTCTTCAACAGCCTGTTCCCAGTCTCTGATGGCTCTAACTACTTTGTGCGGCTCTACACGGAGCCGCTGC; TGGTGAACCTGCCGACACCGGACTTCAGCATGCCCTACAACGTGATCTGCCTCACGTGCACTGTGGTGGC; CGTGTGCTATGGCTCCTTCTACAATCTCCTCACCCGAACCTTCCACATCGAGGAGCCCCGCACAGGTGGC; CTGGCCAAGCGGCTGGCCAACCTTATCCGGCGCGCCCGAGGTGTCCCCCCACTCTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015937 unedited; CAGCATTTTGTAATACGACTCACTATAGGGGCGGCCGCGAATTCGCACGAGGGGCTATGC; CGCTTGCTCTGCTCGTCCTGTTGCTCCTGGGGCCCGGCGGCTGGTGCCTTGCAGAACCCC; CACGCGACAGCCTGCGGGAGGAACTTGTCATCACCCCGCTGCCTTCCGGGGACGTAGCCG; CCACATTCCAGTTCCGCACGCGCTGGGATTCGGAGCTTCAGCGGGAAGGAGTGTCCCATT; ACAGGCTCTTTCCCAAAGCCCTGGGGCAGCTGATCTCCAAGTATTCTCTACGGGAGCTGC; ACCTGTCATTCACACAAGGCTTTTGGAGGACCCGATACTGGGGGCCACCCTTCCTGCAGG; CCCCATCAGGTGCAGAGCTGTGGGTCTGGTTCCAAGACACTGTCACTGATGTGGATAAAT; CTTGGAAGGAGCTCAGTAATGTCCTCTCAGGGATCTTCTGCGCCTCTCTCAACTTCATCG; ACTCCACCAACACAGTCACTCCCACTGCCTCCTTCAAACCCCTGGGTCTGGCCAATGACA; CTGACCACTACTTTCTGCGCTATGCTGTGCTGCCGCGGGAAGGTGGTCTGCACCGAAAAC; CTCACCCCCTGGAAGAAGCTCTTGCCCTGTAGTTCCAAGGCAGGCCTCTCTGTGCTGCTG; AGGGCAGATCGCTTGTTCCACACCAGCTACCACTCCNANGCAGTGCATATNCGNNCCCTG; TTGCAGAAAATGCACGCTGTACTAGCATCTNCTGGGAGCTGANGCAGACCCTGTCAGTNG; TATTTGATGNCTTCATCACGGGGCAGGGAAAGAANNGACTGGTCCTCTNNNCCGNATGTT; CTCCCGACCCTNACGGAGCCNTGCCCCCTGGCTTANAGAANCGAGTCTATGGTGCANTNC; CNCCTACACCAGGACACGAACATTTAAAGGTGCCCCCACCCGACACTACTTANAGGGAGT; NATCTAGACTTCGGAGACATAGCATTATGACTGGTTGACCGCCTGTCAACACTTGAACTA; ATCACTAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015937 unedited; TCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTCACGGAAACAGCCGTTTTTATTCCACAAA; CAGTAAACTCCATCCACTACACAGTGACATACACTGTGTAGCACACCCCATCACCTTGGC; CAGTGCTGCCAGCCGACCTTTCTGTGGTGATGGAAATCTTTTTCTGTGCTGTCCAATACA; GCAGCCACCGACCACTTTTGCTTATTGAGCACCTCAATATAGAGGTGGCCACTACAGGTG; AGGAAATGAATTTCTAAGTTAATTCAAATTCAAATGCCACATTTGGCTCGTGGCTCCTGT; ACTGGACAACACAGCTGTAGGCCCTGACCTGGTCCAGGGCACTTTGGTTCAAAAGCCAAC; TCTGAGGAGAGCAAGTGGCAGAAACAGCCCTTGGGCTCCCCTCCCCAGAGAGAAACGGCA; GCTGCAGCTGCTGGAAAGGGCAAGAATCAGAGTGGGGGGACACCTCGGGCGCGCCGGATA; AGGTTGGCCAGCCGCTTGGCCAGGCCACCTGTGCGGGGCTCCTCGATGTGGAAGGTTCGG; GTGAGGAGATTGTAAAAGGAGCCGTANCACACGGCCACCACAGTGCACGTGAGGCANATC; ACGTTGTAGGGCATGCTGAGTCCCGTGTCNGCAGGTCACCACCAGCGGCTCCGTGTAGAG; CCGCACAAAGTATTAGAGCCATCANAGACTGGGAACAAGCTGTTGAAGAGGGGACTCTCT; TCCCAGCCACTGCCTTGGCTGCTACATGCTGGCCACAAGGCCCTGAAGACAAAGGGCTGA; CCTAAACCCATGTTAGGATCTGGGGGTACTTGGCACTTAACAAGCCCCGTCAACTGGAAG; GAACCTCGGGACCGATTGGCCGCAATTTATCACACTTCCGAAGGGGGGTGCACCCGGCCT; GCACGCCGGAAAGGAGTACCGGCTGCTCCCTGCCCTGGAGGAGGAGGGGGGCCAACGCCA; CAACCAGGCCGGGCCAACAAACCGGAAGCCGGCTGGGGTG;
• Restriction Sites: NotI-NotI
• ACCN: NM_015937
• Insert Size: 2260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015937.1, NP_057021.1
• RefSeq Size: 2246 bp
• RefSeq ORF: 1725 bp
• Locus ID: 51604
• UniProt ID: Q969N2
• Cytogenetics: 20q13.12
• Domains: Gpi16
• Protein Families: Transmembrane
• Protein Pathways: Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
• Summary: This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is an essential component of the multisubunit enzyme, GPI transamidase. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).