UBC6e (UBE2J1) (NM_016021) Human Untagged Clone

SKU SC114499

UBE2J1 (untagged)-Human ubiquitin-conjugating enzyme E2, J1, U (UBE2J1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: UBC6e
• Synonyms: CGI-76; HSPC153; HSPC205; HSU93243; NCUBE-1; NCUBE1; UBC6; UBC6E; Ubc6p
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114499 sequence for NM_016021 edited (data generated by NextGen Sequencing); ATGGAGACCCGCTACAACCTGAAGAGTCCGGCTGTTAAACGTTTAATGAAAGAAGCGGCA; GAATTGAAAGATCCAACAGATCATTACCATGCGCAGCCTTTAGAGGATAACCTTTTTGAA; TGGCACTTCACGGTTAGAGGGCCCCCAGACTCCGATTTTGATGGAGGAGTTTATCACGGG; CGGATAGTACTGCCACCAGAGTATCCCATGAAACCACCAAGCATTATTCTCCTAACGGCT; AATGGTCGATTTGAAGTGGGCAAGAAAATCTGTTTGAGCATCTCAGGCCATCATCCTGAA; ACTTGGCAGCCTTCGTGGAGTATAAGGACAGCATTATTAGCCATCATTGGGTTTATGCCA; ACAAAAGGAGAGGGAGCCATAGGTTCTCTAGATTACACTCCTGAGGAAAGAAGAGCACTT; GCCAAAAAATCACAAGATTTCTGTTGTGAAGGATGTGGCTCTGCCATGAAGGATGTCCTG; TTGCCTTTAAAATCTGGAAGCGATTCAAGCCAAGCTGACCAAGAAGCCAAAGAACTGGCT; AGGCAAATAAGCTTTAAGGCAGAAGTCAATTCATCTGGAAAGACTATCTCTGAGTCAGAC; TTAAACCACTCTTTTTCACTAACTGATTTACAAGATGATATACCTACAACATTCCAGGGT; GCTACGGCCAGTACATCGTACGGAGTCCAGAATTCCTCAGCAGCATCCTTTCATCAACCT; ACCCAACCTGTAGCTAAGAATACCTCCATGAGCCCTCGACAGCGCCGGGCCCAGCAGCAG; AGTCAGAGAAGGTTGTCTACTTCACCAGATGTAATCCAGGGCCACCAGCCAAGAGACAAC; CACACTGATCATGGTGGGTCAGCTGTACTGATTGTCATCCTGACTTTGGCATTGGCAGCT; CTTATATTCCGACGAATATATCTGGCAAACGAATACATATTTGACTTTGAGTTATAA; ; Clone variation with respect to NM_016021.2; 685 c=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016021 unedited; GTTTTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCCCGAGCCGCGCTG; AGACCCGGGCAGCGGCCGCGTGGAGAGGAGGTGGCAGCGGCCCGGGAGGCCGGAGCCAAG; CCAGCGACCCACCATGGAGACCCGCTACAACCTGAAGAGTCCGGCTGTTAAACGTTTAAT; GAAAGAAGCGGCAGAATTGAAAGATCCAACAGATCATTACCATGCGCAGCCTTTAGAGGA; TAACCTTTTTGAATGGCACTTCACGGTTAGAGGGCCCCCAGACTCCGATTTTGATGGAGG; AGTTTATCACGGGCGGATAGTACTGCCACCAGAGTATCCCATGAAACCACCAAGCATTAT; TCTCCTAACGGCTAATGGTCGATTTGAAGTGGGCAAGAAAATCTGTTTGAGCATCTCAGG; CCATCATCCTGAAACTTGGCAGCCTTCGTGGAGTATAAGGACAGCATTATTAGCCATCAT; TGGGTTTATGCCAACAAAAGGAGAGGGAGCCATAGGTTCTCTAGATTACACTCCTGAGGA; AAGAAGAGCACTTGCCAAAAAATCACAAGATTTCTGTTGTGAAGGATGTGGCTCTGCCAT; GAAGGATGTCCTGTTGCCTTTAAAATCTGGAAGCGATTCAAGCCAAGCTGACCAAGAAGC; CAAAGAACTGGCTAGGCAAATAAGCTTTAAGGCAGAAGTCAATTCATCTGGAAAGACTAT; CTCTGAGTCAGACTTAAACCACTCTTTTTCACTAACTGATTTACAAGATGATATACCTAC; AACATTCCAGGGTGCTACGGCCAGTACATCGTACGGAGTCCAGAATTCCTCAGCAGCATC; CTTTCATCAACCTACCCAACCTGTAGCTAAGAATACCTNNCATGAGCCTCGACAGCGCCG; GGCCCAGCAGCAGAGTCAGAGAGGTTGTCTACTTCCCAGATGTATCCAGGGNCACAGCCA; GAGACACACCTGATCATGTGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016021 unedited; GACGCGGCCGCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTAAAAGACTGAATTTATT; AAAGTTTAAGACAATTCTGTATTATATAATAGTGTACAATATGTTCTTATTTTAGATGTA; GAAATAAAGGAAAACTGGCTCTCAGATGAAAACAGAAATTGTGTCTGACAGTTAATAGAA; CGAATGTCCCATAAACAGGAGTAAAAATTAAATTTGCCTTTAAGACGATTTTACAATTAT; TTCTCTTGTCCATAATTTCACTTAGTTAAAACCACATAAGGACTTTATGAATACCCTCCC; CTATACTATTCAAGATATACATATGGGCTAACTTTCGTGGACCGATCTATAACCTAAATA; CCACTTCCAACATTGTTTGACTTGTAAAAAAATTAACTTACAAATAACACTTTTGGAGCA; TAACCTATTTGAAAGCTTGGAACCACCTGTATTTAAAAATTAAATCTTCATTAACAACAC; CCTCAAGAGTGCCCTCAGTGCTGCGAACTGAACATGCTGAAGGCAATGTTTTTCTGTGAT; TATATGGTTTCACCAGCCATTATGATGATAATATTATCTACTAAAGTCTGAGGCGCTAGC; TAACACTAAGTTATATATTGATTTTTCCACTGAAAATTCTTTCTTGAGCTCTGACTAAGA; GGACAATTCTAAGTAAAGGGTTTTCTAACTTCTATACCTGTTACTGGGGCCCTGAGGTTT; CCCATTAAAATAGGACCTGAATATTTCCATTTTATGCATTTTCCCGGAATAATAAAATTC; TTCCGGTTTATAACGCTGGCCTTTATACCTCCCAAAGACATCCTTTCAAGAAAACCAACA; TCCAATTCTCCAATCCCCCCTCTACTTCTGGAAATACCCCGCATTCTCTGGTTGGGCCCT; TCTTCTTAAAACACTAGAAAACCGCCTCCTTCTCTTAACTTGCGGCATATTGCAAACAAA; GGAGGGCTGGGAAACCAGTNTTAAATGCTACAAGCACACCTTCAACGAATACAGCGCCAA; TTTATCTCTNT;
• Restriction Sites: NotI-NotI
• ACCN: NM_016021
• Insert Size: 2710 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016021.2, NP_057105.2
• RefSeq Size: 4360 bp
• RefSeq ORF: 957 bp
• Locus ID: 51465
• UniProt ID: Q9Y385
• Cytogenetics: 6q15
• Domains: UBCc
• Protein Families: Transmembrane
• Protein Pathways: Parkinson's disease, Ubiquitin mediated proteolysis
• Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system. [provided by RefSeq, Jul 2008]