Suppressor of Fused (SUFU) (NM_016169) Human Untagged Clone

SKU SC114403

SUFU (untagged)-Human suppressor of fused homolog (Drosophila) (SUFU), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Suppressor of Fused
• Synonyms: JBTS32; PRO1280; SUFUH; SUFUXL
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114403 sequence for NM_016169 edited (data generated by NextGen Sequencing); ATGGCGGAGCTGCGGCCTAGCGGCGCCCCCGGCCCCACCGCGCCCCCGGCCCCTGGCCCG; ACTGCCCCCCCGGCCTTCGCTTCGCTCTTTCCCCCGGGACTGCACGCCATCTACGGAGAG; TGCCGCCGCCTTTACCCTGACCAGCCGAACCCGCTCCAGGTTACCGCTATCGTCAAGTAC; TGGTTGGGTGGCCCAGACCCCTTGGACTATGTTAGCATGTACAGGAATGTGGGGAGCCCT; TCTGCTAACATCCCCGAGCACTGGCACTACATCAGCTTCGGCCTGAGTGATCTCTATGGT; GACAACAGAGTCCATGAGTTTACAGGAACAGATGGACCTAGTGGTTTTGGCTTTGAGTTG; ACCTTTCGTCTGAAGAGAGAAACTGGGGAGTCTGCCCCACCAACATGGCCCGCAGAGTTA; ATGCAGGGCTTGGCACGATACGTGTTCCAGTCAGAGAACACCTTCTGCAGTGGGGACCAT; GTGTCCTGGCACAGCCCTTTGGATAACAGTGAGTCAAGAATTCAGCACATGCTGCTGACA; GAGGACCCACAGATGCAGCCCGTGCAGACACCCTTTGGGGTAGTTACCTTCCTCCAGATC; GTTGGTGTCTGCACTGAAGAGCTACACTCAGCCCAGCAGTGGAACGGGCAGGGCATCCTG; GAGCTGCTGCGGACAGTGCCTATTGCTGGCGGCCCCTGGCTGATAACTGACATGCGGAGG; GGAGAGACCATATTTGAGATCGATCCACACCTGCAAGAGAGAGTTGACAAAGGCATCGAG; ACAGATGGCTCCAACCTGAGTGGTGTCAGTGCCAAGTGTGCCTGGGATGACCTGAGCCGG; CCCCCCGAGGATGACGAGGACAGCCGGAGCATCTGCATCGGCACACAGCCCCGGCGACTC; TCTGGCAAAGACACAGAGCAGATCCGGGAGACCCTGAGGAGAGGACTCGAGATCAACAGC; AAACCTGTCCTTCCACCAATCAACCCTCAGCGGCAGAATGGCCTCGCCCACGACCGGGCC; CCGAGCCGCAAAGACAGCCTGGAAAGTGACAGCTCCACGGCCATCATTCCCCATGAGCTG; ATTCGCACGCGGCAGCTTGAGAGCGTACATCTGAAATTCAACCAGGAGTCCGGAGCCCTC; ATTCCTCTCTGCCTAAGGGGCAGGCTCCTGCATGGACGGCACTTTACATATAAAAGTATC; ACAGGTGACATGGCCATCACGTTTGTCTCCACGGGAGTGGAAGGCGCCTTTGCCACTGAG; GAGCATCCTTACGCGGCTCATGGACCCTGGTTACAAATCCTGTTGACCGAAGAGTTTGTA; GAGAAAATGTTGGAGGATTTAGAAGATTTGACTTCTCCAGAGGAATTCAAACTTCCCAAA; GAGTACAGCTGGCCTGAAAAGAAGCTGAAGGTCTCCATCCTGCCTGACGTGGTGTTCGAC; AGTCCGCTACACTAG; ; Clone variation with respect to NM_016169.3; 1299 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016169 unedited; NGGTGTCAAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCGGCGA; CAGCCTGGGCGGACAGTGCGCCGTGCGCAGGCGCGGAGCTAGACCTCGCTGCAGCCCCCA; TCGCCTCGGGGAGTCTCACCCACCGAGTCCGCCCGCTGGCCCGTCAGTGCTCTCCCCGTC; GTTTGCCCTCTCCAGTTCCCCCAGTGCCTGCCCTACGCACCCCGATGGCGGAGCTGCGGC; CTAGCGGCGCCCCCGGCCCCACCGCGCCCCCGGCCCCTGGCCCGACTGCCCCCCCGGCCT; TCGCTTCGCTCTTTCCCCCGGGACTGCACGCCATCTACGGAGAGTGCCGCCGCCTTTACC; CTGACCAGCCGAACCCGCTCCAGGTTACCGCTATCGTCAAGTACTGGTTGGGTGGCCCAG; ACCCCTTGGACTATGTTAGCATGTACAGGAATGTGGGGAGCCCTTCTGCTAACATCCCCG; AGCACTGGCACTACATCAGCTTCGGCCTGAGTGATCTCTATGGTGACAACAGAGTCCATG; AGTTTACAGGAACAGATGGACCTAGTGGTTTTGGCTTTGAGTTGACCTTTCGTCTGAAGA; GAGAAACTGNGGAGTCTGCCCCACCAACATGGCCCGCAGAGTTAATGCAGGGCTTGGCAC; GATACGTGTTCCAGTCAGAGAACACCTTCTGCAGTGGGGACCATGTGTCCTGGCACAGCC; CTTTGGNATACAGTGAGTCAAGAATTCAGCACATGCTGCTGACAGAGGACCCACAGATGC; AGCCCGTGCAGACACCCTTTGGGGTAGTTACCTTCCTCCAGATCGTTGGTGTCTGCACTG; AAGAGCTACACTCAGCCCAGCAGTGGAACGGGCAGGNCATCCTGGAGCTGCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016169 unedited; TGTACCGCGGCACGCAATCTAGTGTCGAGTTTTTTTTTTTTTTTTTTTCTGCAAAGAGTT; TAATGCACTTATATTACAGAGAGGAAGAGGGGGCAAGAAGGAAACGACATCTAAGTGAGT; CACCCAAAAATTCTTACAAAAGTCCCAGGCTGGCTGCAGCCCCAGGAACCGAGTCGCCAC; TGACATTAAAACTAATATAAAATTTTACAAAAATCTGCTTGTCGCAGCATTCAGGTACTT; GTACAAAAGGAGGGGTCTCCCTACAGGGCTCCTGACTTGTAACAGGGACCCAGGCACCTC; ACCTGGCATGGATGTCCTATAAGCCAGAAAGGGAAGACCAGACACAAAAATACAGTAAAG; GAAATTCTCTACTGGTCAGCCCCATAGGGGCACCGACCTTCAGGTTTTCTCCCTGCTTGC; AACTCAATCCGAAACCCCAGTCCCAAGACCCTGTGTCTATTTTTAAAAATGCTCTAGCCC; TTTTAGACTCTCCCCTTGGCTTCTCCAGTCCCTGCCTTGGAGCACTGCCCTTGGAAGAGA; AAGATACGGGCAGGAAGAGGCACCAGCTCCCCACACAGGCCTGAGACCTCGCACAAGCCT; CCCAAACCCCCCTCAGAATTGCAGCTGAGTCTGCAGTCCTCCTGCAGCCCACACTGAGCC; AGCAGAGCCACAGGATGCCTGCCTTATATAGCAGGCAAAGAGGATAGGGGAAAGGCCCTT; CTCATAAGCTCTAGCACCAGGGACCTGGAGGGGTGGGTACGACTAGGCAAGTCTNCAAGT; AGCTTGCAAAATAGCAGATTAGGNAAGGGAGGCCCNTTACTCCTNCACCACTACAAGTCA; AGGGGCTCCTCANAGCTGCAGGNAGGGAATGAAAGGTACATTCACNANCATCCTGTGCAN; AANCTGTGCTGAAGGNTNCAAGGCANCTGGTCTGATCTCTCTTAGGGGCCCTTTACANCC; ACAGCCAAGAACTTGGGAGGCCATGCCATGAAAGAGGCG;
• Restriction Sites: NotI-NotI
• ACCN: NM_016169
• Insert Size: 4640 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016169.2, NP_057253.2
• RefSeq Size: 4898 bp
• RefSeq ORF: 1455 bp
• Locus ID: 51684
• UniProt ID: Q9UMX1
• Cytogenetics: 10q24.32
• Domains: SUFU
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer
• Summary: The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]; Transcript Variant: This variant (1) encodes the longer isoform (1).