CYB5R2 (NM_016229) Human Untagged Clone

SKU SC114350

CYB5R2 (untagged)-Human cytochrome b5 reductase 2 (CYB5R2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: CYB5R2
• Synonyms: B5R.2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_016229 edited; GGCGGCCGCGAATTCGGCACGAGGCAGCATCCCCCTCCGCCCGGTCACCCAGACGGCCTT; CTCCAGCCTTGCCGAGCTTAAGACCCGTCCCTGCTCCTGACCATCACCGTCACTGGGGTC; ACTGTGCTCGTGTTGGTCCTGAAGAGCATGAACTCCAGGAGGAGAGAGCCAATCACCTTA; CAGGACCCTGAAGCCAAGTACCCGCTGCCCTTGATTGAGAAAGAGAAAATCAGCCACAAC; ACCCGGAGGTTCCGCTTTGGACTGCCTTCGCCGGACCATGTCTTAGGGCTTCCTGTAGGT; AACTATGTCCAGCTCTTGGCAAAAATCGATAATGAATTGGTGGTCAGGGCTTACACCCCT; GTCTCCAGTGATGATGACAGAGGCTTTGTGGACCTAATTATAAAGATCTACTTCAAAAAT; GTACACCCCCAATATCCTGAAGGTGGGAAGATGACTCAGTATTTGGAGAACATGAAAATC; GGGGAGACCATCTTTTTTCGAGGGCCAAGGGGACGCTTGTTTTACCATGGGCCAGGGAAT; CTTGGAATCAGACCAGACCAGACGAGTGAGCCTAAAAAAACACTGGCCGATCACCTGGGA; ATGATTGCTGGGGGCACAGGCATCACACCCATGTTGCAGCTCATTCGCCACATCACCAAG; GACCCCAGTGACAGGACCAGGATGTCCCTCATCTTTGCCAACCAGGTCAGTTCCTGCTGA; GCTTGCAGTGGTAGGATGGAATGTTTGCTGAGCTGGGCTCTTGTTTTCATTTCTCCTGCA; TTGACTGAGCTCATGCTGCTTGGGTGCCTGCATCTGTGGGAGGGCTTAGTGGGATCAACA; GGGCTGCTGGCAGCTGGAGAAGCTGAGTCCAGGCCTGCTTTTTGCATGTATTTAACTCTT; TTGTGTCTCCTCAGCCAGCCCTAAGCACTAAGCATTCCTTTTTTCCTTAAGATGTACTGC; TGTATTTTTGCTAAGTGTAAAATCACAGATGTCCTCAGTGGACACTTCAGAAAATACAGA; ATATAAATATTTACTTACTTTTTAAATCTTTTTTCTATATATGTTATATATTTG;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016229 unedited; AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAGCATCCCCCTCCGCCCG; GTCACCCAGACGGCCTTCTCCAGCCTTGCCGAGCTTAAGACCCGTCCCTGCTCCTGACCA; TCACCGTCACTGGGGTCACTGTGCTCGTGTTGGTCCTGAAGAGCATGAACTCCAGGAGGA; GAGAGCCAATCACCTTACAGGACCCTGAAGCCAAGTACCCGCTGCCCTTGATTGAGAAAG; AGAAAATCAGCCACAACACCCGGAGGTTCCGCTTTGGACTGCCTTCGCCGGACCATGTCT; TAGGGCTTCCTGTAGGTAACTATGTCCAGCTCTTGGCAAAAATCGATAATGAATTGGTGG; TCAGGGCTTACACCCCTGTCTCCAGTGATGATGACAGAGGCTTTGTGGACCTAATTATAA; AGATCTACTTCAAAAATGTACACCCCCAATATCCTGAAGGTGGGAAGATGACTCAGTATT; TGGAGAACATGAAAATCGGGGAGACCATCTTTTTTCGAGGGCCAAGGGGACGCTTGTTTT; ACCATGGGCCAGGGAATCTTGGAATCAGACCAGACCAGACGAGTGAGCCTAAAAAAACAC; TGGCCGATCACCTGNGAATGATTGCTGGGGGCACAGGCATCACACCCATGTTGCAGCTCA; TTCGCCACATCACCAAGGGACCCCAGTGACAGGACCAGGATGTCCCTCATCTTTGCCAAC; CAGGTCAGTTCCTGCTGAGCTTGCAGTGGTANGATGGAATGTTTGCTGAGCTGGGCTCTT; GNTNTCATTTCTNCTGCATTGACTGAGCTCATGCTGCNTGGNGTGCCTGCATCTGTGGGA; GGGGCTANNTGNGATCAACAGGNCTGCTGGCAGCTGGAGAAACTGAGTCCAGCCCTGCTT; TTGCATGTATTAACTCTTTTTGNGTCTCTCAGCCACCCTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016229 unedited; GGCACGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTCGCTTTGTACTTGAGTTTATT; TCACAAAACCACGGGAAAAAGATACTGGAAATGGGAGCTCTTTCCAGCTCTCCAAGCAAG; GGAGGCCCCANGCAGCCAGTCTCCANGCCCCTGTGAGCCCTTTTTTGTTAGGNCCCACCA; CCCAAAAGAGGGAGAACCAGTGTTGTGGCGCCAAGGTACATGNGCAAGGCACTTTTTGAA; AACATCCCAGTTTACCGTGGTGAAATTGAACTTACTCTGAAACAGATGAAAAGGGACATG; CAAAATTGCTGAGCACATGGAGGTGTTAGTAGGTGAAAATCATGTCCTGGGTATAACCCA; GCTTCTCCAGGTTAGGGTGAGCCGCCGTCTGGATCAGTGGTGGCGGGCCACACACCAGGA; TGAGCGTGGACTTCGCTGGAGGAGGAAGGTGCTCCTTGATCATGTCGGCAGTAACGAAGC; CTGAGCTGTACTTCCAGCCAATGGGAGGCCTGTCCAGGGTGTACCACAGGTCGAACTGGT; CTGGGTGAGTCCTGGCAATTTCTTCAAGCTCTTTTCTGACCAAGATATCCTCCTCTGTCT; AGAAAAGAAGCAACACTGAAAAAGCCCCTCCAGGGCCATCCTCTTGTTCCCTGGACTGAC; TACACCGGTCAGCATACTCCTGGCCAAAGCTGCCACCACTCCAGCTGGAGTGCTCGCTGC; CAACTCCCACGGCAAACAAGAGTTCTGCTGCTGCTCCTGAAGCTCAAACCCTTTGTTTTG; TGGATGAANGTCCTNCATGTGGATGAAAGAACTCCATGGGATGTANGNTCCNATGGAGCT; GCAGTGACCGNCTNCACTANAAGGACTTANCAAGCGCTGCCAGAATGCTCACTNNGACTC; AGCCCTGCTCTAANTACCTGAGCTTACACAGCAACTACTGGACAGCTCATGCAGCCCAGA; TCTCTGAGATGTATGCTCANA;
• Restriction Sites: NotI-NotI
• ACCN: NM_016229
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016229.2, NP_057313.2
• RefSeq Size: 1341 bp
• RefSeq ORF: 831 bp
• Locus ID: 51700
• UniProt ID: Q6BCY4
• Cytogenetics: 11p15.4
• Domains: FAD_binding_6, NAD_binding_1
• Protein Families: Druggable Genome
• Summary: The protein encoded by this gene belongs to the flavoprotein pyridine nucleotide cytochrome reductase family of proteins. Cytochrome b-type NAD(P)H oxidoreductases are implicated in many processes including cholesterol biosynthesis, fatty acid desaturation and elongation, and respiratory burst in neutrophils and macrophages. Cytochrome b5 reductases have soluble and membrane-bound forms that are the product of alternative splicing. In animal cells, the membrane-bound form binds to the endoplasmic reticulum, where it is a member of a fatty acid desaturation complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]; Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.