Aminoadipate aminotransferase (AADAT) (NM_016228) Human Untagged Clone

SKU SC114349

AADAT (untagged)-Human aminoadipate aminotransferase (AADAT), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Aminoadipate aminotransferase
• Synonyms: KAT2; KATII; KYAT2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114349 sequence for NM_016228 edited (data generated by NextGen Sequencing); ATGAATTACGCACGGTTCATCACGGCAGCGAGCGCAGCCAGAAACCCTTCTCCCATCCGG; ACCATGACTGACATATTGAGCAGAGGACCAAAATCGATGATCTCCTTGGCTGGTGGCTTA; CCAAATCCAAACATGTTTCCTTTTAAGACTGCCGTAATCACTGTAGAAAATGGAAAGACC; ATCCAATTTGGAGAAGAGATGATGAAGAGAGCACTTCAGTATTCTCCGAGTGCTGGAATT; CCAGAGCTTTTGTCCTGGCTAAAACAGTTACAAATAAAATTGCATAATCCTCCTACCATC; CATTACCCACCCAGTCAAGGACAAATGGATCTATGTGTCACATCTGGCAGCCAACAAGGT; CTTTGTAAGGTGTTTGAAATGATCATTAATCCTGGAGATAATGTCCTCCTAGATGAACCT; GCTTATTCAGGAACTCTTCAAAGTCTGCACCCACTGGGCTGCAACATTATTAATGTTGCC; AGTGATGAAAGTGGGATTGTTCCAGATTCCCTAAGAGACATACTTTCCAGATGGAAACCA; GAAGATGCAAAGAATCCCCAGAAAAACACCCCCAAATTTCTTTATACTGTTCCAAATGGC; AACAACCCTACTGGAAACTCATTAACCAGTGAACGCAAAAAGGAAATCTATGAGCTTGCA; AGAAAATATGATTTCCTCATAATAGAAGATGATCCTTACTATTTTCTCCAGTTTAACAAG; TTCAGGGTACCAACATTTCTTTCCATGGATGTTGATGGACGTGTCATCAGAGCTGACTCT; TTTTCAAAAATCATTTCCTCTGGGTTGAGAATAGGATTTTTAACTGGTCCAAAACCCTTA; ATAGAGAGAGTTATTTTACACATACAAGTTTCAACATTGCACCCCAGCACTTTTAACCAG; CTCATGATATCACAGCTTCTACACGAATGGGGAGAAGAAGGTTTCATGGCTCATGTAGAC; AGGGTTATTGATTTCTATAGTAACCAGAAGGATGCAATACTGGCAGCTGCAGACAAGTGG; TTAACTGGTTTGGCAGAATGGCATGTTCCTGCTGCTGGAATGTTTTTATGGATTAAAGTT; AAAGGCATTAATGATGTAAAAGAACTGATTGAAGAAAAGGCCGTTAAGATGGGGGTATTA; ATGCTCCCTGGAAATGCTTTCTACGTCGATAGCTCAGCTCCTAGCCCTTACTTGAGAGCA; TCCTTCTCTTCAGCTTCTCCAGAACAGATGGATGTGGCCTTCCAGGTATTAGCACAACTT; ATAAAAGAATCTTTATGA; ; Clone variation with respect to NM_016228.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016228 unedited; ACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCCGCAGGAGCGCGTGCGGCGTGGACT; TTGCCGGGCTCGCCACACAGCCCCAGACCCGTTTAGGACCGGGAGACCGAACGCAGCGTC; CAGCCGGGGAGTTTCGGCGGCGTTCTCCGGGCACCGCGCGCGGGAAGCCAGACGCAGCGG; GGGGACACATCTCGCGGTGGCGTTGCCAGAGTGAGGAGTTAGCAGGCAGGACTTGACGAG; GCTCTTTGGTTTTTCTAGTCCTCAACCACTGAAGAAGAAGCTTGATGCTTGGCTGTCAGA; AGACATGAATTACGCACGGTTCATCACGGCAGCGAGCGCAGCCAGAAACCCTTCTCCCAT; CCGGACCATGACTGACATATTGAGCAGAGGACCAAAATCGATGATCTCCTTGGCTGGTGG; CTTACCAAATCCAAACATGTTTCCTTTTAAGACTGCCGTAATCACTGTAGAAAATGGAAA; GACCATCCAATTTGGAGAAGAGATGATGAAGAGAGCACTTCAGTATTCTCCGAGTGCTGG; AATTCCAGAGCTTTTGTCCTGGCTAANACAGTTACAAATAAAATTGCATAATCCTTCTAC; CATCCATTACCCACCCAGTCAAGGACAAATGGATCTATGTGTCACATCTGGCAGCCAACA; GGTCTTTGTAAAGTGTTTGAATGATCATTATCCTGGAGATATGTCCTCCTAGATGACCTG; CTTATCANNGACTCTTCAAGTCTGCCCCACTGGGCTGCACATATTTATGTTGCCGTGATG; AAGTGGGATTGTCCAGATCCCTAGAGACATACTTTCAGATGGAACCCAGAGATGCAAGAA; TCCAGAAAACACCCCAATTTCCTATACTGTTCAATGGGCACACCTCCTGAAACTCATACA; GTGACGCAAAGGATCTTTGACTGCAGAAATGATTCTCTATANAGAGATCTACTATTCTCG; NTACAGTGAGGTCCN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016228 unedited; GGGGGGGGGGCAGGNAAAAACGAATAAAAACAGAGGNGGTGGGGGTTCAGCATCCTATTT; AAGGCATTTCTGTGGCATAACATAAGGTTTATGGTACTTTTACTAAAAGTCACTTATAAT; GACCAAATTATAACAATTTTTGCAATAAGCTCTCATTAAATTTTCCTAAAAGTAGAAAAA; GTACACATTATATACCATTTTGCACTTAATTACTTCTTTAAAATCTCAAAATAATTCAGT; GTAAAATGTTAGTTTCAAAGACAATTTATGGGAAATTACAAAGCAACTACAAAGTTCTTC; ATAATAAATCCAAGATAAAAGTAACTTTTTACAGGTTGAAAGTTTTTCAAAATGCCTAAG; TGGATTATAAATACAGTTTGGCCATCTGATGAATTTAGAGGCACTTTAAAAGGTGTCTAG; TTGAACTACTACAGATAGGACATGTTTGAGGAAATTTAATCTTTAAGTCTAATACCAGTG; TTCATTTCTTCCTGCCAAAACAAGAAATTTATTGGAAAAATCCATGAGCAGCATGATTAG; TTTGATTTCTTTCTCTTTTTTTAGAGACAGGGTCTTGTTCTGCCATGCAAGCCAGAGTGC; ATGCAGGCCAGAGAGCAGTGGTGCGATCGTAGCTTACTGCAGCCTTGAATTCCTGGGATT; AAGTGATCCTACCTCCTGTGACTTACAAAGTGCTGGGACTATACGTGGGAGCCCCCATGC; CCAACCTACTTTAATTTCTTCATAAAGAATTCTTTTATAGGTGGGCTAATACCTGGAAGG; CGCCAATCCCTCTGTTTCTGGAAAGTCTGAACAACAAGGATGCTCTTCAAGTTAGGAGCT; ACGAGCTGATCTATTCCCCTGATAAAGCCTTCCCAGGGCCCCTCTCTACCCCCCTCCTAC; GGGCTCTTTTCTTAATCAACGATCTTCATCCTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_016228
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016228.3, NP_057312.1
• RefSeq Size: 2326 bp
• RefSeq ORF: 1278 bp
• Locus ID: 51166
• UniProt ID: Q8N5Z0
• Cytogenetics: 4q33
• Protein Pathways: Lysine biosynthesis, Lysine degradation, Metabolic pathways, Tryptophan metabolism
• Summary: This gene encodes a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]; Transcript Variant: This variant (1) differs in the 5' UTR and uses an alternate in-frame splice junction at the 3' end of the first coding exon compared to variant 3. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Variants 1, 2, and 4 all encode the same isoform (b).