LCMT1 (NM_016309) Human Untagged Clone

SKU SC114308

LCMT1 (untagged)-Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: LCMT1
• Synonyms: CGI-68; LCMT; PPMT1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114308 sequence for NM_016309 edited (data generated by NextGen Sequencing); ATGGCCACTAGGCAGAGGGAATCCTCTATCACCTCCTGCTGTTCCACCTCGAGCTGCGAC; GCAGACGACGAGGGCGTGCGCGGCACCTGCGAAGATGCTTCCCTGTGCAAGAGGTTTGCA; GTAAGCATTGGCTACTGGCATGACCCTTACATACAGCACTTTGTGAGACTGTCTAAAGAG; AGGAAAGCCCCTGAAATCAACAGAGGATATTTTGCTCGAGTCCATGGTGTCAGTCAGCTT; ATAAAGGCATTTCTACGGAAGACAGAATGTCATTGTCAAATTGTCAACCTTGGGGCAGGC; ATGGATACCACCTTCTGGAGATTAAAGGATGAAGATCTTCTCCCAAGTAAATATTTTGAG; GTTGACTTTCCAATGATTGTCACGAGAAAGCTGCACAGTATCAAATGCAAGCCTCCCCTA; TCCAGCCCCATTCTAGAACTGCATTCAGAGGACACACTTCAGATGGATGGACACATACTG; GATTCAAAGAGATATGCCGTTATTGGAGCAGATCTCCGAGACCTGTCTGAACTGGAAGAG; AAGCTAAAGAAATGTAACATGAATACACAATTGCCAACACTCCTGATAGCTGAATGTGTG; CTGGTTTACATGACTCCAGAGCAGTCCGCAAACCTCCTGAAGTGGGCAGCCAACAGTTTT; GAGAGAGCCATGTTCATAAACTACGAACAGGTGAACATGGGTGATCGGTTTGGGCAGATC; ATGATTGAAAACCTGCGGAGACGCCAGTGTGACCTGGCGGGAGTGGAGACCTGCAAGTCA; TTAGAGTCACAGAAAGAACGGCTCCTGTCGAATGGGTGGGAAACAGCATCGGCCGTCGAC; ATGATGGAGTTGTACAACAGGTTACCTCGAGCTGAAGTGAGCAGGATAGAATCACTTGAA; TTCCTGGATGAAATGGAGCTGCTGGAGCAGCTCATGCGGCATTACTGCCTTTGCTGGGCA; ACCAAAGGAGGAAATGAGCTTGGGCTGAAGGAGATAACTTATTAA; ; Clone variation with respect to NM_016309.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016309 unedited; GGTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCTGAGCCAG; GTAGGGCCCTACCCTCTTCTGTTGCTTTCTCCCTGTGGCTCGCGCCGTCCCCCGCCGCCC; GTCGACCCCGCTTCCATGTCCCTGGCGGACACAGCTCCCAGGAACCTCCACGCCCATGGC; CACTAGGCAGAGGGAATCCTCTATCACCTCCTGCTGTTCCACCTCGAGCTGCGACGCAGA; CGACGAGGGCGTGCGCGGCACCTGCGAAGATGCTTCCCTGTGCAAGAGGTTTGCAGTAAG; CATTGGCTACTGGCATGACCCTTACATACAGCACTTTGTGAGACTGTCTAAAGAGAGGAA; AGCCCCTGAAATCAACAGAGGATATTTTGCTCGAGTCCATGGTGTCAGTCAGCTTATAAA; GGCATTTCTACGGAAGACAGAATGTCATTGTCAAATTGTCAACCTTGGGGCAGGCATGGA; TACCACCTTCTGGAGATTAAAGGATGAAGATCTTCTCCCAAGTAAATATTTTGAGGTTGA; CTTTCCAATGATTGTCACGAGAAAGCTGCACAGTATCAAATGCAAGCCTCCCCTATCCAG; CCCCATTCTAGAACTGCATTCAGAGGACACACTTCAGATGGATGGACACATACTGGATTC; AAAGAGATATGCCGTTATTGGAGCAGATCTCCGAGACCTGTCTGAACTGGAAGAGAAGCT; AAAGAAATGTAACATGAATACACAATTGCCAACACTCCTGATAGCTGAATGGTGTGCTGG; TTTACATGACTCCAGAGCAGTCCGCANACCTNNCTGAGTGGGCAGCCAACANGTTTGAGA; AGACCATGTTCATAAACTACGAACAGGTGAACAGGGGTGGATCGGNTTGGNGCAGATCAT; GAATNGAAACCCTGCGAGACGCCAGTGTGACCTGGCGGNNAGTGGAGACCTGCAGTCATT; AA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016309 unedited; GACCCCGCCCGAACAACCCGNGCCGCNTCCCCCTTTNNAATTTTTGACTTTGGNCCGCGG; NCCGCATACNANGATCGNGTTTTTTTTTTTTTTTTTTGGTGGCAAGTGGAAATTATTTAA; ACAACGACATGTCAACAGGGAACAGGAAGAGGAACATGTGCGACCACTGTAGTGACCAAG; GCTTCTCAAAAGTGTGGGATGAGACCTGCGGACGAGGCCCGCCCACCGCTCAGGGAGCTT; GCAGGTCTCCTCCAGGAGGGCAAGTGGCTTCGGCTTCTGGCTCGGCATAAGCCTTCGACA; GATTAATAAGTTATCTCCTTCAGCCCAAGCTCATTTCCTCCTTTGGTTGCCCAGCAAAGG; CAGTAATGCCGCATGAGCTGCTCCAGCAGCTCCATTTCATCCAGGAATTCAAGTGATTCT; ATCCTGCTCACTTCAGCTCGAGGTAACCTGTTGTACAACTCCATCATGTCGACGGCCGAT; GCTGTTTCCCACCCATTCGACAGGAGCCGTTCTTTCTGTGACTCTAATGACTTGCAGGTC; TCCACTCCCGCCAGGTCACACTGGCGTCTCCGCAGGTTTTCAATCATGATCTGCCCAAAC; CGATCACCCATGTTCACCTGTTCGTAGTTTATGAACATGGCTCTCTCAAAACTGTTGGCT; GCCCACTTCAGGAGGTTTGCGGACTGCTCTGGAGTCATGTAAACCAGCACACATTCAGCT; ATCAGGAGTGTTGGCAATTGGGTATTCATGTTACATTTCTTTAGCTTCTCTTCCAGTTCA; GACAGGTCTCGGAGATCTGCTCCAATAACGCCATATCCTCTTTGAATCCAGTATGTGTGC; ATCCATTTGAAGTGTGTCCTCTGGAAGCAAGTTTTAAAATGGGGCTGGTAAGGGGAGGCT; TGCCTTTGAACTGGCCAGCTTTTTCGTGAAAAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_016309
• Insert Size: 1380 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016309.2, NP_057393.2
• RefSeq Size: 1374 bp
• RefSeq ORF: 1005 bp
• Locus ID: 51451
• UniProt ID: Q9UIC8
• Cytogenetics: 16p12.1
• Domains: LCM
• Protein Pathways: Androgen and estrogen metabolism, Histidine metabolism, Selenoamino acid metabolism, Tyrosine metabolism
• Summary: LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A (PPP2CA; MIM 176915) (De Baere et al., 1999 [PubMed 10600115]).[supplied by OMIM, Mar 2008]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).