WWOX (NM_016373) Human Untagged Clone

SKU SC114305

WWOX (untagged)-Human WW domain containing oxidoreductase (WWOX), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: WWOX
• Synonyms: D16S432E; DEE28; EIEE28; FOR; FRA16D; HHCMA56; PRO0128; SCAR12; SDR41C1; WOX1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_016373 edited; ATGGCAGCGCTGCGCTACGCGGGGCTGGACGACACGGACAGTGAGGACGAGCTGCCTCCG; GGCTGGGAGGAGAGAACCACCAAGGACGGCTGGGTTTACTACGCCAATCACACCGAGGAG; AAGACTCAGTGGGAACATCCAAAAACTGGAAAAAGAAAACGAGTGGCAGGAGATTTGCCA; TACGGATGGGAACAAGAAACTGATGAGAACGGACAAGTGTTTTTTGTTGACCATATAAAT; AAAAGAACCACCTACTTGGACCCAAGACTGGCGTTTACTGTGGATGATAATCCGACCAAG; CCAACCACCCGGCAAAGATACGACGGCAGCACCACTGCCATGGAAATTCTCCAGGGCCGG; GATTTCACTGGCAAAGTGGTTGTGGTCACTGGAGCTAATTCAGGAATAGGGTTCGAAACC; GCCAAGTCTTTTGCCCTCCATGGTGCACATGTGATCTTGGCCTGCAGGAACATGGCAAGG; GCGAGTGAAGCAGTGTCACGCATTTTAGAAGAATGGCATAAAGCCAAGGTAGAAGCAATG; ACCCTGGACCTCGCTCTGCTCCGTAGCGTGCAGCATTTTGCTGAAGCATTCAAGGCCAAG; AATGTGCCTCTTCATGTGCTTGTGTGCAACGCAGCAACTTTTGCTCTACCCTGGAGTCTC; ACCAAAGATGGCCTGGAGACCACCTTTCAAGTGAATCATCTGGGGCACTTCTACCTTGTC; CAGCTCCTCCAGGATGTTTTGTGCCGCTCAGCTCCTGCCCGTGTCATTGTGGTCTCCTCA; GAGTCCCATCGATTTACAGATATTAACGACTCCTTGGGAAAACTGGACTTCAGTCGCCTC; TCTCCAACAAAAAACGACTATTGGGCGATGCTGGCTTATAACAGGTCCAAGCTCTGCAAC; ATCCTCTTCTCCAACGAGCTGCACCGTCGCCTCTCCCCACGCGGGGTCACGTCGAACGCA; GTGCATCCTGGAAATATGATGTACTCCAACATTCATCGCAGCTGGTGGGTGTACACACTG; CTGTTTACCTTGGCGAGGCCTTTCACCAAGTCCATGCAACAGGGAGCTGCCACCACCGTG; TACTGTGCTGCTGTCCCAGAACTGGAGGGTCTGGGAGGGATGTACTTCAACAACTGCTGC; CGCTGCATGCCCTCACCAGAAGCTCAGAGCGAAGAGACGGCCCGGACCCTGTGGGCGCTC; AGCGAGAGGCTGATCCAAGAACGGCTTGGCAGCCAGTCCGGCTAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016373 unedited; TCACATTTGTATCCGACTCACTATAGGGNNCGGCCGCGCAATTCGGCACGAGGGTCGGGC; CCCGACGCGCGCGGGTCTCGTTTGGAGCGGGAGTGAGTTCCTGAGCGAGTGGACCCGGCA; GCGGGCGATAGGGGGGCCAGGTGCCTCCACAGTCAGCCATGGCAGCGCTGCGCTACGCGG; GGCTGGACGACACGGACAGTGAGGACGAGCTGCCTCCGGGCTGGGAGGAGAGAACCACCA; AGGACGGCTGGGTTTACTACGCCAATCACACCGAGGAGAAGACTCAGTGGGAACATCCAA; AAACTGGAAAAAGAAAACGAGTGGCAGGAGATTTGCCATACGGATGGGAACAAGAAACTG; ATGAGAACGGACAAGTGTTTTTTGTTGACCATATAAATAAAAGAACCACCTACTTGGACC; CAAGACTGGCGTTTACTGTGGATGATAATCCGACCAAGCCAACCACCCGGCAAAGATACG; ACGGCAGCACCACTGCCATGGAAATTCTCCAGGGCCGGGATTTCACTGGCAAAGTGGTTG; TGGTCACTGGAGCTAATTCAGGAATAGGGTTCGAAACCGCCAAGTCTTTTGCCCTCCATG; TTGCACATGTGATCTTTGCCTGCAGGAACATGGCAAGGGCGAGTGAAGCAGTGCCACGCA; TTTTAGAAGAATGGCATAAAGCCAAGGTAGAAGCAATGACCCTGGACCTCGCTCTGCTCC; GTAACGTGCAGCATTTTTGCTGAAGCATTCAAAGCCCNGAATGTGCCTCCTTATGTGCTT; GTGTGCAACGCACCAACTTTTGCTCTACCCTGGAGTCTCACCCAAGATGGCCTGGAGACC; ACCTTTCAGATGAATCATTTGGGGGCACTTTTACCTTGTCCAGCTCCTTCAAGAAGTNTT; GGGGCCCTTAACTTCTGCCCGGGTCATTGTGGTCTCCTCAGAACN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016373 unedited; CGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGATATTCAAGCATGTTCTT; TTATTAAGCGTAGGATGCGAGGCACAGCAGGAGGTTTAAGTACAATGTGAAAGCAAGAGA; ACTGAGACTGTGATTGACAGACAAAGGGATTAACTAACGTTTTATTCTCTGCCCCCCAAA; ATATCCTGTGTATTCTTAAGTATATACGCTTCCCTTCCTGCCTTTCAAGGTATCTAAGGA; ATGATTTGAAAAATTTGTTATAATCTCTAAAGAATTTTTTGTATAGCATTAGCAAAGGAG; TCTATGACAAGTACTTTGCCACCTGGTAGTTCTGCGTATTCTACTCCCTCTGGGTGTCAC; TGTCATCCTCACTGGCTGGGACAAGGTTCTGAGATTTGTCTCCCCAGCAGTTGCTAAGCT; GGCTCAGTCTTGGTCAGGATGAATGAAACAATTATCTCCTGGATCAATGCAGCAAGGAGC; AGTGAACACTTGCTTTTTCTTCCCTAAGTAGGAGAAGGCCAGCCCCTGGCTGCAGTGGTG; GCCGTGGTGGTAGCTGGATGGGACGAGGGGACCTCAGGCTATTCTATAACAGTAAAAGAA; AGAGAATTGCTGGTGCTTCTCCAGGGAGATACGGAACCTACACGCCAACCAGGTTTTTAC; TTTCAAACAGGCCACCACCAGAAAGCAAAGAGACTATGCCTAGCCCAGCCCAGAAAGTGA; TACTNNTACCCCAGAATTCCCCTGCTTCCCATTGTACTTAAGATTNTTCACTCTGNTGGG; AATGCTNCTATTNCCTTTNACTCTGCNGATCTGTGTGGNAGGNACATTNGNAAGGGGGCC; ANCCNTGNCACTNGCGTGAGGGGACCACACAGGNNNCGGTGGGTGTGGCCCANCCCGCTC; TGAGCTCACTTTACCGACTGGCTGNNCAGCCGTNCTTGGATCANCCCTCCCTGGACGCCC; CAGGNT;
• Restriction Sites: NotI-NotI
• ACCN: NM_016373
• Insert Size: 2380 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016373.1, NP_057457.1
• RefSeq Size: 2264 bp
• RefSeq ORF: 1245 bp
• Locus ID: 51741
• UniProt ID: Q9NZC7
• Cytogenetics: 16q23.1-q23.2
• Domains: adh_short, WW
• Protein Families: Druggable Genome
• Summary: This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) protein family. This gene spans the FRA16D common chromosomal fragile site and appears to function as a tumor suppressor gene. Expression of the encoded protein is able to induce apoptosis, while defects in this gene are associated with multiple types of cancer. Disruption of this gene is also associated with autosomal recessive spinocerebellar ataxia 12. Disruption of a similar gene in mouse results in impaired steroidogenesis, additionally suggesting a metabolic function for the protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).