COX16 (NM_016468) Human Untagged Clone

SKU SC114236

COX16 (untagged)-Human COX16 cytochrome c oxidase assembly homolog (S. cerevisiae) (COX16), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: COX16
• Synonyms: C14orf112; hCOX16; HSPC203
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114236 sequence for NM_016468 edited (data generated by NextGen Sequencing); ATGTTTGCACCCGCGGTGATGCGTGCTTTTCGCAAGAACAAGACTCTCGGCTATGGAGTC; CCCATGTTGTTGCTGATTGTTGGAGGTTCTTTTGGTCTTCGTGAGTTTTCTCAAATCCGA; TATGATGCTGTGAAGAGTAAAATGGATCCTGAGCTTGAAAAAAAACTGAAAGAGAATAAA; ATATCTTTAGAGTCGGAATATGAGAAAATCAAAGACTCCAAGTTTGATGACTGGAAGAAT; ATTCGAGGACCCAGGCCTTGGGAAGATCCTGACCTCCTCCAAGGAAGAAATCCAGAAAGC; CTTAAGACTAAGACAACTTGA; ; Clone variation with respect to NM_016468.6;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016468 unedited; TAGAATTTGTATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGCTGAGATTTGG; GAGTCTGCGCTAGGCCCGCTTGGAGTTCTGAGCCGATGGAAGAGTTCACTTCATGTTTGC; ACCCGCGGTGATGCGTGCTTTTCGCAAGAACAAGACTCTCGGCTATGGAGTCCCCATGTT; GTTGCTGATTGTTGGAGGTTCTTTTGGTCTTCGTGAGTTTTCTCAAATCCGATATGATGC; TGTGAAGAGTAAAATGGATCCTGAGCTTGAAAAAAAACTGAAAGAGAATAAAATATCTTT; AGAGTCGGAATATGAGAAAATCAAAGACTCCAAGTTTGATGACTGGAAGAATATTCGAGG; ACCCAGGCNCTTGGGAAGATCCTGACCTCCTCCAAGGAAGAAATCCAGAAAGCCTTAAGA; CTAAGACAACTTGACTCTGCTGATTCTTTTTTCCTTTTTTTTTTTTTTAAATAAAAATAT; TATTAACTGGACTTCCTAATATATACTTCTATCAAGTGGAAAGGAAATTCCAGGCCCATG; GAAACTTGGATATGGGTAATTTTGATGACAAATAATCTTCACTAAAGGTCATGTACAGGG; TTTTTATACTTTCCCAGCTATTCCATCTGTGGGATGAAAGTAACAATGTTGGGCAAGTTA; TATTTTACACCCTCGAAATAAAAAATGGTGAAACCTGCTCCAAAAACAAAGTCACGTATT; TCCACTTTCCAACTACCCACATTTTCCTTTTTGCATACCCCTTTAGGGCATCATTTGGAA; ATTCATTTCGGGATTTCTGATCTTTGAATTCGGAATCCCAAGGAGGCACATAGGGTTTGG; GAACCCAATTTTCCAGGAAAATAAAGCCGNAATTTTTTTCTCATTTATAAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_016468
• Insert Size: 1640 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016468.3, NP_057552.1
• RefSeq Size: 710 bp
• RefSeq ORF: 321 bp
• Locus ID: 51241
• UniProt ID: Q9P0S2
• Cytogenetics: 14q24.2
• Protein Families: Secreted Protein, Transmembrane
• Summary: Required for the assembly of the mitochondrial respiratory chain complex IV (CIV), also known as cytochrome c oxidase (PubMed:29355485, PubMed:29381136). Promotes the insertion of copper into the active site of cytochrome c oxidase subunit II (MT-CO2/COX2) (PubMed:29355485, PubMed:29381136). Interacts specifically with newly synthesized MT-CO2/COX and its copper center-forming metallochaperones SCO1, SCO2 and COA6 (PubMed:29381136). Probably facilitates MT-CO2/COX2 association with the MITRAC assembly intermediate containing MT-CO1/COX1, thereby participating in merging the MT-CO1/COX1 and MT-CO2/COX2 assembly lines (PubMed:29381136).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).