AMPK gamma 3 (PRKAG3) (NM_017431) Human Untagged Clone

SKU SC114107

PRKAG3 (untagged)-Human protein kinase, AMP-activated, gamma 3 non-catalytic subunit (PRKAG3)

Product Data

• Type: Human Untagged Clone
• Target Symbol: AMPK gamma 3
• Synonyms: AMPKG3; SMGMQTL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_017431 edited; GGCGGCCGCGAATTCGGCACGAGGCCACAGTGTCGCACACTTGCTCTCAGTTGGTCTGGG; GCTGGCCACATGGAGCCCGGGCTGGAGCACGCACTGCGCAGGACCCCTTCCTGGAGCAGC; CTTGGGGGTTCTGAGCATCAAGAGATGAGCTTCCTAGAGCAAGAAAACAGCAGCTCATGG; CCATCACCAGCTGTGACCAGCAGCTCAGAAAGAATCCGTGGGAAACGGAGGGCCAAAGCC; TTGAGATGGACAAGGCAGAAGTCGGTGGAGGAAGGGGAGCCACCAGGTCAGGGGGAAGGT; CCCCGGTCCAGGCCAGCTGCTGAGTCCACCGGGCTGGAGGCCACATTCCCCAAGACCACA; CCCTTGGCTCAAGCTGATCCTGCCGGGGTGGGCACTCCACCAACAGGGTGGGACTGCCTC; CCCTCTGACTGTACAGCCTCAGCTGCAGGCTCCAGCACAGATGATGTGGAGCTGGCCACG; GAGTTCCCAGCCACAGAGGCCTGGGAGTGTGAGCTAGAAGGCCTGCTGGAAGAGAGGCCT; GCCCTGTGCCTGTCCCCGCAGGCCCCATTTCCCAAGCTGGGCTGGGATGACGAACTGCGG; AAACCCGGCGCCCAGATCTACATGCGCTTCATGCAGGAGCACACCTGCTACGATGCCATG; GCAACTAGCTCCAAGCTAGTCATCTTCGACACCATGCTGGAGATCAAGAAGGCCTTCTTT; GCTCTGGTGGCCAACGGTGTGCGGGCAGCCCCTCTATGGGACAGCAAGAAGCAGAGCTTT; GTGGGGATGCTGACCATCACTGACTTCATCCTGGTGCTGCATCGCTACTACAGGTCCCCC; CTGGTCCAGATCTATGAGATTGAACAACATAAGATTGAGACCTGGAGGGAGATCTACCTG; CAAGGCTGCTTCAAGCCTCTGGTCTCCATCTCTCCTAATGATAGCCTGTTTGAAGCTGTC; TACACCCTCATCAAGAACCGGATCCATCGCCTGCCTGTTCTTGACCCGGTGTCAGGCAAC; GTACTCCACATCCTCACACACAAACGCCTGCTCAAGTTCCTGCACATCTTTGGTTCCCTG; CTGCCCCGGCCCTCCTTCCTCTACCGCACTATCCAAGATTTGGGCATCGGCACATTCCGA; GACTTGGCTGTGGTGCTGGAGACAGCACCCATCCTGACTGCACTGGACATCTTTGTGGAC; CGGCGTGTGTCTGCACTGCCTGTGGTCAACGAATGTGGTCAGGTCGTGGGCCTCTATTCC; CGCTTTGATGTGATTCACCTGGCTGCCCAGCAAACCTACAACCACCTGGACATGAGTGTG; GGAGAAGCCCTGAGGCAGAGGACACTATGTCTGGAGGGAGTCCTTTCCTGCCAGCCCCAC; GAGAGCTTGGGGGAAGTGATCGACAGGATTGCTCGGGAGCAGGTACACAGGCTGGTGCTA; GTGGACGAGACCCAGCATCTCTTGGGCGTGGTCTCCCTCTCCGACATCCTTCAGGCACTG; GTGCTCAGCCCTGCTGGCATCGATGCCCTCGGGGCCTGAGAAGATCTGAGTCCTCAATCC; CAAGCCACCTGCACACCTGGAAGCCAATGAAGGGAACTGGAGAACTCAGCCTTCATCTTC; CCCCACCCCCATTTGCTGGTTCAGCTATGATTCAGGCTTCTTCAGCCCTCCCAAATTGCC; CTTGCCCTACCTGTGCTCCCAGAAGCCCTCGGGCATGCCCAGTGCACCATGGGATGATGA; AATTAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017431 unedited; TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCACAGTGTCGCA; CACTTGCTCTCAGTTGGTCTGGGGCTGGCCACATGGAGCCCGGGCTGGAGCACGCACTGC; GCAGGACCCCTTCCTGGAGCAGCCTTGGGGGTTCTGAGCATCAAGAGATGAGCTTCCTAG; AGCAAGAAAACAGCAGCTCATGGCCATCACCAGCTGTGACCAGCAGCTCAGAAAGAATCC; GTGGGAAACGGAGGGCCAAAGCCTTGAGATGGACAAGGCAGAAGTCGGTGGAGGAAGGGG; AGCCACCAGGTCAGGGGGAAGGTCCCCGGTCCAGGCCAGCTGCTGAGTCCACCGGGCTGG; AGGCCACATTCCCCAAGACCACACCCTTGGCTCAAGCTGATCCTGCCGGGGTGGGCACTC; CACCAACAGGGTGGGACTGCCTCCCCTCTGACTGTACAGCCTCAGCTGCAGGCTCCAGCA; CAGATGATGTGGAGCTGGCCACGGAGTTCCCAGCCACAGAGGCCTGGGAGTGTGAGCTAG; AAGGCCTGCTGGAAGAGAGGCCTGCCCTGTGCCTGNTCCCGCANGCCCCATTTCCCAAGC; TGGGCTGGGATGACGAACTGCGGAAACCCGGCGCCCAGATCTACATGCGCTTCATGCAGG; AGCACACCTGCTACGATGCCATGGCAACTAGCTCCAAGCTAGTCATCTTCGACACCATGC; TGGAGATCAAGAAGGCCTTTCTTGCTCTGGTGCCAACGGTGTGCGGGCAGCCCCTCTATG; GGACAGCAGAAGCANAGCTTGTGGGGATGCTGACATCACTGACTCATCCTGGGCTGATCG; CTACTACAGGTCCCCCTGTTCCGATCTTTGAGATTGACACATAAGATTGAACCTGGAGGG; AAATCTACTGCAAGGCTGTTCAAGCCTCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_017431 unedited; GCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCA; AAAAAAACGGCACTTGGGATTTTTATCCATTTTCCTTCTAGTCTTTGAATTTAAAAAGCA; CAAGTCTCTTGCCACAAGTTCAGGTCAAATGACTGCAGCATTTTGAGTGAAACCAATAAA; ATCTAAGTTCATGGATTGGTCCTTTTGTTTTGTTTTCTCAATTGCTCATTCTCAGGGGTC; CATATACAAGGCCATCCATCCCTGGAGACTAAAAACATGGCTCACTGCGAGAGCTAAGGA; GTTTTCTGGACAAAAATGGAGGGAGGACTCCCCACCTCCAACAGGCAGTTCTATAAATAA; CGGCTGCTCCATGGTGGCCCCTGAAATGCAGCATTTCAACAGCCTTGAAATGACAAACCC; TGGAGAGTAGGAGGGCAGTGGCGACCTTGCTGGGAACAACAGCTGGGACTCCCAAGAGCT; GAAAGCTGAACCACTAGGTTGCCCAAAAACCGAGGAATCCAAAAACCCTGACTAAACCCA; CTGGGGCCATCCCACCCATTTCAGCGGGAAGGGGATGGGCGGCCCTGGAGCCCACATGGC; CCTGGGGCTATCCTAACGCCTCTGGCTTAAGGACCCTTCATCTTTGACCAACCGTTCCCC; TTTATTCCATCTCCCAGCCGCCTGCCCCTCCCCAGGCCTCTGGGCCCCCAGCCCTGCCAG; GCCCTTACGACGGCCTTAAATCCTCCACCTCCTCTCCCTCTAAGCGTGCTCGGCCATACC; ATCGCCTCTATCCACCATCCCTATTACGTCCCCACCTCTTCCGCCTCTTTCCTAGCCCTT; ATTACTTCCCAACCTCTATCTATCACCATACCATTATCATACCTCTCTCCCCTTCCCACA; ATTTTTCCCCTCACTTACCCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_017431
• Insert Size: 2560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_017431.2, NP_059127.2
• RefSeq Size: 2299 bp
• RefSeq ORF: 1470 bp
• Locus ID: 53632
• UniProt ID: Q9UGI9
• Cytogenetics: 2q35
• Domains: CBS
• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway
• Summary: The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. It is dominantly expressed in skeletal muscle. Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle. [provided by RefSeq, Jul 2008]