NUP54 (NM_017426) Human Untagged Clone

SKU SC114106

NUP54 (untagged)-Human nucleoporin 54kDa (NUP54)

Product Data

• Type: Human Untagged Clone
• Target Symbol: NUP54
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114106 sequence for NM_017426 edited (data generated by NextGen Sequencing); ATGGCCTTCAATTTTGGGGCTCCCTCGGGCACCTCCGGTACCGCTGCAGCCACCGCGGCC; CCCGCGGGTGGGTTTGGAGGATTTGGGACAACATCTACAACTGCAGGTTCTGCATTCAGC; TTTTCTGCCCCAACTAACACAGGCACTACTGGACTCTTTGGTGGTACTCAGAACAAAGGT; TTTGGATTTGGTACTGGTTTTGGCACAACAACGGGAACTAGTACTGGTTTAGGTACTGGT; TTGGGAACTGGACTGGGATTTGGAGGATTTAATACACAGCAGCAGCAGCAAACTACATTA; GGTGGTCTCTTCAGTCAGCCTACACAAGCTCCTACCCAGTCCAACCAGCTGATAAATACT; GCGAGTGCTCTTTCTGCTCCAACGCTGTTGGGAGATGAGAGAGATGCTATTTTGGCAAAA; TGGAATCAACTGCAGGCCTTTTGGGGAACAGGAAAAGGGTATTTCAACAATAATATTCCG; CCAGTGGAATTCACACAAGAAAATCCCTTTTGCCGATTTAAGGCAGTAGGTTATAGTTGC; ATGCCCAGTAATAAAGATGAAGATGGGCTAGTGGTTTTAGTTTTCAACAAAAAAGAAACA; GAGATTCGAAGCCAACAACAACAGTTGGTAGAATCATTGCATAAAGTTTTGGGAGGAAAC; CAGACCCTTACTGTAAATGTAGAGGGCACTAAAACATTGCCAGATGATCAGACAGAAGTT; GTTATTTATGTTGTTGAGCGTTCGCCAAGTGGTACTTCAAGAAGAGTTCCAGCTACAACG; CTATATGCCCATTTTGAACAAGCCAATATAAAAACACAATTGCAGCAACTTGGTGTAACC; CTTTCTATGACTAGAACAGAACTTTCTCCTGCACAGATCAAACAGCTTTTACAGAATCCT; CCTGCTGGTGTTGATCCTATTATCTGGGAACAGGCCAAGGTAGATAACCCTGATTCTGAA; AAGTTAATTCCTGTACCAATGGTGGGTTTTAAGGAACTTCTCCGAAGACTGAAGGTTCAA; GATCAGATGACTAAGCAGCATCAAACCAGATTAGATATCATATCTGAAGATATTAGTGAG; CTACAAAAGAATCAAACTACATCTGTAGCCAAAATTGCACAATACAAGAGGAAACTCATG; GATCTTTCCCATAGAACTTTACAGGTCCTAATCAAACAGGAAATTCAAAGGAAGAGTGGT; TATGCCATTCAGGCTGATGAAGAGCAGTTGCGAGTTCAGCTGGATACGATTCAGGGTGAA; CTAAATGCACCTACTCAGTTCAAGGGCCGACTAAATGAATTGATGTCTCAAATCAGGATG; CAGAATCATTTTGGAGCAGTCAGATCTGAAGAAAGGTATTACATAGATGCAGATCTGTTA; CGAGAAATCAAGCAGCATTTGAAACAACAACAGGAAGGCCTTAGCCATTTGATTAGCATC; ATTAAAGACGATCTAGAAGATATAAAGCTGGTCGAACATGGATTGAATGAAACCATCCAC; ATCAGAGGTGGTGTCTTTAGTTGA; ; Clone variation with respect to NM_017426.2; 749 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017426 unedited; GTTANGATTTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGCGCGAGCTTTCGCG; ACATGGGCCTTCAATTTTGGGGCTCCCTCGGGCACCTCCGGTACCGCTGCAGCCACCGCG; GCCCCCGCGGGTGGGTTTGGAGGATTTGGGACAACATCTACAACTGCAGGTTCTGCATTC; AGCTTTTCTGCCCCAACTAACACAGGCACTACTGGACTCTTTGGTGGTACTCAGAACAAA; GGTTTTGGATTTGGTACTGGTTTTGGCACAACAACGGGAACTAGTACTGGTTTAGGTACT; GGTTTGGGAACTGGACTGGGATTTGGAGGATTTAATACACAGCAGCAGCAGCAAACTACA; TTAGGTGGTCTCTTCAGTCAGCCTACACAAGCTCCTACCCAGTCCAACCAGCTGATAAAT; ACTGCGAGTGCTCTTTCTGCTCCAACGCTGTTGGGAGATGAGAGAGATGCTATTTTGGCA; AAATGGAATCAACTGCAGGCCTTTTGGGGAACAGGAAAAGGGTATTTCAACAATAATATT; CCGCCAGTGGAATTCACACAAGAAAATCCCTTTTGCCGATTTAAGGCAGTAGGTTATAGT; TGCATGCCCAGTAATAAAGATGAAGATGGGCTAGTGGTTTTAGTTTTCAACAAAAAAGAA; ACAGAGATTCGAAGCCAACAACAACAGTTGGTAGAATCATTGCATAAAGTTTTGGGAGGA; AACCACACCCTTACTGTAAATGTAGAGGGCACTAAAACATTGCCAGATGATCAGACAGAA; GTTGTTATTTATGTTGTTGAGCGTTCGCCAAGTGGTACTTCAAGAAGAGTTCAGCTACAA; CGCTATATGCCCCATTTTGACAACCCATATAAAAACACAATTGCCACCACTCTGCTGTAC; CCCTTTCTATGACTAGAACAGAACTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_017426 unedited; GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTAATTACAAATTAAAAATTTATTTATAAGTTTTCATTTTTTTAAAAATAAAA; AGGCTTAAAAAACAAAATGGTTGCAAAAATGGTTAAGTAGTTTTGTTATATCCTAATTGC; TTAGGGGGGCTCTGTAAATTCCAAATAAGTAAAATAATATAAAAATACATTTTCATTTTT; TTATAGAACAAAAACAAAACATTAAATGCTTTTGGATTTTTTTTACTCCTCCCACAGATG; AGTTCACAAATACAAAAACTGGTGTAATTTATACTCAAGTACAAATCTCCACAGCCCAGG; ATTATAATTTGGTCTGTTATTGGCCAAGAAAATTTTTATTTTCCCTTGGTTGGGAAACAA; ATCCCGGGTTTTGGGGAATTTTTTTTTAAACCCATTTTTTTTTTAAAAAAACGGTTGGGA; AAAAGCCCCCCCCCCCCCCCCTTGGGGGGAAACCCCCCCTTTTTAAAAAAAAACCCCCTT; TTATAAAATTTTTTAGAGGGTTTTTTTTAGAGGGGGGGGGGTGTTTTTTTTTTGTGAAAA; AATTTTTTTTATTTTTTTTTAAAACACCCCCCAAAGAGGGTTTTTTTTTTTTTCCCTCCC; GGGGGGTTTTTTTTTGGGGGGGGGGGGGGAGAAAAAAAAAAAACTCCCCCCCCCCCCCCC; CGGGGGGGGGGGAAAAACCACCCCCCCCCCGGGGGGGGGGGGGCCTCCTCCCCCCCCCCC; CCCTTTCCTTTTTTTTTGTTGGGAGGGGAGGGGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_017426
• Insert Size: 2240 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_017426.2, NP_059122.2
• RefSeq Size: 2358 bp
• RefSeq ORF: 1524 bp
• Locus ID: 53371
• UniProt ID: Q7Z3B4
• Cytogenetics: 4q21.1
• Summary: The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. This gene encodes a member of the phe-gly (FG) repeat-containing nucleoporin subset. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013]; Transcript Variant: This variant (1) encodes the longer isoform (1).